Isolates, Antimicrobial Susceptibility Profiles and Multidrug Resistance of Bacteria Cultured from Pig Submissions in New Zealand

Data on the scope of bacterial pathogens present and the frequency of antimicrobial resistance (AMR) in New Zealand’s pigs are limited. This study describes bacterial isolates, antimicrobial susceptibility data, and multidrug resistance (MDR; resistance to ≥3 antimicrobial classes) from New Zealand pig submissions. Porcine test data from June 2003 to February 2016 were obtained from commercial veterinary pathology laboratory records. In total, 470/477 unique submissions resulted in bacterial growth, yielding 779 isolates. Sample type was recorded for 360/477 (75.5%); lung (79/360; 21.9%), faecal (61/360; 16.9%) and intestinal (45/360; 12.5%) were most common. The most common isolates were Escherichia coli (186/779, 23.9%), Actinobacillus pleuropneumoniae (43/779; 5.5%), Streptococcus suis (43/779; 5.5%), unidentified Campylobacter spp. (38/779; 4.9%), alpha haemolytic Streptococci (32/779; 4.1%), coagulase negative Staphylococcus spp. (26/779; 3.3%), and Pasteurella multocida (25/779; 3.2%). Susceptibility results were available for 141/779 (18.1%) isolates from 62/470 (13.2%) submissions. Most were susceptible to trimethoprim-sulphonamide (75/81; 92.6%), but fewer were susceptible to penicillin (37/77; 48.1%), tilmicosin (18/43; 41.9%), or tetracyclines (41/114; 36.0%). No susceptibility data were available for Salmonella spp., Campylobacter spp., or Yersinia spp. isolates. MDR was present in 60/141 (42.6%) isolates. More data on sample submission drivers, antimicrobial drug use, and susceptibilities of important porcine bacterial isolates are required to inform guidelines for prudent antimicrobial use, to reduce their prevalence, human transmission, and to minimise AMR and MDR.

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Bacterial isolates, antimicrobial susceptibility and multidrug resistance in cultures from samples collected from beef and pre-production dairy cattle in New Zealand (2003–2016)

New Zealand Veterinary Journal ◽

10.1080/00480169.2019.1605943 ◽

2019 ◽

Vol 67 (4) ◽

pp. 180-187 ◽

Cited By ~ 4

Author(s):

KE Lawrence ◽

L Wakeford ◽

LJ Toombs-Ruane ◽

C MacLachlan ◽

H Pfeffer ◽

...

Keyword(s):

Multidrug Resistance ◽

New Zealand ◽

Dairy Cattle ◽

Antimicrobial Susceptibility ◽

Bacterial Isolates

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Isolates, antimicrobial susceptibility profiles and multidrug resistance of bacteria cultured from samples collected from sheep in New Zealand (2003–2016)

New Zealand Veterinary Journal ◽

10.1080/00480169.2020.1789517 ◽

2020 ◽

Vol 69 (1) ◽

pp. 20-26 ◽

Cited By ~ 1

Author(s):

CB Riley ◽

H Pfeffer ◽

C MacLachlan ◽

L Wakeford ◽

IR Gibson ◽

...

Keyword(s):

Multidrug Resistance ◽

New Zealand ◽

Antimicrobial Susceptibility ◽

Susceptibility Profiles

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Prevalence and Antimicrobial Susceptibility of Campylobacter spp. in Oklahoma Conventional and Organic Retail Poultry

The Open Microbiology Journal ◽

10.2174/1874285801408010130 ◽

2014 ◽

Vol 8 (1) ◽

pp. 130-137 ◽

Cited By ~ 24

Author(s):

Aneesa Noormohamed ◽

Mohamed K Fakhr

Keyword(s):

Multidrug Resistance ◽

Antimicrobial Resistance ◽

Foodborne Pathogens ◽

Antimicrobial Susceptibility ◽

Grocery Stores ◽

Campylobacter Spp

Campylobacteris one of the most important foodborne pathogens that cause bacterial gastroenteritis.This study was conducted to investigate the prevalence and antimicrobial resistance ofCampylobacterin conventional and organic retail poultry samples purchased from grocery stores in Tulsa, Oklahoma.One hundred and fifty six chilled retail chicken samples (85 conventional and 71 organic) and 65 chilled retail conventional turkey samples were collected in this study. The prevalence ofCampylobacterin the conventional chicken samples 32/85 (38%) was higher than in the organic ones 21/71 (30%). The prevalence ofCampylobacterin the conventional turkey samples was 11/65 (17%). Of the 53 positive chicken samples, 42 wereC. jejuni, 8 wereC. coliand three isolates were contaminated with both species. Of the 11 positive turkey samples, 8 containedC. jejuniand 3 harboredC. coliisolates. The antimicrobial susceptibility of one hundred and forty nine recoveredCampylobacterisolates (130 chickens and 19 turkeys) towards sixteen antimicrobials was determined. The majority of the recovered turkey isolates (13/19) showed resistance to more than 7 antimicrobials while most of the recovered chicken ones (82/130) were resistant to 5 to 7 antimicrobials. Multidrug resistance was not limited to isolates from conventional sources but was also available in isolates of an organic background and was generally lower inC. jejuniisolates when compared to theC. coliones.

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Isolation, Identification, and Antimicrobial Susceptibility of Bacteria Associated with Waterpipe Contaminants in Selected Area of Saudi Arabia

BioMed Research International ◽

10.1155/2017/8042603 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Mohammed Alaidarous ◽

Meshal Alanazi ◽

Ahmed Abdel-Hadi

Keyword(s):

Saudi Arabia ◽

Antimicrobial Susceptibility ◽

Multidrug Resistant ◽

Health Concern ◽

Contamination Rate ◽

Bacterial Isolates ◽

Biochemical Tests ◽

Susceptibility Data ◽

Public Health Professionals ◽

Vitek 2

This study highlights the level of microbial contamination of waterpipe components in selected area of Saudi Arabia and the resistance of selected bacteria to different antibiotics was determined. A series of biochemical tests, microscopic examination, and screening on Vitek 2 compact (bioMérieux Inc., USA) system were done to characterize the bacterial isolates. Out of 132 samples investigated, 7 mouthpiece samples and 48 water bowl samples showed positivity on culture. The percentage of contamination rate was higher in water bowl (69.69%) than in mouthpieces (10.6%) for all selected areas. A total of 55 bacterial isolates were identified which included Gram-negative (28) and Gram-positive (27) bacteria. Antimicrobial susceptibility data showed more resistance to bacteria isolated from water bowl than bacteria isolated from mouthpiece. In addition, one isolate which was confirmed as methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae was resistant to antibiotics which are commonly used to treat pneumonia. Water bowl of waterpipe instrument is significantly contaminated with different bacterial pathogens including multidrug-resistant and pneumonia causing bacteria, which are a real health concern among waterpipe smokers. The presented data could assist public health professionals to raise the concerns regarding cleaning practices of waterpipe components and highlights the risk posed among the waterpipe smokers.

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110. The prevalence ofE. coli, Salmonella spp.,Campylobacter spp., VRE and MRSA in dogs and their antimicrobial susceptibility

Research in Veterinary Science ◽

10.1016/s0034-5288(02)90114-1 ◽

2002 ◽

Vol 72 ◽

pp. 40

Author(s):

J.S. Wilson ◽

N.J. Williams ◽

M. Bennett ◽

C.A. Hart

Keyword(s):

Antimicrobial Susceptibility ◽

Salmonella Spp ◽

Campylobacter Spp

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Incidence and antimicrobial susceptibility of Salmonella, Listeria, and Campylobacter spp. in raw “souvlaki” marketed in Thessaloniki (Northern Greece)

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15859 ◽

2018 ◽

Vol 66 (3) ◽

pp. 161 ◽

Cited By ~ 1

Author(s):

N. SOULTOS (Ν. ΣΟΥΛΤΟΣ) ◽

E. IOSSIFIDOU (Ε. ΙΩΣΗΦΙΔΟΥ) ◽

E. PSOMAS (Ε. ΨΩΜΑΣ) ◽

Z. TZIKAS (Ζ. ΤΖΗΚΑΣ) ◽

T. LAZOU (Θ. ΛΑΖΟΥ)

Keyword(s):

Antimicrobial Susceptibility ◽

Thermal Processing ◽

Antimicrobial Agents ◽

Meat Product ◽

Diffusion Method ◽

Salmonella Spp ◽

Northern Greece ◽

Food Borne ◽

Food Borne Infections ◽

Campylobacter Spp

Souvlaki is a popular Greek meat product consisting of small chunks or cubes of pork threaded on a small wooden or metal skewer. In the present study, 105 samples of raw pork souvlaki obtained from retail shops in Thessaloniki (Northern Greece) were screened for the incidence of Salmonella, Listeria, and Campylobacter and their susceptibility to various antimicrobial agents; serotyping of the isolates was also performed. Of the samples tested, 1.9% were positive for Salmonella and yielded 3 serovars (S. SaintPaul, S. Fyris and S. Typhimurium); 31.4% proved positive for Listeria spp. with 6.7% yielding L. monocytogenes isolates belonged to molecular serogroups 2 (serotypes 1/2c and 3c) and 4 ( serotypes 4b, 4d, and 4e). Campylobacter spp. were not detected in any of the samples tested. The antimicrobial susceptibility to various antimicrobial agents of 11 Salmonella strains and 7 L. monocytogenes strains was also determined by disc diffusion method. Salmonella spp. were susceptible to a panel of 12 antibiotics but displayed intermediate resistance to tetracycline. L. monocytogenes isolates were resistant to nalidixic acid and ceftriaxone, partly resistant to clindamycin and cefotaxime, but sensitive to all antibiotics commonly used in veterinary and human listeriosis. Our findings indicate that souvlaki could be a potential vehicle of food borne infections due to strains of L. monocytogenes and Salmonella spp. in the case of inadequate thermal processing. In addition, good hygienic practices must be applied to avoid cross-contamination during preparation or handling of the product.

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Pathologic and microbiologic aspects of pet psittacine infected by Escherichia coli and Salmonella Typhimurium

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2017000400012 ◽

2017 ◽

Vol 37 (4) ◽

pp. 379-384

Author(s):

Raul A.S. Siqueira ◽

William C. Maciel ◽

Ruben H. Vasconcelos ◽

Windleyanne G.A. Bezerra ◽

Elisângela S. Lopes ◽

...

Keyword(s):

Escherichia Coli ◽

Multidrug Resistance ◽

Salmonella Typhimurium ◽

Antimicrobial Susceptibility ◽

Inflammatory Infiltrate ◽

State University ◽

Antimicrobial Susceptibility Test ◽

Salmonella Spp ◽

E Coli

ABSTRACT: The role of Escherichia coli in healthy microbiota of psittacine is controversial, and the presence of Salmonella sp. indicates possible disease. Therefore, this study aimed to identify the presence of E. coli and Salmonella spp. in a psittacine pet that died in Fortaleza, Brazil, correlating pathogenicity aspects of the isolates through the evaluation of lesions and antimicrobial susceptibility. Psittacine pets sent to the Laboratory of Ornithological Studies, State University of Ceará, that died in 2014 and 2015 were necropsied. Fragments of liver, kidneys, intestine, lung, heart, spleen and brain were collected for microbiological and histopathological analyses. Scores were attributed to lesions and isolated strains submitted to antimicrobial susceptibility test. From the seventy necropsied birds, nineteen were positive for E. coli and one for Salmonella Typhimurium. Congestive lesions and lymphoplasmocitic inflammatory infiltrate were observed varying from light to moderate and were the main findings. In the analyzed strains, multidrug resistance against different groups of antibiotics was observed. In conclusion, according to the results, E. coli strains and the Salmonella Typhimurium isolate produced significant lesions in the psittacine pets, and multidrug resistance may hinder treatments with antibiotics used in avian pet medicine.

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