scholarly journals Influence of Strain and Diet on Urinary pH in Laboratory Mice

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 702
Author(s):  
Linda F. Böswald ◽  
Dana Matzek ◽  
Ellen Kienzle ◽  
Bastian Popper

Acid base homeostasis and urine pH is influenced by the dietary cation anion balance (DCAB) in many species. Here, a negative DCAB acidifies the urine, while higher DCABs alkalize the urine. The dimension of the DCAB effect can be species-specific, because of differences in urine buffer systems. The aim of the present study was to describe the response of laboratory mice to diets with different DCAB. We used 8-week-old wildtype male mice of the C57Bl/6J inbred strain and CD1 outbred stock. Three groups (n = 15 animals/group) were formed and fed standard diet A for adaptation. For the 7-week feeding trial, mice were either kept on diet A (DCAB −7 mmol/kg dry matter (DM) or switched to diet B (246 mmol/kg DM) or C (−257 mmol/kg DM). Urine pH was measured weekly from a pooled sample per cage. There was a significant difference in the basal urine pH on diet A between C57Bl6/J and CD1 mice. The shift in urine pH was also significantly different between the two groups investigated.

1953 ◽  
Vol 43 (2) ◽  
pp. 166-177 ◽  
Author(s):  
T. L. Dodsworth ◽  
W. H. McK. Campbell

1. A feeding trial is described including four groups of cattle fed on grades I, II and III silages and on swedes, straw and oats respectively.2. The live-weight gains made are recorded, together with the rate of dry-matter intake.3. The efficiency of the dry matter of the diets was calculated.4. All three grades of silage produced a significantly greater rate of fattening than swedes plus straw and oats. There was no significant difference between the fattening values of the three grades of silage.5. The dressing percentages found for 100 silagefed and 71 swede-fed cattle are given. The swedefed cattle yielded a greater average dressing percentage.6. It is suggested that the higher dressing percentage found for root-fed cattle as compared with silage-fed cattle can be attributed to the more rapid elimination of dietary water from the body in rootfed cattle.7. An animal behaviour study is described, the time spent by cattle on the four diets, eating, cudding and lying down, being determined.8. Cattle fed on grass silage spent longer on feeding plus cudding than swede-fed cattle.9. An experiment is described designed to determine the effect of the dry-matter percentage of the diet on the dry-matter intake of ruminants.10. The results indicate that when ruminants are subsisting on a diet consisting solely of succulents, the dry-matter intake is depressed when the dry-matter percentage of the diet falls.


2015 ◽  
Vol 42 (2) ◽  
pp. 151-157
Author(s):  
A. B. I. Udedibie ◽  
F. I. Peter-Nwachukwu ◽  
H. O. Obikaonu

A 5-week experiment was conducted to investigate the effects of qualitative feed restriction through the use low-quality diet on finisher broiler performance, and to determine if sufficient compensatory growth can be achieved at that phase of broiler production. Seventy two (72) 4 weeks old broiler chicks of Anak strain weighing of the average 1.0kg were randomly distributed into two groups, A and B, using completely randomized design. Each group was replicated 3 times with 12 broiler chicks per replicate and each replicate housed in a pen measuring 1.5 m x 2 m. Broilers in group A were given a standard broiler finisher diet continuously for 5 weeks while broilers in group B received a low-quality cheap diet based mainly on palm kernel cake and wheat offal for the first two weeks and then placed on the standard diet for the remaining three weeks of the trial. The birds were weighed at the beginning of the trial and weekly thereafter. At the end of the feeding trial, 4 birds were randomly selected from each group, weighed, sacrificed, de-feathered, eviscerated and their internal organs as well as the abdominal fat, weighed. There was no significant difference in feed intake between the groups (P>0.05) at the end of the trial but the group on the high fibre low-quality diet consumed significantly (P<0.05) more feed during the 2 weeks. Daily body weight gain of the birds on the low-quality diet was lower than that of their counterparts in the control diets in the first 2 weeks but remarkably and progressively increased up to the end of the feeding trial. There was, however, no significant difference between the groups (P>0.05) in the final body weights. There were no significant differences in internal organ weights of the groups (P>0.05). However, abdominal fat weight was significantly (P<0.05) reduced by qualitative feed restriction. Feed cost of production (N/kg weight gain) were N348.69 and N343.78 for the control and qualitative feed restricted groups, respectively. The results have shown that sufficient compensatory growth can be achieved in broiler production the period of production is just 5 weeks.


2016 ◽  
Vol 2 (2) ◽  
pp. 83-90 ◽  
Author(s):  
V. Maurer ◽  
M. Holinger ◽  
Z. Amsler ◽  
B. Früh ◽  
J. Wohlfahrt ◽  
...  

Insects will likely play an important role as protein sources for livestock in the future. Many insect species are able to convert materials not suitable for human nutrition – or even waste – into valuable protein with a favourable amino acid composition for poultry and other livestock. A feeding trial with partly de-fatted meal of dried Hermetia illucens larvae (Hermetia meal) reared on vegetarian by-products of the pasta and convenience food industry was carried out in small groups of Lohmann Selected Leghorn laying hens (four rounds, 10 hens/round). Experimental diets H12 and H24 contained 12 and 24 g/100 g Hermetia meal replacing 50 or 100% of soybean cake used in the control feed, respectively. After three weeks of feeding experimental diets, there were no significant differences between feeding groups with regard to performance (egg production, feed intake). There was a tendency (P=0.06) for lower albumen weight in the H24 group; yolk and shell weights did not differ. No mortality and no sign of health disorders occurred. Plumage as well as wound scores remained stable during the feeding period and did not differ between treatments. Dry matter of faeces increased with increasing proportions of Hermetia meal in the diet, with a significant difference between H24 and the control (P=0.03). An increase of black faecal pads was observed in the H12 and H24 groups. Overall, these results suggest Hermetia meal can be a valuable component of layer diets. However, insect meal production still has to become economically more viable through upscaling production and, especially, legislative issues have to be solved.


2019 ◽  
Vol 49 ◽  
Author(s):  
José Herilalao Andriarimalala ◽  
Claude Cocou Kpomasse ◽  
Paulo Salgado ◽  
Noroseheno Ralisoa ◽  
Jayraman Durai

ABSTRACT Feed shortage during the dry season in the highlands of Madagascar negatively impacts the dairy cattle productivity, as well as the farmers’ income. Bamboo can produce a high quantity of biomass and could be an alternative way to increase the fodder supply for cattle. The present study aimed to assess the compositional information and the optimal rate of bamboo as fodder for dairy cattle. Leaf samples from nine bamboo species were collected to determine their chemical composition and nutritive value. A feeding experiment was also conducted using ten dairy cows, over ten weeks. Bamboo leaves were mixed with maize silage in five proportions: SIL0:BAM100, SIL25:BAM75, SIL50:BAM50, SIL75:BAM25 and SIL100:BAM0. The contents of dry matter, total ash and crude protein in the bamboo leaves were, respectively, 44.5-64.6 %, 6.68-18.5 % and 7.71-15.4 %. In the feeding trial, the dry matter intake of bamboo leaves was 1.6-7.1 kg per day, with an average of 4.8 kg per day. The dry matter apparent digestibility of bamboo leaves was 37.4-56.4 %. The milk production reached 13.6-14.4 L per cow, per day, but there was no significant difference concerning the rate of bamboo leaves in the mixed diet (p > 0.05). The introduction of bamboo leaves into the ruminants diet did not affect their milk production.


Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4399
Author(s):  
Michael S. Stone ◽  
Berdine R. Martin ◽  
Connie M. Weaver

Potassium supplementation has been associated with reduced urinary calcium (Ca) excretion and increased Ca balance. Dietary interventions assessing the impact of potassium on bone are lacking. In this secondary analysis of a study designed primarily to determine blood pressure effects, we assessed the effects of potassium intake from potato sources and a potassium supplement on urinary Ca, urine pH, and Ca balance. Thirty men (n = 15) and women (n = 15) with a mean ± SD age and BMI of 48.2 ± 15 years and 31.4 ± 6.1 kg/m2, respectively, were enrolled in a cross-over, randomized control feeding trial. Participants were assigned to a random order of four 16-day dietary potassium interventions including a basal diet (control) of 2300 mg/day (~60 mmol/day) of potassium, and three phases of an additional 1000 mg/day (3300 mg/day(~85 mmol/day) total) of potassium in the form of potatoes (baked, boiled, or pan-heated), French fries (FF), or a potassium (K)-gluconate supplement. Calcium intake for all diets was approximately 700–800 mg/day. Using a mixed model ANOVA there was a significantly lower urinary Ca excretion in the K-gluconate phase (96 ± 10 mg/day) compared to the control (115 ± 10 mg/day; p = 0.027) and potato (114 ± 10 mg/day; p = 0.033). In addition, there was a significant difference in urinary pH between the supplement and control phases (6.54 ± 0.16 vs. 6.08 ± 0.18; p = 0.0036). There were no significant differences in Ca retention. An increased potassium intake via K-gluconate supplementation may favorably influence urinary Ca excretion and urine pH. This trial was registered at ClinicalTrials.gov as NCT02697708.


2020 ◽  
Vol 17 (2) ◽  
pp. 192
Author(s):  
RONALDO LAU ◽  
SULISTIANA PRABOWO ◽  
RIAMI RIAMI

<p align="justify"><strong>ABSTRACT</strong><strong></strong></p><p align="justify"><strong>Background</strong>: High fat diet increase the absorption of lipid in the intestinum, that can lead to increase LDL cholesterol level in the blood. Sea grapes extract (<em>Caulerpa racemosa</em>) contains antioxidant polyphenolic group that can reduce MTP and ACAT-2 in the body that can decrease LDL cholesterol level in the blood.The purpose of this study is to know the effect of sea grapes extract  on decreasing LDL cholesterol of white male Wistar rats (<em>Rattus norvegicus</em>) fed with high fat diet.</p><p align="justify"><strong>Method</strong>:  24 white male Wistar rats, that divided into 3 groups: 1) group of rats fed with standard diet for 28 days; 2) group of rats fed with high fat diet for 28 days; 3) group of rats fed with high fat diet for 28 days and given 10 gram/kg body weight/day of sea grapes extract on 15<sup>th</sup>-28<sup>th</sup> days. Then the blood LDL cholesterol level measured on the 29<sup>th</sup> day.</p><p align="justify"><strong>Result :</strong> One-Way ANOVA Test showed there was significant difference (p=0.004) of LDL level between the group of rats fed with standard diet (12.37 mg/dl) compared to group of rats fed with high fat diet (17.87 mg/dl). There was significant difference (p=0.001) of LDL level between the group of rats fed with high fat diet (17.87 mg/dl) compared to group of rats fed with high fat diet and sea grapes extract (10.12 mg/dl).</p><p align="justify"><strong>Conclusion: </strong>high fat diet significantly increase blood LDL cholesterol level and sea grapes extract (<em>Caulerpa racemosa</em>) significantly decrease blood LDL cholesterol level.</p><p align="justify"> </p><p align="justify"><strong>Keywords :</strong>Sea grapes extract, LDL cholesterol, high fat diet</p>


2017 ◽  
Vol 4 (03) ◽  
Author(s):  
SAMBORLANG K. WANNIANG ◽  
A. K. SINGH

A field experiment was conducted during kharif 2011 on experimental farm of the College of Post Graduate Studies (CAU–Imphal), Umiam (Meghalaya) to evaluate the effect of integration of green manuring, FYM and fertilizers as integrated nutrient management (INM) practices on growth and developmental behaviour of quality protein maize cultivar QPM 1. The data revealed that comparatively higher amount of primary nutrients were added in green manured maize plots in comparison to non green manured treatments. Green manuring also left a positive response on plant height, CGR, RGR leaf area, and dry matter accumulation in plants though the difference between green manured and non-green manured treatments was at par. Treatments 75 % RDF + 5 t FYM ha-1, 50 % RDF + 7.5 t FYM ha-1, 100 % RDF ha-1 and 75 % RDF + 2.5 t FYM ha-1 recorded significantly higher values of all the above said growth parameters over 50 % RDF + 5 t FYM ha-1 and control treatments. At all stages of observations, the maximum dry matter was associated with RDF (recommended doses of fertilizers) which was at par with 75 % RDF + 5 t FYM ha-1, but significantly higher over the plant dry weight recorded from all remaining treatments. A Significant difference in CGR at 30 – 60 and 60 – 90 DAS stage and in RGR at 90 DAS - harvest stage was observed due to various combinations of recommended dose of fertilizer with different doses of FYM. Number of days taken to attain the stages of 50% tasselling, silking and maturity did not differ significantly due to green manuring. However, treatment 75 % RDF + 5 t FYM ha-1 took significantly lesser number of days for these stages than other treatment combinations. The superiority of the treatment 75 % RDF + 5 t FYM ha-1 indicated a possibility of substituting 25% of RDF with 5 t FYM ha-1 without any loss in dry matter accumulation in plants of the quality protein hybrid maize in mid-hill ecosystems of Meghalaya.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaodan Wang ◽  
Yaliang Wang ◽  
Yuping Zhang ◽  
Jing Xiang ◽  
Yikai Zhang ◽  
...  

AbstractDetermination of the optimal fertilization method is crucial to maximize nitrogen use efficiency and yield of different rice cultivars. Side-deep fertilization with controlled-release nitrogen, in conjunction with machine transplanting and subsequent topdressing, was applied to Indica–japonica hybrid rice ‘Yongyou1540’ (YY1540) and indica hybrid rice ‘Tianyouhuazhan’ (TYHZ). Four nitrogen treatments were applied in 2018 and 2019: traditional nitrogen application with quick-release nitrogen (T1), single-dose deep fertilization at transplanting with 100% controlled-release nitrogen (T2), and deep fertilization of 70% controlled-release nitrogen and topdressing of 30% quick nitrogen at tillering (T3), or at panicle initiation (T4). Side-deep fertilization reduced the fertilizer application frequency without causing yield loss, T4 enhanced the yield of YY1540 by increasing the number of productive tillers and number of spikelets per panicle compared with T1, T2 and T3. The yield of TYHZ showed no significant difference among treatments. The T4 treatment decreased the number of tillers at the tilling peak stage and increased the percentage productive tillers and number of differentiated spikelets. Compared with the other treatments, T4 increased dry matter accumulation and leaf area index during panicle initiation and grain ripening, and contributed to enhanced nitrogen uptake and nitrogen utilization in YY1540. On average, nitrogen uptake and utilization in YY1540 were highest in T4, but no significant differences among treatments were observed in TYHZ. Dry matter accumulation and nitrogen uptake from panicle initiation to heading of YY1540 were correlated with number of spikelets per panicle, but no significant correlations were observed for TYHZ. Supplementary topdressing with quick-release nitrogen at the panicle initiation stage was required to increase yield of indica–japonica hybrid rice, whereas single-dose deep fertilization with controlled-release nitrogen is satisfactory for the indica hybrid cultivar.


2021 ◽  
Vol 14 ◽  
pp. 194008292110103
Author(s):  
Patrick Jules Atagana ◽  
Eric Moïse Bakwo Fils ◽  
Sevilor Kekeunou

We aimed to assess how bats are affected by habitat transformation by comparing bat assemblages in four habitat types: primary forest, secondary forest, cocoa plantations and human habitations in the Dja Biosphere Reserve of southern Cameroon. Bats were sampled in the four habitat types using mist nets. During 126 nights, a total of 413 bats were captured, belonging to four families, 16 genera and 24 species. Ninety three individuals (17 species) were captured in the primary forest, followed by plantations (105 individuals, 14 species), human habitations (159 individuals, 10 species), and secondary forest (55 individuals, eight species). Megaloglossus woermanni was recorded in all the four habitats, and was the most abundant species (105 individuals). The analysis of bat assemblage between habitat types showed a statistically significant difference in species composition. The distribution of the six most abundant species ( Epomops franqueti, Megaloglossus woermanni, Rousettus aegyptiacus, Dohyrina cyclops, Hipposideros cf. caffer and Hipposideros cf. ruber) was influenced by habitat types. Our results suggest that the decrease in species richness observed in disturbed habitats may be due to habitat perturbations of primary forest habitats. Therefore, it is important to examine the effects of habitat conversion at species level, as responses are often species-specific.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zahra Barati ◽  
Mina Iravani ◽  
Majid Karandish ◽  
Mohammad Hosein Haghighizadeh ◽  
Sara Masihi

Abstract Background Gestational diabetes is the most common medical complication in pregnancy, and it has many side effects for the mother and the fetus. The aim of this study was to evaluate the effect of oat bran consumption on gestational diabetes. Methods This study is a randomized clinical trial that was performed on 112 women with gestational diabetes treated with diet. Participants were randomly divided into two groups of 56. Participants in both groups were given a diet for gestational diabetes. In addition to the diet, the intervention group received 30 g of oat bran daily for 4 weeks at lunch and dinner. Tests of fasting blood glucose and two-hour postprandial (2hpp) glucose were taken from both groups: before the intervention, and 2 and 4 weeks after the start of the intervention. Data analysis was performed using SPSS statistical software (version 22) using independent t-test, as well as Chi-square and Mann-Whitney tests. P values less than 0.05 were considered statistically significant. Results There was no statistically significant difference between the two groups in terms of mean blood glucose before the intervention, while 2 and 4 weeks after the intervention, mean fasting blood glucose and two-hour postprandial (2hpp) glucose decreased significantly in the intervention group compared with the control group (P < 0.001). Conclusion Based on the results of this study, the addition of oat bran to the standard diet for pregnant women with gestational diabetes reduced fasting blood glucose and two-hour postprandial (2hpp) glucose. More detailed studies with higher sample sizes are recommended to prove the effectiveness of this valuable dietary supplement. Trial registration IRCT registration number:IRCT20191220045828N1. Registration date: 2020-04-18. Registered while recruiting.


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