Molecular and Cellular Responses of DNA Methylation and Thioredoxin System to Heat Stress in Meat-Type Chickens

Heat stress (HS) causes molecular dysfunction that adversely affects chicken performance and increases mortality. The responses of chickens to HS are extremely complex. Thus, the aim of this study was to evaluate the influence of acute and chronic exposure to HS on the expression of thioredoxin–peroxiredoxin system genes and DNA methylation in chickens. Chickens at 14 d of age were divided into two groups and reared under either constant normal temperature (25 °C) or high temperature (35 °C) in individual cages for 12 days. Five birds per group at one and 12 days post-HS were euthanized and livers were sampled for gene expression. The liver and Pectoralis major muscle were sampled for cellular analysis. mRNA expression of thioredoxin and peroxiredoxins (Prdx) 1, 3, and 4 in the liver were down-regulated at 12 days post-HS compared to controls. The liver activity of thioredoxin reductase (TXNRD) and levels of peroxiredoxin1 (Prdx1) at 12 days post-HS were significantly decreased. The results reveal that there was a significant decrease in DNA methylation at 12 days post HS in liver tissues. In conclusion, pathway of thioredoxin system under HS may provide clues to nutritional strategies to mitigate the effect of HS in meat-type chicken.

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DNA Methylation of Seed in Response to Heat Stress in Brassica rapa L.

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2011.01597 ◽

2011 ◽

Vol 37 (9) ◽

pp. 1597-1604 ◽

Cited By ~ 1

Author(s):

Gui-Zhen GAO ◽

Fei YING ◽

Bi-Yun CHEN ◽

Hao LI ◽

Xiao-Dan LÜ ◽

...

Keyword(s):

Dna Methylation ◽

Heat Stress ◽

Brassica Rapa

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Secondary Functions of Arginine and Sulfur Amino Acids in Poultry Health: Review

Animals ◽

10.3390/ani10112106 ◽

2020 ◽

Vol 10 (11) ◽

pp. 2106

Author(s):

Fernanda Lima de Souza Castro ◽

Woo K. Kim

Keyword(s):

Amino Acids ◽

Heat Stress ◽

Bone Mineral ◽

Bone Development ◽

Sulfur Amino Acids ◽

Mineral Density ◽

Eimeria Spp ◽

Nutritional Strategies ◽

Eimeria Infection ◽

Health Review

Amino acids such as arginine, methionine, and cysteine are the precursors of essential molecules that regulate growth and health, being classified as functional amino acids. This review describes the metabolism of arginine and the sulfur amino acids and how they modulate, directly or indirectly, different tissues. Emphasis is placed on their effects in supporting health during challenging conditions, such as heat stress and Eimeria infection. The use of arginine has been shown to reduce abdominal fat pad in ducks and increase lean tissue and bone mineral density in broilers. Additionally, the sulfur amino acids have been shown to improve bone development and are beneficial during heat stress. The use of L-methionine increased the cortical and trabecular bone mineral densities, in laying hens. Moreover, the dietary inclusion of these amino acids could reduce the damage caused by Eimeria spp. infection by regulating the antioxidant system and cell repair. Understanding how these amino acids can mitigate stressful conditions may provide us novel insights of their use as nutritional strategies to modulate the health status of chickens.

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Antioxidant Activities and Selenogene Transcription in the European Sea Bass (Dicentrarchus labrax) Liver Depend, in a Non-linear Manner, on the Se/Hg Molar Ratio of the Feeds

Biological Trace Element Research ◽

10.1007/s12011-021-02835-7 ◽

2021 ◽

Author(s):

Marinelle Espino ◽

Harkaitz Eguiraun ◽

Oihane Diaz de Cerio ◽

José Antonio Carrero ◽

Nestor Etxebarria ◽

...

Keyword(s):

Antioxidant Activities ◽

Dicentrarchus Labrax ◽

Thioredoxin Reductase ◽

Sea Bass ◽

Molar Ratio ◽

Antioxidant Systems ◽

European Sea Bass ◽

Thioredoxin System ◽

Non Linear ◽

Linear Manner

AbstractFeeding 3.9 and 6.7 mg Hg/kg (Se/Hg molar ratios of 0.8 and 0.4, respectively) for 14 days negatively affected Dicentrarchus labrax growth and total DNTB- and thioredoxin-reductase (TrxR) activities and the transcription of four redox genes (txn1, gpx1, txnrd3, and txnrd2) in the liver, but a diet with 0.5 mg Hg/kg (Se/Hg molar ratio 6.6) slightly increased both reductase activities and the transcription of txn1, gpx1, and txnrd2. Feeding 6.7 mg Hg/kg for 53 days downregulated the genes of the thioredoxin system (txn1, txnrd3, and txnrd2) but upregulated gpx1, confirming the previously proposed complementarity among the antioxidant systems. Substitution of 20% of the feed by thawed white fish (hake) slightly counteracted the negative effects of Hg. The effects were not statistically significant and were dependent, in a non-linear manner, on the Se/Hg molar ratio of the feed but not on its Hg concentration. These results stress the need to consider the Se/Hg molar ratio of the feed/food when evaluating the toxicity of Hg.

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The Mechanism Underlying the Balance Between Heat Stress Resilience and Vulnerability are Mediated by Dna Methylation and De-Methylation at a Regulatory Segment of the Dna Encoding Corticotrophin-Releasing-Hormone

European Neuropsychopharmacology ◽

10.1016/j.euroneuro.2016.09.615 ◽

2017 ◽

Vol 27 ◽

pp. S508

Author(s):

Tomer Cramer ◽

Tali Rosenberg ◽

Tania Kisliouk ◽

Noam Meiri

Keyword(s):

Dna Methylation ◽

Heat Stress ◽

Dna Encoding ◽

Stress Resilience ◽

Releasing Hormone

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New Insights into the Potential of Endogenous Redox Systems in Wheat Bread Dough

Antioxidants ◽

10.3390/antiox7120190 ◽

2018 ◽

Vol 7 (12) ◽

pp. 190 ◽

Cited By ~ 2

Author(s):

Nicolas Navrot ◽

Rikke Buhl Holstborg ◽

Per Hägglund ◽

Inge Povlsen ◽

Birte Svensson

Keyword(s):

Nicotinamide Adenine Dinucleotide Phosphate ◽

Thioredoxin Reductase ◽

Heat Stability ◽

Wheat Bread ◽

Enzymatic System ◽

Redox Systems ◽

Bread Dough ◽

Seed Formation ◽

Protein Substrates ◽

Thioredoxin System

Various redox compounds are known to influence the structure of the gluten network in bread dough, and hence its strength. The cereal thioredoxin system (NTS), composed of nicotinamide adenine dinucleotide phosphate (NADPH)-dependent thioredoxin reductase (NTR) and thioredoxin (Trx), is a major reducing enzymatic system that is involved in seed formation and germination. NTS is a particularly interesting tool for food processing due to its heat stability and its broad range of protein substrates. We show here that barley NTS is capable of remodeling the gluten network and weakening bread dough. Furthermore, functional wheat Trx that is present in the dough can be recruited by the addition of recombinant barley NTR, resulting in dough weakening. These results confirm the potential of NTS, especially NTR, as a useful tool in baking for weakening strong doughs, or in flat product baking.

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Effect of heat stress on amino acid digestibility and transporters in meat-type chickens

Poultry Science ◽

10.3382/ps/pex027 ◽

2017 ◽

Vol 96 (7) ◽

pp. 2312-2319 ◽

Cited By ~ 21

Author(s):

W.S. Habashy ◽

M.C. Milfort ◽

K. Adomako ◽

Y.A. Attia ◽

R. Rekaya ◽

...

Keyword(s):

Heat Stress ◽

Amino Acid ◽

Meat Type

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Mapping the phenotypic repertoire of the cytoplasmic 2-Cys peroxiredoxin – thioredoxin system. 1. Understanding commonalities and differences among cell types

10.1101/224428 ◽

2017 ◽

Author(s):

Gianluca Selvaggio ◽

Pedro M. B. M. Coelho ◽

Armindo Salvador

Keyword(s):

Cell Lines ◽

Thioredoxin Reductase ◽

Cell Types ◽

Cell Physiology ◽

Related Factor ◽

Similar Response ◽

Redox Biology ◽

Analytical Approximations ◽

Thioredoxin System ◽

Reduction Capacity

AbstractThe system (PTTRS) formed by typical 2-Cys peroxiredoxins (Prx), thioredoxin (Trx), Trx reductase (TrxR), and sulfiredoxin (Srx) is central in antioxidant protection and redox signaling in the cytoplasm of eukaryotic cells. Understanding how the PTTRS integrates these functions requires tracing phenotypes to molecular properties, which is non-trivial. Here we analyze this problem based on a model that captures the PTTRS’ conserved features. We have mapped the conditions that generate each distinct response to H2O2 supply rates (νsup), and estimated the parameters for thirteen human cell types and for Saccharomyces cerevisiae. The resulting composition-to-phenotype map yielded the following experimentally testable predictions. The PTTRS permits many distinct responses including ultra-sensitivity and hysteresis. However, nearly all tumor cell lines showed a similar response characterized by limited Trx-S- depletion and a substantial but self-limited gradual accumulation of hyperoxidized Prx at high νsup. This similarity ensues from strong correlations between the TrxR, Srx and Prx activities over cell lines, which contribute to maintain the Prx-SS reduction capacity in slight excess over the maximal steady state Prx-SS production. In turn, in erythrocytes, hepatocytes and HepG2 cells high νsup depletes Trx-S- and oxidizes Prx mainly to Prx-SS. In all nucleated human cells the Prx-SS reduction capacity defined a threshold separating two different regimes. At sub-threshold νsup cytoplasmic H2O2 is determined by Prx, nM-range and spatially localized, whereas at supra-threshold νsup it is determined by much less active alternative sinks and μM-range throughout the cytoplasm. The yeast shows a distinct response where the Prx Tsa1 accumulates in sulfenate form at high νsup. This is mainly due to an exceptional stability of Tsa1’s sulfenate.The implications of these findings for thiol redox regulation and cell physiology are discussed. All estimates were thoroughly documented and provided, together with analytical approximations for system properties, as a resource for quantitative redox biology.AbbreviationsASK1, apoptosis signal-regulating kinase 1; Cat, catalase; GSH, glutathione; GPx1, glutathione peroxidase 1; Grx, glutaredoxin; KEAP1, Kelch-like ECH-associated protein 1; NRF2, nuclear factor erythroid 2-related factor 2; Prx, typical 2-Cys peroxiredoxin; PTTRS, peroxiredoxin / thioredoxin / thioredoxin reductase system; Srx, sulfiredoxin; Trx, thioredoxin; TrxR, thioredoxin reductase.

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Nutritional strategies to alleviate heat stress effects through feed restrictions and feed additives (vitamins and minerals) in broilers under summer conditions

Journal of Animal Behaviour and Biometeorology ◽

10.31893/2318-1265jabb.v7n3p123-131 ◽

2019 ◽

Vol 7 (3) ◽

pp. 123-131 ◽

Cited By ~ 1

Author(s):

Mohamed Abdelhameed Salah Abdelhameed ◽

◽

Lozovskiy Alexander Robertovich ◽

Ali Amany Muhammad Ahmed ◽

◽

...

Keyword(s):

Heat Stress ◽

Feed Additives ◽

Stress Effects ◽

Nutritional Strategies

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164 Genome-wide DNA Methylation Profiling in in Utero Heat-stressed Pigs as Measured by Whole-genome Bisulfite Sequencing

Journal of Animal Science ◽

10.1093/jas/skab235.157 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 87-88

Author(s):

Luiz F Brito ◽

Jacob M Maskal ◽

Shi-Yi Chen ◽

Hinayah R Oliveira ◽

Jason R Graham ◽

...

Keyword(s):

Dna Methylation ◽

Heat Stress ◽

Cellular Response ◽

Bisulfite Sequencing ◽

Cholesterol Biosynthesis ◽

In Utero ◽

Whole Genome ◽

Whole Genome Bisulfite Sequencing ◽

Differentially Methylated Genes ◽

Genome Bisulfite Sequencing

Abstract In utero heat stress (IUHS) has several postnatal consequences in pigs that compromise health, increase stress response, and reduce performance. These phenotypes may be caused by epigenetic modifications such as DNA methylation, which are heritable molecular modifications that impact gene expression and phenotypic outcomes without changing the DNA sequence. Therefore, we aimed to compare the DNA methylation profiles between in-utero thermoneutral (IUTN) and IUHS pigs to identify differentially methylated regions. Twenty-four pregnant gilts were evenly assigned to either a thermoneutral (17.5 ± 2.1°C) or heat stress (cycling 26 to 36°C) chamber from d 0 to 59 of gestation, followed by thermoneutral conditions (20.9 ± 2.3°C) for the rest of gestation and until the piglets were weaned. At 105 d of age, 10 IUTN and 10 IUHS piglets were euthanized and Longissimus dorsi muscle samples were collected and used to perform whole-genome bisulfite sequencing (WGBS). Purified genomic DNA was fragmented and bisulfite conversion was performed. Illumina platforms were used to sequence WGBS libraries. All pigs had similar proportions of methylation at CpG sites. Two-hundred-sixty-eight genomic regions were differentially methylated between IUTN and IUHS pigs. These identified regions are located across all pig chromosomes and ranged from 2 (SSC18) to 40 (SSC10). Eighty-five unique differentially-methylated genes were identified. These genes have been reported to be involved in key biological processes such as transcriptional repressor activity and tRNA processing (e.g., SKOR2,TRMT6, TSEN2), cellular response to heat stress (e.g.,CCAR2), placental vascularization (e.g.,FZD5), central nervous system (e.g.,VEPH1), cholesterol biosynthesis (e.g., CYB5R1), insulin receptor substrate (e.g.,IRS2), synaptic transmission (e.g.,RIMBP2), neurotrophic factor receptor activity (e.g.,LIFR), immune response (e.g., CD84), DNA repair (e.g., CHD1L), and cell proliferation and endocrine signaling (e.g., SSTR1, CYB5R1). These findings contribute to a better understanding of the epigenomic mechanisms underlying postnatal consequences of IUHS in pigs.

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