scholarly journals Application of Heating on the Antioxidant and Antibacterial Properties of Malaysian and Australian Stingless Bee Honey

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1365
Author(s):  
Nurul Ainaa Farhanah Mat Ramlan ◽  
Aina Syahirah Md Zin ◽  
Nur Fatihah Safari ◽  
Kim Wei Chan ◽  
Norhasnida Zawawi
Keyword(s):  
Heat Treatment ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Heat Treatments ◽  
Stingless Bee ◽  
Total Phenolic ◽  
Antioxidant Power

In the honey industry, heat treatments are usually applied to maintain honey’s quality and shelf life. Heat treatment is used to avoid crystallisation and allow the easy use of honey, but treatment with heat might affect the antioxidant and antibacterial activities, which are the immediate health effects of honey. This study will determine the effect of heat treatment on Malaysian and Australian stingless bee honey (SBH) produced by the common bee species in both countries. Eighteen honey samples were subjected to heat at 45 °C, 55 °C and 65 °C for one hour and subsequently analysed for their total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP) and minimum inhibitory concentration (MIC). The results show that all samples had high TPC, TFC and antioxidant activities before the treatment. The heat treatments did not affect (p < 0.05) the TPC, TFC and antioxidant activities in most samples, but did inhibit the antibacterial activities consistently in most of the samples, regardless of the bee species and country of origin. This study also confirms a strong correlation between TPC and TFC with FRAP activities for the non-heated and heated honey samples (p < 0.05). Other heat-sensitive bioactive compounds in SBH should be measured to control the antibacterial properties present.

Polyphenol Contents, Antioxidant and Antibacterial Activities of Aqueous Algerian Propolis Extracts

2021 ◽  
Author(s):  
E.K. Soltani ◽  
K. Zaim ◽  
K. Mokhnache ◽  
N. Haichour ◽  
S. Mezaache-Aichour ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Aqueous Extracts

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

scholarly journals Antioxidant Potential of Selected Korean Edible Plant Extracts

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yaejin Woo ◽  
Hyeji Lee ◽  
Yong-Seob Jeong ◽  
Ga Young Shin ◽  
Ja Gyeong Oh ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Total Phenolic ◽  
Edible Plant ◽  
Camellia Japonica ◽  
Antioxidant Power

This study aimed to evaluate the antioxidant activity of various plant extracts. A total of 94 kinds of edible plant extracts obtained from the Korea Plant Extract Bank were screened for cytotoxicity, following which the total phenolic content of 24 shortlisted extracts was determined. Of these, extracts from three plants, namely, Castanea crenata (CC) leaf, Camellia japonica (CJ) fruit, and Viburnum dilatatum (VD) leaf, were examined for antioxidant capabilities by measuring radical scavenging activity, ferric reducing/antioxidant power, and lipid peroxidation inhibitory activity. In addition, cellular antioxidant activities of the three extracts were assessed by a cell-based dichlorofluorescein assay and antioxidant response element (ARE) reporter activity assay. The results demonstrated that all three extracts concentration-dependently scavenged free radicals, inhibited lipid peroxidation, reduced the cellular level of reactive oxygen species, and increased ARE-luciferase activity, indicating antioxidant enzyme-inducing potential. In particular, CJ extract showed significantly greater antioxidative activity and antimigratory effect in a breast cancer cell line compared to CC and VD extracts. Hence, CJ extract deserves further study for its in vivo functionality or biologically active constituents.

scholarly journals Antioxidant activity and polyphenol composition of Pistacia terebinthus fruit from Tessala (Western Algeria)

2021 ◽  
Vol 5 (11) ◽  
pp. 30-34
Author(s):  
Nazim Bellifa ◽  
Abdelhak Ismail Benhaddou ◽  
Houssem Eddine Ferkous ◽  
Mohammed Adil Selka ◽  
Houari Toumi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Scavenging Effect ◽  
Coffee Substitute ◽  
Ferric Reducing Antioxidant Power ◽  
Pistacia Terebinthus

Background: Consumption of traditional herbal beverages has been generally increased in the last decades, Terebinth coffee, known as ‘‘menengic coffee’’ in Turkish, is one of the most consumed herbal coffees in Turkey, turpentine tree is one of the components of the Mediterranean bush, particularly in Algeria, known as Betoum el Kiffan is largely used as food and in traditional medicine. Aims: In this study, Total phenol, flavonoid content, and antioxidant activity of three extracts of Pistacia trebinthus fruit growing in Algeria was measured using radical scavenging activity tests and metal-related tests including, ferric-reducing antioxidant power (FRAP). The chemical composition profile of the fruits and the coffee brands was identified by thin-layer chromatography, the effects of roasting method of this fruit was rivaled also. Materials and Methods: The total phenolic content of the extracts was determined using the Folin-Ciocalteu method. All extracts of the terebinth fruits and coffee brands displayed a high DPPH scavenging effect. Results: The results of the ferric-reducing antioxidant power show that the reduction capacity is proportional to the increase in the concentration of the samples. All the extracts of the plant exhibit antioxidant activities lower than those of the reference product besides the infusion extract of the P. terebinthus roasted coffee, which is the most active with an optical density of 1.68 nm at a concentration of 400 μg/mL. The chromatography results show that the various extracts of Pistacia terebinthus fruit carry a large number of polyphenols, in particular the carboxylic acids phenols. Conclusions: The plant can be considered as a coffee substitute and opens up promising avenues for the food and pharmaceutical industry in Algeria. Keywords: Antioxidant, Pistacia terebinthus, Coffee, FRAP, polyphenol.

Investigation of Active Components and Antioxidant Activities in Peel and Pulp Extracts of Citrus Reticulata Blanco cv. Suavissima Fruit

2011 ◽  
Vol 343-344 ◽  
pp. 1098-1102 ◽  
Author(s):  
Xiang Tao Chen ◽  
Dong Qing Jiang ◽  
Fang Fang Chen ◽  
Ke Yuan
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Citrus Reticulata ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Citrus Reticulata Blanco

Citrus. reticulata Blanco cv. Suavissima friut has long been regarded as a food and medicinal plant. We investigated the antioxidant activity of the ethanol extract of peel and pulp part of fruit growned in Zhejiang Province, PR. China by several in-vitro systems of assay, namely DPPH radical-scavenging activity, ABTS.+ radical-scavenging activity, ferric reducing antioxidant power (FRAP) assay. Total phenolic content was measured by Folin-Ciocalteu reagent. The total flavonoids contents were measured using a modified colorimetric method.The extracts of different samples were found to have different levels of antioxidant activity in the systems tested. Ethanol extracts of the fruit showed stronger antioxidant activities compared with those of the commercial compound (Trolox). The results suggest that Citrus. reticulata Blanco cv. Suavissima friut can be a good source of natural antioxidant.

scholarly journals Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4589
Author(s):  
Soumaya Touzani ◽  
Hamada Imtara ◽  
Shankar Katekhaye ◽  
Hamza Mechchate ◽  
Hayat Ouassou ◽  
...  
Keyword(s):  
Cinnamic Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Photodiode Array Detection

The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.

scholarly journals Effectiveness of Solvent Extraction on Phytochemicals and Antioxidant Activities from Fresh and Dried Wheatgrass

2021 ◽  
pp. 1-10
Author(s):  
Towkir Ahmed Ove ◽  
Anwara Akter Khatun ◽  
Said Bin Saifullah ◽  
Maruf Ahmed
Keyword(s):  
Solvent Extraction ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Chlorophyll Contents ◽  
Methanolic Extracts ◽  
Ferric Reducing Antioxidant Power

Aims: This study was intended to investigate and quantify the phytochemicals and the antioxidant activities of fresh and powdered wheatgrass. Study Design:  The experiment was performed by solvent extraction technique for determining the phytochemicals of wheatgrass samples. Place and Duration of Study: The study was conducted at the Food engineering laboratory in Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh between June 2018 and December 2018. Methodology: Fresh and powdered wheatgrass were used for the experiment. Methanol and ethanol solutions were separately utilized, in an attempt to assume the efficient solvent for extraction. UV-Vis spectrophotometric methods were employed in general throughout the study. Total phenolic contents (TPC), total flavonoid contents (TFC), DPPH scavenging activity, ferric reducing antioxidant power (FRAP) and the total chlorophyll contents of both solvent extracts were analyzed. Results: Phytochemicals and antioxidant activity were found lower in both solvent extracts of powdered wheatgrass than fresh leaves. Phenolic content and Chlorophyll were significantly (P < 0.05) higher in fresh samples (extracts). Due to heating, a significant decrease was observed in the DPPH radical scavenging activity of powdered extracts. Ferric Reducing Antioxidant Power (FRAP) was significantly (P < 0.05) higher in methanolic extracts than in ethanolic extracts. No significant (P > 0.05) difference was observed in total flavonoids contents. Conclusion: These findings endorsed that both solvents demonstrate competitive potentials to extract bioactive compounds from wheatgrass and its derivatives and hence can be chosen specifically according to the components.

scholarly journals Phytochemical Screening, Antioxidant and Antibacterial Activities of Pollen Extracts from Micromeria fruticosa, Achillea fragrantissima, and Phoenix dactylifera

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 676
Author(s):  
Omar Sadeq ◽  
Hamza Mechchate ◽  
Imane Es-safi ◽  
Mohamed Bouhrim ◽  
Fatima zahra Jawhari ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Male Flower ◽  
Phoenix Dactylifera ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Achillea Fragrantissima ◽  
Micromeria Fruticosa

Pollen is a male flower gametophyte located in the anthers of stamens in angiosperms and a considerable source of compounds with health protective potential. In the present work, phytochemical screening was carried out as well as analysis of the antioxidant and antibacterial properties of pollen extracts from Micromeria fruticosa, Achillea fragrantissima, and Phoenix dactylifera growing wild in Palestine. Phytochemical screening examined the total flavonol, flavone and phenolic content. The DPPH (1,2-Diphenyl-1-Picrylhydrazyl) and FRAP (ferric reducing antioxidant power) methods were used to assess antioxidant propriety, and disc diffusion, minimum inhibitory and bactericidal concentration tests were used to test the pollen extract’s antibacterial activity against multidrug-resistant (MDR) clinical isolates. The highest level of total phenolic was found in the extract of Micromeria fruticosa (56.78 ± 0.49 mg GAE (Gallic Acid Equivalent)/g). The flavone and flavonol content of samples ranged from 2.48 ± 0.05 to 8.03 ± 0.01 mg QE (Quercetin Equivalent)/g. Micromeria fruticosa pollen with IC50 values of 0.047 and 0.039 mg/mL in the DPPH and FRAP assays, respectively, showed the greatest radical scavenging action. In addition, this pollen showed a mild antibacterial action against the microorganisms studied, with MICs varying from 0.625 to 10 mg/mL and inhibition diameters ranging from 13.66 ± 1.5 to 16.33 ± 1.5 mm.

Evaluation of Free Radical Scavenging Activity and Reducing Power of Careya arborea Roxb.

2019 ◽  
Vol 886 ◽  
pp. 86-91
Author(s):  
Wanvisa Ninlaor ◽  
Thaksaorn Thongom ◽  
Chawapon Phoomphong ◽  
Warachate Khobjai
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Active Substances

Antioxidant plays an important role in inhibiting and scavenging radicals, thus providing protection for human against infection and degenerative diseases. The aim of this study was to evaluate the effect of the solvent on the extraction of the antioxidant compounds from Careya arborea (CA) Roxb. The leaves of CA were extracted by using water, ethanol, methanol, and chloroform. The total phenolic content of the extracts was determined by the Folin-Ciocalteu reagent and their antioxidant activity was measured using 2, 2-diphenyl-1 picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. The antioxidant activities of the extracts for DPPH and FRAP were in the order: water > methanol > ethanol > chloroform extract. According to the results, water is the most suitable solvent for extraction of active substances with antioxidant activities.

Optimization of Maclura cochinchinensis Extract as a Cosmeceutical Component for Antioxidant and Anti-Tyrosinase Activities

2020 ◽  
Vol 859 ◽  
pp. 188-193
Author(s):  
Boonyadist Vongsak ◽  
Sunan Jaisamut ◽  
Kanokporn Gonsap ◽  
Porntip Parmontree
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Ultrasound Technique ◽  
Antioxidant Power ◽  
Chronic Fever

Maclura cochinchinensis Corner or Kae Lae in Thai belongs to the Moraceae family. The plant is found in Southeast Asian countries and used in traditional medicine as a tonic agent to treat chronic fever and skin infections. Previous studies have demonstrated that plants in Moraceae family, such as Morus alba and Artocarpus lakoocha are rich source of antioxidant and anti-tyrosinase agents. Thus, the current study aimed at comparing different solvent extracts (50% ethanol, 80% ethanol, methanol by ultrasound and water decoction) of M. cochinchinensis heartwood for their antioxidant and anti-tyrosinase activities. Total phenolic content, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and anti-lipid peroxidation assays were performed to compare the antioxidant activity, whereas, Dopachrome method was performed for anti-tyrosinase activity. The results demonstrated that 80% ethanol and methanol extracts had better antioxidant activities based on EC50 value of DPPH (9.96±0.21 and 9.50±0.46 μg/mL, respectively), ABTS (6.54±0.13 and 6.58±0.69 μg/mL, respectively), anti-lipid peroxidation (228.31±2.03 and 231.43±1.41 μg/mL, respectively), and anti-tyrosinase (7.60±0.29 and 7.91±0.41 μg/mL, respectively) assays. Further, 80% ethanol extract was selected to evaluate the bioactive compound using bioactivity-guided isolation. Oxyresveratrol identified as bioactive compound, exhibited higher activities than the positive controls (quercetin and kojic acid). In conclusion, 80% ethanolic M. cochinchinensis heartwood extract obtained using the ultrasound technique exhibited strong antioxidant and anti-tyrosinase effects and can be used as a potential active ingredient for cosmeceutical products. Oxyresveratrol could be used as bioactive marker for quality control of the extract and products.

scholarly journals Antimicrobial and Antioxidant Activities of a Malaysian Medicinal Plant Anisophyllea Disticha (Jack) Baill. And Quantification of Its Phenolic Constituents

2021 ◽  
Vol 50 (3) ◽  
pp. 515-521
Author(s):  
Nurul Ashikin Abdul Bari ◽  
Sahena Ferdosh ◽  
Md Zaidul Islam Sarker
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
High Positive Correlation ◽  
Antioxidant Power ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Phenolic Constituents ◽  
Ferric Reducing Antioxidant Power

The ethanolic extracts of different parts of Anisophyllea disticha were tested for antimicrobial capability using disc diffusion assay. The result revealed positive growth inhibitory activity, but was considered weak for all extracts against Gram positive bacteria. Leaf extract displayed the highest zone of inhibition with values of 10.67 ± 0.58 and 8.67 ± 0.58 mm against Staphylococcus epidermidis and S. aureus, respectively. On the other hand, stem showed the highest amount of total phenolic content (TPC) (27.73 ± 4.10 mg GAE/g extract) and similar trend was also observed in antioxidant potential in terms of DPPH radical scavenging activity (66.20 ± 11.74%) and ferric reducing antioxidant power assay (FRAP) (203.63 ± 16.03 mg Fe(II)/g extract). High positive correlation was found between TPC and antioxidant activities, suggesting that phenolic compounds contribute to the antioxidant power of plant extracts. HPLC analysis identified and quantified gallic acid, p-coumaric, ferulic acid and quercetin in the extracts. Bangladesh J. Bot. 50(3): 515-521, 2021 (September)

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