scholarly journals Industry-Friendly Hydroethanolic Extraction Protocols for Grateloupia turuturu UV-Shielding and Antioxidant Compounds

2020 ◽  
Vol 10 (15) ◽  
pp. 5304
Author(s):  
Rafael Félix ◽  
Ana M. Carmona ◽  
Carina Félix ◽  
Sara C. Novais ◽  
Marco F. L. Lemos
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Procedure ◽  
Extraction Methods ◽  
Antioxidant Compounds ◽  
Uv Absorbance ◽  
Grateloupia Turuturu ◽  
Solid Ratio ◽  
Uv Shielding ◽  
Cosmetic Industry

Grateloupia turuturu is an invasive macroalga on the Iberian coast, known to produce bioactive compounds with different cosmeceutical bioactivities, namely UV shielding and antioxidants. The goal of this study was to optimize the extraction procedure of main bioactivities of this species with cosmetic potential, using Response Surface Methodology. Two Box–Behnken designs were used to evaluate the effect of ethanol concentration (0–50%), liquid-solid ratio, time, pH, and temperature on yield, UV absorbance, and antioxidant activity. Both optimizations showed a similar trend: aqueous extracts have higher yields and extracts performed with ethanol as part of the solvent have higher activities concerning UV absorbance and antioxidant activity. For all the extracts an absorption peak between 320 and 340 nm was observed. This data now allows further studies by narrowing the extracts worthful of characterization. The development of industry-friendly extraction methods allows the valorization of this invasive species, contributing for the potential creation of natural and eco-friendly products by the cosmetic industry while contributing to the restoration of affected environments.

Comparison of Two Protein Extraction Methods for Tea Residues

2011 ◽  
Vol 138-139 ◽  
pp. 933-936 ◽  
Author(s):  
Xuan Chen ◽  
Hong Yu Luo ◽  
Jun Yu ◽  
Peng Xiang Yue ◽  
Lin Zhou ◽  
...  
Keyword(s):  
High Temperature ◽  
Protein Extraction ◽  
Ethanol Concentration ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Factorial Experiments ◽  
Extraction Technology ◽  
Digestion Method ◽  
Solid Ratio ◽  
Lower Temperature

Alcohol-alkali method and base digestion method were investigated to extract proteins from tea residues, respectively. According to single factorial experiments, results showed that the optimal extraction technology of alcohol-alkali method were pH 12, temperature of 80 °C, ethanol concentration of 60%, liquid-solid ratio of 40, 60 min, and the protein extraction rate reached 15.0%. And the optimal extract conditions of base digestion were pH 12, temperature of 80 °C, liquid-solid ratio of 50, 80 min, which made the protein yield reached 31.5%. Furthermore, alcohol-alkali method was more beneficial to protein extraction from tea residues under lower temperature and weak alkali condition (40-60 °C, pH 8-10). While base digestion had higher extraction yield under high temperature and strong alkali condition (60-80 °C, pH 11-12).

Optimizing extraction of berberine and antioxidant compounds from barberry by maceration and pulsed electric field-assisted methods

2020 ◽  
pp. 1-16 ◽  
Author(s):  
Mozhdeh Sarraf ◽  
Adel Beig-babaei ◽  
Sara Naji-Tabasi
Keyword(s):  
Antioxidant Activity ◽  
Optimum Condition ◽  
Electric Field ◽  
Ethanol Concentration ◽  
Pulsed Electric Field ◽  
Antioxidant Compounds ◽  
Good Source ◽  
Valuable Compounds ◽  
Central Composite ◽  
Different Parts

BACKGROUND: Barberry is a valuable plant, which is useful in the treatment of different diseases. There are valuable compounds in different parts of barberry that are pruned per year and discarded as waste. OBJECTIVE: In this study, the amount of berberine, phenolic compounds and antioxidant activity of barberry fruits, leaves, and stems of different barberry species (B.integerrima and B.thunbergii) were investigated. METHODS: Central composite design of response surface methodology (RSM) were used in this study to investigate the extraction variables (time: 2–24 h, temperature: 24–70°C and ethanol concentration: 50–90%) in the extraction of berberine from the stem of B.integrrima. The responses used as criteria were the amount of berberine, scavenging radical DPPH, and polyphenol. Also, pulsed electric field-assisted (PEF) was applied as a pretreatment (Pulse strengths of 250, 1000 and 1250 Vcm–1; Pulse numbers (n) of 50 and 100; frequency (f) of 1 Hz) and then the extraction was conducted in optimum condition. PEF increased significantly the amount of berberine. RESULTS: The stem of B.integrrima had the most amounts of antioxidant activity and berberine content, and it was selected for more investigation. The optimum condition in the maceration method was: 90% of ethanol, 70°C, and 3.36 h (141.6 min). According to the result of PEF method, the best condition for extraction of berberine from barberry stems was 1000 Vcm–1 and n = 100. CONCLUSIONS: The results demonstrated that the extract of B.integrrima stem is a good source of berberine, antioxidant, and has the potential to be applied in various industries.

scholarly journals Optimization of the extraction of antioxidant compounds from quinoa (Chenopodium quinoa Willd.)

2020 ◽  
Vol 38 (3) ◽  
pp. 436-441
Author(s):  
Julia Luisetti ◽  
Héctor Lucero ◽  
María Cristina Ciappini
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Ethanol Concentration ◽  
Chenopodium Quinoa ◽  
Antioxidant Compounds ◽  
Drying Temperature ◽  
Extraction Solvent ◽  
Solid Ratio ◽  
Influential Variable ◽  
Natural Foods

Argentina contains a great biodiversity of natural foods such as quinoa that can be included in the human diet because of their nutritional characteristics and content of bioactive compounds. Among other properties, these bioactive have an antioxidant capacity that protects biomolecules from oxidant damage. Bioactive compounds contribute beneficially to diverse antimicrobial, anti-inflammatory and anti-carcinogenic physiological activities. The objective of this study was to optimize the parameters for the extraction of antioxidant compounds from quinoa: drying temperature of the grain, liquid/solid ratio (L/S), and ethanol concentration in the extraction solvent, based on an experimental design of three variables at three levels. A face-centered central composite design was used. The proposed levels were 40°C, 60°C and 80°C; 20:1, 30:1 and 40:1, and 30%, 50%, and 70% v/v of ethanol. Antioxidant capacity was determined by the capture of the DPPH free radical. The values obtained were from 16.3 mg to 161.5 mg of equivalent trolox (ET) 100 g-1 of quinoa. The maximum antioxidant capacity was obtained for the L/S ratio of 28:1, and the drying temperature of the grain was 58°C and 39% v/v of ethanol in the extraction solvent. The ethanol concentration was the most influential variable in the antioxidant compound extraction.

Optimization and Comparison of Three Methods on Anthocyanins Extraction from Blackcurrant (Ribes nigrum L.) Using RSM

2011 ◽  
Vol 361-363 ◽  
pp. 691-700 ◽  
Author(s):  
Yu Yang ◽  
Sheng Xin Zhao ◽  
Ya Qin Xu ◽  
Ze Yuan Yu
Keyword(s):  
Ethanol Concentration ◽  
Extraction Methods ◽  
Perfect Agreement ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Natural Colorants ◽  
Ultrasonic Assisted ◽  
Optimal Values ◽  
Commercial Applications ◽  
Solid Liquid

Response surface methodology (RSM) complemented with a central composite design (CCD) was employed to optimize and compare three different anthocyanins extraction methods (solid–liquid extraction(SLE), ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE)) from blackcurrant fruits. The aim was to obtain extracts with high anthocyanins content, which would be potentially interesting for commercial applications as natural colorants. Three major independent variables such as extraction time(min), ethanol concentration (%,v/v), solution to solid ratio (mL/g) were coded at five levels and their actual values were selected on the basis of preliminary experimental results. Results indicated that the yields of anthocyanins varied when extracted with different method, the order of anthocyanins yield from high to low was: MAE (95.77%), UAE (93.65%) and SLE (90.82%). Among three reaction parameters, ethanol concentration consistently tended to significantly affect the anthocyanins yield for above three models (p < 0.0001). MAE was the best of three methods in this study and the optimal values were as follows: extract time controlled at 6.3 min, ethanol concentration maintained at 72.8%, solution to solid ratio equaled to 8.1:1 and working power at 400W. The experimental anthocyanins value under above optimum conditions could reach to 95.77±1.05% that was in perfect agreement with the predicted model 95.52%.

scholarly journals Reflux Extraction Optimization and Antioxidant Activity of Phenolic Compounds from Pleioblastus amarus (Keng) Shell

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 362
Author(s):  
Yuan Ma ◽  
Ailian Meng ◽  
Ping Liu ◽  
Yuanyuan Chen ◽  
Anqi Yuan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Composition Analysis ◽  
Extraction Temperature ◽  
Factors Affecting ◽  
Solid Ratio ◽  
Ic50 Values ◽  
Phenol Composition

Phenols were extracted from the Pleioblastus amarus (Keng) shell (PAS) using ethanol. A Plackett–Burman assessment indicated that the factors affecting polyphenol extraction included the ethanol concentration, extraction temperature, liquid to solid ratio, extraction time, and reflux extraction times; the best extraction parameters were the ethanol concentration of 75%, a 20:1 liquid to solid ratio, and an extraction time of 2.1 h. The number of polyphenols was 7.216 mg/g. Furthermore, the phenol composition analysis showed the presence of p-Coumaric acid (196.88 mg /mL) and rutin (312.9 mg /mL), which were used for the in vitro extraction and determination of the antioxidant activity. According to the A, B, C, and D antioxidant activity assays, the ethyl acetate phase was the strongest with low IC50 values of 0.169 ± 0.01 mg/mL, 0.289 ± 0.01 mg/mL, 0.372 ± 0.01 mg/mL, and 1.029 ± 0.03 mg/mL, respectively, confirming high antioxidant activity. For the n-butanol and petroleum ether phases, antioxidant activity was lower. This study showed that the polyphenol extract from Pleioblastus amarus (Keng) shell displayed excellent antioxidant activity, enhancing its practical application.

scholarly journals UPLC-ESI-MS/MS Analysis and Evaluation of Antioxidant Activity of Total Flavonoid Extract from Paeonia lactiflora Seed Peel and Optimization by Response Surface Methodology (RSM)

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Guiqin Shi ◽  
Jiaxin Shen ◽  
Tao Wei ◽  
Fei Ren ◽  
Famou Guo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Paeonia Lactiflora ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasound Assisted

In this study, the ultrasound-assisted extraction (UAE) of flavonoid from Paeonia lactiflora seed peel was optimized by response surface methodology (RSM). Single-factor experiments and a three-factor three-level Box-Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction: ethanol concentration ( X 1 ), liquid-solid ratio ( X 2 ), and ultrasonic time ( X 3 ). The results showed that the optimal flavonoid yield (10.9045 mg RE/g) was as follows: ethanol concentration 62.93%, ultrasonic time 64.56 min, and liquid-solid ratio 24.86 mL/g. The optimized extract of P. lactiflora seed shell was further analyzed by UPLC-ESI-MS/MS, and 20 main flavonoids were identified and quantified, among which protocatechuic acid, vanillic acid, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzaldehyde had the highest content. Furthermore, the results of the antioxidant test showed that the P. lactiflora seed peel extract obtained under optimized UAE conditions exhibited good antioxidant activity. The experimental results showed that ultrasound-assisted extraction was a fast, efficient, and simple method for extracting active ingredients from P. lactiflora seed peel, thereby making this byproduct a promising source of compounds in food and healthcare sectors.

scholarly journals Optimisation of ultrasound assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka (Leptospermum scoparium) for use as a phytomedicine against Alzheimer’s disease

2020 ◽  
Vol 50 ◽  
Author(s):  
Hafiz Majid ◽  
Filipa V. M. Silva
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Antioxidant Compounds ◽  
Ache Inhibition ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Background: Alzheimer’s disease is a progressive mental deterioration related to ageing and senility. Approved drugs that inhibit acetylcholinesterase (AChE) enzyme activity in the human brain are one of the ways to control the natural progression of this disease. The present study reports on the optimisation of ultrasound-assisted extraction of antiacetylcholinesterase and antioxidant compounds from manuka leaves using response surface methodology. Methods: A Box-Behnken design was used to investigate the effect of extraction temperature (40–60°C), time (1–20 min), and ethanol concentration (30–70%) on AChE inhibition, antioxidant activity, and extraction yield. Results: The values of AChE, radical scavenging activity (RSA) and yield predicted by the models generated were similar to the experimental values. Extraction time, ethanol concentration and temperature were significant in all the responses. Optimum extraction conditions for maximum AChE inhibition (74%), RSA (79%) and yield (50%) were successfully validated experimentally and the IC50 of the optimised extracts were reduced to 28.5 (from 66.0) and 2.37 (from 32.4) ?g/ mL for AChE and antioxidant activity, respectively. The optimisation enabled an increase in the extraction yield from 21% to 49%. Conclusions: In view of the significant bioactive properties determined, with possible beneficial effects on memory deficit, we would encourage the use of the manuka leaf extract for the development of new phytopharmaceuticals to improve brain function and control dementias such as Alzheimer’s disease. One other application could be as a beverage for the preparation of tea infusions.

scholarly journals Natural Antioxidant Activity and Compounds Content from Wastes of Euterpe edulis Berries

2017 ◽  
Vol 9 (3) ◽  
pp. 178 ◽  
Author(s):  
Beatriz Da Silva Frasao ◽  
Marion Pereira da Costa ◽  
Bruna Leal Rodrigues ◽  
Bruno Reis Costa Lima ◽  
Carlos Adam Conte-Junior
Keyword(s):  
Antioxidant Activity ◽  
Microwave Power ◽  
Aqueous Ethanol ◽  
Ethanol Concentration ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Euterpe Edulis ◽  
Monomeric Anthocyanins ◽  
Exposition Time

The Euterpe edulis (Juçara) is native to Brazil, which berries and wastes present high antioxidant content. Therefore, in this study, microwave-assisted extraction (MAE) was investigated for antioxidant compounds extraction from E. edulis waste and maximized antioxidant activities using response surface methodology coupled with a central composite design. Three factors were observed: microwave power (400/500/600 W), exposition time (30/60/90 sec) and ethanol concentration solvent (40/60/80%). The extracts were characterized by determination of total phenolic (TPC), flavonoids (TFC), monomeric anthocyanins (TAC), tannins content (TTC), and in vitro antioxidant assay (AA%). The yield of TPC, TFC, TAC, and TTC varied at 595.43-2171.34 mg GAE.100 g DM-1, 137.36-251.24 mg QE.100 g DM-1, 179.32-354.38 mg C-3-GE.100 g DM-1 and 0.23-1.00 µg TAE.100 g DM-1, respectively. The optimal MAE parameters for TPC was microwave power 668.18 W, exposition time 110.45 s and aqueous ethanol concentration 93.64%, for TFC same parameters observed; though for TAC the different parameters were 532.28 W, and for TTC 9.55 s. However, for antioxidant activity, the parameters were 668.18 W, 110.45 s time and 64.41% of aqueous ethanol solvent. Therefore, this methodology was successfully applied for optimal extraction of total phenolics, flavonoids, monomeric anthocyanins and tannins from juçara waste and obtain optimal antioxidant activity.

scholarly journals Evaluation of the Antioxidant Activity of Coreopsis Tinctoria Nuff. and Optimisation of Isolation by Response Surface Methodology

2014 ◽  
Vol 64 (3) ◽  
pp. 369-378 ◽  
Author(s):  
Shumin Lan ◽  
Jingming Lin ◽  
Ni Zheng
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Hydroxyl Radical ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Radical Scavenging ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Coreopsis Tinctoria ◽  
Assisted Extraction

Abstract The aim of the present study was to determine the antioxidant activity of Coreopsis tinctoria flowering tops (CTFT). Studies were conducted to obtain suitable extraction conditions for chlorogenic acid, quercetin, luteolin, apigenin and kaempferol, which were identified and quantified by HPLC. Response surface methodology was employed to optimise the ultrasound-assisted extraction conditions including extraction time, ethanol concentration and liquid-solid ratio. The antioxidant activity of the extracts was analysed using various antioxidant models, such as DPPH, ABTS and hydroxyl radical scavenging assay. CTFT extracted for 15.0 min with ethanol at a concentration of 60.4 % and with liquidsolid ratio 27.5:1 possessed a considerable amounts of total flavonoids and polyphenols (18.9 %). This extract showed higher scavenging activity of ABTS and hydroxyl radical activity than rutin, however not in the DPPH test. We may assume that CTFT possess antioxidant and free radical scavenging potentials.

Comparison of Bioactive Compounds of Matricaria recutita Extracted by Ultrasound and Maceration and their Effects on Preventing Sunflower Oil During Frying

2019 ◽  
Vol 15 (2) ◽  
pp. 156-164 ◽  
Author(s):  
Reza Farahmandfar ◽  
Maryam Asnaashari ◽  
Yegane Asadi ◽  
Batool Beyranvand
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Bioactive Compounds ◽  
Sunflower Oil ◽  
Radical Scavenging ◽  
Stability Index ◽  
Acid Value ◽  
Extraction Methods ◽  
Oil Stability Index ◽  
Matricaria Recutita

Background: It is important to study about the use of natural antioxidants as alternatives to synthetic ones due to the possibility of carcinogenic effects of synthetic antioxidants. This study is comparing the effect of the ultrasound-assisted and maceration extraction methods on antioxidant activity of Matricaria recutita. Methods: Bioactive compounds including phenolic, tocopherol, flavonoid and tannins and antioxidant activity of the extracts were evaluated. Moreover, extracts obtained from ultrasound and maceration methods were added to sunflower oil without any antioxidants at level of 200, 500 and 800 ppm, after that samples were heated at 180°C. Oxidation of the samples were evaluated after 0, 4, 8, 12, 16, 20 and 24 hours by measuring Peroxide Value (PV), Conjugated Diene (CD), Iodine Value (IV), Carbonyl Value (CV), Total Polar Compounds (TPC), Oil Stability Index (OSI), Color Index (CI) and acid value (AV). Results: The result showed total phenol (42.90 mg gallic acid/g extract), tocopherol (120.46 &#181;g &#945; - tocopherol/ml extract), flavonoid (2.64 mg/100 g extract) and tannins (3.89 mg gallic acid/g extract) of ultrasound extracts were higher than maceration extracts. Antioxidant activity of the extract was evaluated by DPPH assay which indicated 800 ppm of the Matricaria recutita extracted by ultrasound was the highest radical scavenging ability. Conclusion: Result indicated both ultrasound and maceration extracts could increase the oil oxidative stability but could not increase compared to BHA. In most cases, the extract samples by ultrasound had a better effect on stabilizing of sunflower oil during frying.

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