scholarly journals Thymus vulgaris Essential Oil and Hydro-Alcoholic Solutions to Counteract Wooden Artwork Microbial Colonization

2021 ◽  
Vol 11 (18) ◽  
pp. 8704
Author(s):  
Silvia Sparacello ◽  
Giuseppe Gallo ◽  
Teresa Faddetta ◽  
Bartolomeo Megna ◽  
Giovanna Nicotra ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antimicrobial Activities ◽  
Alcoholic Solution ◽  
Microbial Colonization ◽  
Supplementary Information ◽  
Thymus Vulgaris ◽  
Sabouraud Agar ◽  
Agar Disc ◽  
Pharmaceutical Industries

Aromatic plants represent a source of natural products with medicinal properties, and are also utilized in the food and pharmaceutical industries. Recently, the need for eco-compatible and non-toxic products, safe for both the environment and human health, have been proposed for the sustainable conservation of historic–artistic artifacts. In this study, in order to counteract microbial colonization (Aspergillus sp., Streptomyces sp., Micrococcus sp.) on wooden artwork surfaces, Thymus vulgaris L. (Lamiaceae) essential oil (EO) and hydro-alcoholic (HA) solutions were applied in a polyphasic approach. The antimicrobial activities of EO and HA solutions were preliminarily assessed by agar disc diffusion (ADD) and well plate diffusion (WPD) in vitro methods, defining the specific concentration useful for bacterial and fungal genera, identified by optical microscopies, in vitro cultures (nutrient or Sabouraud agar), and DNA base molecular biology investigations. Specifically, the microbial patina was directly removed by a hydro-alcoholic solution (while evaluating the potential colorimetric change of the artwork’s surface) combined with exposure to EO volatile compounds, performed in a dedicated “clean chamber”. This study proposes, for the first time, the combined use of two plant extracts to counteract microbial development on wooden artworks, providing supplementary information on these products as bio-agents.

scholarly journals Chemical Characterization and In vitro Antimicrobial Activity of Caralluma europaea Essential Oil and Its Synergistic Potential with Conventional Antibiotics

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Loubna Ait Dra ◽  
Abdellah Aghraz ◽  
Brahim Boualy ◽  
Saadia Oubaassine ◽  
Mustapha Barakate ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Aerial Part ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Acid Oxidation ◽  
Pharmaceutical Industries

Aim: This study was carried out to investigate the antioxidant and antimicrobial activities of essential oil from the aerial part of Caralluma europaea and to evaluate the synergistic potential between essential oil and antibiotics. Methodology: The chemical composition, antioxidant, antimicrobial activities and synergetic interaction between antimicrobial agents and essential oil isolated by hydrodistillation from the aerial part of C. europaea were evaluated. The chemical composition was analyzed by a Gas chromatography/mass spectrometry (GC/MS) system. Antioxidant activity was measured employing three methods: scavenging of free radical DPPH, reducing power assay and the inhibition of linoleic acid oxidation. The antimicrobial activity of essential oil against microbial strains was qualitatively and quantitatively assessed by the presence or absence of inhibition zones diameters, and MIC values. The in vitro association between essential oil and some commercial antibiotics was also investigated. Results: The GC/MS analysis shows that a total of 21 constituents were identified and the main compounds were Terpinolene (19.5%), α-Terpinene (16.2%) and Linalool (15.3%). Antioxidant study showed that essential oil exhibited antioxidant activity with IC50 values ranging from 0.32 mg/ml to 1.45 mg/ml. The results of antimicrobial activity showed that the essential oil had an inhibitory effect against the majority of tested microorganisms except K. pneumonia and  P. aeruginosa. Gram-positive bacteria were found to be more sensitive than Gram-negative ones. Furthermore, essential oil approved an interesting antifungal activity against yeast species. Out of 25 combinations tested 64% showed total synergism, 20% had a partial synergistic interaction and 16% showed no effect. The best synergistic effect was obtained with the combination essential oil-gentamycin. Conclusion: Our results are of a great importance and suggest that C. europaea essential oil contain bioactive compounds with antioxidant and antimicrobial properties with possible applications in the food and pharmaceutical industries.

scholarly journals EXTRACTION, ISOLATION, AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS AND ESSENTIAL OIL FROM SYZYGIUM JAMBOS

2017 ◽  
Vol 10 (8) ◽  
pp. 194
Author(s):  
Mosad Ahmed Ghareeb ◽  
Manal Mortady Hamed ◽  
Abdel-aleem Hassan Abdel-aleem ◽  
Amal Mohamed Saad ◽  
Mohamed Sayed Abdel-aziz ◽  
...  
Keyword(s):  
Essential Oil ◽  
Radical Scavenging ◽  
Agar Plate ◽  
Antimicrobial Activities ◽  
Butanol Extract ◽  
Syzygium Jambos ◽  
Isolation And Characterization ◽  
Total Antioxidant ◽  
Total Oil

Objectives: Over the past few decades, phenolic compounds become important due to it has been associated with protection against different diseasesand sensory point of vision. Hence, at the present study, there has been a growing interest to carry out structural elucidation and characterization ofthe pure isolates from Syzygium jambos.Methods: S. jambos dried powder leaves were extracted by soaking in 85% methanol solvent at room temperature 25±2°C. The antioxidative activityof the isolates was assessed according to 2,2’-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum assays.Results: A total of 8 compounds were isolated from the n-butanol extract of S. jambos (Family Myrtaceae) plant, they were identified as quercetin-3-O-rutinoside (1), prenylbenzoic acid 4-β-D-glucoside (2), morolic acid 3-O-caffeate (3), 5,4’-dihydroxy, 7-methoxy, 6-methyl-flavone (4),3,4,5-trihydroxybenzoic acid (5), quercetin (6), isoetin-7-O-β-D-glucopyranoside (7), and (4’-hydroxy-3’-methoxyphenol-β-D-[6-O-(4”-hydroxy-3”,5”-dimethoxylbenzoate)] glucopyranoside) (8). Compounds 5 and 6 showed the most radical scavengers among the tested compounds with SC50 values of5.50 and 4.30 μg/ml, respectively, compared to ascorbic acid as standard and the total antioxidant capacity (TAC) values of 605.0 and 680.59 mg ascorbicacid equivalent/g compound, respectively. In vitro antimicrobial activities of the isolated compounds were tested using disc agar plate method againstfour pathogenic microbial strains including Gram-positive, Gram-negative bacteria and yeast with inhibition zones from 9 to 19 mm. Gas chromatographymassspectrometry analysis for the essential oil provides twenty four identified components representing 92% of its total oil composition.Conclusion: The results supported that S. jambos could be attributed to sources of natural antioxidant and antimicrobial applications.

scholarly journals Determination of Chemical Composition, In Vitro and In Silico Evaluation of Essential Oil from Leaves of Apium graveolens Grown in Saudi Arabia

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7372
Author(s):  
Ahmed I. Foudah ◽  
Mohammed H. Alqarni ◽  
Aftab Alam ◽  
Mohammad Ayman Salkini ◽  
Pravej Alam ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Essential Oil ◽  
In Silico ◽  
Docking Simulation ◽  
Antimicrobial Activities ◽  
Apium Graveolens ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Anti Inflammatory

The aim of this study was to explore the composition and evaluate the in silico and in vitro antioxidants and antimicrobial and anti-inflammatory effects of Apium graveolens var. dulce leaves essential oil (AGO) collected from Al-Kharj (Saudi Arabia). AGO was isolated using the hydro-distillation method, and its composition was studied using gas-chromatography-mass Spectrometry (GC–MS), antimicrobial activities using well diffusion assay, and antioxidant and anti-inflammatory activities using spectrophotometric methods. The pharmacological activities of their major compounds were predicted using PASS (prediction of activity spectra for substances) and drug-likening properties by ADME (absorption, distribution, metabolism, and excretion) through web-based online tools. Isocnidilide (40.1%) was identified as the major constituent of AGO along with β-Selinene, Senkyunolide A, Phytyl acetate, and 3-Butylphthalide. AGO exhibited a superior antibacterial activity, and the strongest activity was detected against Gram-positive bacteria and Candida albicans. Additionally, it exhibited a weaker antioxidant potential and stronger anti-inflammatory effects. PASS prediction supported the pharmacological finding, whereas ADMET revealed the safety of AGO. The molecular docking of isocnidilide was carried out for antibacterial (DNA gyrase), antioxidant (tyrosinase), and anti-inflammatory (cyclooxygenase-2) activities. The docking simulation results were involved hydrophilic interactions and demonstrated high binding affinity of isocnidilide for anti-inflammatory protein (cycloxygenase-2). The presence of isocnidilide makes AGO a potential anti-inflammatory and antimicrobial agent. AGO, and its major metabolite isocnidilide, may be a suitable candidate for the future drug development.

scholarly journals In Vitro Antimicrobial Activity of Essential Oil Extracted from Leaves of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le in Vietnam

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 453 ◽  
Author(s):  
Nhan Trong Le ◽  
Duc Viet Ho ◽  
Tuan Quoc Doan ◽  
Anh Tuan Le ◽  
Ain Raal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Antiviral Effects ◽  
Scientific Report ◽  
Main Components ◽  
Hiv 1

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.

scholarly journals Chemical Composition and Biological Activity of the Essential Oil from Jamaican Cleome serrata

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Megil J. McNeil ◽  
Roy B. R. Porter ◽  
Lawrence A. D. Williams
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Oil Composition ◽  
Filter Paper Disc ◽  
Aerial Parts ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oil obtained from the aerial parts of Cleome serrata by hydrodistillation was analyzed by employing GC-FID, GC-MS and RI. Fourteen compounds comprising 90.4% of the total oil composition were characterized. The main components identified were ( Z)-phytol (53.0%) and di(2-ethylhexyl)-phthalate (DEHP) (14.7%). The oil was evaluated for its in vitro antimicrobial activities against nine pathogenic microorganisms using the filter paper disc diffusion method. Moderate antimicrobial activity was observed against five of the pathogens assayed. In addition, the essential oil was tested against the sweet potato weevil, Cylas formicarius elegantulus. Strong knockdown insecticidal activity was observed.

scholarly journals Composition, in vitro Anti-inflammatory, Antioxidant and Antimicrobial Activities of the Leaf Essential Oil of Machilus konishii from Taiwan

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Yu-Chang Su ◽  
Kuan-Ping Hsu ◽  
Chen-Lung Ho
Keyword(s):  
Essential Oil ◽  
Antimicrobial Activities ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Leaf Oil ◽  
Leaf Essential Oil ◽  
Anti Inflammatory ◽  
Main Components ◽  
Antioxidant And Antimicrobial Activities

The chemical composition and in vitro anti-inflammatory, antioxidant and antimicrobial activities of the leaf essential oil of Machilus konishii has been investigated. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC–FID and GC–MS. Sixty-six compounds were identified, representing 100% of the oil. The main components identified were α-pinene (33.9%), β-pinene (13.9%), and thymol (12.0%). The leaf oil was able to reduce nitric oxide production by lipopolysaccharide-activated murine macrophages RAW 264.7 without reducing the cell viability. In addition, the leaf oil showed strong antioxidant and antimicrobial activities. The major ingredient of the oil that was responsible for the anti-inflammatory, antioxidant and antimicrobial activities was thymol.

scholarly journals Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

2008 ◽  
Vol 25 (No. 2) ◽  
pp. 81-89 ◽  
Author(s):  
A. Adiguzel ◽  
H. Ozer ◽  
H. Kilic ◽  
B. Cetin
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Satureja Hortensis ◽  
Bacteria And Fungi

The present work reports the <i>in vitro</i> antimicrobial activities of the essential oil and methanol extract from <i>Satureja hortensis</i> as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), &gamma;-terpinene (18.56%), carvacrol (13.98%), and <i>p</i>-cymene (8.97). The essential oil of <i>Satureja hortensis</i> exhibited the activity against 25 bacteria, 8 fungi, and a yeast, <i>C. albicans</i>; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 &micro;l/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

Sign in / Sign up

Export Citation Format

Share Document