Elderberry Stalks as a Source of High-Value Phytochemical: Essential Minerals and Lipophilic Compounds

Elderberry (Sambucus nigra L.) consumption has been growing in the last years, generating a large number of stalks (~10% of the berries bunch) that are still under-valorized. This study focused on the evaluation of elderberry stalks as a source of high-value phytochemicals. In this vein, the essential mineral content and lipophilic composition were analyzed for the first time. In addition, the polar fraction was evaluated regarding its total phenolic content (TPC) and antioxidant activity by both 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2-diphenyl-1-picrylhydrazyl hydrate (DPPH) assays. The lipophilic fraction was mainly composed of triterpenic acids (2902.20 mg kg−1 of dry weight (dw)), fatty acids (711.73 mg kg−1 dw) and sterols (288.56 mg kg−1 dw). Minor amounts of long-chain aliphatic alcohols and other components were also detected. Ursolic acid (2265.83 mg kg−1 dw), hexadecanoic acid (219.85 mg kg−1 dw) and β-sitosterol (202.74 mg kg−1 dw) were the major lipophilic components verified. The results of this study also indicated that elderberry stalks might be used as a natural source of essential minerals, particularly calcium, iron and potassium, which are known to play important roles in various body functions. The analysis of the polar fraction also showed that elderberry stalks present TPC as high as elderberry themselves as well as considerable antioxidant activity (1.04 and 0.37 mmol TE g−1 of extract, against respectively ABTS and DPPH radicals). These results highlight the potential of elderberry stalks as a natural source of high-value phytochemicals that may be explored in several fields.

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Antioxidant Activity and Anthocyanin Contents in Olives (cv Cellina di Nardò) during Ripening and after Fermentation

Antioxidants ◽

10.3390/antiox8050138 ◽

2019 ◽

Vol 8 (5) ◽

pp. 138 ◽

Cited By ~ 5

Author(s):

Alessio Aprile ◽

Carmine Negro ◽

Erika Sabella ◽

Andrea Luvisi ◽

Francesca Nicolì ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Dry Weight ◽

Olive Tree ◽

Total Phenolic ◽

Table Olives ◽

Ripening Stages ◽

Black Olive ◽

First Time ◽

Black Color

The olive tree “Cellina di Nardò” (CdN) is one of the most widespread cultivars in Southern Italy, mainly grown in the Provinces of Lecce, Taranto, and Brindisi over a total of about 60,000 hectares. Although this cultivar is mainly used for oil production, the drupes are also suitable and potentially marketable as table olives. When used for this purpose, olives are harvested after complete maturation, which gives to them a naturally black color due to anthocyanin accumulation. This survey reports for the first time on the total phenolic content (TPC), anthocyanin characterization, and antioxidant activity of CdN olive fruits during ripening and after fermentation. The antioxidant activity (AA) was determined using three different methods. Data showed that TPC increased during maturation, reaching values two times higher in completely ripened olives. Anthocyanins were found only in mature olives and the concentrations reached up to 5.3 g/kg dry weight. AA was determined for the four ripening stages, and was particularly high in the totally black olive fruit, in accordance with TPC and anthocyanin amounts. Moreover, the CdN olives showed a higher TPC and a greater AA compared to other black table olives produced by cultivars commonly grown for this purpose. These data demonstrate the great potential of black table CdN olives, a product that combines exceptional organoleptic properties with a remarkable antioxidant capacity.

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PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY GLYCYRRHIZA PALLIDIFLORA HERB (GLYCYRRHIZA PALLIDIFLORA MAXIM.)

"Medical & pharmaceutical journal "Pulse" ◽

10.26787/nydha-2686-6838-2021-23-5-42-46 ◽

2021 ◽

pp. 42-46

Author(s):

Maltseva E.M. ◽

Egorova I.N. ◽

Pinchuk L.G.

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Russian Far East ◽

Far East ◽

Biologically Active ◽

Perennial Herb ◽

Total Phenolic ◽

Plant Antioxidants ◽

Phytochemical Studies ◽

First Time

Pale-flowered licorice (Glycyrrhiza pallidiflora Maxim.) is the pea family (Fabaceae) perennial herb. It is a Russian Far East endemic. Pale-flowered licorice refers to the false (not sweet) licorice section (Pseudoglycyrrhiza Krug.), which do not accumulate glycyrrhizin derivatives. The G. pallidiflora successful introduction has been carried out over the past 5 years in the “Apothecary garden” territory, FRC UUH SB RAS, Kemerovo. Phytochemical studies screening biologically active compounds main classes of the plant aerial part confirmed the presence of a complex of phenolic compounds. The content of catechin derivatives and condensed-type tannins - proanthocyanidins (PAC) data in G. pallidiflora herb were obtained for the first time. In different years of cultivation, licorice herb accumulates phenolic compounds - up to 2.83 ± 0.22% in terms of gallic acid, flavonoids - up to 2.44 ± 0.03% in terms of rutin and PAC in terms of cyanidine chloride - up to 2.61 ± 0.11%. It was found that the maximum content of the phenolic compounds and PAC sum was observed in the herb for 4 years development. The greatest number of flavonoids accumulates in the herb harvested in the 3rd year of cultivation. A positive significant linear relation was found between antioxidant activity (AOA) and the total phenolic compounds and PAC content (r≥0.98). Considering that BAC with IC values ≤ 50 μg / ml in the DPPH assay refer to active antioxidant’s licorice herb can be classified as a plant with high antioxidant potential. It was found that the iron chelating activity of the G. pallidiflora herb is in direct dependence (r≥0.94) on the content of flavonoids. The obtained results demonstrate the importance of further study of this plant as a source of BAC, including plant antioxidants.

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Effects of water stress on phenolic content and antioxidant activity of African nightshades

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f150204 ◽

2017 ◽

Vol 15 (2) ◽

pp. 79-95 ◽

Cited By ~ 1

Author(s):

ODHIAMBO PETER OKELLO ◽

JOSEPH P. ONYANGO GWEYI ◽

MILDRED PAULINE NAWIRI ◽

WINFRED MUSILA

Keyword(s):

Antioxidant Activity ◽

Water Stress ◽

Leaf Area ◽

Total Phenolic Content ◽

Phenolic Content ◽

Dry Weight ◽

Total Phenolic ◽

Stress Levels ◽

Total Antioxidant ◽

Solanum Villosum

Okello OP, Gweyi JPO, Nawiri Mp, Musila W. 2017. Effects of water stress on phenolic contents and antioxidant activity of African nightshades. Biofarmasi J Nat Prod Biochem 15: 79-95. This study aimed at mapping out the distribution of different African Nightshade species in Siaya and Kisii Counties of Kenya and to determine water stress effect on total antioxidant capacity and total phenolic content of two selected African nightshade namely, giant nightshade (Solanum scabrum) and black nightshade (Solanum villosum). Before selection of the two varieties, the study involved farmer field visits, mapping of nightshades present and administration of semi-structured questionnaires to farmers to determine the indigenous vegetables being grown, the nightshade species grown and factors affecting their production. The experiments were conducted both in the field and greenhouse conditions. Watering intervals were at 15 cbars, 50 cbars, and 85 cbars. Data on the number of secondary buds, leaf area, shoot height, shoot and root dry weights were gathered. The total antioxidant capacity and the total phenolic content were recorded using DPPH radical scavenging method and Folin-Ciolcalteu method, respectively. The data collected were subjected to ANOVA. In both counties where production was 100% under small scale, Solanum scabrum was the main variety grown is Siaya County (36%) while in Kisii the main variety was Solanum villosum (32%). There were significant differences (Pâ‰¤0.05) among treatments in leaf area, plant height, shoot biomass, number of secondary buds, leaf and root total phenolic content and leaf and root antioxidant activity. At all stress levels, Solanum scabrum exhibited the tallest plants with a maximum height of 45.17cm at 15cbars, while Solanum villosum had the shortest plants at all stress levels, with the shortest one being recorded at 16.65 cm at 85 cbars. S. scabrum also had the highest root dry weight (7.78g), shoot dry weight (50.78g) and highest leaf area of (304.45cm2). However, Solanum villosum had the highest number of secondary buds at all stress levels with the highest being 24 at 15 cbars. Concerning phytochemicals, Solanum villosum had a higher concentration of both the total phenolics and antioxidant activity in the shoots (46.41g GAE/Kg DM total phenolic content and 52.68% total antioxidant activity). Meanwhile, Solanum scabrum had a higher concentration in the roots (25.06gGAE/Kg DM total phenolic content and 27.18% total antioxidant activity). Water stress cause a decline in all growth parameters but increased phytochemical accumulation in nightshade accessions grown. It is therefore suggested that for better yields, irrigation should be performed at every 15 cbars, however for adequate phytochemical accumulation, the irrigation should be carried out at 50 cbars. Further research to explore and quantify other phytochemical components as affected by different watering regimes need to be undertaken.

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Biocomponents and Antioxidant Activity of Ribes khorasanicum

International journal of basic science in medicine ◽

10.15171/ijbsm.2018.18 ◽

2018 ◽

Vol 3 (3) ◽

pp. 99-103 ◽

Cited By ~ 7

Author(s):

Mohammad Ehsan Taghavizadeh Yazdi ◽

Jalil Khara ◽

Mohammad Reza Housaindokht ◽

Hamid Reza Sadeghnia ◽

Sedigheh Esmaeilzadeh Bahabadid ◽

...

Keyword(s):

Antioxidant Activity ◽

Sugar Content ◽

Antioxidant Properties ◽

Dry Weight ◽

Total Phenolic ◽

Anthocyanin Content ◽

Bioactive Components ◽

Total Anthocyanin ◽

Total Anthocyanin Content ◽

Biological And Medical Applications

Currently, there have been lots of interests in phytochemicals as bioactive components. The roles of fruit, vegetables, and red pigments in preventing diseases have been partly accredited to the antioxidant properties of their constituent polyphenols, flavonoid, anthocyanins, and etc. Biochemistry parameters including the relative levels of antioxidant activity, total phenolic content, total flavonoid, total anthocyanin, soluble and insoluble sugar content of Ribes khorasanicum, have been calculated in this project. The total anthocyanin content of the investigated Ribes khorasanicum measured to be 62.9 mg/g in dry weight, while displaying high levels when compared to other components, which indicates that the anthocyanin content was the predominant antioxidant components in the investigated plant. This particular plant has the potential to serve as the important source of antioxidant that can be utilized in different biological and medical applications.

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The Content of Selected Minerals, Bioactive Compounds, and the Antioxidant Properties of the Flowers and Fruit of Selected Cultivars and Wildly Growing Plants of Sambucus nigra L.

Molecules ◽

10.3390/molecules25040876 ◽

2020 ◽

Vol 25 (4) ◽

pp. 876 ◽

Cited By ~ 4

Author(s):

Karolina Młynarczyk ◽

Dorota Walkowiak-Tomczak ◽

Halina Staniek ◽

Marcin Kidoń ◽

Grzegorz P. Łysiak

Keyword(s):

Antioxidant Activity ◽

Mineral Content ◽

Antioxidant Properties ◽

Absorption Spectrometry ◽

Wild Plants ◽

Total Phenolic ◽

Anthocyanin Content ◽

Spectrophotometric Methods ◽

Bioactive Properties ◽

High Concentrations

This study compared the mineral content and bioactive properties of flowers and fruit coming from wild elderberry plants with those of flowers and fruit harvested from elderberry cultivars grown in an orchard. Elderberry fruit and flowers were analyzed for the content of selected minerals, phenolic compounds, and anthocyanins, as well as for antioxidant activity. Mineral content was determined by the atomic absorption spectrometry method, while antioxidant activity and the content of polyphenols and anthocyanins were determined by spectrophotometric methods. Flowers were found to contain more total ash and much higher content of most of minerals, except magnesium which was present in high concentrations in fruit. Fruit showed significantly higher antioxidant activity than flowers, whereas the total phenolic content varied depending on the growing location or cultivar. The material obtained from selected cultivars growing in an orchard had higher antioxidant activity and polyphenol and anthocyanin content than the material obtained from wild plants. Fruit of the ”Haschberg” cultivar and flowers of the ”Sampo” cultivar had the best bioactive properties of the studied samples.

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The Effect of Heating Temperature on Total Phenolic Content, Antioxidant Activity, and Phenolic Compounds of Plum and Mahaleb Fruits

International Journal of Food Engineering ◽

10.1515/ijfe-2017-0302 ◽

2019 ◽

Vol 15 (11-12) ◽

Cited By ~ 2

Author(s):

Kashif Ghafoor ◽

Isam A. Mohamed Ahmed ◽

Süleyman Doğu ◽

Nurhan Uslu ◽

Gbemisola J. Fadimu ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Gallic Acid ◽

Total Phenolic Content ◽

Phenolic Content ◽

Heating Temperature ◽

Dry Weight ◽

Total Phenolic ◽

Dihydroxybenzoic Acid ◽

Different Temperatures

AbstractThe effect of heating at different temperatures (60, 80, 90, 110, and 130 °C) on the total phenolic content, antioxidant activity, and phenolic compounds present in plum and mahaleb fruits was investigated. The antioxidant activity values and total phenolic contents of fresh plum (93.82% measured by DPPH method, 787.79 mg gallic acid equivalent (GAE)/100 g dry weight determined by Folin method) and mahaleb fruits (81.80%, 634.47 mg GAE/100 g dry weight) were higher than plum and mahaleb fruits dried at different temperatures (p < 0.05). Generally, the heating process caused a reduction in both total phenolic content and antioxidant activity for plum and mahaleb. While (+)-catechin (92.62 mg/kg), 1,2-dihydroxybenzene (132.15 mg/kg), gallic acid (107.01 mg/kg), and 3,4-dihydroxybenzoic acid (74.59 mg/kg) are the key phenolic compounds in fresh plum, (+)-catechin, 3,4-dihydroxybenzoic acid, 1,2-dihydroxybenzene, and syringic and caffeic acids were the major phenolic compounds of mahaleb fruits. The polyphenol content of fruits and the class of phenolics present are significantly affected by heating temperature.

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Five-Golden-Flowers Tea: Green Extraction and Hepatoprotective Effect against Oxidative Damage

Molecules ◽

10.3390/molecules23092216 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2216 ◽

Cited By ~ 7

Author(s):

Cai-Ning Zhao ◽

Guo-Yi Tang ◽

Qing Liu ◽

Xiao-Yu Xu ◽

Shi-Yu Cao ◽

...

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

High Performance ◽

Antioxidant Properties ◽

Dry Weight ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Herbal Tea ◽

Antioxidant Power

The consumption of herbal teas has become popular in recent years due to their attractive flavors and outstanding antioxidant properties. The Five-Golden-Flowers tea is a herbal tea consisting of five famous edible flowers. The effects of microwave-assisted extraction parameters on the antioxidant activity of Five-Golden-Flowers tea were studied by single-factor experiments, and further investigated using response surface methodology. Under the optimal parameters (53.04 mL/g of solvent/material ratio, 65.52 °C, 30.89 min, and 500 W), the ferric-reducing antioxidant power, Trolox equivalent antioxidant capacity, and total phenolic content of the herbal tea were 862.90 ± 2.44 µmol Fe2+/g dry weight (DW), 474.37 ± 1.92 µmol Trolox/g DW, and 65.50 ± 1.26 mg gallic acid equivalent (GAE)/g DW, respectively. The in vivo antioxidant activity of the herbal tea was evaluated on alcohol-induced acute liver injury in mice. The herbal tea significantly decreased the levels of aspartate aminotransferase, total bilirubin, and malonaldehyde at different doses (200, 400, and 800 mg/kg); improved the levels of liver index, serum triacylglycerol, and catalase at dose of 800 mg/kg. These results indicated its role in alleviating hepatic oxidative injury. Besides, rutin, chlorogenic acid, epicatechin, gallic acid, and p-coumaric acid were identified and quantified by high performance liquid chromatography (HPLC), which could contribute to the antioxidant activity of the herbal tea.

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Fruit Stalk Extract from Chili Peppers (Capsicum annum L.) as a Natural Antioxidant to Inhibit Oxidation in Crude Palm Oil

Jurnal Kimia Sains dan Aplikasi ◽

10.14710/jksa.23.4.124-128 ◽

2020 ◽

Vol 23 (4) ◽

pp. 124-128

Author(s):

Rahmiwati Hilma ◽

Eka Tri Setya Wulandari ◽

Zaldi Arman

Keyword(s):

Fatty Acids ◽

Antioxidant Activity ◽

Free Fatty Acids ◽

Iodine Number ◽

Palm Oil ◽

Dry Weight ◽

Chili Pepper ◽

Total Phenolic ◽

Crude Palm Oil ◽

Capsicum Annum

Fruit stalk of chili pepper (Capsicum annum L.) so far is still an untapped waste. The content of active compounds in fruit and fruit stalk of chili pepper (C. annum L.) is known to have good antioxidant activity. The purpose of this study was to evaluate the extract of fruit stalk of chili pepper in inhibiting the oxidation of crude palm oil (CPO). In this study, the extraction of fruit stalk of chili pepper (C. annum L.) was carried out with n-hexane (from now on referred to as CHE) solvent and with ethanol solvent (from now on referred to as CEE). CHE and CEE were analyzed for total phenolic and flavonoid analyzes. Next, an evaluation of antioxidant activity was carried out using the DPPH method. The effectiveness test of adding the two extracts to the quality of CPO was carried out for ten days using a Completely Randomized Design (CRD) analysis. The parameters observed were peroxide numbers, free fatty acids, and iodine numbers. Data were analyzed by ANOVA and followed by a Least Significance Different (LSD) test. The results show that the total phenolic value is 0.257 mg QE/g dry weight in CEE and 0.003 mg QE/g dry weight in CHE. Total flavonoid values are 0.155 mg QE/g dry weight in CEE and 0.003 mg QE/g in CHE. IC50 values for DPPH test extract are 223.72 μg/mL in CEE and 953.77 μg/mL in CHE. The test results of the effectiveness of the two extracts against CPO show thin CEE, and CHE significantly (P <0.05) influences to CPO free fatty acids, peroxide number, and iodine number. Both extracts can protect CPO from oxidation. CEE is more effective in maintaining CPO quality for ten days with free fatty acid values (2.1%), peroxide value values 0.48 meq/kg, and iodine number 54.8. Furthermore, this result meets the CPO quality standard, according to SNI-01-2901-2006.

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Carlina vulgaris L. as a Source of Phytochemicals with Antioxidant Activity

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/1891849 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Maciej Strzemski ◽

Magdalena Wójciak-Kosior ◽

Ireneusz Sowa ◽

Daniel Załuski ◽

Wojciech Szwerc ◽

...

Keyword(s):

Antioxidant Activity ◽

Chlorogenic Acid ◽

18Th Century ◽

Dry Weight ◽

Biologically Active ◽

Total Phenolic ◽

13Th Century ◽

Primary And Secondary Metabolites ◽

Open Cast Mining ◽

Antioxidant Potency

The methanol extracts from three populations of Carlina vulgaris L. were examined for the chlorogenic acid content, mineral content, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity. Two populations originated from natural nonmetallicolous habitats (NN (populations from Nasiłów) and NP (populations from Pińczów)), and one metallicolous population (MB) was collected from Bolesław waste heap localized at the place of former open-cast mining of Ag-Pb and Zn-Pb ores dating back to the 13th century and 18th century, respectively. The level of Zn, Pb, Cd, Fe, Ni, and Mn was significantly higher in the root and leaves of MB plants as a result of soil contaminations compared to those of the NN and NP ones. The highest antioxidant potency has been showed by the plants growing in a nonmetallicolous habitat. The flower head extracts obtained from the nonmetallicolous populations also contained the largest amount of chlorogenic acid, whereas the lowest was determined in the roots (ca. 2–3.5 mg/g and 0.2–0.4 mg/g of air-dry weight, resp.). These studies provide important information on the influence of a habitat on the quality of herbal materials and the content of the biologically active primary and secondary metabolites.

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Abts cation scavenging activity and total phenolic content of three moss species

Hemijska industrija ◽

10.2298/hemind120131022p ◽

2012 ◽

Vol 66 (5) ◽

pp. 723-726 ◽

Cited By ~ 4

Author(s):

Boris Pejin ◽

Jelena Bogdanovic-Pristov

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Scavenging Activity ◽

Moss Species ◽

Phenolic Contents ◽

Antioxidant Agents ◽

Acid Cation ◽

First Time

A limited number of mosses have ever been studied in regard to antioxidant activity; with only few belonging to the European species. As part of our on-going screening of bryophytes as natural antioxidant resources, a relevant biological activity investigation was conducted on the three moss species, namely Brachythecium rutabulum (Hedw.) Schimp. (Brachytheciaceae), Calliergonella cuspidata (Hedw.) Loeske (Hypnaceae) and Hypnum mammillatum (Brid.) Loeske (Hypnaceae), collected in Germany. The antioxidant activity of corresponding lyophilised aqueous extracts (teas) was evaluated on the basis of their ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)] cation scavenging activities. The total phenolic contents were determined as well. This study led to the detection of a moderate total phenolic content of the moss B. rutabulum, indicating for the first time its potential in searching for novel antioxidant agents.

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