scholarly journals Quadruplex-Forming Motif Inserted into 3′UTR of Ty1his3-AI Retrotransposon Inhibits Retrotransposition in Yeast

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 347
Author(s):  
Viktor Tokan ◽  
Jose Luis Rodriguez Rodriguez Lorenzo ◽  
Pavel Jedlicka ◽  
Iva Kejnovska ◽  
Roman Hobza ◽  
...  
Keyword(s):  
Life Cycle ◽  
Reverse Transcription ◽  
Dna Conformation ◽  
Mrna Levels ◽  
Template Strand ◽  
Yeast Strains ◽  
G4 Dna ◽  
Pif1 Helicase ◽  
The Impact ◽  
Eukaryotic Genomes

Guanine quadruplexes (G4s) serve as regulators of replication, recombination and gene expression. G4 motifs have been recently identified in LTR retrotransposons, but their role in the retrotransposon life-cycle is yet to be understood. Therefore, we inserted G4s into the 3′UTR of Ty1his3-AI retrotransposon and measured the frequency of retrotransposition in yeast strains BY4741, Y00509 (without Pif1 helicase) and with G4-stabilization by N-methyl mesoporphyrin IX (NMM) treatment. We evaluated the impact of G4s on mRNA levels by RT-qPCR and products of reverse transcription by Southern blot analysis. We found that the presence of G4 inhibited Ty1his3-AI retrotransposition. The effect was stronger when G4s were on a transcription template strand which leads to reverse transcription interruption. Both NMM and Pif1p deficiency reduced the retrotransposition irrespective of the presence of a G4 motif in the Ty1his3-AI element. Quantity of mRNA and products of reverse transcription did not fully explain the impact of G4s on Ty1his3-AI retrotransposition indicating that G4s probably affect some other steps of the retrotransposon life-cycle (e.g., translation, VLP formation, integration). Our results suggest that G4 DNA conformation can tune the activity of mobile genetic elements that in turn contribute to shaping the eukaryotic genomes.

Alternative splicing in plants

2008 ◽  
Vol 36 (3) ◽  
pp. 508-510 ◽  
Author(s):  
Craig G. Simpson ◽  
Dominika Lewandowska ◽  
John Fuller ◽  
Monika Maronova ◽  
Maria Kalyna ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alternative Splicing ◽  
Reverse Transcription ◽  
Protein Function ◽  
Mrna Levels ◽  
Reverse Transcription Pcr ◽  
Multiple Genes ◽  
The Impact

The impact of AS (alternative splicing) is well-recognized in animal systems as a key regulator of gene expression and proteome complexity. In plants, AS is of growing importance as more genes are found to undergo AS, but relatively little is known about the factors regulating AS or the consequences of AS on mRNA levels and protein function. We have established an accurate and reproducible RT (reverse transcription)–PCR system to analyse AS in multiple genes. Initial studies have identified new AS events confirming that current values for the frequency of AS in plants are likely to be underestimates.

Generation of Heme Oxygenase-1-Transgenic Rats

2003 ◽  
Vol 228 (5) ◽  
pp. 466-471 ◽  
Author(s):  
C. Braudeau ◽  
D. Bouchet ◽  
C. Toquet ◽  
L. Tesson ◽  
S. Ménoret ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Immune Responses ◽  
Heme Oxygenase ◽  
Reverse Transcription ◽  
Heme Oxygenase 1 ◽  
Mrna Levels ◽  
Transgenic Rats ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
The Impact

Heme oxygenase-1 (HO-1) expression protects cells from a variety of cellular insults and inhibits inflammation. However, its role in the regulation of immune responses has not yet been clearly established. We generated HO-1 transgenic rats to directly test the impact of HO-1 on the different immune mechanisms. To temporally control the expression of HO-1, we used a one-plasmid tetracycline (tet)-inducible system. This plasmid contains the H-2Kb promoter, which transcribes the tet transactivator (tTA) and expression of a human HO-1 cDNA is obtained in the absence of tetracycline. The DNA construct was microinjected into one-cell rat embryos and mothers and pups were maintained with tetracycline. Eight transgenic founders were obtained. Analysis of transgene expression in the absence of tet showed that 2 lines (12.4 and 12.6) expressed HO-1 mRNA in several organs (as detected by reverse transcription polymerase chain reaction) and at the protein level only in the thymus. Expression levels of transgene-derived HO-1 increased after withdrawal of tet compared with transgenic rats maintained with tet, as detected by analysis of mRNA levels by quantitative real-time reverse transcription polymerase chain reaction. Gross examination and histopathological analysis of several organs in both lines showed no anomalies. Thymocytes and splenocytes of both lines showed normal cell subpopulations and allogeneic proliferation compared with controls. Systemic immune responses against cognate antigens were normal in both lines, as evaluated by the proliferation of lymph node cells and the production of antibodies against keyhole limpet hemocyanin after immunization. Animals from line 12.6 rejected transplanted allogeneic hearts with the same kinetics as controls. In conclusion, short-term induction of HO-1 overexpression did not modify immune responses compared to those of control non-transgenic animals.

Life-Cycle Costs of Selected Uniformed Health Professions (Phase II: The Impact of Constraints and Policies on the Optimal-Mix-of-Accession Model)

2003 ◽  
Author(s):  
Shayne Brannman ◽  
Eric W. Christensen ◽  
Ronald H. Nickel ◽  
Cori Rattelman ◽  
Richard D. Miller
Keyword(s):  
Life Cycle ◽  
Phase Ii ◽  
Health Professions ◽  
Life Cycle Costs ◽  
Optimal Mix ◽  
The Impact

scholarly journals How Different Fermentation Type Affects Volatile Composition of Plum Jerkums

2021 ◽  
Vol 11 (10) ◽  
pp. 4658
Author(s):  
Magdalena Januszek ◽  
Paweł Satora
Keyword(s):  
Volatile Compounds ◽  
Current Paper ◽  
Higher Alcohols ◽  
High Quality ◽  
Volatile Composition ◽  
Spontaneous Fermentation ◽  
Fermentation Efficiency ◽  
Yeast Strains ◽  
The Impact

Quality of plum jerkum is significantly associated to the profile of volatile compounds. Therefore, we decided to assess the impact of various fermentation types on selected properties of plum jerkums, especially compounds which contribute to the aroma of the finished product. We used the following yeast strains: S. cerevisiae S1, H. uvarum H2, and Ethanol RED (S. cerevisiae). Moreover, we considered spontaneous fermentation. S. cerevisiae and H. uvarum strains were isolated during the fermentation of Čačanska Lepotica or Węgierka Dąbrowicka (plum cultivars), respectively. As for fermentation type, spontaneous fermentation of H. uvarum H2 provided the best results. It could be associated to the fact that plum juices fermented with H. uvarum H2 presented the highest concentration of terpenoids, esters, or some higher alcohols. In the current paper, application of indigenous strains of yeasts resulted in the required oenological characteristics, e.g., highest fermentation efficiency and concentration of ethanol was determined in juices fermented with Ethanol RED (S. cerevisiae) and also with S. cerevisiae S1. Our results suggested that indigenous strains of yeasts present in plums demonstrate great potential for the production of plum jerkums of high quality.

scholarly journals 2-Piece Cork Stoppers as Alternative for Valorization of Thin Cork Planks: Analysis by LCA Methodology

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 873
Author(s):  
Francisco Javier Flor-Montalvo ◽  
Agustín Sánchez-Toledo Ledesma ◽  
Eduardo Martínez Cámara ◽  
Emilio Jiménez-Macías ◽  
Jorge Luis García-Alcaraz ◽  
...  
Keyword(s):  
Life Cycle Assessment ◽  
Life Cycle ◽  
Raw Materials ◽  
Lca Methodology ◽  
Significant Difference ◽  
Different Dimensions ◽  
The Impact ◽  
Different Thickness ◽  
Cork Stoppers ◽  
Wine Bottle

Natural stoppers are a magnificent closure for the production of aging wines and unique wines, whose application is limited by the availability of raw materials and more specifically of cork sheets of different thickness and quality. The growing demand for quality wine bottle closures leads to the search for alternative stopper production. The two-piece stopper is an alternative since it uses non-usable plates in a conventional way for the production of quality caps. The present study has analyzed the impact of the manufacture of these two-piece stoppers using different methodologies and for different dimensions by developing an LCA (Life Cycle Assessment), concluding that the process phases of the plate, its boiling, and its stabilization, are the phases with the greatest impact. Likewise, it is detected that the impacts in all phases are relatively similar (for one kg of net cork produced), although the volumetric difference between these stoppers represents a significant difference in impacts for each unit produced.

scholarly journals Case study of a water bioengineering construction site in Austria. Ecological aspects and application of an environmental life cycle assessment model

2021 ◽  
Author(s):  
M. von der Thannen ◽  
S. Hoerbinger ◽  
C. Muellebner ◽  
H. Biber ◽  
H. P. Rauch
Keyword(s):  
Global Warming ◽  
Life Cycle Assessment ◽  
Life Cycle ◽  
Energy Demand ◽  
Assessment Model ◽  
Construction Site ◽  
Negative Effects ◽  
Environmental Life Cycle Assessment ◽  
The Impact ◽  
Soil And Water

AbstractRecently, applications of soil and water bioengineering constructions using living plants and supplementary materials have become increasingly popular. Besides technical effects, soil and water bioengineering has the advantage of additionally taking into consideration ecological values and the values of landscape aesthetics. When implementing soil and water bioengineering structures, suitable plants must be selected, and the structures must be given a dimension taking into account potential impact loads. A consideration of energy flows and the potential negative impact of construction in terms of energy and greenhouse gas balance has been neglected until now. The current study closes this gap of knowledge by introducing a method for detecting the possible negative effects of installing soil and water bioengineering measures. For this purpose, an environmental life cycle assessment model has been applied. The impact categories global warming potential and cumulative energy demand are used in this paper to describe the type of impacts which a bioengineering construction site causes. Additionally, the water bioengineering measure is contrasted with a conventional civil engineering structure. The results determine that the bioengineering alternative performs slightly better, in terms of energy demand and global warming potential, than the conventional measure. The most relevant factor is shown to be the impact of the running machines at the water bioengineering construction site. Finally, an integral ecological assessment model for applications of soil and water bioengineering structures should point out the potential negative effects caused during installation and, furthermore, integrate the assessment of potential positive effects due to the development of living plants in the use stage of the structures.

scholarly journals Life Cycle Assessment of an Innovative Hybrid Energy Storage System for Residential Buildings in Continental Climates

2021 ◽  
Vol 11 (9) ◽  
pp. 3820
Author(s):  
Noelia Llantoy ◽  
Gabriel Zsembinszki ◽  
Valeria Palomba ◽  
Andrea Frazzica ◽  
Mattia Dallapiccola ◽  
...  
Keyword(s):  
Life Cycle Assessment ◽  
Life Cycle ◽  
Residential Buildings ◽  
Storage System ◽  
Energy Storage System ◽  
Hot Water ◽  
Hybrid Energy ◽  
Hybrid Energy Storage ◽  
Innovative System ◽  
The Impact

With the aim of contributing to achieving the decarbonization of the energy sector, the environmental impact of an innovative system to produce heating and domestic hot water for heating demand-dominated climates is assessed is evaluated. The evaluation is conducted using the life cycle assessment (LCA) methodology and the ReCiPe and IPCC GWP indicators for the manufacturing and operation stages, and comparing the system to a reference one. Results show that the innovative system has a lower overall impact than the reference one. Moreover, a parametric study to evaluate the impact of the refrigerant is carried out, showing that the impact of the overall systems is not affected if the amount of refrigerant or the impact of refrigerant is increased.

scholarly journals Life Cycle Assessment (LCA) of an Innovative Compact Hybrid Electrical-Thermal Storage System for Residential Buildings in Mediterranean Climate

2021 ◽  
Vol 13 (9) ◽  
pp. 5322
Author(s):  
Gabriel Zsembinszki ◽  
Noelia Llantoy ◽  
Valeria Palomba ◽  
Andrea Frazzica ◽  
Mattia Dallapiccola ◽  
...  
Keyword(s):  
Life Cycle Assessment ◽  
Life Cycle ◽  
Environmental Impact ◽  
Mediterranean Climate ◽  
Residential Buildings ◽  
Renewable Energy Sources ◽  
Storage System ◽  
Hot Water ◽  
Electrical Consumption ◽  
The Impact

The buildings sector is one of the least sustainable activities in the world, accounting for around 40% of the total global energy demand. With the aim to reduce the environmental impact of this sector, the use of renewable energy sources coupled with energy storage systems in buildings has been investigated in recent years. Innovative solutions for cooling, heating, and domestic hot water in buildings can contribute to the buildings’ decarbonization by achieving a reduction of building electrical consumption needed to keep comfortable conditions. However, the environmental impact of a new system is not only related to its electrical consumption from the grid, but also to the environmental load produced in the manufacturing and disposal stages of system components. This study investigates the environmental impact of an innovative system proposed for residential buildings in Mediterranean climate through a life cycle assessment. The results show that, due to the complexity of the system, the manufacturing and disposal stages have a high environmental impact, which is not compensated by the reduction of the impact during the operational stage. A parametric study was also performed to investigate the effect of the design of the storage system on the overall system impact.

scholarly journals Advances of 2nd Life Applications for Lithium Ion Batteries from Electric Vehicles Based on Energy Demand

2021 ◽  
Vol 13 (10) ◽  
pp. 5726
Author(s):  
Aleksandra Wewer ◽  
Pinar Bilge ◽  
Franz Dietrich
Keyword(s):  
Life Cycle ◽  
Lithium Ion Batteries ◽  
Electric Vehicles ◽  
Energy Demand ◽  
Lithium Ion ◽  
Life Cycles ◽  
Future Research ◽  
Research Areas ◽  
Study Results ◽  
The Impact

Electromobility is a new approach to the reduction of CO2 emissions and the deceleration of global warming. Its environmental impacts are often compared to traditional mobility solutions based on gasoline or diesel engines. The comparison pertains mostly to the single life cycle of a battery. The impact of multiple life cycles remains an important, and yet unanswered, question. The aim of this paper is to demonstrate advances of 2nd life applications for lithium ion batteries from electric vehicles based on their energy demand. Therefore, it highlights the limitations of a conventional life cycle analysis (LCA) and presents a supplementary method of analysis by providing the design and results of a meta study on the environmental impact of lithium ion batteries. The study focuses on energy demand, and investigates its total impact for different cases considering 2nd life applications such as (C1) material recycling, (C2) repurposing and (C3) reuse. Required reprocessing methods such as remanufacturing of batteries lie at the basis of these 2nd life applications. Batteries are used in their 2nd lives for stationary energy storage (C2, repurpose) and electric vehicles (C3, reuse). The study results confirm that both of these 2nd life applications require less energy than the recycling of batteries at the end of their first life and the production of new batteries. The paper concludes by identifying future research areas in order to generate precise forecasts for 2nd life applications and their industrial dissemination.

scholarly journals Processes, Challenges and Optimisation of Rum Production from Molasses—A Contemporary Review

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 21
Author(s):  
Tinashe Mangwanda ◽  
Joel B. Johnson ◽  
Janice S. Mani ◽  
Steve Jackson ◽  
Shaneel Chandra ◽  
...  
Keyword(s):  
Management Practices ◽  
Scientific Literature ◽  
Asia Pacific ◽  
Yeast Strains ◽  
Challenges And Opportunities ◽  
The Caribbean ◽  
The Impact ◽  
Production Industry ◽  
Stringent Quality

The rum industry is currently worth USD 16 billion, with production concentrated in tropical countries of the Caribbean and Asia-Pacific regions. The primary feedstock for rum production is sugar cane molasses, a by-product of sugar refineries. The main variables known to affect rum quality include the composition of the molasses, the length of fermentation, and the type of barrels and length of time used for aging the rum. The goal of this review is to provide an overview of the impact of these variables on rum quality, and to highlight current challenges and opportunities in the production of rum from molasses. In order to achieve this, we review the relevant contemporary scientific literature on these topics. The major contemporary challenges in the rum production industry include minimising the effects of variability in feedstock quality, ensuring the fermentation process runs to completion, preventing microbial contamination, and the selection and maintenance of yeast strains providing optimum ethanol production. Stringent quality management practices are required to ensure consistency in the quality and organoleptic properties of the rum from batch to batch. Further research is required to fully understand the influences of many of these variables on the final quality of the rum produced.

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