scholarly journals Phenotype Switching in Metal-Tolerant Bacteria Isolated from a Hyperaccumulator Plant

Biology ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 879
Author(s):  
Artur Banach ◽  
Agnieszka Kuźniar ◽  
Anna Marzec-Grządziel ◽  
Anna Gałązka ◽  
Agnieszka Wolińska
Keyword(s):  
Plant Growth ◽  
Organic Compounds ◽  
Growth Promotion ◽  
Stress Factors ◽  
Dual Function ◽  
Azolla Filiculoides ◽  
Auxin Production ◽  
Promote Plant Growth ◽  
Achromobacter Sp ◽  
Metal Tolerant Bacteria

As an adaptation to unfavorable conditions, microorganisms may represent different phenotypes. Azolla filiculoides L. is a hyperaccumulator of pollutants, but the functions of its microbiome have not been well recognized to date. We aimed to reveal the potential of the microbiome for degradation of organic compounds, as well as its potential to promote plant growth in the presence of heavy metals. We applied the BiologTM Phenotypic Microarrays platform to study the potential of the microbiome for the degradation of 96 carbon compounds and stress factors and assayed the hydrolytic potential and auxin production by the microorganisms in the presence of Pb, Cd, Cr (VI), Ni, Ag, and Au. We found various phenotype changes depending on the stress factor, suggesting a possible dual function of the studied microorganisms, i.e., in bioremediation and as a biofertilizer for plant growth promotion. Delftia sp., Staphylococcus sp. and Microbacterium sp. exhibited high efficacy in metabolizing organic compounds. Delftia sp., Achromobacter sp. and Agrobacterium sp. were efficient in enzymatic responses and were characterized by metal tolerant. Since each strain exhibited individual phenotype changes due to the studied stresses, they may all be beneficial as both biofertilizers and bioremediation agents, especially when combined in one biopreparation.

scholarly journals Isolation and selection of plant growth-promoting bacteria associated with sugarcane

2016 ◽  
Vol 46 (2) ◽  
pp. 149-158 ◽  
Author(s):  
Ariana Alves Rodrigues ◽  
Marcus Vinicius Forzani ◽  
Renan de Souza Soares ◽  
Sergio Tadeu Sibov ◽  
José Daniel Gonçalves Vieira
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Vital Role ◽  
Plant Growth Promoting Bacteria ◽  
Biotic And Abiotic Stress ◽  
Phosphate Solubilizers ◽  
Chitinase A ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

ABSTRACT Microorganisms play a vital role in maintaining soil fertility and plant health. They can act as biofertilizers and increase the resistance to biotic and abiotic stress. This study aimed at isolating and characterizing plant growth-promoting bacteria associated with sugarcane, as well as assessing their ability to promote plant growth. Endophytic bacteria from leaf, stem, root and rhizosphere were isolated from the RB 867515 commercial sugarcane variety and screened for indole acetic acid (IAA) production, ability to solubilize phosphate, fix nitrogen and produce hydrogen cyanide (HCN), ammonia and the enzymes pectinase, cellulase and chitinase. A total of 136 bacteria were isolated, with 83 of them presenting some plant growth mechanism: 47 % phosphate solubilizers, 26 % nitrogen fixers and 57 % producing IAA, 0.7 % HCN and chitinase, 45 % ammonia, 30 % cellulose and 8 % pectinase. The seven best isolates were tested for their ability to promote plant growth in maize. The isolates tested for plant growth promotion belong to the Enterobacteriaceae family and the Klebsiella, Enterobacter and Pantoea genera. Five isolates promoted plant growth in greenhouse experiments, showing potential as biofertilizers.

Stimulation of the ionic transport system in Brassica napus by a plant growth-promoting rhizobacterium (Achromobacter sp.)

2000 ◽  
Vol 46 (3) ◽  
pp. 229-236 ◽  
Author(s):  
H Bertrand ◽  
C Plassard ◽  
X Pinochet ◽  
B Touraine ◽  
P Normand ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Root System ◽  
Oilseed Rape ◽  
Root Zone ◽  
Growth Promotion ◽  
Mineral Nutrient ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Achromobacter Sp

A plant growth-promoting rhizobacterium belonging to the genus Achromobacter was isolated from the oilseed-rape (Brassica napus) root. Growth promotion bioassays were performed with oilseed rape seedlings in a growth chamber in test tubes containing attapulgite and mineral nutrient solution, containing NO3- as N source. The presence of this Achromobacter strain increased shoot and root dry weight by 22-33% and 6-21%, respectively. Inoculation of young seedlings with the Achromobacter bacteria induced a 100% improvement in NO3- uptake by the whole root system. Observations on the seminal root of seedlings 20 h after inoculation showed that there was an enhancement of both the number and the length of root hairs, compared to non-inoculated seedlings. Electrophysiological measurements of NO3- net flux with ion-selective microelectrodes showed that inoculation resulted in a specific increase of net nitrate flux in a root zone morphologically similar in inoculated and non-inoculated plants. The root area increased due to root hair stimulation by the Achromobacter bacteria, which might have contributed to the improvement of NO3- uptake by the whole root system, together with the enhancement of specific NO3- uptake rate. Moreover, inoculated plants showed increased potassium net influx and proton net efflux. Overall, the data presented suggest that the inoculation of oilseed-rape with the bacteria Achromobacter affects the mineral uptake.Key words: Brassica napus, plant growth-promoting rhizobacteria, Achromobacter sp., mineral uptake, root morphology.

scholarly journals Bacterial Formulations in Induction of Resistance and Growth Promotion of Tomato Plants

2018 ◽  
Vol 10 (10) ◽  
pp. 493
Author(s):  
José R. M. Campos Neto ◽  
Rafael Ribeiro Chaves ◽  
Diogo Herison Silva Sardinha ◽  
Luiz Gustavo de Lima Melo ◽  
Antônia Alice Costa Rodrigues
Keyword(s):  
Plant Growth ◽  
Fusarium Wilt ◽  
Seed Treatment ◽  
Dry Matter ◽  
Growth Promotion ◽  
Systemic Resistance ◽  
Tomato Plants ◽  
Vigor Index ◽  
Promote Plant Growth ◽  
Induction Of Resistance

The objective of this work was to evaluate the effectiveness of seed treatment with fresh suspensions and powder formulations with Bacillus methylotrophicus to promote plant growth and induction of resistance against fusarium wilt (Fusarium oxysporum f. sp. lycopersici) in tomato plants under greenhouse conditions, verifying the occurrence of morphological and biochemical changes in the evaluated plants. Powder formulations based on Cassava (Manihot esculenta), Arrowroot (Maranta arundinacea) and sodium alginate containing Bacillus, in addition to the commercial product Quartz®, were used to microbiolize the tomato seeds of the cultivar Santa Cruz. The formulations promoted plant growth, with a seedling vigor index greater than 50% for all treatments containing B. mthylotrophicus, in addition to a significant increase in total dry matter. The treatments induced systemic resistance, controlling the fusarium wilt with a 75% reduction of the disease and activation of enzymes such as peroxidase and polyphenoloxidase, only β-1,3-glucanase presented less activity than controls (treatments without B. mthylotrophicus). Thus, the use of formulations containing Bacillus are efficient in promoting plant growth of tomato plants and in inducing resistance to the control of fusarium wilt.

scholarly journals Effects of Talaromyces purpureogenus on Cucumber Growth Promotion and Its Mechanism

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Zhao L ◽  
◽  
Zhao W ◽  
Deng H ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Scientific Basis ◽  
Promoting Effect ◽  
Iaa Production ◽  
Significant Difference ◽  
Application Potential ◽  
Promote Plant Growth ◽  
Growth Promoting

Some fungi may promote plant growth by production of siderophores, Indole Acetic Acid (IAA) and phosphorus dissolving capability. In this study, eight fungi were isolated from the mushroom substrate, and their siderophores production, IAA production and phosphorus dissolving traits were determined. Although there was no significant difference in IAA production among the eight fungi, but the strain M13026-2 was a fungus with strong growth promoting traits compared with other seven fungi. In order to study the correlation between the growth promoting effect of cucumber pot culture and the above three traits, five fungi with different strength of traits were tested in pot. As a result, M13026- 2 which was identified as Talaromyces purpureogenus could significantly improve the growth parameters of cucumber seedlings, and could colonize in the rhizosphere soil and the tissue of cucumber stably. All the results suggested that the most relevant to their ability to promote plant growth is the trait of phosphorus dissolving, followed by siderophores production. The results of this study will provide scientific basis for the efficient selection and identification of a large number of fungi resources with the function of promoting plant growth, and reveal the good application potential of T. purpureogenus in agriculture fields.

scholarly journals Plant Growth Promoting Abilities of Novel Burkholderia-Related Genera and Their Interactions With Some Economically Important Tree Species

2021 ◽  
Vol 5 ◽  
Author(s):  
Munusamy Madhaiyan ◽  
Govindan Selvakumar ◽  
Tan HianHwee Alex ◽  
Lin Cai ◽  
Lianghui Ji
Keyword(s):  
Plant Growth ◽  
Oil Palm ◽  
Tree Species ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Acc Deaminase ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Promote Plant Growth

A survey of bacterial endophytes associated with the leaves of oil palm and acacias resulted in the isolation of 19 bacterial strains belonging to the genera Paraburkholderia, Caballeronia, and Chitinasiproducens, which are now regarded as distinctively different from the parent genus Burkholderia. Most strains possessed one or more plant growth promotion (PGP) traits although nitrogenase activity was present in only a subset of the isolates. The diazotrophic Paraburkholderia tropica strain S39-2 with multiple PGP traits and the non-diazotrophic Chitinasiproducens palmae strain JS23T with a significant level of 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity were selected to investigate the influence of bacterial inoculation on some economically important tree species. Microscopic examination revealed that P. tropica S39-2 was rhizospheric as well as endophytic while C. palmae JS23T was endophytic. P. tropica strain S39-2 significantly promoted the growth of oil palm, eucalyptus, and Jatropha curcas. Interestingly, the non-diazotrophic, non-auxin producing C. palmae JS23T strain also significantly promoted the growth of oil palm and eucalyptus although it showed negligible effect on J. curcas. Our results suggest that strains belonging to the novel Burkholderia-related genera widely promote plant growth via both N-independent and N-dependent mechanisms. Our results also suggest that the induction of defense response may prevent the colonization of an endophyte in plants.

Promotion of plant growth and inhibition of enzymic degradation of indole-3-acetic acid by metabolites of carbofuran, a carbamate insecticide

1977 ◽  
Vol 55 (5) ◽  
pp. 574-579 ◽  
Author(s):  
T. T. Lee
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Inhibitory Action ◽  
Critical Level ◽  
Growth Promotion ◽  
Stem Segment ◽  
Stem Tissue ◽  
Carbamate Insecticide ◽  
Promote Plant Growth ◽  
Indole 3 Acetic Acid

The carbamate insecticide carbofuran (2,2-dimethyl-2,3-dihydrobenzofuranyl-7-N-methyl carbamate) and three of its metabolites (7-hydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (III), 3,7-dihydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (IV), and 3-keto-7-hydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (V)) stimulated growth in the pea stem segment assay in the presence, but not absence, of a low concentration of indole-3-acetic acid (IAA). The metabolites were more active than carbofuran itself. The synergistic effect on growth was specific with IAA since it was not observed in the presence of other auxins.Metabolites III, IV, and V and, to a lesser degree, carbofuran were found to be inhibitory to IAA degradation catalyzed by pea stem tissue or purified horseradish peroxidase (EC 1.11.1.7). Comparison of the relative activities of the compounds in the inhibition of IAA degradation and in the promotion of plant growth suggests a causal relationship. The implication is that carbofuran may promote plant growth through the inhibitory action of its metabolites on enzymic breakdown of IAA, thus preserving a critical level of IAA required for growth promotion.

scholarly journals The Pseudomonas Secondary Metabolite 2,4-Diacetylphloroglucinol Is a Signal Inducing Rhizoplane Expression of Azospirillum Genes Involved in Plant-Growth Promotion

2011 ◽  
Vol 24 (2) ◽  
pp. 271-284 ◽  
Author(s):  
Emeline Combes-Meynet ◽  
Joël F. Pothier ◽  
Yvan Moënne-Loccoz ◽  
Claire Prigent-Combaret
Keyword(s):  
Plant Growth ◽  
Secondary Metabolite ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Fluorescence Induction ◽  
Auxin Production ◽  
Wide Range ◽  
Enhanced Expression ◽  
Plant Growth Promoting ◽  
Negative Mutant

During evolution, plants have become associated with guilds of plant-growth-promoting rhizobacteria (PGPR), which raises the possibility that individual PGPR populations may have developed mechanisms to cointeract with one another on plant roots. We hypothesize that this has resulted in signaling phenomena between different types of PGPR colonizing the same roots. Here, the objective was to determine whether the Pseudomonas secondary metabolite 2,4-diacetylphloroglucinol (DAPG) can act as a signal on Azospirillum PGPR and enhance the phytostimulation effects of the latter. On roots, the DAPG-producing Pseudomonas fluorescens F113 strain but not its phl-negative mutant enhanced the phytostimulatory effect of Azospirillum brasilense Sp245-Rif on wheat. Accordingly, DAPG enhanced Sp245-Rif traits involved in root colonization (cell motility, biofilm formation, and poly-β-hydroxybutyrate production) and phytostimulation (auxin production). A differential fluorescence induction promoter-trapping approach based on flow cytometry was then used to identify Sp245-Rif genes upregulated by DAPG. DAPG enhanced expression of a wide range of Sp245-Rif genes, including genes involved in phytostimulation. Four of them (i.e., ppdC, flgE, nirK, and nifX-nifB) tended to be upregulated on roots in the presence of P. fluorescens F113 compared with its phl-negative mutant. Our results indicate that DAPG can act as a signal by which some beneficial pseudomonads may stimulate plant-beneficial activities of Azospirillum PGPR.

scholarly journals Quantifying Plant-Borne Carbon Assimilation by Root-Associating Bacteria

2020 ◽  
Vol 8 (5) ◽  
pp. 700 ◽  
Author(s):  
Spenser Waller ◽  
Stacy L. Wilder ◽  
Michael J. Schueller ◽  
Alexandra B. Housh ◽  
Richard A. Ferrieri
Keyword(s):  
Plant Growth ◽  
Fluorescent Protein ◽  
Growth Promotion ◽  
Carbon Assimilation ◽  
Microbial Colonization ◽  
Fluorescence Measurement ◽  
Grass Root ◽  
Promote Plant Growth ◽  
The One ◽  
Green Fluorescent

Herbaspirillum seropedicae is a rhizobacteria that occupies a specialized ecological niche in agriculture. As an endophyte and prolific grass root colonizer it has the potential to promote plant growth, enhancing crop yield in many cereal crops. While the mechanisms for plant growth promotion are controversial, the one irrefutable fact is these microorganisms rely heavily on plant-borne carbon as their main energy source in support of their biological functions. Unfortunately, the tools and technology enabling researchers to trace carbon exchange between plants and the microorganisms associating with them has been limiting. Here, we demonstrate that radioactive 11CO2 administered to intact maize leaves with translocation of 11C-photosynthates to roots can provide a ‘traceable’ source of carbon whose assimilation by microbial organisms can be quantified with enormous sensitivity. Fluorescence root imaging of RAM10, a green fluorescent protein (GFP) reporting strain of H. seropedicae, was used to identify regions of high microbial colonization. Microbes were mechanically removed from these regions via sonication in saline solution and extracts were subjected to fluorescence measurement and gamma counting to correlate carbon-11 atoms with numbers of colony forming units. The method has potential to translate to other microorganisms provided they possess an optical reporting trait.

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