scholarly journals Effect of Olive Cake and Cactus Cladodes Incorporation in Goat Kids’ Diet on the Rumen Microbial Community Profile and Meat Fatty Acid Composition

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1237
Author(s):  
Samira El Otmani ◽  
Youssef Chebli ◽  
Bernard Taminiau ◽  
Mouad Chentouf ◽  
Jean-Luc Hornick ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Microbial Community ◽  
Control Diet ◽  
Alpha Diversity ◽  
Olive Cake ◽  
Feed Resources ◽  
Alternative Feed ◽  
Southern Mediterranean ◽  
The Individual ◽  
Microbial Community Profile

The olive cake (OC) and the cactus cladodes (CC) are two alternative feed resources widely available in the southern Mediterranean region that could be used in ruminants’ diet. Their impact on the rumen bacterial ecosystem is unknown. This work aims to evaluate their effects on the microbial community and meat fatty acids of goat’s kids. Forty-four goat kids were divided into four groups receiving diets with conventional concentrate, or 35% OC, or 30% CC, or 15% OC, and 15% CC. After 3 months, these animals were slaughtered, and the rumen liquor and longissimus dorsi and semimembranosus muscles samples were collected. Animals receiving a control diet had rumen liquor with high acidity than test groups (p < 0.001). Test rumen liquor was more adapted to digest efficiently their matching diet than control liquor (p < 0.05). These feedstuffs did not affect rumen bacteria abundance and alpha diversity (richness, evenness, and reciprocal Simpson indexes), and these results were confirmed by beta-diversity tests (NMDS plot, HOMOVA, PERMANOVA). The test diets slightly affected the individual fatty acids of meat (p < 0.05) without effect on fatty acids summaries, indexes, and ratios. Thus, these alternative feed resources could take place in goat kids’ diet to diversify their feed and to reduce feed costs.

Effect of rumen-protected supplements of fish oil on intake, digestibility and nitrogen balance of growing goats

2004 ◽  
Vol 79 (3) ◽  
pp. 483-491 ◽  
Author(s):  
J. R. Fernández ◽  
M. Rodríguez Osorio ◽  
E. Ramos ◽  
G. de la Torre ◽  
F. Gil Extremera ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Dry Matter ◽  
Control Diet ◽  
Live Weight ◽  
Control Group ◽  
Partial Hydrogenation ◽  
Acid Detergent Fibre ◽  
The Individual ◽  
Growing Goats

AbstractTwo groups of six male goats were used to assess the effects of rumen-protected supplements offish oil on intake, digestibility and nitrogen (N) balance. The animals were offered a diet consisting of forage and concentrate, the latter fraction supplemented with 0 (control) or lOOg/kg of rumen-protected fish oil supplement (PFO), containing a high proportion of the n-3 series (whole diet contained 0 or 60 g PFO per kg dry matter). No significant differences (P > 0.05) were found between the two groups concerning live-weight gain, food intake, digestibility of DM, organic matter, N, neutral-detergent fibre and energy. In contrast, there were differences (P < 0.05) regarding the digestibility of fat and of acid-detergent fibre, which were higher among the animals given the PFO diet. With respect to the individual fatty acids, we observed higher digestibility (P < 0.05) of C14:0, C16:0, C18:0 and C20:0 among the animals given the PFO diet. The digestibility of C14:0, C18:0 and C20:0 was found to be negative among the animals given the control diet. No significant differences (P > 0-05) were found regarding digestibility of total C18:1. In contrast, the coefficients for C18:2 (n-6) and C18:3 (n-3) were higher (P < 0.05) among the non-supplemented animals. The intake and faecal flow values of C18:0 suggest that the mono- and polyunsaturated fatty acids with 18 atoms of carbon may, in both cases, undergo partial hydrogenation, which would be greater among the control group. The utilization of C20:5 (n-3) and, especially, of C22 : 6 (n-3), which were consumed only by the animals given the PFO diet, was estimated at 1-000. The PFO diet also produced lower levels ofurinary-N excretion (P < 0.05), giving rise to higher N balances (P < 0.05).

Chemical composition and in vitro digestibility of alternative feed resources for ruminants in Mediterranean climates: olive cake and cactus cladodes

2019 ◽  
Vol 157 (03) ◽  
pp. 260-271 ◽  
Author(s):  
S. El Otmani ◽  
M. Chentouf ◽  
J. L. Hornick ◽  
J. F. Cabaraux
Keyword(s):  
Chemical Composition ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Olive Cake ◽  
Two Phase ◽  
Feed Resources ◽  
Alternative Feed ◽  
Mechanical Press ◽  
Extraction Period

AbstractOlive cake (OC) and cactus cladodes (CCs) are two alternative feed resources widely available in Mediterranean areas. Their use in ruminant diets was assessed according to their chemical composition, secondary compound levels and digestibility. The effects of the olive oil extraction period and process, and CCs age and sampling period were evaluated. OC was collected monthly, from November to January, from mills using either a mechanical press or 2-phase or 3-phase centrifugation processes. CCs were collected fortnightly according to age (young and mature) from April to June. Two-phase OC had the lowest content of dry matter (DM), the highest nitrogen-free extract (NFE) and total and hydrolysable tannins and was more rapidly fermentable. Mechanical press OC was the least digestible. OC DM, protein and NFE were affected linearly by the extraction period. Gas production (GP), in vitro digestibility parameters and dry and organic enzymatic digestibility changed with the extraction period. Therefore, OC chemical composition and in vitro digestibility depended mainly on the extraction process and period. Compared to mature CCs , young CCs contained more water, protein, ether-extract and phenolic compounds, but less ash and fibre. GP and digestibility parameters were not affected by age, but in vitro organic matter digestibility and microbial biomass production were higher in young cladodes. CCs chemical composition, GP and digestibility parameters were influenced by the collection period. Due to its limited nutritional quality, OC should be enriched in nitrogen, while CCs could be considered as highly valuable forage in ruminant diet.

scholarly journals Comparative Analysis of Fecal Microbiota of Grazing Mongolian Cattle from Different Regions in Inner Mongolia, China

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1938
Author(s):  
Han Aricha ◽  
Huasai Simujide ◽  
Chunjie Wang ◽  
Jian Zhang ◽  
Wenting Lv ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Gut Microbiota ◽  
Inner Mongolia ◽  
Alpha Diversity ◽  
Fecal Microbiota ◽  
Composition Analysis ◽  
Geographical Factors ◽  
Rrna Sequencing ◽  
Xilingol Grassland

Mongolian cattle from China have strong adaptability and disease resistance. We aimed to compare the gut microbiota community structure and diversity in grazing Mongolian cattle from different regions in Inner Mongolia and to elucidate the influence of geographical factors on the intestinal microbial community structure. We used high throughput 16S rRNA sequencing to analyze the fecal microbial community and diversity in samples from 60 grazing Mongolian cattle from Hulunbuir Grassland, Xilingol Grassland, and Alxa Desert. A total of 2,720,545 high-quality reads and sequences that were 1,117,505,301 bp long were obtained. Alpha diversity among the three groups showed that the gut microbial diversity in Mongolian cattle in the grasslands was significantly higher than that in the desert. The dominant phyla were Firmicutes and Bacteroidetes, whereas Verrucomicrobia presented the highest abundance in the gut of cattle in the Alxa Desert. The gut bacterial communities in cattle from the grasslands versus the Alxa Desert were distinctive, and those from the grasslands were closely clustered. Community composition analysis revealed significant differences in species diversity and richness. Overall, the composition of the gut microbiota in Mongolian cattle is affected by geographical factors. Gut microbiota may play important roles in the geographical adaptations of Mongolian cattle.

scholarly journals Microbiota Changes in Fathers Consuming a High Prebiotic Fiber Diet Have Minimal Effects on Male and Female Offspring in Rats

Nutrients ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 820
Author(s):  
Faye Chleilat ◽  
Alana Schick ◽  
Raylene A. Reimer
Keyword(s):  
Beta Diversity ◽  
Control Diet ◽  
Gastrointestinal Hormones ◽  
Alpha Diversity ◽  
Female Offspring ◽  
Sprague Dawley ◽  
Microbial Composition ◽  
Gut Hormone ◽  
Satiety Hormone ◽  
Prebiotic Fiber

Background: Consuming a diet high in prebiotic fiber has been associated with improved metabolic and gut microbial parameters intergenerationally, although studies have been limited to maternal intake with no studies examining this effect in a paternal model. Method: Male Sprague Dawley rats were allocated to either (1) control or (2) oligofructose-supplemented diet for nine weeks and then mated. Offspring consumed control diet until 16 weeks of age. Bodyweight, body composition, glycemia, hepatic triglycerides, gastrointestinal hormones, and gut microbiota composition were measured in fathers and offspring. Results: Paternal energy intake was reduced, while satiety inducing peptide tyrosine tyrosine (PYY) gut hormone was increased in prebiotic versus control fathers. Increased serum PYY persisted in female prebiotic adult offspring. Hepatic triglycerides were decreased in prebiotic fathers with a similar trend (p = 0.07) seen in female offspring. Gut microbial composition showed significantly reduced alpha diversity in prebiotic fathers at 9 and 12 weeks of age (p < 0.001), as well as concurrent differences in beta diversity (p < 0.001), characterized by differences in Bifidobacteriaceae, Lactobacillaceae and Erysipelotrichaceae, and particularly Bifidobacterium animalis. Female prebiotic offspring had higher alpha diversity at 3 and 9 weeks of age (p < 0.002) and differences in beta diversity at 15 weeks of age (p = 0.04). Increases in Bacteroidetes in female offspring and Christensenellaceae in male offspring were seen at nine weeks of age. Conclusions: Although paternal prebiotic intake before conception improves metabolic and microbiota outcomes in fathers, effects on offspring were limited with increased serum satiety hormone levels and changes to only select gut bacteria.

scholarly journals Benchmarking laboratory processes to characterise low-biomass respiratory microbiota

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Raiza Hasrat ◽  
Jolanda Kool ◽  
Wouter A. A. de Steenhuijsen Piters ◽  
Mei Ling J. N. Chu ◽  
Sjoerd Kuiling ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Composition ◽  
Microbial Community Composition ◽  
Positive Control ◽  
Rrna Gene ◽  
Elution Buffer ◽  
Community Profile ◽  
Microbial Community Profile ◽  
Microbial Dna ◽  
Pcr Conditions

AbstractThe low biomass of respiratory samples makes it difficult to accurately characterise the microbial community composition. PCR conditions and contaminating microbial DNA can alter the biological profile. The objective of this study was to benchmark the currently available laboratory protocols to accurately analyse the microbial community of low biomass samples. To study the effect of PCR conditions on the microbial community profile, we amplified the 16S rRNA gene of respiratory samples using various bacterial loads and different number of PCR cycles. Libraries were purified by gel electrophoresis or AMPure XP and sequenced by V2 or V3 MiSeq reagent kits by Illumina sequencing. The positive control was diluted in different solvents. PCR conditions had no significant influence on the microbial community profile of low biomass samples. Purification methods and MiSeq reagent kits provided nearly similar microbiota profiles (paired Bray–Curtis dissimilarity median: 0.03 and 0.05, respectively). While profiles of positive controls were significantly influenced by the type of dilution solvent, the theoretical profile of the Zymo mock was most accurately analysed when the Zymo mock was diluted in elution buffer (difference compared to the theoretical Zymo mock: 21.6% for elution buffer, 29.2% for Milli-Q, and 79.6% for DNA/RNA shield). Microbiota profiles of DNA blanks formed a distinct cluster compared to low biomass samples, demonstrating that low biomass samples can accurately be distinguished from DNA blanks. In summary, to accurately characterise the microbial community composition we recommend 1. amplification of the obtained microbial DNA with 30 PCR cycles, 2. purifying amplicon pools by two consecutive AMPure XP steps and 3. sequence the pooled amplicons by V3 MiSeq reagent kit. The benchmarked standardized laboratory workflow presented here ensures comparability of results within and between low biomass microbiome studies.

scholarly journals The Influence of Diet and Sex on the Gut Microbiota of Lean and Obese JCR:LA-cp Rats

2021 ◽  
Vol 9 (5) ◽  
pp. 1037
Author(s):  
Craig Resch ◽  
Mihir Parikh ◽  
J. Alejandro Austria ◽  
Spencer D. Proctor ◽  
Thomas Netticadan ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Control Diet ◽  
Bacterial Species ◽  
Alpha Diversity ◽  
Short Chain Fatty Acids ◽  
Female Rats ◽  
Dependent Manner ◽  
Male And Female ◽  
Ground Flaxseed ◽  
The Impact

There is an increased interest in the gut microbiota as it relates to health and obesity. The impact of diet and sex on the gut microbiota in conjunction with obesity also demands extensive systemic investigation. Thus, the influence of sex, diet, and flaxseed supplementation on the gut microbiota was examined in the JCR:LA-cp rat model of genetic obesity. Male and female obese rats were randomized into four groups (n = 8) to receive, for 12 weeks, either (a) control diet (Con), (b) control diet supplemented with 10% ground flaxseed (CFlax), (c) a high-fat, high sucrose (HFHS) diet, or (d) HFHS supplemented with 10% ground flaxseed (HFlax). Male and female JCR:LA-cp lean rats served as genetic controls and received similar dietary interventions. Illumine MiSeq sequencing revealed a richer microbiota in rats fed control diets rather than HFHS diets. Obese female rats had lower alpha-diversity than lean female; however, both sexes of obese and lean JCR rats differed significantly in β-diversity, as their gut microbiota was composed of different abundances of bacterial types. The feeding of an HFHS diet affected the diversity by increasing the phylum Bacteroidetes and reducing bacterial species from phylum Firmicutes. Fecal short-chain fatty acids such as acetate, propionate, and butyrate-producing bacterial species were correspondingly impacted by the HFHS diet. Flax supplementation improved the gut microbiota by decreasing the abundance of Blautia and Eubacterium dolichum. Collectively, our data show that an HFHS diet results in gut microbiota dysbiosis in a sex-dependent manner. Flaxseed supplementation to the diet had a significant impact on gut microbiota diversity under both flax control and HFHS dietary conditions.

scholarly journals Straw-Based Biopurification Systems to Remove Ibuprofen, Diclofenac and Triclosan from Wastewaters: Dominant Microbial Communities

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1507
Author(s):  
Laura Delgado-Moreno ◽  
Pieter van Dillewijn ◽  
Rogelio Nogales ◽  
Esperanza Romero
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Point Source ◽  
Relative Abundance ◽  
Alpha Diversity ◽  
Microbial Structure ◽  
Methyl Triclosan ◽  
Source Contamination ◽  
Biopurification Systems ◽  
Olive Mill

The continued discharge of pharmaceuticals and personal care products (PPCPs) into the environment due to their widespread use and the lack of effective systems for their removal from water is a global problem. In this study, the dissipation of ibuprofen, diclofenac and triclosan added simultaneously in biopurification systems (BPSs) with different compositions and their effect on the microbial community structure was analysed. Three BPSs, constituted by mixtures of soil (S), peat (P), or raw wet olive mill cake (A) or its vermicompost (V) and straw (S) were prepared (SPS, SAS and SVS). Sorption and degradation experiments were carried out. After 84 days of incubation, more than 85% of each PPCP applied had dissipated. Methyl-triclosan was determined to be highest in the SVS biomixture. Biomixtures with lower C/N ratio and higher alpha diversity were the most effective in the removal of PPCPs. Initially, the BPS biomixtures showed a different microbial structure dominated by Proteobacteria, Actinobacteria and Bacteroidetes but after addition of PPCPs, a similar pattern was observed in the relative abundance of the phylum Chloroflexi, the class Sphingobacteriia and the genus Brevundimonas. These biopurification systems can be useful to prevent point source contamination due to the disposal of PPCP-contaminated waters.

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