scholarly journals RadA, a MSCRAMM Adhesin of the Dominant Symbiote Ruminococcus gnavus E1, Binds Human Immunoglobulins and Intestinal Mucins

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1613
Author(s):  
Marc Maresca ◽  
Radia Alatou ◽  
Ange Pujol ◽  
Cendrine Nicoletti ◽  
Josette Perrier ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Terminal Region ◽  
Glycan Structure ◽  
Protein Backbone ◽  
Bacterial Persistence ◽  
Human Immunoglobulins ◽  
Different Types ◽  
Key Factor ◽  
Competition Assays ◽  
Intestinal Mucins

Adhesion to the digestive mucosa is considered a key factor for bacterial persistence within the gut. In this study, we show that Ruminococcus gnavus E1 can express the radA gene, which encodes an adhesin of the MSCRAMMs family, only when it colonizes the gut. The RadA N-terminal region contains an all-β bacterial Ig-like domain known to interact with collagens. We observed that it preferentially binds human immunoglobulins (IgA and IgG) and intestinal mucins. Using deglycosylated substrates, we also showed that the RadA N-terminal region recognizes two different types of motifs, the protein backbone of human IgG and the glycan structure of mucins. Finally, competition assays with lectins and free monosaccharides identified Galactose and N-Acetyl-Galactosamine motifs as specific targets for the binding of RadA to mucins and the surface of human epithelial cells.

scholarly journals Supply Chain Coordination Contracts under Double Sided Disruptions Simultaneously

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Huan Zhang ◽  
Yang Liu ◽  
Jingsi Huang
Keyword(s):  
Supply Chain ◽  
Supply Chain Coordination ◽  
Optimal Price ◽  
Supply Chain System ◽  
Pricing Policy ◽  
Product Cost ◽  
Numerical Examples ◽  
Supply Chain Contracts ◽  
Different Types ◽  
Key Factor

Supply chain coordination models are developed in a two-echelon supply chain with double sided disruptions. In a supply chain system, the supplier may suffer from the product cost disruption and the retailer suffers from the demand disruption simultaneously. The purpose of this study is to design proper supply chain contracts, under which the supply chain with double sided disruption can be coordinated. Firstly, the centralized decision-making models are applied to find the optimal price and quantity under three cases as the baseline. The different cases are divided by the different relationship between the product cost disruption and the demand disruption. Secondly, two different types of contracts are introduced to coordinate the whole supply chain. One is all-unit wholesale quantity discount policy (AQDP) contract, and the other one is capacitated linear pricing policy (CLPP) contract. And it is found out that the gap between the demand disruption and the product cost disruption is the key factor to influence the supply chain coordination. Some numerical examples and sensitivity analysis are given to illustrate the models. The AQDP contracts are listed out under different cases to show how to use it under double sided disruptions.

scholarly journals Significant expression of FURIN and ACE2 on oral epithelial cells may facilitate the efficiency of SARS-CoV-2 entry

2020 ◽  
Author(s):  
Mei Zhong ◽  
Bing-peng Lin ◽  
Hong-bin Gao ◽  
Andrew J Young ◽  
Xin-hong Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oral Mucosa ◽  
Cardiac Tissue ◽  
Clinical Care ◽  
Host Cells ◽  
Mucosal Tissues ◽  
Mucosal Cells ◽  
Key Factor ◽  
Significant Expression ◽  
Epithelial Layers

AbstractBackgroundLeading to a sustained epidemic spread with >2,000,000 confirmed human infections, including >100,000 deaths, COVID-19 was caused by SARS-CoV-2 and resulted in acute respiratory distress syndrome (ARDS) and sepsis, which brought more challenges to the patient’s treatment. The S-glycoprotein, which recognized as the key factor for the entry of SARS-CoV-2 into the cell, contains two functional domains: an ACE2 receptor binding domain and a second domain necessary for fusion of the coronavirus and cell membranes. FURIN activity, exposes the binding and fusion domains, is essential for the zoonotic transmission of SARS-CoV-2. Moreover, it has been reported that ACE2 is likely to be the receptor for SARS-CoV-2. In addition, FURIN enzyme and ACE2 receptor were expressed in airway epithelia, cardiac tissue, and enteric canals, which considered as the potential target organ of the virus. However, report about the expression of FURIN and ACE2 in oral tissues was limited.MethodsIn order to investigate the potential infective channel of new coronavirus in oral cavity, we analyze the expression of ACE2 and FURIN that mediate the new coronavirus entry into host cells in oral mucosa using the public single-cell sequence datasets. Furthermore, immunohistochemical staining experiment was performed to confirm the expression of ACE2 and FURIN in the protein level.ResultsThe bioinformatics results indicated the differential expression of ACE2 and FURIN on epithelial cells of different oral mucosal tissues and the proportion of FURIN-positive cells was obviously higher than that of ACE2-positive cells. IHC experiments revealed that both the ACE2-positive and FURIN-positive cells in the target tissues were mainly positioned in the epithelial layers, partly expressed in fibroblasts, which further confirm the bioinformatics results.ConclusionsBased on these findings, we speculated that SARS-CoV-2 could effectively invade oral mucosal cells though two possible routes: binding to the ACE2 receptor and fusion with cell membrane activated by FURIN protease. Our results indicated that oral mucosa tissues are susceptible to SARS-CoV-2, which provides valuable information for virus-prevention strategy in clinical care as well as daily life.

scholarly journals Paracrine interactions between epithelial cells promote colon cancer growth

2020 ◽  
Author(s):  
Guillaume Jacquemin ◽  
Annabelle Wurmser ◽  
Mathilde Huyghe ◽  
Wenjie Sun ◽  
Meghan Perkins ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cancer Cells ◽  
Stromal Cells ◽  
Critical Role ◽  
Essential Factor ◽  
Transcriptional Responses ◽  
Tumour Formation ◽  
Different Types ◽  
The Impact

AbstractTumours are complex ecosystems composed of different types of cells that communicate and influence each other. While the critical role of stromal cells in affecting tumour growth is well established, the impact of mutant cancer cells on healthy surrounding tissues remains poorly defined. Here, we uncovered a paracrine mechanism by which intestinal cancer cells reactivate foetal and regenerative Yap-associated transcriptional programs in neighbouring wildtype epithelial cells, rendering them adapted to thrive in the tumour context. We identified the glycoprotein Thrombospondin-1 (Thbs1) as the essential factor that mediates non-cell autonomous morphological and transcriptional responses. Importantly, Thbs1 is associated with bad prognosis in several human cancers. This study reveals the Thbs1-YAP axis as the mechanistic link mediating paracrine interactions between epithelial cells, promoting tumour formation and progression.

Musical Changes Driven by Technology

2019 ◽  
pp. 103-120
Author(s):  
Peter Townsend
Keyword(s):  
Science And Technology ◽  
Wind Instruments ◽  
Visual Appearance ◽  
Musical Compositions ◽  
Different Types ◽  
Key Factor ◽  
New Instruments

This chapter explores how science and technology has revolutionized the sounds produced by different instruments. The main examples here are for the violin family and wind instruments. Some changes do not significantly alter the visual appearance, but allow different types of performance, more power, changes in tone, and totally new instruments (e.g. the saxophone). This has had an immense influence on composition, to exploit these new sounds, and of course an equally major swing in fashion from the audiences and players. Understanding of the science has been a key factor in the developments. Change is not always popular, and for example many players of early musical compositions believe they should be played with original sounding instruments and pitch. Unfortunately, the majority of audiences are now so accustomed to the new sounds that they find the earlier ones difficult to appreciate.

Nanovoid Formation at Cu/Cu/Cu Interconnections of Blind Microvias: A Field Study

2019 ◽  
Vol 2019 (1) ◽  
pp. 000492-000502 ◽  
Author(s):  
T. Bernhard ◽  
L. Gregoriades ◽  
S. Branagan ◽  
L. Stamp ◽  
E. Steinhäuser ◽  
...  
Keyword(s):  
Printed Circuit Boards ◽  
Structural Integrity ◽  
Weak Link ◽  
Critical Factor ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Different Types ◽  
Key Factor ◽  
Electrical Reliability ◽  
The Impact

Abstract A key factor for a high electrical reliability of multilayer High Density Interconnection Printed Circuit Boards (HDI PCBs) is the thermomechanical stability of stacked microvia interconnections. With decreasing via sizes and higher numbers of interconnected layers, the structural integrity of these interconnections becomes a critical factor and is a topic of high interest in current research. The formation of nanovoids and inhibited Cu recrystallization across the interfaces are the two main indications of a weak link from the target pad to the filled via. Based on TEM/EDX measurements on a statistically relevant number of stacked and blind microvias produced in the industrial field, different types of nanovoid phenomena are revealed at the Cu/Cu/Cu junction. The types of nanovoids were categorized relating to the time of appearance (before or after thermal treatment), the affected interfaces or layers and the impact on the Cu recrystallization. The main root causes for each void type are identified and the expected impact on the thermomechanical stability of the via junction is discussed.

scholarly journals Ultrastructure of blebbing and phagocytosis of blebs by hyperplastic thyroid epithelial cells in vivo.

1977 ◽  
Vol 72 (3) ◽  
pp. 584-594 ◽  
Author(s):  
J D Zeligs ◽  
S H Wollman
Keyword(s):  
Thyroid Gland ◽  
Epithelial Cells ◽  
Cultured Cells ◽  
Ultrastructural Organization ◽  
Contractile Ring ◽  
Phagocytic Process ◽  
Different Types ◽  
Rat Thyroid

In addition to pseudopods, somewhat pleomorphic blebs were consistently found protruding from the apical surfaces of hyperplastic rat thyroid epithelial cells into the follicular lumens in vivo. Many blebs were knobby, roughly hemispherical protrusions, with a diameter of 2-3 mum. Such blebs had a densely packed microfilamentous core and contained numerous apparent ribosomes. They were morphologically similar to blebs that have been observed in a variety of cultured cells. Other blebs were larger, more elongate, and less knobby, but had a similar ultrastructural organization. Blebs of all sizes appeared to be phagocytosed on some occasions by nearby epithelial cells. The phagocytic process involved partial engulfment of the bleb by a typical epithelial pseudopod, followed by an apparent pinching-off process, presumably resulting in the separation of the bleb from its cells or origin. The pinching-off process was associated with a band of approx. 6-nm diameter microfilaments that developed within the pseudopod cytoplasm surrounding the base of the bleb and is postulated to function as a contractile ring. The finding of blebbing is an intact tissue in vivo indicates that this phenomenon is not restricted to cultured cells, and thus tends to extend the significance of in vitro observations of the process. In relation to their occurrence in the hyperplastic thyroid gland in vivo, possible interconversions are considered between different types of blebs, and between blebs and pseudopods.

Obsessive Versus Delusional Jealousy: Destruction in a Form of Creation – A Review

2016 ◽  
Vol 33 (S1) ◽  
pp. S531-S531
Author(s):  
R. Almeida Leite ◽  
E. Conde ◽  
T. Queirós Santos ◽  
M. Almeida ◽  
T. Azevedo Santos ◽  
...  
Keyword(s):  
Behavioral Therapy ◽  
Obsessive Compulsive ◽  
Psychotherapeutic Interventions ◽  
Different Types ◽  
Key Factor ◽  
Compulsive Checking ◽  
The Common ◽  
Competing Interest ◽  
Delusional Jealousy

IntroductionJealousy is a complex emotional state and some degree is considered normal in mature love, but when does it become destructive in a relationship? There's a thin line between what is normal and what is pathologic. Pathological jealousy differs from normal by its intensity and irrationality. Obsessive and delusional jealousies are different types of pathological jealousy, difficult to distinguish, which is important, since they have different treatment. Despite the differences, both result in significant distress and carry the risk of homicide/suicide, so it's a matter deserving the psychiatrists’ attention.ObjectiveExplore the psychopathological differences between obsessive and delusional jealousy and list the characteristics and difficulties in the approach to pathological jealousy.MethodsThe results were obtained searching literature included on the PubMed and Google Scholar platforms.ResultsDelusional jealousy is characterized by strong and false beliefs that the partner is unfaithful. Individuals with obsessive jealousy suffer from unpleasant and irrational jealous ruminations that the partner could be unfaithful, accompanied by compulsive checking of partners’ behavior. This jealousy resembles obsessive-compulsive phenomenology and should be treated with SSRIs and cognitive-behavioral therapy. Delusional jealousy is a psychotic disorder and should be treated with antipsychotics.ConclusionThe common issue in pathological jealousy is the problem of adherence to treatment and bad prognosis. In order to achieve better treatment outcomes, we should follow-up the patient regularly. One key factor is to explore the psychopathology and motivate the sufferer for the proper pharmacological and psychotherapeutic interventions, trying to reduce the suffering caused by ideas of unfaithfulness.Disclosure of interestThe authors have not supplied their declaration of competing interest.

Out of the Barracks: The Role of the Military in Democratic Revolutions

2017 ◽  
Vol 45 (1) ◽  
pp. 78-100 ◽  
Author(s):  
Marcos Degaut
Keyword(s):  
South Korea ◽  
Necessary Condition ◽  
Tiananmen Square ◽  
Velvet Revolution ◽  
Different Types ◽  
Key Factor ◽  
Democratic Revolution ◽  
Action Theories ◽  
The Military

Why some democratic revolutions succeed while others fail? The scholarly community has sought to address this issue from various perspectives, from rational choice approaches to collective action theories. Too little attention, however, has been paid to analyzing the role of the military. By discussing the different types of interactions played by the military in five cases of successful democratic revolutions—the 1910 Portuguese Republican Revolution, the 1958 Venezuelan Revolution, the 1960 April Revolution in South Korea, the 1989 Velvet Revolution in Czechoslovakia, and the 2000 Bulldozer Revolution in Yugoslavia—and three cases of failed revolutions, the 1905 bourgeois-liberal revolution in Russia, the 1989 Tiananmen Square Protests in China, and the 2016 Turkey’s coup attempt, this study finds out that the key factor in determining their outcome is the army’s response and that the military backing is a necessary condition for a democratic revolution to succeed.

scholarly journals Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization

mBio ◽  
2015 ◽  
Vol 6 (4) ◽  
Author(s):  
Volker Winstel ◽  
Petra Kühner ◽  
Ferdinand Salomon ◽  
Jesper Larsen ◽  
Robert Skov ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Risk Factor ◽  
Epithelial Cells ◽  
Teichoic Acid ◽  
Nasal Colonization ◽  
Human Pathogen ◽  
Wall Teichoic Acid ◽  
Content Type ◽  
Human Nasal Epithelial Cells ◽  
Key Factor

ABSTRACT Nasal colonization by the human pathogen Staphylococcus aureus is a major risk factor for hospital- and community-acquired infections. A key factor required for nasal colonization is a cell surface-exposed zwitterionic glycopolymer, termed wall teichoic acid (WTA). However, the precise mechanisms that govern WTA-mediated nasal colonization have remained elusive. Here, we report that WTA GlcNAcylation is a pivotal requirement for WTA-dependent attachment of community-acquired methicillin-resistant S. aureus (MRSA) and emerging livestock-associated MRSA to human nasal epithelial cells, even under conditions simulating the nutrient composition and dynamic flow of nasal secretions. Depending on the S. aureus strain, WTA O-GlcNAcylation occurs in either α or β configuration, which have similar capacities to mediate attachment to human nasal epithelial cells, suggesting that many S. aureus strains maintain redundant pathways to ensure appropriate WTA glycosylation. Strikingly, a lack of WTA glycosylation significantly abrogated the ability of MRSA to colonize cotton rat nares in vivo. These results indicate that WTA glycosylation modulates S. aureus nasal colonization and may help to develop new strategies for eradicating S. aureus nasal colonization in the future. IMPORTANCE Nasal colonization by the major human pathogen Staphylococcus aureus is a risk factor for severe endogenous infections and contributes to the spread of this microbe in hospitals and the community. Here, we show that wall teichoic acid (WTA) O-GlcNAcylation is a key factor required for S. aureus nasal colonization. These data provide a mechanistic explanation for the capacity of WTA to modulate S. aureus nasal colonization and may stimulate research activities to establish valuable strategies to eradicate S. aureus nasal colonization in high-risk hospitalized patients and in the general community.

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