Zinc Oxide Nanoparticles Exhibit Favorable Properties to Promote Tissue Integration of Biomaterials

Due to the demographic change, medicine faces a growing demand for tissue engineering solutions and implants. Often, satisfying tissue regeneration is difficult to achieve especially when co-morbidities hamper the healing process. As a novel strategy, we propose the incorporation of zinc oxide nanoparticles (ZnO NPs) into biomaterials to improve tissue regeneration. Due to their wide range of biocompatibility and their antibacterial properties, ZnO NPs are already discussed for different medical applications. As there are versatile possibilities of modifying their form, size, and function, they are becoming increasingly attractive for tissue engineering. In our study, in addition to antibacterial effects of ZnO NPs, we show for the first time that ZnO NPs can foster the metabolic activity of fibroblasts as well as endothelial cells, both cell types being crucial for successful implant integration. With the gelatin sponge method performed on the chicken embryo’s chorioallantoic membrane (CAM), we furthermore confirmed the high biocompatibility of ZnO NPs. In summary, we found ZnO NPs to have very favorable properties for the modification of biomaterials. Here, incorporation of ZnO NPs could help to guide the tissue reaction and promote complication-free healing.

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Mechanistic Study of Antibacterial Properties of Chemically Synthesize Zinc Oxide Nanoparticles

Advanced Nano Research ◽

10.21467/anr.2.1.42-52 ◽

2019 ◽

Vol 2 (1) ◽

pp. 42-52

Author(s):

Abdur Rehman ◽

Saira Ahmad ◽

Abdul Mateen ◽

Huma Qamar ◽

Mudaber Ahmad Mubashar ◽

...

Keyword(s):

Zinc Oxide ◽

Zno Nanoparticles ◽

Bacterial Strain ◽

Zinc Oxide Nanoparticles ◽

Sol Gel ◽

Antibacterial Properties ◽

Mechanistic Study ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Wide Range

Nanotechnology is the science, engineering and technology conducted at the scale that ranges between 1-100 nanometers. For the bio-application, evolution of nanotechnology is creating the concern of scientists towards the synthesis of nanoparticles. The nanoparticles have unique characteristics as compare to bulk materials. Zinc oxide (ZnO) is a matchless semiconductor and it has been under investigation due to its wide range of applications in various areas like biomedical, electronics, material science and optics. In the present work synthesis of ZnO nanoparticles was carried out by using simple chemical approach, Sol-gel method for being effective and inexpensive, by employing zinc acetate dehydrate Zn (CH3CO2)2.2H2O as a precursor and sodium hydroxide (NaOH) starch as a constant agent. The structural properties of resultant zinc oxide nanoparticles were investigated by X-ray diffraction (XRD) technique. The XRD data confirmed the hexagonal wurtzite structure of ZnO powder confirmed by JCPDS 36-1451 data. Particles size was calculated by Scherrer formula and calculated size was 30.14 nm. These nanoparticles were investigated for inhibition zone of bacterial strain Escherichia coli, a gram-negative microbe, at various concentrations of ZnO nanoparticles. Zinc oxide nanoparticles were very proficient for inhibition of growth of bacterial strain E. coli. The mechanism of ZnO NPs for antibacterial activity is release of reactive oxygen species which not only hydrolyze cell wall but cell membrane and cellular components as well providing a potential bactericidal effect.

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Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

Nanomaterials ◽

10.3390/nano10061066 ◽

2020 ◽

Vol 10 (6) ◽

pp. 1066 ◽

Cited By ~ 2

Author(s):

Ľudmila Balážová ◽

Matej Baláž ◽

Petr Babula

Keyword(s):

Oxidative Stress ◽

Cell Death ◽

Zinc Oxide ◽

Programmed Cell Death ◽

Zinc Oxide Nanoparticles ◽

Cell Model ◽

Membrane Integrity ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Wide Range

Nanomaterials, including zinc oxide nanoparticles (ZnO NPs), have a great application potential in many fields, such as medicine, the textile industry, electronics, and cosmetics. Their impact on the environment must be carefully investigated and specified due to their wide range of application. However, the amount of data on possible negative effects of ZnO NPs on plants at the cellular level are still insufficient. Thus, we focused on the effect of ZnO NPs on tobacco BY-2 cells, i.e., a widely accepted plant cell model. Adverse effects of ZnO NPs on both growth and biochemical parameters were observed. In addition, reactive oxygen and nitrogen species visualizations confirmed that ZnO NPs may induce oxidative stress. All these changes were associated with the lipid peroxidation and changes in the plasma membrane integrity, which together with endoplasmatic reticulum and mitochondrial dysfunction led to autophagy and programmed cell death. The present study demonstrates that the phytotoxic effect of ZnO NPs on the BY-2 cells is very complex and needs further investigation.

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The Role of Apoptosis Pathway in the Cytotoxicity Induced by Fresh and Aged Zinc Oxide Nanoparticles

Nanoscale Research Letters ◽

10.1186/s11671-021-03587-y ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Juan Wang ◽

Lei Wang ◽

Wenting Zhao ◽

Na Yu ◽

Meiling Cheng ◽

...

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Mrna Level ◽

Signal Pathways ◽

Oxide Nanoparticles ◽

Sequencing Data ◽

Zno Nps ◽

Physicochemical Transformation ◽

Apoptosis Pathway ◽

Wide Range

AbstractZinc oxide nanoparticles (ZnO NPs) are used in a wide range of applications including industry, commercial products and medicine field. Numerous mechanistic studies for ZnO NPs’ toxicity were performed on pristine (fresh) NPs. However, the cytotoxicity induced by the transformed (aged) ZnO NPs and the underlying mechanisms remain unclear. Here, we observed the physicochemical transformation of ZnO NPs underwent over time, followed by evaluating the cytotoxicity of fresh and aged NPs. We found that fresh ZnO NPs induced higher apoptosis level than their aged counterparts. Accordingly, RNA sequencing data from aged ZnO NP-treated human–hamster hybrid (AL) cells showed that p53, PI3k–Akt, FoXO, Glutathione, ErbB, HIF-1, Oxytocin and Jak-STAT signaling pathways were enriched but no apoptosis pathway. Quantitative PCR results revealed the significantly higher mRNA level of IL1B and CD69 in fresh NP-treated groups compared to that of aged ZnO NP- and zinc chloride-treated groups. The above results indicated that the lower cytotoxicity of aged ZnO NPs is partially attributed to their reduced potency in inducing apoptosis. The transcriptional regulation of multiple signal pathways activated by aged NPs may help to build the cellular homeostasis. Taken together, our findings highlight the influence of aging (environmental transformation) process of ZnO NPs on their toxicities and biological consequences.

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Antibacterial Potential of Biosynthesized Zinc Oxide Nanoparticles against Poultry-Associated Foodborne Pathogens: An In Vitro Study

Animals ◽

10.3390/ani11072093 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2093

Author(s):

Hidayat Mohd Yusof ◽

Nor’Aini Abdul Rahman ◽

Rosfarizan Mohamad ◽

Uswatun Hasanah Zaidan ◽

Anjas Asmara Samsudin

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Foodborne Pathogens ◽

Zinc Oxide Nanoparticles ◽

Resistant Bacteria ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Wide Range ◽

Antibacterial Potential

Since the emergence of multidrug-resistant bacteria in the poultry industry is currently a serious threat, there is an urgent need to develop a more efficient and alternative antibacterial substance. Zinc oxide nanoparticles (ZnO NPs) have exhibited antibacterial efficacy against a wide range of microorganisms. Although the in vitro antibacterial activity of ZnO NPs has been studied, little is known about the antibacterial mechanisms of ZnO NPs against poultry-associated foodborne pathogens. In the present study, ZnO NPs were successfully synthesized using Lactobacillus plantarum TA4, characterized, and their antibacterial potential against common avian pathogens (Salmonella spp., Escherichia coli, and Staphylococcus aureus) was investigated. Confirmation of ZnO NPs by UV-Visual spectroscopy showed an absorption band center at 360 nm. Morphologically, the synthesized ZnO NPs were oval with an average particle size of 29.7 nm. Based on the dissolution study of Zn2+, ZnO NPs released more ions than their bulk counterparts. Results from the agar well diffusion assay indicated that ZnO NPs effectively inhibited the growth of the three poultry-associated foodborne pathogens. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were assessed using various concentrations of ZnO NPs, which resulted in excellent antibacterial activity as compared to their bulkier counterparts. S. aureus was more susceptible to ZnO NPs compared to the other tested bacteria. Furthermore, the ZnO NPs demonstrated substantial biofilm inhibition and eradication. The formation of reactive oxygen species (ROS) and cellular material leakage was quantified to determine the underlying antibacterial mechanisms, whereas a scanning electron microscope (SEM) was used to examine the morphological changes of tested bacteria treated with ZnO NPs. The findings suggested that ROS-induced oxidative stress caused membrane damage and bacterial cell death. Overall, the results demonstrated that ZnO NPs could be developed as an alternative antibiotic in poultry production and revealed new possibilities in combating pathogenic microorganisms.

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Green Synthesis, Characterization of Zinc Oxide Nanoparticles, and Examination of Properties for Dye-Sensitive Solar Cells Using Various Vegetable Extracts

Journal of Nanomaterials ◽

10.1155/2021/3941923 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Anatol Degefa ◽

Bulcha Bekele ◽

Leta Tesfaye Jule ◽

Boka Fikadu ◽

Shanmugam Ramaswamy ◽

...

Keyword(s):

Zinc Oxide ◽

Solar Cell ◽

Zinc Oxide Nanoparticles ◽

Well Being ◽

Oxide Semiconductor ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Prepared Nanoparticles ◽

Vis Spectroscopy ◽

Wide Range

The production of zinc oxide nanoparticles (ZnO NPs) utilizing different vegetable extracts (onion, cabbage, carrot, and tomato) was performed in this research owing to its excellency over other methods of synthesis, namely, simplicity, environmental friendliness, and the elimination of harmful compounds. Fresh extracted onion, cabbage, carrot, and tomato of ZnO NPs are characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and UV-visible spectroscopy. FTIR findings demonstrate that the prepared nanoparticles were observed in the spectrum of 626 cm-1–1219 cm-1 with some other functional groups. Wurtzite hexagonal structure of the prepared ZnO NPs was observed from XRD results. In addition, the prepared nanoparticles were failed into nanoscales (17 nm, 18 nm, 24 nm, and 15 nm) calculated from Scherrer’s equation. Nearly spherical shapes were seen from SEM image for onion and tomato extraction while rod and tube for carrot and cabbage, respectively. Two broad peaks were observed from UV-vis spectroscopy results for each extract. The presence of a wide range of energy bandgaps in the region of 3-4 eV was detected, indicating that ZnO NP material can be employed in metal oxide semiconductor-based systems. The dye-sensitive solar cell based on ZnO NPs has been successfully synthesized, and the efficiency of the device has been evaluated by measuring the current density-voltage behaviour under the presence of artificial sunshine. The increased effectiveness of the manufactured dye-sensitive solar cell is attributable to a large improvement in dye molecular adsorption onto the surface of ZnO NPs. Thus, the usage of the green produced ZnO NPs with creating dye sensitivity solar cell is a simple and viable way for the well-being of our future.

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Aged Zinc Oxide Nanoparticles Did Not Induce Cytotoxicity Through Apoptosis Signaling Pathway as Fresh NPs

10.21203/rs.3.rs-132318/v1 ◽

2020 ◽

Author(s):

Juan Wang ◽

Lei Wang ◽

Wenting Zhao ◽

Meiling Cheng ◽

Mingqin Su ◽

...

Keyword(s):

Zinc Oxide ◽

Signaling Pathway ◽

Zinc Oxide Nanoparticles ◽

Mrna Level ◽

Oxide Nanoparticles ◽

Sequencing Data ◽

Zno Nps ◽

Physicochemical Transformation ◽

Apoptosis Pathway ◽

Wide Range

Abstract Zinc oxide nanoparticles (ZnO NPs) are being used in a wide range of applications including industry, commercial products and medicine field. Numerous mechanistic studies for ZnO NPs’ toxicity are performed on pristine (fresh) NPs. However, the cytotoxicity induced by the transformed (aged) ZnO NPs and the underlying mechanisms remain unclear. Here, we firstly confirmed the physicochemical transformation of ZnO NPs underwent over time and compared the cytotoxicity induced by fresh and aged NPs. Then, we found that fresh NPs induced higher apoptosis levelthan aged NPs. Accordingly, RNA sequencing data from aged ZnO NP-treated human-hamster hybrid (AL) cells showed that p53, PI3k-Akt, FoXO, Glutathione, ErbB, HIF-1, Oxytocin and Jak-STAT signaling pathway were enriched but no apoptosis pathway. Quantitative PCR results confirmed the significantly higher mRNA level of IL1B and CD69 in fresh NP-treated groups compared to that of aged ZnO NP- and zinc chloride-treated groups. Our data indicated that the lower cytotoxicity of aged ZnO NPs is closely related to the low level of apoptosis induced by it and that the transcriptional regulation of the multiple pathways activated by aged NPs helps to build the cellular homeostasis. Our results highlight the aging (environmental transformation) process to the toxicity and safety assessment of ZnO NPs.

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Evaluation of the Effect of Nanoparticles Zinc Oxide/Camellia sinensis Complex on the Kidney of Rats Treated with Monosodium Glutamate: Antioxidant and Histological Approaches

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190522075928 ◽

2019 ◽

Vol 20 (7) ◽

pp. 542-550 ◽

Cited By ~ 4

Author(s):

Nahla S. El-Shenawy ◽

Reham Z. Hamza ◽

Fawziah A. Al-Salmi ◽

Rasha A. Al-Eisa

Keyword(s):

Zinc Oxide ◽

Camellia Sinensis ◽

Zinc Oxide Nanoparticles ◽

Morphological Changes ◽

Monosodium Glutamate ◽

Oxide Nanoparticles ◽

High Dose ◽

Enzymatic Antioxidants ◽

Zno Nps ◽

Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.

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Zinc oxide nanoparticles help to enhance plant growth and alleviate abiotic stress: A review

Current Protein and Peptide Science ◽

10.2174/1389203721666201016144848 ◽

2020 ◽

Vol 21 ◽

Author(s):

Mohammad Faizan ◽

Fangyuan Yu ◽

Chen Chen ◽

Ahmad Faraz ◽

Shamsul Hayat

Keyword(s):

Zinc Oxide ◽

Plant Growth ◽

Abiotic Stresses ◽

Zinc Oxide Nanoparticles ◽

Growth And Yield ◽

Oxide Nanoparticles ◽

Main Concern ◽

Negative Effects ◽

Zno Nps ◽

Agricultural Yield

: Abiotic stresses arising from atmosphere change belie plant growth and yield, leading to food reduction. The cultivation of a large number of crops in the contaminated environment is a main concern of environmentalists in the present time. To get food safety, a highly developed nanotechnology is a useful tool for promoting food production and assuring sustainability. Nanotechnology helps to better production in agriculture by promoting the efficiency of inputs and reducing relevant losses. This review examines the research performed in the past to show how zinc oxide nanoparticles (ZnO-NPs) are influencing the negative effects of abiotic stresses. Application of ZnO-NPs is one of the most effectual options for considerable enhancement of agricultural yield globally under stressful conditions. ZnO-NPs can transform the agricultural and food industry with the help of several innovative tools in reversing oxidative stress symptoms induced by abiotic stresses. In addition, the effect of ZnO-NPs on physiological, biochemical, and antioxidative activities in various plants have also been examined properly. This review summarizes the current understanding and the future possibilities of plant-ZnO-NPs research.

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Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽

10.3390/nano11030713 ◽

2021 ◽

Vol 11 (3) ◽

pp. 713

Author(s):

Nina Melnikova ◽

Alexander Knyazev ◽

Viktor Nikolskiy ◽

Peter Peretyagin ◽

Kseniia Belyaeva ◽

...

Keyword(s):

Wound Healing ◽

Zinc Oxide ◽

Bacterial Cellulose ◽

Zinc Oxide Nanoparticles ◽

Wound Dressings ◽

Oxide Nanoparticles ◽

Burn Wound ◽

Zno Nps ◽

Wound Model ◽

And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

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