scholarly journals Integrin α6β4 in Colorectal Cancer: Expression, Regulation, Functional Alterations and Use as a Biomarker

Cancers ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 41 ◽  
Author(s):  
Jean-François Beaulieu
Keyword(s):  
Colorectal Cancer ◽  
Point Of View ◽  
Molecular Forms ◽  
Cell Behaviour ◽  
Non Invasive ◽  
Laminin Receptors ◽  
Integrin Α6β4 ◽  
Functional Point ◽  
Clinical Potential

Integrin α6β4 is one of the main laminin receptors and is primarily expressed by epithelial cells as an active component of hemidesmosomes. In this article, after a brief summary about integrins in the gut epithelium in general, I review the knowledge and clinical potential of this receptor in human colorectal cancer (CRC) cells. Most CRC cells overexpress both α6 and β4 subunits, in situ in primary tumours as well as in established CRC cell lines. The mechanisms that lead to overexpression have not yet been elucidated but clearly involve specific transcription factors such as MYC. From a functional point of view, one key element affecting CRC cell behaviour is the relocalization of α6β4 to the actin cytoskeleton, favouring a more migratory and anoikis-resistant phenotype. Another major element is its expression under various molecular forms that have the distinct ability to interact with ligands (α6β4 ± ctd) or to promote pro- or anti-proliferative properties (α6Aβ4 vs. α6Bβ4). The integrin α6β4 is thus involved in most steps susceptible to participation with CRC progression. The potential clinical significance of this integrin has begun to be investigated and recent studies have shown that ITGA6 and ITGB4 can be useful biomarkers for CRC early detection in a non-invasive assay and as a prognostic factor, respectively.

scholarly journals Centering and Shifting of Centrosomes in Cells

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1351 ◽  
Author(s):  
Anton V. Burakov ◽  
Elena S. Nadezhdina
Keyword(s):  
Primary Cilia ◽  
Molecular Mechanisms ◽  
Apical Cell ◽  
Cell Polarization ◽  
Point Of View ◽  
Cell Cortex ◽  
External Cues ◽  
Intracellular Vesicles ◽  
Functional Point

Centrosomes have a nonrandom localization in the cells: either they occupy the centroid of the zone free of the actomyosin cortex or they are shifted to the edge of the cell, where their presence is justified from a functional point of view, for example, to organize additional microtubules or primary cilia. This review discusses centrosome placement options in cultured and in situ cells. It has been proven that the central arrangement of centrosomes is due mainly to the pulling microtubules forces developed by dynein located on the cell cortex and intracellular vesicles. The pushing forces from dynamic microtubules and actomyosin also contribute, although the molecular mechanisms of their action have not yet been elucidated. Centrosomal displacement is caused by external cues, depending on signaling, and is drawn through the redistribution of dynein, the asymmetrization of microtubules through the capture of their plus ends, and the redistribution of actomyosin, which, in turn, is associated with basal-apical cell polarization.

scholarly journals Circulating microRNAs as Promising Biomarkers in Colorectal Cancer

Cancers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 898 ◽  
Author(s):  
Óscar Rapado-González ◽  
Ana Álvarez-Castro ◽  
Rafael López-López ◽  
José Iglesias-Canle ◽  
María Mercedes Suárez-Cunqueiro ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Circulating Mirnas ◽  
Therapeutic Approaches ◽  
Clinical Context ◽  
Circulating Micrornas ◽  
Non Invasive ◽  
Promising Tool ◽  
Clinical Potential ◽  
Late Stages ◽  
Selection Of

Colorectal cancer (CRC) is one of the most common cancers and a leading cause of cancer-related deaths worldwide. Despite numerous advances in therapeutic approaches, this cancer has a poor prognosis when it is diagnosed at late stages. Therefore, the scientific effort is nowadays directed towards the development of new non-invasive and dynamic biomarkers to improve the survival expectancy of CRC patients. In this sense, deregulated expression of many miRNAs has been shown to play an important role for CRC carcinogenesis and dissemination. Noticeably, an increasing number of studies highlight that circulating miRNAs, including those traveling inside exosomes or those released by tumor cells into circulation, constitute a promising tool for early detection, prognosis and therapy selection of CRC. Therefore, in this review we focus on the clinical potential of blood circulating miRNAs as emerging biomarkers with high value to improve the clinical management of CRC patients, providing a deep and complete perspective of the realities and challenges to translate these biomarkers to the clinical context.

scholarly journals Moisture Damage in Ancient Masonry: A Multidisciplinary Approach for In Situ Diagnostics

Minerals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 406
Author(s):  
Noemi Proietti ◽  
Paola Calicchia ◽  
Francesco Colao ◽  
Sara De Simone ◽  
Valeria Di Tullio ◽  
...  
Keyword(s):  
Holistic Approach ◽  
Point Of View ◽  
Moisture Distribution ◽  
Non Invasive ◽  
Unilateral Nmr ◽  
Moisture Effects ◽  
Wide Range ◽  
Non Destructive

San Nicola in Carcere, one of the minor basilicas in the historical center of Rome, was the location of a wide investigation campaign of the water-related deterioration causes, present in the lower sector of the apse and adjacent pillars, affected by water infiltrations, mould and salt efflorescence. The results obtained identify the presence of water content and related effects mainly on the sides of the apsidal wall. This work focuses on the use of five Non-Destructive Techniques (NDT) and intends to show the gains obtained by integrating widely interdisciplinary methods, namely the Infrared Thermography (IRT), the Unilateral Nuclear Magnetic Resonance (Unilateral NMR), the Acoustic Tomography (AT), the Acoustic Imaging (AI) and the Laser-Induced Fluorescence (LIF). All the techniques contribute to the rapid, non-invasive and early identification of the moisture distribution, while some of them (LIF and AI) also address the determination of some moisture effects. The integrated use of different techniques helps to take the multidisciplinary point of view necessary to formulate an effective restoration intervention based on a sound scientific rationale; nonetheless, it allows to experiment a holistic approach, verifying the potential of a wide range of NDTs available within the context of a restoration yard.

scholarly journals ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer

2020 ◽  
Vol 66 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Xue-Qin Shang ◽  
Kui-Liang Liu ◽  
Qian Li ◽  
Yue-Qiong Lao ◽  
Nan-Shan Li ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Lines ◽  
Cancer Progression ◽  
Clinical Sample ◽  
Prognostic Indicator ◽  
Clinical Samples ◽  
Tissue Samples ◽  
Non Invasive ◽  
Colorectal Cancer Progression

SUMMARY OBJECTIVE ADAMTS4 is a member of the ADAMTS4 family, which secretes proteinases. The mechanism of tumor metastasis may be correlated to its promotion of angiogenesis. It was determined whether ADAMTS4 participates in colorectal cancer progression. Methods The expression in clinical samples and CRC cell lines was investigated. Using immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and RT-PCR, the expression of ADAMTS4 was determined in colorectal tumors of different cancer stages and anatomic sites, and in three cell lines of different aggressiveness. Results The overexpression of ADAMTS4 was observed in tissue samples by IHC, and this was mainly located in the cytoplasm, as detected by FISH. The qRT-PCR and western blot analyses further supported the clinical sample findings. Conclusion The present data support the notion that the overexpression of ADAMTS4 in CRC might be useful as a non-invasive biomarker for detecting CRC in patients.

In situ TEM studies of irradiation effects for materials improvement

1991 ◽  
Vol 49 ◽  
pp. 452-453
Author(s):  
Charles W. Allen
Keyword(s):  
Nuclear Reactor ◽  
Austenitic Stainless Steels ◽  
High Energy ◽  
Point Of View ◽  
In Situ Tem ◽  
Irradiation Effects ◽  
Tem Analysis ◽  
Radiation Induced ◽  
Analytical Tools

Irradiation effects studies employing TEMs as analytical tools have been conducted for almost as many years as materials people have done TEM, motivated largely by materials needs for nuclear reactor development. Such studies have focussed on the behavior both of nuclear fuels and of materials for other reactor components which are subjected to radiation-induced degradation. Especially in the 1950s and 60s, post-irradiation TEM analysis may have been coupled to in situ (in reactor or in pile) experiments (e.g., irradiation-induced creep experiments of austenitic stainless steels). Although necessary from a technological point of view, such experiments are difficult to instrument (measure strain dynamically, e.g.) and control (temperature, e.g.) and require months or even years to perform in a nuclear reactor or in a spallation neutron source. Consequently, methods were sought for simulation of neutroninduced radiation damage of materials, the simulations employing other forms of radiation; in the case of metals and alloys, high energy electrons and high energy ions.

Circulating matrix metaloproteinases as biomarkers in colorectalcancer

2020 ◽  
Vol 99 (9) ◽  
Keyword(s):  
Colorectal Cancer ◽  
Tumor Markers ◽  
Point Of View ◽  
Tissue Inhibitors ◽  
Natural Tissue

The aim of this research is to offer comprehensive point of view related to perspective tumor markers called matrix metaloproteinases and their natural tissue inhibitors. Those markers are potentially useable mainly in postoperative follow-up in patients with colorectal cancer.

Efficiency of diagnosis and treatment non-invasive and microinvasive sgamous cell cervical cancer

2020 ◽  
pp. 71-74
Author(s):  
M.M. Melnyk ◽  
◽  
S.V. Nespradko ◽  
I.V. Goncharuk ◽  
M.V. Marchenko ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Key Words ◽  
National Cancer Institute ◽  
Reproductive Function ◽  
Diagnosis And Treatment ◽  
Non Invasive ◽  
Corpus Uteri ◽  
Complex Program ◽  
Early Cervical Cancer

The objective: analyse the effectiveness of diagnosis and treatment for early cervical cancer. Materials and methods. Analysed 107 cases of women’s disease on CIN ІІІ, cancer in situ, they were on treatment in National cancer institute and Kyiv dictrict cancer dispensary from 2010 till 2015 years. Results. Diagnosed percent relapse CIN ІІІ, cancer in situ contain 4.57% uninvasive and invasive form – 0.94%. Conclusion. According diagnostic CIN ІІ and CIN ІІІ is recommended to do treatment conization and dynamic dispensary observation. Are making complex program of infection HPV16, 18. In appering of margins resection some elements of tumor after wider conization by forms of cancer in situ. Many of expansive burns in cervical glands, in making of reproductive function, going disease (nodel leiomyoma of corpus uteri etc). In perspective is accept the notion of looking after and screening research of considering infection HPV16, 18 on CIN І, CIN ІІ. Key words: cervical cancer, сancer in situ, CIN І–ІІІ, diagnostic, treatment, conization.

Fecal microRNAs as non-invasive biomarkers for the detection of colorectal cancer: a systematic review

2019 ◽  
Vol 31 (1) ◽  
Author(s):  
Antonio Francavilla ◽  
Sonia Tarallo ◽  
Barbara Pardini ◽  
Alessio Naccarati
Keyword(s):  
Colorectal Cancer ◽  
Systematic Review ◽  
Non Invasive

Dental Inclusion of Canine and Wisdom Tooth in Orthodontics Chemical Necroses

2018 ◽  
Vol 69 (8) ◽  
pp. 2191-2196
Author(s):  
Cristian Constantin Budacu ◽  
Nicoleta Ioanid ◽  
Cristian Romanec ◽  
Mihail Balan ◽  
Liliana Lacramioara Pavel ◽  
...  
Keyword(s):  
Third Molar ◽  
Point Of View ◽  
Separate Entity ◽  
Wisdom Tooth ◽  
Facial Aesthetics ◽  
The Third ◽  
Edentulous Mandible ◽  
Canine Guidance ◽  
Upper Jaw ◽  
Functional Point

Canine plays an important role in the dento-maxillary system. From a functional point of view, it provides the canine guidance, by positioning it in the frontal area, has a role in facial aesthetics. It plays an important prosthetic role by having the longest root and one of the longest arcade teeth. Three molars represent the last teeth that erupt in the arches both in the jaw and in the mandible, which is why they remain the most frequently included.Canine incidence is quite common following the wisdom tooth. It can be unilateral or bilateral and is more common in the upper jaw. The canine may remain included at the vestibular, palatal or between the two bones. A separate entity is the incision of the canine in the edentulous mandible or jaw. The study included 213 cases with dento-alveolar pathology, of which 128 patients were selected with dental inclusion. Our study reports that the first three molars are frequent, followed by the canine as opposed to other studies conducted by Guzduz K in 2011 and Fardi A of the same year bringing the canines first (Fardi, Guzduz). Some studies attribute the first place to the superior canine in terms of frequency, but they are abstracted from the molar three inclusion that they consider as most frequently (Compoy). The most common tooth in inclusion is the third molar (lower and upper) followed by the upper canine; the most commonly affected are women for both canine and molar.

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