scholarly journals Enhancing Protein Crystallization under a Magnetic Field

Crystals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 821
Author(s):  
Sun Young Ryu ◽  
In Hwan Oh ◽  
Sang Jin Cho ◽  
Shin Ae Kim ◽  
Hyun Kyu Song

High-quality crystals are essential to ensure high-resolution structural information. Protein crystals are controlled by many factors, such as pH, temperature, and the ion concentration of crystalline solutions. We previously reported the development of a device dedicated to protein crystallization. In the current study, we have further modified and improved our device. Exposure to external magnetic field leads to alignment of the crystal toward a preferred direction depending on the magnetization energy. Each material has different magnetic susceptibilities depending on the individual direction of their unit crystal cells. One of the strategies to acquire a large crystal entails controlling the nucleation rate. Furthermore, exposure of a crystal to a magnetic field may lead to new morphologies by affecting the crystal volume, shape, and quality.

2020 ◽  
Vol 9 (1-2) ◽  
pp. 101-110 ◽  
Author(s):  
Daniel Holder ◽  
Artur Leis ◽  
Matthias Buser ◽  
Rudolf Weber ◽  
Thomas Graf

AbstractAdditively manufactured parts typically deviate to some extent from the targeted net shape and exhibit high surface roughness due to the size of the powder grains that determines the minimum thickness of the individual slices and due to partially molten powder grains adhering on the surface. Optical coherence tomography (OCT)-based measurements and closed-loop controlled ablation with ultrashort laser pulses were utilized for the precise positioning of the LPBF-generated aluminum parts and for post-processing by selective laser ablation of the excessive material. As a result, high-quality net shape geometries were achieved with surface roughness, and deviation from the targeted net shape geometry reduced by 67% and 63%, respectively.


2007 ◽  
Vol 21 (01) ◽  
pp. 127-132
Author(s):  
T. R. YANG ◽  
G. ILONCA ◽  
V. TOMA ◽  
P. BALINT ◽  
M. BODEA

The scaling behavior of the effective activation energy of high-quality epitaxial c-oriented Bi 2 Sr 2 Ca ( Cu 1-x Co x)2 O d thin films with 0≤x ≤0.025 has been studied as a function of temperature and magnetic field. For all samples, the effective activation energy scales as U(T, μoH)=Uo(1-T/T c )mHn with exponent m=1.25±0.03, n=-1/2 and the field scaling 1/μoH and -UμoH for thick films and ultra thin films, respectively. The results are discussed taking into account of the influence of the Co substitution with a model in which U(T, H) arises from plastic deformations of the viscous flux liquid above the vortex-glass transition temperature.


2021 ◽  
pp. 73-78
Author(s):  
Svetlana Vladimirovna Kropotova

The purpose of the study is to improve the adaptation system for personnel in a multidisciplinary hospital. Results: the problems of organizing the adaptation process were identified, the methods of analysis and assessment of the management of the adaptation process in a medical organization were adapted, the effectiveness of the existing system of adaptation of medical workers in the organization was assessed. Conclusion: the study proved the need for a more complete and high-quality adaptation process; the process is not static, a creative approach is needed, taking into account the characteristics of the organization (team) and the individual abilities of a specialist; to improve the organization, for the effective adaptation of medical personnel, it is necessary to develop the institution of mentoring.


2018 ◽  
Vol 857 ◽  
pp. 398-448 ◽  
Author(s):  
Chamkor Singh ◽  
Arup K. Das ◽  
Prasanta K. Das

The central theme of this work is that a stable levitation of a denser non-magnetizable liquid droplet, against gravity, inside a relatively lighter ferrofluid – a system barely considered in ferrohydrodynamics – is possible, and exhibits unique interfacial features; the stability of the levitation trajectory, however, is subject to an appropriate magnetic field modulation. We explore the shapes and the temporal dynamics of a plane non-magnetizable droplet levitating inside a ferrofluid against gravity due to a spatially complex, but systematically generated, magnetic field in two dimensions. The coupled set of Maxwell’s magnetostatic equations and the flow dynamic equations is integrated computationally, utilizing a conservative finite-volume-based second-order pressure projection algorithm combined with the front-tracking algorithm for the advection of the interface of the droplet. The dynamics of the droplet is studied under both the constant ferrofluid magnetic permeability assumption as well as for more realistic field-dependent permeability described by Langevin’s nonlinear magnetization model. Due to the non-homogeneous nature of the magnetic field, unique shapes of the droplet during its levitation, and at its steady state, are realized. The complete spatio-temporal response of the droplet is a function of the Laplace number $La$ , the magnetic Laplace number $La_{m}$ and the Galilei number $Ga$ ; through detailed simulations we separate out the individual roles played by these non-dimensional parameters. The effect of the viscosity ratio, the stability of the levitation path and the possibility of existence of multiple stable equilibrium states is investigated. We find, for certain conditions on the viscosity ratio, that there can be developments of cusps and singularities at the droplet surface; we also observe this phenomenon experimentally and compare with the simulations. Our simulations closely replicate the singular projection on the surface of the levitating droplet. Finally, we present a dynamical model for the vertical trajectory of the droplet. This model reveals a condition for the onset of levitation and the relation for the equilibrium levitation height. The linearization of the model around the steady state captures that the nature of the equilibrium point goes under a transition from being a spiral to a node depending upon the control parameters, which essentially means that the temporal route to the equilibrium can be either monotonic or undulating. The analytical model for the droplet trajectory is in close agreement with the detailed simulations.


2013 ◽  
Vol 21 (1) ◽  
pp. 203-208 ◽  
Author(s):  
Yannick G. Spill ◽  
Seung Joong Kim ◽  
Dina Schneidman-Duhovny ◽  
Daniel Russel ◽  
Ben Webb ◽  
...  

Small-angle X-ray scattering (SAXS) is an experimental technique that allows structural information on biomolecules in solution to be gathered. High-quality SAXS profiles have typically been obtained by manual merging of scattering profiles from different concentrations and exposure times. This procedure is very subjective and results vary from user to user. Up to now, no robust automatic procedure has been published to perform this step, preventing the application of SAXS to high-throughput projects. Here,SAXS Merge, a fully automated statistical method for merging SAXS profiles using Gaussian processes, is presented. This method requires only the buffer-subtracted SAXS profiles in a specific order. At the heart of its formulation is non-linear interpolation using Gaussian processes, which provides a statement of the problem that accounts for correlation in the data.


2004 ◽  
Vol 44 (2) ◽  
pp. 58-63
Author(s):  
Gen SAZAKI ◽  
Shin-ichiro YANAGIYA ◽  
Yoshihisa SUZUKI ◽  
Satoru MIYASHITA ◽  
Takao SATO ◽  
...  

2020 ◽  
Vol 7 (1) ◽  
pp. 925
Author(s):  
Teresa Quilhó ◽  
Fernanda Bessa ◽  
Ana Isabel Ribeiro-Barros ◽  
Natasha Ribeiro

Pterocarpus angolensis and Terminalia sericea are two African species with medicinal potential. Despite the importance of their bark as a powerful astringent to treat various diseases it is poor described. In order to provide referential information for correct identification and standardization of the plant material, bark samples from each two species were collected and analyzed under light and electron microscopy. Some important anatomical features to identification were: the sclerenchyma tissue mostly in form of fibre-sclereids and the large secretory cells arranged in conspicuous rows or tangential bands in the conducting phloem in P. angolensis; the crystalliferous cells arranged in very regular tangential rows (druses) and the occurrence of large crystal cells near or including the tangential fibre bundles in T. sericea bark.The results obtained show that the anatomy of the bark can be used as an important subsidy in identification and standardization of the studied species contributing the scientific knowledge for more effective forms of scrutiny in preventing commercial adulteration of species.


2020 ◽  
Vol 10 (1) ◽  
pp. 44-47
Author(s):  
Jakub Kisała ◽  
Karolina Czarnacka ◽  
Mateusz Gęca ◽  
Andrzej Kociubiński

The paper presents the technology for obtaining NiFe/Ti/NiFe layer structures in MEMS technology using magnetron purge with the assumption of being used as semi-magnetic sensors. A series of samples was made on a glass substrate with a sandwich structure, where the individual layers were 100 nm NiFe, 10 nm Ti and on top again NiFe with a thickness of 100 nm. Measurements of DC resistance of the obtained structures in a constant magnetic field, which was produced by neodymium magnets and an electromagnet, were carried out. The obtained results confirm the occurrence of phenomena known as the magnetoresistance effect. The influence of the spatial arrangement of structures relative to the constant magnetic field vector was checked and proved.


Hyomen Kagaku ◽  
1999 ◽  
Vol 20 (11) ◽  
pp. 770-776
Author(s):  
Gen SAZAKI ◽  
Shin-ichiro YANAGIYA ◽  
Stephen D. DURBIN ◽  
Satoru MIYASHITA ◽  
Toshitaka NAKADA ◽  
...  

2019 ◽  
Vol 19 (2) ◽  
pp. 195-201
Author(s):  
Chris M. Hall ◽  
Magnar G. Johnsen

AbstractA hypothesis is proposed wherein changes in the Earth's magnetic field affect the migratory paths of snow buntings (Plectrophenax nivalis), and in particular from wintering grounds in the Russian/Ukrainian steppes to breeding grounds on Svalbard and with a typical stopover in Finnmark in northern Norway. If one were to assume ignorance of the secular movement of the magnetic north pole approximately 1500 km northwards between 1908 and 2020, the magnetoreceptor contribution to snow buntings' navigation would result in winter-to-summer migratory paths progressively further to the East. In turn, this could be a contributing factor to declining populations in Finnmark and favouring a more frequent flightpath over the Kola Peninsula. On the other hand, short-term perturbations in the magnetic field (i.e. induced by solar activity) and therefore existing for a relatively small proportion of the flight time (if at all) for the individual migrations legs seem unlikely to influence the stopover locations significantly. Even so, these space-weather induced variations cannot be disregarded, particularly for success in reaching Svalbard.


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