Synthesis, Characterization, Single-Crystal X-ray Structure and Biological Activities of [(Z)-N′-(4-Methoxybenzylidene)benzohydrazide–Nickel(II)] Complex

(Z)-N′-(4-methoxybenzylidene)benzohydrazide (HL) and its Ni(II) complex (Ni(II)-2L) were synthesized using eco-friendly protocols. The single X-ray crystal structure of Ni(II)-2L was solved. Moreover, the structural properties were evaluated using Fourier transform infrared, proton nuclear magnetic resonance, mass, and Ultraviolet/Visible spectroscopy. The diamagnetic and thermal stability were assessed using magnetic susceptibility and thermogravimetric analysis, respectively. The biological activities of both HL and Ni(II)-2L (62.5–1000 μg/mL) against Gram-positive (Staphylococcus aureus and Streptococcus pyogenes) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacterial and fungal (Candida albicans, Aspergillus niger, and Aspergillus clavatus) species were studied using the minimum inhibitory concentration (MIC) tests method in reference to Gentamycin and Nystatin standard drugs, respectively. The results revealed an affordable, environmentally friendly, and efficient synthetic method of HL using water as a green solvent. The Ni(II)-2L complex crystallized in a distorted square planar, P21/n space group, and one Ni(II) to two bidentate negatively charged ligand ratio. The analysis of biological activity revealed higher activity of the complex against S. aureus and S. pyogenes (bacteria) and A. niger and A. clavatus (fungi) compared to the ligand. However, the highest activity was at a MIC of 62.5 μg/mL for the complex against S. pyogenes and for the ligand against E. coli. Therefore, both HL and Ni(II)-2L could be promising potential antimicrobials and their selective activity could be an additional benefit of these bioactive materials.

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Preparation and disinfection properties of graphene oxide/trichloroisocyanuric acid disinfectant

Nanotechnology ◽

10.1088/1361-6528/ac3b82 ◽

2021 ◽

Author(s):

li li jiang ◽

Su Xu ◽

Haitao Yu ◽

Qi Cui ◽

Rui Cao

Keyword(s):

Electron Microscopy ◽

Graphene Oxide ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Kinetic Curve ◽

X Ray ◽

Bacterial Inhibition ◽

E Coli ◽

Blending Method ◽

Trichloroisocyanuric Acid

Abstract In this study, graphene oxide (GO) was first prepared by the modified Hummer method. Then, the GO/trichloroisocyanuric acid (TCCA) composite was prepared by loading TCCA into GO with the blending method. X-ray diffraction, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize the composite. The results showed that TCCA was successfully loaded on the surface of GO or intercalated among GO layers. Next, the antibacterial performance of the composite against Escherichia coli and Staphylococcus aureus was tested by the 96-well plate assay. A bactericidal kinetic curve, bacterial inhibition tests, and the mechanism of bacterial inhibition is discussed. The results showed that the minimum inhibitory concentration of the GO/TCCA composite (GO:TCCA ratio = 1:50) was 327.5 µg/mL against E. coli and 655 µg/mL against S. aureus. At the minimum inhibitory concentration, the inhibition rate of the GO/TCCA composite exceeded 99.46% against E. coli and 99.17% against S. aureus. The bactericidal kinetic curves indicate that the GO/TCCA composite has an excellent bactericidal effect against E. coli and S. aureus.

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Chemical Composition and Biological Activities of Metabolites from the Marine Fungi Penicillium sp. Isolated from Sediments of Co To Island, Vietnam

Molecules ◽

10.3390/molecules24213830 ◽

2019 ◽

Vol 24 (21) ◽

pp. 3830

Author(s):

Hong Le ◽

Quynh Do ◽

Mai Doan ◽

Quyen Vu ◽

Mai Nguyen ◽

...

Keyword(s):

Minimum Inhibitory Concentration ◽

Secondary Metabolites ◽

Inhibitory Concentration ◽

Biological Activities ◽

2D Nmr ◽

Inhibitory Effect ◽

Marine Microorganisms ◽

Penicillium Species ◽

E Coli ◽

Strong Inhibitory Effect

Marine microorganisms are an invaluable source of novel active secondary metabolites possessing various biological activities. In this study, the extraction and isolation of the marine sediment Penicillium species collected in Vietnam yielded ten secondary metabolites, including sporogen AO-1 (1), 3-indolecarbaldehyde (2), 2-[(5-methyl-1,4-dioxan-2-yl)methoxy]ethanol (3), 2-[(2R-hydroxypropanoyl)amino]benzamide (4), 4-hydroxybenzandehyde (5), chrysogine (6), 3-acetyl-4-hydroxycinnoline (7), acid 1H-indole-3-acetic (8), cyclo (Tyr-Trp) (9), and 2’,3’-dihydrosorbicillin (10). Their structures were identified by the analysis of 1D and 2D NMR data. Among the isolated compounds, 2-[(5-methyl-1,4-dioxan-2-yl)methoxy]ethanol (3) showed a strong inhibitory effect against Enterococcus faecalis with a minimum inhibitory concentration value of 32 µg/mL. Both 2-[(2R-hydroxypropanoyl)amino]benzamide (4) and 4-hydroxybenzandehyde (5) selectively inhibited E. coli with minimum inhibitory concentration values of 16 and 8 µg/mL, respectively. 2’,3’-Dihydrosorbicillin (10) potentially inhibited α-glucosidase activity at a concentration of 2.0 mM (66.31%).

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Synthesis and Structures of Halo-Substituted Aroylhydrazones with Antimicrobial Activity

Australian Journal of Chemistry ◽

10.1071/ch11424 ◽

2012 ◽

Vol 65 (4) ◽

pp. 343 ◽

Cited By ~ 92

Author(s):

Mei Zhang ◽

Dong-Mei Xian ◽

Hai-Hua Li ◽

Ji-Cai Zhang ◽

Zhong-Lu You

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Inhibitory Concentration ◽

Antimicrobial Activities ◽

X Ray Diffraction ◽

X Ray ◽

E Coli ◽

Diphenyl Tetrazolium Bromide

A series of new halo-substituted aroylhydrazones have been prepared and structurally characterized by elemental analysis, 1H NMR, 13C NMR, and IR spectra, and single crystal X-ray diffraction. The compounds were evaluated for their antibacterial (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas fluorescence) and antifungal (Candida albicans and Aspergillus niger) activities by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. Among the tested compounds, N′-(2-chloro-5-nitrobenzylidene)-2-fluorobenzohydrazide showed the most effective antimicrobial activity with minimum inhibitory concentration values of 0.82, 2.5, 1.7, 15.2, and 37.5 μg mL–1 against B. subtilis, S. aureus, E. coli, P. fluorescence, and C. albicans, respectively. The biological assay indicated that the presence of the electron-withdrawing groups in the aroylhydrazones improved their antimicrobial activities.

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Third-order Nonlinear Optical Properties of Copper(II)bis{2-[(4-iodophenyl)iminomethyl]-6-methoxy-phenolate}

Zeitschrift für Naturforschung B ◽

10.1515/znb-2006-1107 ◽

2006 ◽

Vol 61 (11) ◽

pp. 1355-1360 ◽

Cited By ~ 1

Author(s):

Halime G. Yaglioglu ◽

Asli Karakaş ◽

Hseyin Ünver ◽

Ayhan Elmali

Keyword(s):

Title Complex ◽

X Ray Diffraction ◽

Metal Centre ◽

Visible Spectroscopy ◽

Third Order ◽

X Ray ◽

Hartree Fock ◽

Near Ir ◽

Square Planar ◽

Uv Visible

Copper(II)bis{2-[(4-iodophenyl)iminomethyl]-6-methoxy-phenolate} has been synthesized, characterized by UV-visible spectroscopy, and its crystal structure determined by X-ray diffraction. The Cu atom is situated on a twofold axis and the geometry around the metal centre can be described as distorted square planar with a trans configuration. The absorption maxima are shorter than 450 nm, giving rise to good optical transparency in the visible and near IR. To reveal the microscopic thirdorder NLO properties, the static second hyperpolarizabilities have been evaluated by using the ab initio time-dependent Hartree-Fock (TDHF) method. According to the results, the title complex exhibits non-zero γ values, implying microscopic third-order NLO behavior.

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Host-guest interaction and properties of cucurbit[8]uril with chloramphenicol

10.3762/bxiv.2021.62.v1 ◽

2021 ◽

Author(s):

Lin Zhang ◽

Jun Zheng ◽

Guangyan Luo ◽

Xiaoyue Li ◽

Yunqian Zhang ◽

...

Keyword(s):

Inhibitory Concentration ◽

Slow Release ◽

In Vitro Release ◽

Titration Calorimetry ◽

X Ray Diffraction ◽

X Ray ◽

E Coli ◽

The Stability ◽

Vitro Release

The interaction between cucurbit[8]uril (Q[8]) and chloramphenicol (CPE) was investigated using single-crystal X-ray diffraction, spectroscopy, isothermal titration calorimetry (ITC) and UV-Vis, NMR and IR spectroscopy. The effects of Q[8] on the stability, in vitro release performance and antibacterial activity of CPE were also studied. The results showed that CPE and Q[8] formed a 1:1 inclusion complex (CPE@Q[8]) with an inclusion constant of 5.4736 × 105 L/mol. The intervention of Q[8] did not affect the stability of CPE, but obviously reduced the release rate of CPE in artificial gastric and intestinal juice; Q[8] has a slow-release effect on CPE. The antibacterial results showed that the minimum inhibitory concentration (MIC) of CPE and CPE@Q[8] toward Escherichia coli (E. coli) was 1.5 × 10–3 and 1.0 × 10–3 mol/L, respectively, and toward Staphylococcus aureus (SA), the MIC was 2.0 × 10–3 mol/L for both CPE and CPE@Q[8]. Therefore, Q[8] enhanced the inhibitory activity of CPE against E. coli.

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Synthesis, Characterization, and Crystal Structure of Pyridinium Tetrakis{1-trifluoromethyl-3-(1′-pyreno)-1,3-propanedionato}lanthanate(III) Complex

Journal of Crystallography ◽

10.1155/2014/271238 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6

Author(s):

Tetsuji Moriguchi ◽

Kenji Yoza ◽

Akihiko Tsuge

Keyword(s):

Ligand Exchange ◽

Fast Atom Bombardment ◽

Proton Nuclear Magnetic Resonance ◽

Resonance Spectroscopy ◽

X Ray Diffraction ◽

Visible Spectroscopy ◽

X Ray ◽

Mild Conditions ◽

Polycyclic Aromatic ◽

Halide Ligand

The reaction of 1-trifluoromethyl-3-(1′-pyreno)-1,3-propanedione with lanthanum(III) trichloride in the presence of pyridine leads to halide ligand exchange, yielding a novel pyridinium tetrakis{1-trifluoromethyl-3-(1′-pyreno)-1,3-propanedionato}lanthanate(III) complex with four polycyclic aromatic hydrocarbon moieties. This reaction easily proceeds under mild conditions (25°C, 24 h) producing the complex in a 90% isolated yield. The complex was characterized by infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and positive fast atom bombardment mass spectrometry, and its precise structure was determined by single crystal X-ray diffraction. Ultraviolet-visible spectroscopy, fluorescence spectroscopy, and X-ray analysis demonstrated that the four pyrene moieties were independently oriented.

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Moderations in performance, immunity, tissue architecture, and vaccine viability induced by water magnetization in broiler farms

Veterinary World ◽

10.14202/vetworld.2021.1695-1710 ◽

2021 ◽

pp. 1695-1710

Author(s):

Essam S. Soliman ◽

Rania T. Hamad ◽

Rania A. Hassan

Keyword(s):

Broiler Chickens ◽

Inhibitory Concentration ◽

Biological Activities ◽

Negative Influence ◽

Immunoglobulin M ◽

Tissue Architecture ◽

E Coli ◽

Influence Of Water ◽

Magnetic Water

Background and Aim: Water magnetization contributes to increased molecular ionization and fluidity, which improves biological activities. This study tests the influence of magnetic water on the viability of the Newcastle vaccine and the survival of Escherichia coli and Salmonella Typhimurium, as well as the influence of magnetic water in face of water impurities' challenges on performance, immunity, and tissue architecture in broiler chickens. Materials and Methods: An in vitro 96-micro-well plate minimal inhibitory concentration was utilized to test the influence of water, saline, and magnetic water on Newcastle vaccine viability and E. coli O157:H7 and S. Typhimurium survival. The 245 experimental 1-day-old female Ross® 308 broilers used in this study were divided into seven groups of 35 birds each. Broilers were provided with magnetic drinking water (13,200 gausses) for 6 h daily from the 5th day and were challenged on days 14, 21, 28, and 35 using sodium chloride (700 mg/L), calcium sulfate (80 mg/L), lead acetate (500 mg/L), yeast extract 5% (5 mg/L), diazinon (2.5 mL/L), and E. coli O157:H7 (1.6 × 109 CFU/mL). A total of 2040 samples (96 diluent-Newcastle virus vaccine mixes, 96 microbial-magnetic water mixes, 231 sera, 231 intestinal swabs, and 1386 organ samples) were collected. Results: An in vitro trial revealed highly significant (p<0.01) declines of 94.13%, 84.53%, and 10.31% in the Newcastle vaccine titer in water, saline, and magnetic water, respectively, and 54.91% and 39.89% in E. coli O157:H7 and S. Typhimurium survival, respectively, after 4 h. In all challenged groups, broilers exhibited highly significant (p<0.01) increases in performance, carcass/organs weight, immunoglobulin G, immunoglobulin M, and Lactobacillus counts; significant improvement in tissue architecture and biochemical parameters; and highly significant (p<0.01) reductions in cortisol, superoxide dismutase, and total bacterial and Enterobacteriaceae counts. Conclusion: Magnetic water could maintain vaccine viability and vaccination efficiency, reduce microbial survival, and minimize the negative influence of all induced challenges.

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Biosynthesis of Silver Nanoparticles by Aspergillus terreus: Characterization, Optimization, and Biological Activities

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.633468 ◽

2021 ◽

Vol 9 ◽

Author(s):

Walid A. Lotfy ◽

Basma M. Alkersh ◽

Soraya A. Sabry ◽

Hanan A. Ghozlan

Keyword(s):

Silver Nanoparticles ◽

Aspergillus Terreus ◽

Inhibitory Concentration ◽

Biological Activities ◽

Antagonistic Activity ◽

Metallic Silver ◽

X Ray Diffraction ◽

X Ray ◽

Transmission Electron

In this study, mycelial filtrate of Aspergillus terreus BA6 was used to reduce AgNO3 to form silver nanoparticles (AgNPs). The effect of seven independent variables on the diameter of AgNPs was studied by applying design of experiments (DOE). At optimal conditions, the diameter of AgNPs was reduced by approximately 26.7% compared to the basal culture condition and AgNO3 concentration was found to be the most significant factor affecting the diameter of AgNPs. A. terreus nano-Ag was characterized using UV-visible spectroscopy, transmission electron microscopy, energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Zeta potential. The maximum UV absorption was obtained at 420 nm and the microscopic results showed particles with narrow size distribution ranging from 7 to 23 nm. XRD pattern of AgNPs revealed four diffraction peaks of metallic silver and the EDX spectrum showed a strong signal attributed to Ag nano-crystals. AgNPs mycofabricated by A. terreus showed potent minimum inhibitory concentration (MIC) and broad minimum bactericidal/fungicidal concentration (MBC/MFC) against 12 reference microorganisms. The MIC and MBC/MFC values of AgNPs were 0.312 to 1.25 μg/ml and 0.625 to 10 μg/ml, respectively. Nevertheless, AgNPs did not demonstrate any antagonistic activity against Coxsackie B virus. The in vitro cytotoxicity of the mycosynthesized AgNPs showed significant antitumor activity against adenocarcinoma epithelial cells from human breast cancer (Mcf-7) cell line with an inhibitory concentration (IC50) of 87.5 μg/ml.

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Host–guest interaction and properties of cucurbit[8]uril with chloramphenicol

Beilstein Journal of Organic Chemistry ◽

10.3762/bjoc.17.194 ◽

2021 ◽

Vol 17 ◽

pp. 2832-2839

Author(s):

Lin Zhang ◽

Jun Zheng ◽

Guangyan Luo ◽

Xiaoyue Li ◽

Yunqian Zhang ◽

...

Keyword(s):

Inhibitory Concentration ◽

Slow Release ◽

In Vitro Release ◽

Titration Calorimetry ◽

X Ray Diffraction ◽

X Ray ◽

E Coli ◽

The Stability ◽

Vitro Release

The interaction between cucurbit[8]uril (Q[8]) and chloramphenicol (CPE) was investigated using single-crystal X-ray diffraction spectroscopy, isothermal titration calorimetry (ITC) and UV–vis, NMR and IR spectroscopy. The effects of Q[8] on the stability, in vitro release performance and antibacterial activity of CPE were also studied. The results showed that CPE and Q[8] formed a 1:1 inclusion complex (CPE@Q[8]) with an inclusion constant of 5.474 × 105 L/mol. The intervention of Q[8] did not affect the stability of CPE, but obviously reduced the release rate of CPE in artificial gastric and intestinal juice; Q[8] has a slow-release effect on CPE. The antibacterial results showed that the minimum inhibitory concentration (MIC) of CPE and CPE@Q[8] toward Escherichia coli (E. coli) was 1.5 × 10–3 and 1.0 × 10–3 mol/L, respectively, and toward Staphylococcus aureus (S. aureus), the MIC was 2.0 × 10–3 mol/L for both CPE and CPE@Q[8]. Therefore, Q[8] enhanced the inhibitory activity of CPE against E. coli.

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Magnetic β-Cyclodextrin Nanosponges for Potential Application in the Removal of the Neonicotinoid Dinotefuran from Wastewater

International Journal of Molecular Sciences ◽

10.3390/ijms21114079 ◽

2020 ◽

Vol 21 (11) ◽

pp. 4079

Author(s):

Sebastián Salazar ◽

Nicolás Yutronic ◽

Paul Jara

Keyword(s):

Electron Microscopy ◽

Proton Nuclear Magnetic Resonance ◽

Vibrating Sample Magnetometer ◽

Adsorption Efficiency ◽

Visible Spectroscopy ◽

X Ray ◽

Transmission Electron ◽

Cyclodextrin Nanosponges ◽

Aqueous Environments ◽

Uv Visible

This article describes the use of β-cyclodextrin-based carbonate nanosponges (NSs) decorated with superparamagnetic Fe3O4 nanoparticles to study and investigate the potential removal of dinotefuran (DTF) from wastewater. The NS-DTF inclusion compound was characterized by transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDS), UV-visible spectroscopy (UV-VIS), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), X-ray powder diffraction (XRPD) and proton nuclear magnetic resonance (1H-NMR). The adsorption efficiency of NSs was evaluated as function of different contact times. The results confirmed that the NSs have a favourable sorption capacity for the chosen guest, as the polymers exhibited a maximum adsorption of 4.53 × 10−3 mmol/g for DTF. We also found that magnetic NSs show good reusability as they maintain their efficiency after eight adsorption and desorption cycles. Our studies and characterization by means of SEM, TEM, EDS, vibrating sample magnetometer (VSM) and UV-VIS also show that NSs with magnetic properties are excellent tools for insecticide removal from aqueous environments.

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