scholarly journals Rice Bran Fermentation Using Lactiplantibacillus plantarum EM as a Starter and the Potential of the Fermented Rice Bran as a Functional Food

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 978
Author(s):  
Song-Hee Moon ◽  
Hae-Choon Chang
Keyword(s):  
Antimicrobial Activity ◽  
Rice Bran ◽  
Functional Food ◽  
Pathogenic Bacteria ◽  
Corn Steep Liquor ◽  
Starter Culture ◽  
Antimicrobial Activities ◽  
Cholesterol Removal ◽  
Cell Counts ◽  
Strong Antimicrobial Activity

Rice bran was fermented using a functional starter culture of Lactiplantibacillus plantarum EM, which exhibited high cholesterol removal and strong antimicrobial activity. Highest viable cell counts (9.78 log CFU/mL) and strong antimicrobial activity were obtained by fermenting 20% rice bran supplemented with 1% glucose and 3% corn steep liquor (pH 6.0) at 30 °C for 48 h. The fermented rice bran slurry was hot air-dried (55 °C, 16 h) and ground (HFRB). HFRB obtained showed effective cholesterol removal (45–68%) and antimicrobial activities (100–400 AU/mL) against foodborne pathogenic bacteria and food spoilage fungi. Phytate levels were significantly reduced during fermentation by 53% due to the phytase activity of L. plantarum EM, indicating HFRB does not present nutrient deficiency issues. In addition, fermentation significantly improved overall organoleptic quality. Our results indicate that HFRB is a promising functional food candidate. Furthermore, HFRB appears to satisfy consumer demands for a health-promoting food and environmental and legal requirements concerning the re-utilization of biological byproducts.

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

scholarly journals Characterization and Antimicrobial Activities of a Metabolite from a New Streptomyces Species from Bangladeshi Soil

2009 ◽  
Vol 2 (1) ◽  
pp. 178-185
Author(s):  
M. Akhand ◽  
M. A. A. Al-Bari ◽  
M. A. Islam ◽  
Proma Khondkar
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Assay Method ◽  
Streptomyces Species ◽  
Isolation And Characterization ◽  
Test Organisms ◽  
Actinomycete Strain ◽  
Diffusion Assay ◽  
Strong Antimicrobial Activity

A new actinomycete strain was isolated from Western part of Bangladesh and identified as a new Streptomyces species on the basis of its morphological, biochemical, cultural characteristics and 16S rRNA data. The present paper describes the isolation and characterization of compound 1 from this new Streptomyces species with the help of various chemical and spectroscopic methods. Antimicrobial activity of compound 1 was tested by disc diffusion assay method and compared with that of standard antibiotics (Kanamycin for antibacterial activity and Nystatin for antifungal activity). The compound has been found to exhibit moderate to strong antimicrobial activity against the test organisms. Cytotoxicity of the compound 1 and the pet. ether extract of Czapek Dox (alkaline) broth of Streptomyces species was evaluated in brine shrimp bioassay with LC50 values of 23.85 µg/ml (ppm) and 19.95 µg/ml (ppm), respectively. Keywords: Streptomyces; Antimicrobial activity; Cytotoxicity. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.3079                  J. Sci. Res. 2 (1), 178-185 (2010)  

scholarly journals Potential of Bacteriocins from Lactobacillus taiwanensis for Producing Bacterial Ghosts as a Next Generation Vaccine

Toxins ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 432 ◽  
Author(s):  
Sam Woong Kim ◽  
Yeon Jo Ha ◽  
Kyu Ho Bang ◽  
Seungki Lee ◽  
Joo-Hong Yeo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Proteinase K ◽  
Next Generation ◽  
Cell Free Supernatant ◽  
Generation Vaccine ◽  
A Cell ◽  
Bacterial Ghosts ◽  
Functionally Diverse

Bacteriocins are functionally diverse toxins produced by most microbes and are potent antimicrobial peptides (AMPs) for bacterial ghosts as next generation vaccines. Here, we first report that the AMPs secreted from Lactobacillus taiwanensis effectively form ghosts of pathogenic bacteria and are identified as diverse bacteriocins, including novel ones. In detail, a cell-free supernatant from L. taiwanensis exhibited antimicrobial activities against pathogenic bacteria and was observed to effectively cause cellular lysis through pore formation in the bacterial membrane using scanning electron microscopy (SEM). The treatment of the cell-free supernatant with proteinase K or EDTA proved that the antimicrobial activity is mediated by AMPs, and the purification of AMPs using Sep-Pak columns indicated that the cell-free supernatant includes various amphipathic peptides responsible for the antimicrobial activity. Furthermore, the whole-genome sequencing of L. taiwanensis revealed that the strain has diverse bacteriocins, confirmed experimentally to function as AMPs, and among them are three novel bacteriocins, designated as Tan 1, Tan 2, and Tan 3. We also confirmed, using SEM, that Tan 2 effectively produces bacterial ghosts. Therefore, our data suggest that the bacteriocins from L. taiwanensis are potentially useful as a critical component for the preparation of bacterial ghosts.

scholarly journals Sponge-Associated Actinobacteria: Morphological Character and Antibacterial Activity against Pathogenic Bacteria

2017 ◽  
Vol 3 (1) ◽  
pp. 21-26
Author(s):  
SASMIATI FARACH DITA ◽  
SRI BUDIARTI ◽  
YULIN LESTARI
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Marine Ecosystem ◽  
Morphological Characteristics ◽  
Antibacterial Activities ◽  
Morphological Characters ◽  
Antimicrobial Activities ◽  
Shigella Dysenteriae ◽  
Rrna Gene

Sponge-associated actinobacteria may diverse and have potency to produce bioactive compounds. Diversity and antimicrobial activity of indigenous sponge-associated actinobacteria isolated from the marine ecosystem in Indonesia have not much been explored. This work aimed to assess morphological and antibacterial activity of sponge-associated actinobacteria. The morphological characteristics were examined based on their color of aerial and substrate mycelia, and pigmentation, while antibacterial activities were assayed using the antagonist technique. The selected actinobacterial isolate was identified using 16S rRNA gene. Various sponge-associated actinobacteria were successfully isolated from Hyrtios sp., Callyspongia sp., and Neofibularia sp. sponges. A total of 62 actinobacterial isolates were obtained, and each isolate showed a variety of morphological characters, which could be seen in aerial mass color, substrate mass color, and pigmentation. Actinobacterial isolates were tested against human pathogenic bacteria, i.e. Staphylococcus aureus and Methicillin-Resistant S. aureus, representing Gram-positive, and Escherichia coli EPEC K1-1 and Shigella dysenteriae, representing Gram-negative. Most of actinobacterial isolates had antimicrobial activities at least against one of pathogenic bacteria. High activity was shown by NOHa.2, isolated from Neofibularia, and HRHa.5 isolated from Hyrtios. The NOHa.2 showed the highest antimicrobial activity against S. dysenteriae, meanwhile, HRHa.5 showed antimicrobial activity against 3 of 4 tested bacterial pathogens. These data showed diversity of sponge-asccociated actinobacteria from marine ecosystem in Indonesia, and several of them have potency as source of antibacterial compounds

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity. Keywords:Antimicrobial,Sponges associated bacteria,MICs

scholarly journals Antimicrobial Activity of Essential Oil from Seeds of Carum carvi and Its Composition

1970 ◽  
Vol 25 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Jaripa Begum ◽  
M Nazrul Islam Bhuiyan ◽  
Jasmin Uddin Chowdhury ◽  
M Nuzmul Hoque ◽  
M Nural Anwar
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Phytopathogenic Fungi ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimum Fungicidal Concentration ◽  
Carum Carvi ◽  
Main Components

The essential oil of Carum carvi L. seeds was screened for its antimicrobial activity against ten pathogenic bacteria and six phytopathogenic fungi. The essential oil showed promising inhibitory activity against all the test bacteria, even at 2 ìl/disc. The minimum inhibitory concentration (MIC, 100-300 ppm) and minimum bactericidal concentration (MBC, 200-400 ppm) values of essential oil were determined. The antifungal screening of the essential oil showed 100% inhibition of radial mycelial growth of all the test fungi at 100 ppm. The MIC and minimum fungicidal concentration (MFC) values were found to vary from 50-300 ppm and 200-400 ppm respectively. The essential oil extracted by hydrodistillation from seeds of C. carvi was analyzed by gas chromatography-mass spectrometry (GC-MS). About 10 compounds had been identified in the seeds oils, accounting for more than 99.7% of the oils. The main components of the seeds oil were thymol (48.20%), o-cymene (19.29%), ϒ-terpinen (17.61%) and trimethylene dichloride (8.81%). Keywords: Antimicrobial activities; Carum carvi; Essential oil; GC-MS; ThymolDOI: http://dx.doi.org/10.3329/bjm.v25i2.4867 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 85-89

scholarly journals IN VITRO SCREENING OF ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OBTAINED FROM FICUS LYRATA WARB. (MORACEAE) LEAVES

2016 ◽  
Author(s):  
H. Tkachenko ◽  
L. Buyun ◽  
Z. Osadovskyy ◽  
M. Truhan ◽  
Ye. Sosnowski ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Traditional Use ◽  
Agar Disc ◽  
Ficus Lyrata

In the current investigation, screening of ethanolic extract obtained from Ficus lyrata leaves against pathogenic bacteria has been done in order to assess the antimicrobial activity aimed at detecting new sources of antimicrobial agents. The antimicrobial activity of the extract was determined using agar disc diffusion method. The antibacterial activity of leaf extract of F. lyrata was tested against human pathogenic bacteria — both Gram-positive (Staphylococcus aureus, methicillin-resistant S. aureus and Streptococcus pneumoniae) and Gram-negative strains (Klebsiella pneumoniae, Pseudomonas aeruginosa, and Escherichia coli). The results of this study provide evidence that the ethanolic extract of F. lyrata leaves has a mild antimicrobial activities, apparently, attributed to the presence of various secondary metabolites, which confirm the traditional use of this plant for the treatment of diseases caused by pathogens. These data allow us to suggest that the extracts of F. lyrata can be used to discover antibacterial substances for developing new pharmaceuticals to control clinically important pathogens responsible for severe disorders.

scholarly journals Agelasines B, D and antimicrobial extract of a marine sponge Agelas sp. from Tahuna Bay, Sangihe Islands, Indonesia

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
Walter Balansa ◽  
Stevy Imelda Murniati Wodi ◽  
Frets Jonas Rieuwpassa ◽  
Frans Gruber Ijong
Keyword(s):  
Antimicrobial Activity ◽  
Marine Sponge ◽  
Pathogenic Bacteria ◽  
Antimicrobial Drug ◽  
Flash Chromatography ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
New Antibiotics ◽  
Life Threatening ◽  
Strong Antimicrobial Activity

Abstract. Balansa W, Wodi SIM, Rieuwpassa FJ, Ijong FG. 2020. Agelasines B, D and antimicrobial extract of Agelas sp. from Tahuna Bay, Sangihe Islands, Indonesia. Biodiversitas 21: 699-706. The alarming growth of antibiotic-resistant bacteria necessitates the discovery of new antibiotics including those for combating life-threatening ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter sp) and fish pathogenic bacteria. This study aimed to identify antimicrobial compounds from an extract of a marine sponge collected from Enepahembang coral reef, Sangihe Islands, North Sulawesi, Indonesia. The sponge was identified by DNA barcoding as Agelas sp. and its extract was evaluated against three ESKAPE bacteria (S. aureus, K. pneumoniae, and A. baumannii) and three fish pathogenic bacteria (A. hydrophila, Edwardsiella tarda and Vibrio parahaemolyticus), using the standard disk diffusion method. It showed moderate to strong antimicrobial activity against S. aureus (25.3 mm), K. pneumoniae (15.5 mm), A. baumannii (20.2 mm), A. hydrophila (20.5 mm), E. tarda (22.4 mm) and A. salmonicida (21.2 mm). The extract was isolated by chromatographic techniques (column chromatography, flash chromatography, and high-performance liquid chromatography). The structures and relative stereochemistry of the two compounds were elucidated by HRESIMS, 1D and 2D NMR data analysis as well as by comparison with reported values. Unfortunately, limited amount of the pure compounds prevented us from further evaluating their antimicrobial activity against the test bacteria. Nevertheless, the crude extract's strong antimicrobial activity, especially against the test Gram-negative bacteria, suggests the importance of this finding in light of the recent antimicrobial drug scarcity but rapid antimicrobial resistance and the emerging paradigm of antimicrobial drug modification, redirection and/or repurposing for discovering new antibiotics particularly against the life-threatening Gram-negative bacteria.

Optimization of medium components to improve the antimicrobial activity of a new Saharan actinobacterial strain Saccharothrix tamanrassetensis DSM 45947

2021 ◽  
Vol 16 (7) ◽  
pp. 35-47
Author(s):  
Keramane Leila Bakour ◽  
Hadjira Boudjella ◽  
Nouredinne Bouras ◽  
Florence Mathieu
Keyword(s):  
Antimicrobial Activity ◽  
Antibiotic Production ◽  
Corn Steep Liquor ◽  
Basal Medium ◽  
Fermentation Medium ◽  
Antimicrobial Activities ◽  
Potential Candidate ◽  
Medium Components ◽  
Wide Range ◽  
Steep Liquor

Saccharothrix tamanrassetensis DSM 45947 previously isolated from a Saharan soil sample and characterized as a new species, was found to be a potential candidate in the search for novel antibiotics. The strain was found to exhibit a strong antimicrobial activity against a wide range of bacteria and fungi. In this study, statistical methods were applied to optimize the fermentation medium for enhanced antimicrobial activity production. A basal medium supplemented with sucrose and corn steep liquor was used as the original medium for optimization experiments. Plackett-Burman design showed that sucrose, corn steep liquor and KH2PO4, were recognised to have significant effect on antimicrobial activity production. Response surface methodology with Box-Behnken design determined the optimal concentrations. Optimal values for maximum antibiotic production were as follows: 7 g L-1 of sucrose, 52 g L-1 of corn steep liquor and 0.9 g L-1 of KH2PO4. Under these conditions, antimicrobial activities of DSM 45947 showed a global increase by 32% against the bacterium methicillinresistant Staphylococcus aureus and 36% against the fungus Umbelopsis ramanniana. This study provided an economical and efficient method to cultivate Saccharothrix tamanrassetensis DSM 45947 for enhanced antimicrobial activity production.

Extraction and Antimicrobial Activity of Total Flavones from Zanthoxylum nitidum

2011 ◽  
Vol 138-139 ◽  
pp. 1190-1194
Author(s):  
Xin She Li ◽  
Juan Hou
Keyword(s):  
Antimicrobial Activity ◽  
Rhodotorula Glutinis ◽  
Antimicrobial Activities ◽  
Chemotherapeutic Agents ◽  
Penicillium Citrinum ◽  
Microbial Infections ◽  
Zanthoxylum Nitidum ◽  
Strong Antimicrobial Activity ◽  
New Chemotherapeutic Agents

The total flavones in the root ofZanthoxylum nitidum(Roxb.) DC was extracted with 70% ethanol and the content of the total flavones in it was determined to be 82.9 mg/g by spectrophotometry at wavelength 510 nm based on rutin as standard. Thein vitroantimicrobial activities of the total flavones extract againstStaphylococcus aureus,Bacillus subtilis, Escherichia coli, Salmonella sp,Aspergillus niger,Aspergillus flavus, Penicillium citrinum, Saccharomyces cerevisiae, Rhodotorula glutinis, Aspergillus oryzaewere studied and the minimum inhibitory concentrations were measured to be 0.5, 1.0, 1.0, 1.0, 4.0, 1.0, 2.0, 2.0, 1.0, 1.0 mg/mL, respectively. The results indicate that the flavones inZanthoxylum nitidum (Roxb.)DC possesses strong antimicrobial activity and potentially will be useful for the development of new chemotherapeutic agents against microbial infections.

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