scholarly journals Effects of CaCl2 Treatment Alleviates Chilling Injury of Loquat Fruit (Eribotrya japonica) by Modulating ROS Homeostasis

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1662
Author(s):  
Yuanyuan Hou ◽  
Ziying Li ◽  
Yonghua Zheng ◽  
Peng Jin
Keyword(s):  
Superoxide Radical ◽  
Chilling Injury ◽  
Monodehydroascorbate Reductase ◽  
Gene Expressions ◽  
C Storage ◽  
Cycle System ◽  
Ros Homeostasis ◽  
Ros Metabolism ◽  
Antioxidant Enzymes Activities ◽  
Cacl2 Treatment

The effects of calcium chloride (CaCl2) treatment on chilling injury (CI), reactive oxygen species (ROS) metabolism, and ascorbate-glutathione (AsA-GSH) cycle in loquat fruit at 1 °C storage for 35 d were investigated. The results indicated that CaCl2 treatment remarkably suppressed the increase in browning index and firmness as well as the decrease in extractable juice rate. CaCl2 treatment also decreased the production of superoxide radical (O2•−), hydrogen peroxide (H2O2) content, but increased the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (OH•) scavenging ability, the activities of superoxide dismutase (SOD), catalase (CAT), and their gene expressions. Moreover, compared to the control loquat fruit, CaCl2-treated fruit maintained higher contents of AsA, GSH, higher levels of activities of ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR) and expressions of EjAPX, EjGR, EjMDHAR, and EjDHAR, but exhibited lower glutathione disulfide (GSSG) content. These results suggested that CaCl2 treatment alleviated CI in loquat fruit through enhancing antioxidant enzymes activities and AsA-GSH cycle system to quench ROS.

Navel Orange Pitting Stimulated by Waxing and High Storage Temperature

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 520b-520
Author(s):  
Huating Dou ◽  
Peter D. Petracek ◽  
Craig Davis
Keyword(s):  
High Temperature ◽  
Storage Temperature ◽  
Chilling Injury ◽  
Citrus Fruit ◽  
Navel Orange ◽  
Oil Glands ◽  
C Storage ◽  
High Storage Temperature ◽  
Florida Citrus ◽  
Citrus Varieties

Navel oranges are reportedly susceptible to postharvest peel disorders, including chilling injury and aging/stem end rind breakdown. These and other physiological disorders are sometimes given the common term “navel rind breakdown.” California citrus industry reports on recent incidences of navel rind breakdown suggested that some instances of this disorder were similar to “postharvest pitting,” a disorder that we have observed in a number of Florida citrus varieties. Thus, we decided to define the morphology and etiology of pitting of `Washington' navel orange (Citrus sinensis L.) peel. The disorder was characterized by the collapse of clusters of oil glands and was stimulated by wax application and high temperature (≥13 °C) storage. Internal ethanol levels of waxed fruit stored at high temperature (13 or 21 °C) were significantly higher among fruit that developed pitting than those that did not. The pitting observed in these studies is comparable to previously observed navel orange disorders that have occurred without known cause. Navel orange pitting is morphologically and etiologically distinct from chilling injury and aging/stem end rind breakdown, but is similar to postharvest pitting of Florida citrus fruit.

scholarly journals COLD TOLERANCE OF BANANA FRUITS OF DIFFERENT CULTIVARS

2016 ◽  
Vol 29 (3) ◽  
pp. 629-641 ◽  
Author(s):  
JOÃO ALISON ALVES OLIVEIRA ◽  
LUIZ CARLOS CHAMHUM SALOMÃO ◽  
DALMO LOPES DE SIQUEIRA ◽  
PAULO ROBERTO CECON
Keyword(s):  
Relative Humidity ◽  
Low Temperature ◽  
Cold Storage ◽  
Room Temperature ◽  
Storage Time ◽  
Chilling Injury ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Average Temperature ◽  
C Storage

ABSTRACT The objective of this work was to evaluate the tolerance of fruits of different banana cultivars to low temperature storages. Fruits of the cultivars Nanicão (AAA), Prata (AAB), Vitória (AAAB), Maçã (AAB) and Caipira (AAA) were used. Clusters of three fruits were kept in cold storage for 7, 14 and 21 days, with average temperature of 10.53±0.37°C and relative humidity of 85%. Subsequently, the clusters were transferred to temperatures of 22±0.39°C and evaluated for 16 days. The fruits of all cultivars remained green after 21 days of storage at 10.53±0.37°C. Fruits of the cultivar Nanicão did not completely ripened after transferred to the 22°C storage, when stored for 7 days at low temperature. These fruits were firmer, with green peel and low soluble solids and titratable acidity. The fruits of all cultivars complete the ripening when transferred to room temperature after 21 days of cold storage. Chilling injuries increased with cold storage time in all cultivars. The cultivars Nanicão, Caipira and Maçã had more symptoms of chilling injury, while Prata and Vitória were more tolerant to the cold storage (10.53°C) for up to 21 days, showing normal ripening after transferred to the 22±0.39°C storage.

Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage

2013 ◽  
Vol 40 (5) ◽  
pp. 449 ◽  
Author(s):  
Gabriela L. Müller ◽  
Claudio O. Budde ◽  
Martin A. Lauxmann ◽  
Agustina Triassi ◽  
Carlos S. Andreo ◽  
...  
Keyword(s):  
Cell Wall ◽  
Expression Profile ◽  
Tomato Fruit ◽  
Chilling Injury ◽  
Transcript Profile ◽  
Transcriptional Expression ◽  
Ethylene Evolution ◽  
Cell Wall Metabolism ◽  
C Storage ◽  
Fruit Decay

To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum (20°C). In this work, Micro-Tom tomatoes stored at 20°C (normal ripening) were compared with those stored at 15°C or 4°C (chilling injury inducer) for 7 days. In contrast to 4°C, storage at 15°C delayed ripening with the benefit of not enhancing oxidative metabolism and of enabling ripening upon being transferred to 20°C. The transcriptional expression profile of enzymes related to cell wall metabolism was compared at the three temperatures. Although endo-β-1,4-glucanase (Cel1), which is associated with fruit decay, was largely increased after removal from 4°C storage, its expression was not modified in fruits stored at 15°C. Enhanced transcriptional expression of xyloglucan endotransgylcosylase/hydrolases (XTHs) XTH1, –2, –10 and –11, and of two β-xylosidases (Xyl1–2) was detected in fruits stored at 15°C with respect to those at 20°C. Following 2 days at 20°C, these transcripts remained higher in fruits stored at 15°C and XHT3 and –9 also increased. Ethylene evolution was similar in fruits kept at 15°C and 20°C; thus, the changes in the transcript profile and fruit properties between these treatments may be under the control of factors other than ethylene.

scholarly journals Physiological and Molecular Mechanisms of ABA and CaCl2 Regulating Chilling Tolerance of Cucumber Seedlings

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2746
Author(s):  
Qian Feng ◽  
Sen Yang ◽  
Yijia Wang ◽  
Lu Lu ◽  
Mintao Sun ◽  
...  
Keyword(s):  
Low Temperature ◽  
Combined Treatment ◽  
Chilling Injury ◽  
Control Treatment ◽  
Water Control ◽  
Cucumber Seedlings ◽  
Injury Index ◽  
Mda Content ◽  
Cacl2 Treatment ◽  
Relative Electrical Conductivity

Cold stress is a limiting factor to the growth and development of cucumber in the temperate regions; hence, improving the crop’s tolerance to low temperature is highly pertinent. The regulation of low-temperature tolerance with exogenous ABA and CaCl2 was investigated in the cucumber variety Zhongnong 26. Under low-temperature conditions (day/night 12/12 h at 5 °C), seedlings were sprayed with a single application of ABA, CaCl2, or a combination of both. Our analysis included a calculated chilling injury index, malondialdehyde (MDA) content, relative electrical conductivity, antioxidant enzyme activities (SOD, CAT, and APX), leaf tissue structure, and expression of cold-related genes by transcriptome sequencing. Compared with the water control treatment, the combined ABA + CaCl2 treatment significantly improved the superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) of the seedlings by 34.47%, 59.66%, and 118.80%, respectively (p < 0.05), and significantly reduced the chilling injury index, relative electrical conductivity, and MDA content, by 89.47%, 62.17%, and 44.55%, respectively (p < 0.05). Transcriptome analysis showed that compared with the water control treatment, 3442 genes were differentially expressed for the combined treatment, 3921 for the ABA treatment, and 1333 for the CaCl2 treatment. KEGG enrichment analysis for both the ABA and combined ABA + CaCl2 treatments (as compared to the water control) showed that it mainly involves genes of the photosynthesis pathway and metabolic pathways. Differentially expressed genes following the CaCl2 treatment were mainly involved in plant hormone signal transduction, plant–pathogen interaction, MAPK signaling pathway–plant, phenylpropanoid biosynthesis, and circadian rhythm–plant. qRT-PCR analysis and RNA-seq results showed a consistent trend in variation of differential gene expression. Overall, this study demonstrated that although all three treatments provided some protection, the combined treatment of ABA (35 mg/L) with CaCl2 (500 mg/L) afforded the best results. A combined ABA + CaCl2 treatment can effectively alleviate cold-stress damage to cucumber seedlings by inducing physiological changes in photosynthesis and metabolism, and provides a theoretical basis and technical support for the application of exogenous ABA and CaCl2 for low-temperature protection of cucumber seedlings.

scholarly journals Prestorage Application of Oxalic Acid to Alleviate Chilling Injury in Mango Fruit

HortScience ◽  
2015 ◽  
Vol 50 (12) ◽  
pp. 1795-1800 ◽  
Author(s):  
Peiyan Li ◽  
Xiaolin Zheng ◽  
Md. Golam Ferdous Chowdhury ◽  
Kim Cordasco ◽  
Jeffrey K. Brecht
Keyword(s):  
Oxalic Acid ◽  
Adenosine Triphosphatase ◽  
Superoxide Radical ◽  
Mangifera Indica ◽  
Chilling Injury ◽  
Membrane Integrity ◽  
Mango Fruit ◽  
Typically Develop ◽  
Energy Cycle ◽  
Fruit Decay

Effects of postharvest oxalic acid (OA) application on chilling injury (CI) in harvested mango fruit (Mangifera indica L.) were investigated using ‘Tommy Atkins’ fruit from Florida and ‘Zill’ fruit from Panzhihua. The OA was applied to harvested fruit as a 5 or 10 mm drench for 10 or 15 minutes at 25 °C. ‘Tommy Atkins’ fruit typically develop external CI symptoms while ‘Zill’ develops internal symptoms. Development of CI symptoms was significantly reduced in OA-treated ‘Tommy Atkins’ fruit stored for 18 days at 5 °C as was the rate of softening upon transfer to 25 °C for 4 days. However, OA treatment did not substantially control fruit decay. For ‘Zill’, CI development was significantly reduced in OA-treated fruit during storage at 10 °C for 49 days and subsequently for 4 days at 25 °C. In addition, membrane integrity was enhanced and the activities of the antioxidant system enzymes superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were elevated, although there were decreases in both hydrogen peroxide (H2O2) content and superoxide radical production in OA-treated fruit. The activities of some enzymes of the energy cycle were also elevated in the OA-treated fruit, including succinate dehydrogenase (SDH), cytochrome C oxidase (CCO), H+-adenosine triphosphatase (H+-ATPase), and Ca2+-adenosine triphosphatase (Ca2+-ATPase). Thus, OA may enhance CI tolerance in mango fruit by maintaining membrane integrity associated with enhanced antioxidant activity and regulation of energy metabolism. Application of 5 mm OA appears to be beneficial in controlling postharvest CI in mango fruit.

scholarly journals Transcriptional Regulation of ROS Homeostasis by the ERR Subfamily of Nuclear Receptors

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 437
Author(s):  
Charlotte Scholtes ◽  
Vincent Giguère
Keyword(s):  
Transcription Factors ◽  
Transcriptional Control ◽  
Large Body ◽  
Bacterial Invasion ◽  
Ros Generation ◽  
Cellular Energy ◽  
Metabolic Genes ◽  
Ros Homeostasis ◽  
Ros Metabolism ◽  
Antioxidant Mechanisms

Reactive oxygen species (ROS) such as superoxide anion (O2•−) and hydrogen peroxide (H2O2) are generated endogenously by processes such as mitochondrial oxidative phosphorylation, or they may arise from exogenous sources like bacterial invasion. ROS can be beneficial (oxidative eustress) as signaling molecules but also harmful (oxidative distress) to cells when ROS levels become unregulated in response to physiological, pathological or pharmacological insults. Indeed, abnormal ROS levels have been shown to contribute to the etiology of a wide variety of diseases. Transcriptional control of metabolic genes is a crucial mechanism to coordinate ROS homeostasis. Therefore, a better understanding of how ROS metabolism is regulated by specific transcription factors can contribute to uncovering new therapeutic strategies. A large body of work has positioned the estrogen-related receptors (ERRs), transcription factors belonging to the nuclear receptor superfamily, as not only master regulators of cellular energy metabolism but, most recently, of ROS metabolism. Herein, we will review the role played by the ERRs as transcriptional regulators of ROS generation and antioxidant mechanisms and also as ROS sensors. We will assess how the control of ROS homeostasis by the ERRs can be linked to physiology and disease and the possible contribution of manipulating ERR activity in redox medicine.

scholarly journals Effect of Postharvest Temperature and Storage Duration on Growth and Flowering of the Phalaenopsis Orchid

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 517E-517
Author(s):  
Yin-Tung Wang
Keyword(s):  
Air Temperature ◽  
Chilling Injury ◽  
Late Summer ◽  
Temperature Treatment ◽  
Storage Duration ◽  
Subsequent Performance ◽  
C Storage ◽  
Bare Root ◽  
And Storage ◽  
Storage Temperatures

On 6 Sept. 1996, container-grown vegetatively propagated Phalaenopsis Atien Kaala `TSC22' plants were harvested and individually weighed. The bare-root plants were packed in cartons with shredded newspaper and placed in incubators at 15, 20, 25, or 30°C air temperature. Control plants were undisturbed. After 4, 7, or 14 days, one-third of the plants were removed from each temperature treatment, weighed, planted in pots, and then placed in a greenhouse. Mass loss (primarily water) increased with increasing air temperature and duration in storage. Symptoms of chilling injury (yellow blotches on leaves) were inversely related to 15 and 20°C storage temperatures. Chilling injury became more severe as storage duration increased. Plants had little or no chilling injury at 25 and 30°C, regardless of storage duration. Leaf loss was most severe on plants stored at 15°C for 7 or 14 days or at 30°C for 14 days. Increased storage duration up to 14 days did not affect the time of spiking (appearance of the flowering shoot) for plants stored between 15 and 25°C. Those kept at 30°C, regardless of the duration, spiked 5 to 8 days after the control. The results suggest that vegetative Phalaenopsis plants harvested in late summer should be stored and shipped at 25°C. Under such conditions, plants could lose 20% of the fresh mass between harvesting and planting without adversely affecting subsequent performance.

scholarly journals Light-Dependent Oxidative Stress Determines Physiological Leaf Spot Formation in Barley

2004 ◽  
Vol 94 (6) ◽  
pp. 584-592 ◽  
Author(s):  
Yue-Xuan Wu ◽  
Andreas von Tiedemann
Keyword(s):  
Oxidative Stress ◽  
Leaf Spot ◽  
Spring Barley ◽  
Light Stress ◽  
Field Conditions ◽  
Stress Factors ◽  
Monodehydroascorbate Reductase ◽  
Sod Activity ◽  
Active Radiation ◽  
Ros Metabolism

We reported previously that physiological leaf spot (PLS) formation in winter and spring barley is dependent on genotype-related oxidative stress under field conditions. In the present study, we searched for factors inducing PLS symptoms in the greenhouse similar to those observed in the field and investigated its relationship to reactive oxygen species (ROS) metabolism. We found that in the greenhouse, oxidative stress induced spring barley cv. Extract, which is sensitive to PLS, to express symptoms similar to those observed in the field. Leaves severely affected by PLS showed significantly lower activities of key enzymes in the Halliwell-Asada cycle such as ascorbate peroxidase, glutathione reductase, dehy-droascorbate reductase, and monodehydroascorbate reductase. The sensitive cultivar also showed lower levels of total superoxide dismutase (SOD) and Cu/Zn-SOD activity but a higher level of chloroplast-specific Fe-SOD activity than that of the insensitive cultivar. Thus, an unbalanced ROS metabolism in chloroplasts may trigger PLS incidence in sensitive cultivars, which is in agreement with the fact that light is essential for the induction of PLS expression under both field and greenhouse conditions. Accordingly, under greenhouse conditions, continuous light stress (7 days), but not light shock treatments, induced PLS similar to that of field conditions in sensitive cv. Extract, but not in resistant cv. Scarlett. Light with a high proportion of energy in the blue wavelength spectrum (350 to 560 nm) was significantly more PLS inductive than light with a pronounced red (photosynthetically active radiation) spectrum (580 to 650 nm). Exposure to ozone did not produce PLS-like symptoms. Furthermore, similar to earlier observations in the field, PLS symptom expression was closely correlated with the accumulation of superoxide (O2 •-) detected by both biochemical and histochemical assays. Taken together, these data suggest that PLS in barley is genotype-dependent but its expression appears to be induced by certain environmental stress factors, among which photosyn-thetically active radiation plays a major role.

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