scholarly journals Milk Production and Quality of Lactating Yak Fed Oat Silage Prepared with a Low-Temperature-Tolerant Lactic Acid Bacteria Inoculant

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2437
Author(s):  
Mingming Zhu ◽  
Rongqing Xie ◽  
Liangyin Chen ◽  
Minghong You ◽  
Wenlong Gou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lactic Acid ◽  
Low Temperature ◽  
Lactic Acid Bacteria ◽  
Milk Yield ◽  
Dry Matter ◽  
Dry Matter Intake ◽  
Fresh Matter ◽  
Total N

This study aimed to investigate the effect of oat silage treated with a low-temperature-tolerant lactic acid bacteria (LAB) inoculant on milk yield and the quality of lactating yaks. Oat silages were prepared in big round bales, treated without (control) or with a low-temperature-tolerant LAB inoculant (a mixture of Lactobacillus plantarum BP18, Pediococcus pentosaceus HS1 and Lactobacillus buchneri LP22; the application rate of 105 cfu/g on a fresh matter basis). Eighteen lactating yaks were divided into nine pairs with a similar milk yield. Each pair of yaks was randomly allocated to the control or LAB-inoculated silage treatment. The inoculated silage increased the dry matter intake and the total volatile fatty acid (mainly acetate, propionate and butyrate) in rumen fluid compared with the control. The inoculated silage also enhanced the yield of yak milk with high contents of total N, fat and lactose. In addition, high levels of essential amino acids (Thr, Leu and Phe), polyunsaturated fatty acids and low saturated fatty acids were observed in milk when lactating yaks were fed with the inoculated silage. Therefore, inoculation with a low-temperature-tolerant LAB during ensiling could promote the milk yield of lactating yaks by enhancing dry matter intake and ruminal fermentation.

Effects of plants containing secondary metabolites as feed additives on rumen metabolites and methanogen diversity of buffaloes

2016 ◽  
Vol 56 (3) ◽  
pp. 472 ◽  
Author(s):  
L. Samal ◽  
L. C. Chaudhary ◽  
N. Agarwal ◽  
D. N. Kamra
Keyword(s):  
Fatty Acids ◽  
Lactic Acid ◽  
Secondary Metabolites ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Feed Additives ◽  
Dry Matter Intake ◽  
Control Group ◽  
Rumen Ph ◽  
Pooled Samples

Four fistulated adult Murrah buffaloes were fed on a basal diet consisting of wheat straw and concentrate mixture in a 4 × 4 Latin square design to study the effects of feeding plants containing secondary metabolites on rumen metabolites and methanogen diversity. The four groups were Control (no additive), Mix-1 (ajwain oil and lemon grass oil in a 1 : 1 ratio @ 0.05% of dry matter intake), Mix-2 (garlic and soapnut in a 2 : 1 ratio @ 2% of dry matter intake) and Mix-3 (garlic, soapnut, harad and ajwain in a 2 : 1 : 1 : 1 ratio @ 1% of dry matter intake). In each phase of 30 days’ duration, after 19 days of feeding, rumen liquor was sampled for two consecutive days at 0, 2, 4, 6 and 8 h post-feeding, whereas rumen content was sampled at 0 h feeding. The pH of the rumen liquor was recorded at every collection and then the rumen liquor of every collection was pooled day-wise and animal-wise. These pooled samples were used for estimation of rumen metabolites like ammonia, lactic acid and volatile fatty acids. Microscopic counting of protozoa was done in both 0 h and pooled samples of rumen liquor. Rumen contents collected from different locations of rumen were processed for enzyme estimation. The rumen contents were squeezed and the liquid portion was used for DNA isolation, which was further processed to determine methanogen diversity. Daily intake of feed was similar (P > 0.05) in all the four groups. The ammonia-N concentration and ciliate protozoa population were reduced significantly in the treatment groups supplemented with additives. Rumen pH, lactic acid, volatile fatty acids and enzyme activities were not affected (P > 0.05) by feeding of any of these additives. Methanogenic diversity comparison was made between the Control and Mix-1 group. The basic local alignment search tool (BLAST) analysis of the 133 (44 from the Control group and 89 from the Mix-1 group) sequences showed similarity of the sequences of rumen archaea by up to 97% to the known sequences of rumen methanogens. The sequences with minimum length of 750 bp were selected for phylogenetic analysis. Per cent identity of these sequences with that of the available nearest neighbour as calculated by MEGA 5.03 software showed identity of the clones in the range of 88–97%. The clones were similar with Methanobrevibacter smithii ATCC 35061, uncultured Methanobrevibacter sp. clone MEME95 and M. ruminantium M1. Overall, feeding of any of these feed additives to fistulated buffaloes did not affect feed intake, rumen pH, or rumen metabolites except ammonia and enzyme profile. Methanogen diversity showed the possibility of Methanobrevibacter as the major methanogen in buffalo rumen liquor.

Improving the fermentation quality of wheat straw silage stored at low temperature by psychrotrophic lactic acid bacteria

2016 ◽  
Vol 88 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Miao Zhang ◽  
Haoxin Lv ◽  
Zhongfang Tan ◽  
Ya Li ◽  
Yanping Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Low Temperature ◽  
Lactic Acid Bacteria ◽  
Wheat Straw ◽  
Fermentation Quality

Impact inoculum dosage of lactic acid bacteria on oat and wheat silage fermentation at ambient and low temperatures

2018 ◽  
Vol 69 (12) ◽  
pp. 1225
Author(s):  
Miao Zhang ◽  
Zhongfang Tan ◽  
Xiaojie Wang ◽  
Meiyan Cui ◽  
Yanping Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Low Temperature ◽  
Lactic Acid Bacteria ◽  
Ambient Temperature ◽  
Low Temperatures ◽  
Raw Materials ◽  
Antimicrobial Properties ◽  
Alpine Regions ◽  
Silage Quality

Lactic acid bacteria (LAB) can be used as silage additives to ensure rapid and vigorous fermentation at early stages of ensiling. We predicted that the optimal LAB inoculation dosage for forage at ambient temperature (15–38°C) would be different from that at cold temperature (4°C). In this study, Lactobacillus plantarum QZ227, isolated from a wheat landrace in alpine regions of Qinghai, China, and commercial L. plantarum FG1 were used as inoculum, with sterile water as control. The effects of inoculum dosage on the fermentation quality of oat (Avena sativa L. cv. Qinghai) and wheat (Triticum aestivum L. cv. Yumai No. 1) silage at ambient temperature (15–38°C) and at 4°C were investigated in laboratory experiments. Little or no improvement in silage quality occurred upon increasing the inoculum dosage at ambient temperature. By contrast, a lower pH and NH3-N content, and a higher LAB count and lactic acid content, were observed at 4°C. Furthermore, the growth of Escherichia coli was inhibited effectively at the lower temperature, and silage quality was positively correlated with increasing inoculum dosage (P<0.05). These results suggest that increasing the inoculum dosage could improve the quality of silage at lower temperatures of 4°C, whereas an appropriate dosage was a key factor for silage at ambient temperature. In this study, oat forage could be used as raw materials for ensiling only at low temperature but was not suitable for ensiling at ambient temperature without wilting due to the high moisture content (86.55%). QZ227 isolated from the roots of wheat in alpine regions displayed superior antimicrobial properties against yeast at ambient temperature and E. coli at 4°C compared with commercial strain FG1. This is the first study to explore the effects of inoculum dosage on silage quality at low temperatures, and provides a basis for low-temperature silage technologies.

scholarly journals Prepartum and Postpartum Feed Restrictions Affect Blood Metabolites and Hormones Reducing Colostrum and Milk Yields in Fat-Tailed Dairy Sheep

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1258
Author(s):  
Mousa Zarrin ◽  
Meysam Sanginabadi ◽  
Mahrokh Nouri ◽  
Amir Ahmadpour ◽  
Lorenzo E. Hernández-Castellano
Keyword(s):  
Fatty Acids ◽  
Milk Yield ◽  
Dry Matter ◽  
Relative Increase ◽  
Dry Matter Intake ◽  
Blood Metabolites ◽  
Feed Restriction ◽  
Dairy Sheep ◽  
Ctrl Group ◽  
Beta Hydroxybutyrate

This study aimed to investigate the effect of prepartum and postpartum feed restriction on body weight (BW), blood metabolites, and hormones as well as colostrum and milk yields and compositions in fat-tailed dairy sheep. In this study, 20 multiparous and pregnant ewes were randomly allocated to either the control (Ctrl; n = 10) or the feed-restricted (FR; n = 10) groups from week −5 to week 5 relative to parturition. Despite dry matter intake being decreased in the FR group compared to the Ctrl throughout both prepartum and postpartum periods, no differences in BW were detected between groups in any of the studied periods. Feed restriction increased both free fatty acids and beta-hydroxybutyrate concentrations during both prepartum and postpartum periods. Similarly, feed restriction increased triglyceride concentration postpartum. Additionally, feed restriction increased insulin and growth hormone and decreased prolactin concentrations during both prepartum and postpartum periods. Feed restriction caused a decreased colostrum yield and a relative increase of the main colostrum components in the FR group. Similarly, milk yield decreased in the FR group compared to the Ctrl group, although milk components were not affected. In conclusion, feed restriction did not affect BW but decreased colostrum and milk yield in fat-tailed dairy sheep.

scholarly journals Microbiological and Physicochemical Characteristics of Inasua Traditional Fish Fermented from Maluku Islands

2018 ◽  
Vol 10 (2) ◽  
pp. 298-305
Author(s):  
Ferymon Mahulette ◽  
Nisa Rachmania Mubarik ◽  
Antonius Suwanto ◽  
Widanarni Widanarni
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Water Activity ◽  
Raw Materials ◽  
Physicochemical Characteristics ◽  
Proximate Analysis ◽  
Number Of Bacteria

Based on the raw materials, inasua consists of two types namely inasua with sap and inasua without sap. Research of inasua with sap has never been done and considered as the novelty of this research. The sensory characteristics and shelf life of two types of inasua were different. The research aims to analyze the microbiological and physicochemical characteristics of two types of inasua during fermentation. The microbiological analyzes include the total number of bacteria and lactic acid bacteria, while physicochemical analyzes include temperature, pH, water activity, proximate analysis, salt, alcohol, histamine, amino acids and fatty acids contents. The total number of bacteria and lactic acid bacteria has decreased during fermentation. At the end of the fermentation the total number of bacteria and lactic acid bacteria inasua with sap were 3.2x107 CFU/g and 3.0x107 CFU/g, while inasua without sap were 5.4x105CFU/g and 3.5x105 CFU/g, respectively. The moisture, protein, alcohol contents and water activity decreased, otherwise the salt, fat, ash, amino acids, and fatty acids contents increased during fermentation. Generally, microbiological and physicochemical characteristics of inasua with sap was better than inasua without sap. The results of this research to improve the quality of this fermentation product in the future.

EFFECT OF ADDITIVES CONTAINING LACTIC ACID BACTERIA AND/OR HYDROLYTIC ENZYMES WITH AN ANTIOXIDANT UPON THE PRESERVATION OF CORN OR ALFALFA SILAGE

1981 ◽  
Vol 61 (3) ◽  
pp. 669-680 ◽  
Author(s):  
J. G. BUCHANAN-SMITH ◽  
Y. T. YAO
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Crude Protein ◽  
Dry Matter ◽  
Hydrolytic Enzymes ◽  
Water Soluble ◽  
Soluble Carbohydrate ◽  
Total N ◽  
Alfalfa Silage ◽  
Water Soluble Carbohydrate

A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity). The additive did not affect (P > 0.05) final pH, lactic or acetic acid concentration, crude protein or NPN-N and NH4+-N expressed as a percent of total N. Recovery of energy from treated silage was greater than control in the two pairs of silos where this was determined, but recoveries of dry matter and crude protein were not consistently affected. Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos. The experimental design used a4 (DM level) × 4 (added glucose — 0,4,8 and 12%, DM basis) × 3 (additive — control, a hydrolytic enzyme/antioxidant additive (E/AO), and E/AO plus lactic acid bacteria (E/AO +)) factorial arrangement of treatments. Alfalfa was harvested using farm equipment. For 60-day silage, the additives as either a primary factor in the design or in second-order interactions with either silage DM or glucose showed no effect (P > 0.05) upon pH, lactic acid, acetic acid, butyric acid, Fleig score, residual water-soluble carbohydrate and percent of total N as NPN-N or ammonia-N. Poor quality silage resulted from the fermentation of alfalfa at 20 and 30% DM, and although additional glucose resolved this problem the additives did not. Beneficial effects of additional water-soluble carbohydrate in alfalfa silage fermentation are evident in data presented. Thus an additive containing an antioxidant and hydrolytic enzymes was not very effective and numbers of lactic acid bacteria in untreated alfalfa harvested with farm equipment must already be sufficient for adequate fermentation.

scholarly journals Effects of different amounts of crude glycerol supplementation on dry matter intake, milk yield, and milk quality of lactating dairy cows grazing on a Kikuyu grass pasture

2021 ◽  
Vol 12 (4) ◽  
pp. 491-497
Author(s):  
Diana Marcela Valencia E ◽  
Luis Alfonso Giraldo ◽  
Alejandra Marin ◽  
Yury Tatiana Granja Salcedo ◽  
Telma Teresinha Berchielli
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Crude Glycerol ◽  
Milk Quality ◽  
Dry Matter Intake ◽  
Lactating Dairy Cows ◽  
Kikuyu Grass

Supplementing high-yielding cows at pasture with concentrates fed at a level determined by milk yield and season

1962 ◽  
Vol 59 (2) ◽  
pp. 233-244 ◽  
Author(s):  
R. Laird ◽  
J. Walker-Love
Keyword(s):  
Milk Yield ◽  
Dry Matter ◽  
Supplementary Feeding ◽  
Dry Matter Intake ◽  
Considerable Controversy ◽  
The Subject ◽  
Yield Time

The practice of supplementary feeding of concentrates has been the subject of much investigation and also the centre of considerable controversy during the last decade. The latter is perhaps not hard to explain as so many variables are present; first and foremost there is the cow which at all times must be considered an individual whether it be milk yield, time of calving or dry-matter intake. In addition, one must always take into account the quantity and quality of grass, both of which are dependent on sward management and the system of grazing.

scholarly journals EFFECT OF DIFFERENT ADDITIVES ON THE SILAGE QUALITY OF SWEET POTATO BRANCHES

2019 ◽  
Vol 32 (2) ◽  
pp. 506-513
Author(s):  
NERMY RIBEIRO VALADARES ◽  
VALTER CARVALHO DE ANDRADE JUNIOR ◽  
ROSANA CRISTINA PEREIRA ◽  
CÍNTIA MARIA TEIXEIRA FIALHO ◽  
MARCOS AURÉLIO MIRANDA FERREIRA
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Sweet Potato ◽  
Crude Protein ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Mineral Matter ◽  
Citrus Pulp ◽  
Silage Quality

ABSTRACT Sweet potato branches have potential use in animal feeding, even fresh or ensiled, and some additives may enhance their quality. This study aimed at assessing silage quality of sweet potato branches using different additives. We ensiled sweet potato branches without additive (control) and with the addition of 10% disintegrated corn with straw and corncob (DCSC), 10% sugarcane bagasse from alembic, 10% citrus pulp, and 10% coffee husk. Three samples of each plot were obtained after drying for measurements of dry matter, crude protein, neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose, hemicellulose, lignin, mineral matter, starch, soluble sugars, pH, lactic acid bacteria population, gas, and effluent losses. Silage with DCSC, citrus pulp, and coffee rusk reached the highest dry matter contents. Moreover, citrus pulp silage had a high content of NDF. In contrast, DCSC silage showed the lowest content of ADF. Lignin was low in silage with DCSC, citrus pulp, and control (without additive). Suitable amounts of starch, crude protein, and lactic acid bacteria population were found in all treatments. Silage with DCSC achieved the best results for the assessed variables. Therefore, DCSC is the most suitable additive to improve silage quality of sweet potato branches.

scholarly journals Physicochemical and Microbiological Characteristics from “Wagashi” Production Whey in Abomey-Calavi (Benin)

2021 ◽  
pp. 61-69
Author(s):  
Oumarou Djobo ◽  
Abdou Hamidou Soule ◽  
Haziz Sina ◽  
Souriatou B. Tagba ◽  
Farid Baba-Moussa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Dry Matter ◽  
Titratable Acidity ◽  
Fecal Coliforms ◽  
Microbiological Characteristics ◽  
Aerobic Flora ◽  
Yeasts And Molds

Aims: The production of “wagashi”, induce the production of whey which is often directly drop in the environment. The aim of this study was to evaluate the microbiological and physicochemical qualities of wagashi’s whey samples collected in Abomey-Calavi (Benin). Methodology: Whey samples were collected from Abomey-Calavi (Parana and Akassato) in Southern Benin. The physicochemical analyses targeted the pH, titratable acidity, dry matter contents, protein and lactose. The microbiological analyzes carried out consisted in enumeration of total mesophilic flora (TMF), fecal coliforms, lactic acid bacteria, staphylococci, yeasts and molds, Pseudomonas, Escherichia coli and salmonella by cultures on specific synthetic nutrient media. Results: This study revealed characteristics such as dry matter (5.30-5.66 g / l), pH (3.91-5.21), titratable acidity, protein (5.94-0.128 g / l), lactose; and microbial quality of the whey. Parana’s whey was more acidic (pH = 3.91 ± 0.014) than Akassato’s whey analyzed with higher titratable acidity (pH = 0.92 ± 0.01). The presence of total mesophilic aerobic flora, Escherichia coli, staphylococci, lactic acid bacteria, yeasts and molds reveals fecal, human and environmental contamination during cheese production or during whey storage. Thus, Parana's whey was more contaminated with TMF (3.45.108 CFU/ml), lactic acid bacteria (4.82.108 CFU/ml) and fecal coliforms (> 3.108 CFU/ml) while Akassato's whey was more contaminated by staphylococci (4.70.108 CFU/ml) and Escherichia coli (> 3.108 CFU/ml). Salmonella was not identified. Conclusion: It thus important raising the awareness of cow's milk processors and / or whey producers in Benin. We therefore project to use this whey as a substrate for bioproduction.

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