scholarly journals Bioactivity of Essential Oils for Mitigation of Listeria monocytogenes Isolated from Fresh Retail Chicken Meat

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3006
Author(s):  
Alaa Eldin M. A. Morshdy ◽  
Mohammed S. Al-Mogbel ◽  
Mohamed E. M. Mohamed ◽  
Mohamed Tharwat Elabbasy ◽  
Azza K. Elshafee ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Essential Oils ◽  
Foodborne Pathogens ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Ion Leakage ◽  
Capacity Loss ◽  
Retail Chicken Meat ◽  
Safety Applications

Listeria monocytogenes is one of the most severe foodborne pathogens found in several habitats. Therefore, this study aims to investigate the antilisterial activity of different essential oils (EOs) against multidrug-resistant (MDR) L. monocytogenes strains isolated from fresh chicken meat. Our results showed that the prevalence of L. monocytogenes in the examined samples was 48%. Seventy-eight isolates were identified as L. monocytogenes. Out of these, 64.1% were categorized as MDR and were categorized in 18 patterns with 50 MDR isolates. One isolate was selected randomly from each pattern to investigate their biofilm-forming ability, resistance, and virulence genes incidence. Out of 18 MDR isolates, 88.9% showed biofilm-forming ability. Moreover, the most prevalent resistance genes were ermB (72%), aadA (67%), penA (61%), and floR genes (61%). However, the most prevalent virulence genes were inlA (94.4%), prfA (88.9%), plcB (83.3%), and actaA (83.3%). The antilisterial activity of EOs showed that cinnamon bark oil (CBO) was the most effective antilisterial agent. CBO activity could be attributed to the bioactivity of cinnamaldehyde which effects cell viability by increasing the bacterial cell electrical conductivity, ion leakage, and salt tolerance capacity loss. Therefore, CBO could be an effective alternative natural agent for food safety applications.

scholarly journals Prevalence and antimicrobial resistance of Salmonella serotypes isolated from retail chicken meat and giblets in Iran

2015 ◽  
Vol 9 (05) ◽  
pp. 463-469 ◽  
Author(s):  
Hamid Reza Sodagari ◽  
Zohreh Mashak ◽  
Amir Ghadimianazar
Keyword(s):  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Chicken Meat ◽  
Resistance Testing ◽  
Retail Markets ◽  
Predominant Type ◽  
Amoxicillin Clavulanic Acid ◽  
Resistant Strains ◽  
Resistance Rates ◽  
Retail Chicken Meat

Introduction: Salmonella is one of the major foodborne pathogens responsible for outbreaks of foodborne illness in humans worldwide. Methodology: A total of 560 samples of chicken meat and giblets were collected from retail markets for Salmonella identification, serotyping, and antimicrobial resistance testing. Results: Salmonella was detected in 19.8% of samples. Among the five serotypes identified, S. Thompson was the predominant type (48.7%). High antimicrobial resistance rates were observed to nalidixic acid (92.8%), tetracycline (81%), trimethoprim (68.4%), sulfamethoxazole / trimethoprim (61.2%), streptomycin (56.7%), and kanamycin (36.9%). Although resistance to chloramphenicol (3.6%), amoxicillin-clavulanic acid (5.4%), and ampicillin (11.7%) was detected, none of the isolates were resistant to ceftazidime, ceftriaxone, cefotaxime, ciprofloxacin, colistin, gentamicin, nor imipenem. Conclusions: Restrictions on the irrational use of antibiotics in humans and animals are suggested for the reduction of resistant strains.

Antimicrobial activity of essential oils against foodborne multidrug-resistant enterococci and aeromonads in planktonic and biofilm state

2018 ◽  
Vol 25 (2) ◽  
pp. 101-108 ◽  
Author(s):  
Ana P Quendera ◽  
António S Barreto ◽  
Teresa Semedo-Lemsaddek
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Foodborne Pathogens ◽  
Antimicrobial Effect ◽  
Multidrug Resistant ◽  
Thymus Vulgaris ◽  
Cymbopogon Flexuosus ◽  
Subsequent Determination ◽  
Colony Counting ◽  
Enterococcus Spp

The selection and use of natural compounds with antimicrobial activity against foodborne pathogens is of major importance. The present study evaluated the antimicrobial activity of commercial essential oils against multidrug-resistant Enterococcus spp. and Aeromonas spp. Cymbopogon flexuosus and Thymus vulgaris essential oils presented the highest inhibitory zones against both bacterial groups ( p < 0.05). Subsequent determination of the minimum inhibitory concentrations showed values between 0.47 and 1.9 mg/ml for Aeromonas spp. and from 1.9 to 15 mg/ml for Enterococcus spp. The antimicrobial effect of C. flexuosus and T. vulgaris essential oils was also assessed against biofilms. Bacteria in biofilm state were subjected to 30 min or 1 h of exposure to each essential oil and eradication ability estimated by colony counting. Both essential oils exhibited antimicrobial activity against preformed Aeromonas biofilms, but were unable to successfully eradicate biofilms produced by enterococci, in the conditions under investigation. Nonetheless, the presumptive use of essential oils in the food industry should be considered in further investigations.

scholarly journals Phenotypes, antibacterial-resistant profile, and virulence-associated genes of Salmonella serovars isolated from retail chicken meat in Egypt

2020 ◽  
Vol 13 (3) ◽  
pp. 440-445
Author(s):  
Amal Awad ◽  
Mayada Gwida ◽  
Eman Khalifa ◽  
Asmaa Sadat
Keyword(s):  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Chicken Meat ◽  
Poultry Meat ◽  
Diffusion Method ◽  
Poultry Production ◽  
Production Chain ◽  
Salmonella Serovars ◽  
Meat Samples ◽  
Retail Chicken Meat

Aim: The present study was designed to investigate the occurrence and distribution of Salmonella serotypes in chicken meat samples, and to explore the susceptibility of the strains to antimicrobials, as well as their virulence-associated genes. Materials and Methods: Two-hundred retail chicken meat samples from different shops, as well as 25 stool specimens from retail shop workers, were included in the study. The collected samples were examined bacteriologically for the presence of salmonellae. Salmonella isolates were serotyped using a slide agglutination test for O and H antigens and were screened for the presence of five virulence genes (stn, pef, invA, sopB, and avrA) using a uniplex polymerase chain reaction assay and for their susceptibility to 18 antimicrobial agents using the disk diffusion method. Results: Thirty-one Salmonella isolates belonging to 12 different serovars were identified. Salmonella Enteritidis and Salmonella Kentucky were the dominant serovars (22.6% each). Salmonella isolates displayed a high antibiotic resistance against erythromycin, sulfamethoxazole/trimethoprim, doxycycline, cephalexin, cefaclor, tetracycline, polymyxin B, cefuroxime, vancomycin, and streptomycin. All Salmonella isolates exhibited multidrug resistance (MDR) and demonstrated different virulence genes. The majority of Salmonella serovars (87.1%) harbored sopB gene, 54.8% carried avrA and pef genes, while all isolates carried invA and stn genes. Conclusion: The presence of virulent MDR Salmonellae in raw chicken meat could allow the possibility of transmission of these resistant serovars to humans. Therefore, strict hygienic measures should be followed on the whole poultry production chain to decrease the potential transmission of Salmonella infection from poultry meat to humans.

Multidrug-Resistant Salmonella Isolates from Retail Chicken Meat Compared with Human Clinical Isolates

2010 ◽  
Vol 7 (8) ◽  
pp. 929-934 ◽  
Author(s):  
Nkuchia M. M'ikanatha ◽  
Carol H. Sandt ◽  
A. Russell Localio ◽  
Deepanker Tewari ◽  
Shelley C. Rankin ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Chicken Meat ◽  
Clinical Isolates ◽  
Retail Chicken Meat

scholarly journals Antimicrobial Effect of Essential Oils on Multidrug-Resistant Salmonella typhimurium in Chicken Fillets

2021 ◽  
Vol 41 (04) ◽  
pp. 545-551
Author(s):  
Alaa Eldin MA Morshdy
Keyword(s):  
Antimicrobial Resistance ◽  
Essential Oil ◽  
Essential Oils ◽  
Salmonella Typhimurium ◽  
Antimicrobial Effect ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Great Ability ◽  
16S Rna

Chicken meat, which could be a healthy and nutritionally food, is regrettably incriminated as a source of Salmonella typhimurium which has a great ability to cause human salmonellosis. This study aimed to assess the prevalence of multidrug-resistant S. typhimurium in chicken meat and the effects of essential oils on its viability. A total of 300 chicken meat and its products samples were streaked on XLD agar plates, which was followed by identification of the isolates based on biochemical and serological tests. Ten isolates were serotyped as S. typhimurium then assayed for susceptibility to 14 antimicrobials by the single diffusion method. Eight isolates (80%) showed multiple antimicrobial resistance (MAR) for 3 or more antimicrobials with MAR index of 0.4857 in average. Serotyped S. typhimurium strain with the highest antimicrobial resistance, confirmed by 16s RNA sequencing, was selected for studying the effects of thyme, oregano, and lemon essential oils with concentrations of 0.5, and 1% on its viability after inoculating into chicken fillets by intensity of 3.0×106 and on sensory traits of chicken fillets on 2nd, 4th, 6th, and 8th days of inoculation during cold storage (4ºC). All results showed a significant reduction of S. typhimurium counts with highest inhibition obtained using 1% lemon essential oil. The sensory properties of treated chicken fillets were improved by all used essential oils, compared to the control samples after 6th day, and 8th day of the storage period. The samples treated with 0.5, and 1% lemon essential oil revealed the highest improvement of sensory attributes. This study proved that the majority of S. typhimurium existing in chicken meat are Multidrug-resistant and have no negative effect on sensory traits, hence, posing a public health hazard. Natural essential oils have, also, great antimicrobial effect on S. typhimurium, thus it could replace chemical antimicrobials.

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