scholarly journals Chemical Compositions of the Volatile Oils and Antibacterial Screening of Solvent Extract from Downy Lavender

Foods ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 132 ◽  
Author(s):  
Chang Ha Park ◽  
Ye Eun Park ◽  
Hyeon Ji Yeo ◽  
Se Won Chun ◽  
Thanislas Bastin Baskar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Antimicrobial Activities ◽  
Aeromonas Salmonicida ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Significant Activity ◽  
Bacterial Strains

The discovery of a new species exhibiting more effective antibacterial properties is necessary because of the demand on Lavandula species, which continues to increase in a variety of industries. Lavandula pubescens might be a good alternative, as it exhibits strong antibacterial activity. In this study, the chemical composition of the essential oils from different organs (flowers, leaves, stems, and roots) of L. pubescens was identified using gas chromatography-mass spectrometry. Furthermore, the antimicrobial activities of different solvent extracts (methanol, ethanol, diethyl ether, hexane, and ethyl acetate) and different organ (flower, leaf, stem, and root) extracts of L. pubescens were evaluated. Only the ethyl acetate extracts of L. pubescens exhibited antibacterial activity against all bacterial strains tested, including Staphylococcus haemolyticus, Escherichia coli (KF 918342), Aeromonas hydrophila (KCTC 12487), E. coli (ATCC 35150), Cronobacter sakazakii (ATCC 29544), and Aeromonas salmonicida (KACC 15136). In particular, the extracts exhibited significant activity against S. haemolyticus. Ethyl acetate extract of the leaf exhibited the best activity against all bacterial strains. This study provides valuable information on the chemical compositions in essential oils and antimicrobial properties of L. pubescens.

scholarly journals Effects of Gender and Geographical Origin on the Chemical Composition and Antiradical Activity of Baccharis myriocephala and Baccharis trimera

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1433
Author(s):  
Suzana Struiving ◽  
Ana Carolina Mendes Hacke ◽  
Edésio Luiz Simionatto ◽  
Dilamara Riva Scharf ◽  
Cláudia Vargas Klimaczewski ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Ethyl Acetate ◽  
Essential Oils ◽  
Antiradical Activity ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Gas Chromatography Mass Spectrometry ◽  
Array Detector ◽  
Chemical Compositions ◽  
Baccharis Trimera

This study aimed to characterize and compare essential oils and ethyl acetate fractions obtained in basic and acidic conditions from both male and female Baccharis species (Baccharis myriocephala and Baccharis trimera) from two different Brazilian regions. Samples were characterized according to their chemical compositions and antiradical activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Principal component analysis (PCA) provided a clear separation regarding the chemical composition of essential oils from the samples obtained from different regions by using gas chromatography–mass spectrometry with flame-ionization detection (GC-MS-FID). PCA also revealed that gender and region of plant collections did not influence the chemical composition and antiradical activity of ethyl acetate fractions, which was corroborated with hierarchical cluster analysis (HCA) data. High performance liquid chromatography with diode-array detector (HPLC-DAD) identified significant quantities of flavonoids and phenolic acids in the fractions obtained in basic and acidic fractions, respectively. The obtained results clearly demonstrated that the geographical region of plant collection influenced the chemical composition of essential oils from the studied Baccharis species. Moreover, the obtained fractions were constituted by several antiradical compounds, which reinforced the usage of these species in folk medicine.

scholarly journals Chemical Indices and Antibacterial Properties of Some Essential Oils of Apiaceae and Lauraceae Spices in Southwest of Algeria

2019 ◽  
Author(s):  
Dalila Razni ◽  
Linda Rouisset ◽  
Elhassan Benyagoub
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Antibacterial Properties ◽  
Independent Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cinnamon Essential Oil ◽  
The One

This study is a part of the valorization of extract from three most commonly used Algerian spices, namely; caraway and cumin seeds and cinnamon bark. On the one hand, it aims at characterizing the chemical indices of extracted essential oils and evaluating the antibacterial activity of each essential oil by titration and disc diffusion method respectively. On the other hand, it attempts at evaluating the combined action of essential oils against four reference pathogenic bacterial strains, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis by well and Chabbert-type diffusion method. The essential oils obtained by the hydrodistillation method have a relatively average extraction about 1.43, 2.3 and 2.5%, respectively for caraway, cumin, and cinnamon. The acid index indicates the behavior and amount of free acids present in the essential oil, in which the acid and saponification indices of cinnamon essential oil indicate a value of 4.48 and 168.56 respectively. It can also inform us about the susceptibility of the oil to undergo alterations. The antibacterial activity results showed that cinnamon essential oil (EO) proved to be the most active against the tested bacterial strains; caraway EO was active against Enterococcus faecalis, and the antibacterial action of cumin EO was the lowest. However, the association of the extracted essential oils has a higher synergistic effect than the independent effect of each essential oil, in which the MIC value found was estimated at 10 to 20 (V/V), 40 to 50 (V/V) and 50 to 70 (V/V) respectively for cinnamon, cumin and caraway. The obtained results show that the response to the antibacterial activity varies according to the plant species used and the extract tested alone or in combination.

scholarly journals Comparison of Chemical Composition and Antibacterial Activity of Nigella sativa Seed Essential Oils Obtained by Different Extraction Methods

2008 ◽  
Vol 71 (12) ◽  
pp. 2475-2480 ◽  
Author(s):  
L. KOKOSKA ◽  
J. HAVLIK ◽  
I. VALTEROVA ◽  
H. SOVOVA ◽  
M. SAJFRTOVA ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Steam Distillation ◽  
Nigella Sativa ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Chemical Compositions ◽  
Gram Positive ◽  
Broth Microdilution Method

Nigella sativa L. seed essential oils obtained by hydrodistillation (HD), dry steam distillation (SD), steam distillation of crude oils obtained by solvent extraction (SE-SD), and supercritical fluid extraction (SFE-SD) were tested for their antibacterial activities, using the broth microdilution method and subsequently analyzed by gas chromatography and gas chromatography–mass spectrometry. The results showed that the essential oils tested differed markedly in their chemical compositions and antimicrobial activities. The oils obtained by HD and SD were dominated by p-cymene, whereas the major constituent identified in both volatile fractions obtained by SD of extracted oils was thymoquinone (ranging between 0.36 and 0.38 g/ml, whereas in oils obtained by HD and SD, it constituted only 0.03 and 0.05 g/ml, respectively). Both oils distilled directly from seeds showed lower antimicrobial activity (MICs ≥ 256 and 32 μg/ml for HD and SD, respectively) than those obtained by SE-SD and SFE-SD (MICs ≥ 4 μg/ml). All oil samples were significantly more active against gram-positive than against gram-negative bacteria. Thymoquinone exhibited potent growth-inhibiting activity against gram-positive bacteria, with MICs ranging from 8 to 64 μg/ml.

Antibacterial Activity of Rosmarinus officinalis and Lavandula angustifolia Essential Oils Against Selected Poultry Pathogenic Bacteria

2021 ◽  
Vol 1 (30) ◽  
pp. 49-57
Author(s):  
F.Moukhfi N.Chadli
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Rosmarinus Officinalis ◽  
Poultry Feed ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Lavandula Angustifolia

Abstract- Poultry is the host of many species of bacteria and the intestine is the privileged place of their colonization and their persistence. These bacteria are the cause of several cases of food poisoning in humans through the consumption of eggs or soiled chicken meat. In addition, these bacteria develop resistance to antibiotics that are adjusted to poultry feed as growth promoters. Essential oils are considered as important secondary metabolites for plant defense by their antimicrobial and antioxidant properties. These essential oils may be considered as a source of natural antimicrobials for the conservation of poultry food. The aim of our study is to isolate and identify bacterial strains isolated from poultry and to determine the antibacterial and antioxidant activities of Rosmarinus officinalis and Lavandula angustifolia essential oils on these bacterial strains.Essential oils of Moroccan Rosmarinus officinalis and Lavandula angustifolia were extracted by hydrodistillation. The identification of their chemical composition are performed by gas chromatography-mass spectrometry. Antimicrobial activity of extracted essential oils against Staphylococcus aureus, Clostridium perfringens, Escherichia coli and Salmonella entiritidis was evaluated by aromatogram test and Microdilution in a liquid medium. The identification of strains are performed by several test: Gram staining, Kligler test, Catalase test, test Mobility and Api Gallery 20E. The results show that essential oils tested have a considerable antibacterial activity against all isolated bacterial strains. Keywords: Poultry feed, Essential oils, Bacterial strains, Antibiotic resistance, Antimicrobial activity.

scholarly journals Chemical Composition, Antioxidant, and Antimicrobial Activities of the Essential Oils From Аrtemisia annua L. Growing Wild in Tajikistan

2020 ◽  
Vol 15 (5) ◽  
pp. 1934578X2092781
Author(s):  
Farukh S Sharopov ◽  
Aminjon Salimov ◽  
Sodik Numonov ◽  
Abduahad Safomuddin ◽  
Mahinur Bakri ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Hierarchical Cluster ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Aerial Parts ◽  
Antioxidant And Antimicrobial Activities

The aerial parts of Аrtemisia annua L. were collected from Varzob, Rudaki, and Hisor regions of Tajikistan. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry. The essential oils of aerial parts of А. annua were dominated by the monoterpenoids like camphor (32.5%-58.9%), 1,8-cineole (13.7%-17.8%), camphene (4.5%-8.4%), and α-pinene (1.9%-7.3%). Hierarchical cluster analysis of A. annua essential oils indicated the existence of 3 A. annua chemotypes: camphor/1,8-cineole, camphor, and artemisia ketone. The essential oils of А. annua show weak antioxidant activity and average antibacterial activity. In our opinion, the antibacterial activity of А. annua essential oils is related to the presence of 1,8-cineole. To our best knowledge, this is the first report concerning the chemical composition, chemotypic variation, antioxidant, and antimicrobial activities of the essential oils obtained from the aerial parts of А. annua, growing wild in Tajikistan.

scholarly journals Liquid and Vapor Phase of Four Conifer-Derived Essential Oils: Comparison of Chemical Compositions and Antimicrobial and Antioxidant Properties

2021 ◽  
Vol 14 (2) ◽  
pp. 134 ◽  
Author(s):  
Stefania Garzoli ◽  
Valentina Laghezza Masci ◽  
Valentina Caradonna ◽  
Antonio Tiezzi ◽  
Pierluigi Giacomello ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Essential Oils ◽  
Vapor Phase ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Abies Alba ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimal Bactericidal Concentration ◽  
Chemical Compositions ◽  
Bacterial Strains

In this study, the chemical composition of the vapor and liquid phase of Pinus cembra L., Pinus mugo Turra, Picea abies L., and Abies Alba M. needles essential oils (EOs) was investigated by Headspace-Gas Chromatography/Mass Spectrometry (HS-GC/MS). In the examined EOs, a total of twenty-eight components were identified, most of which belong to the monoterpenes family. α-Pinene (16.6–44.0%), β-pinene (7.5–44.7%), limonene (9.5–32.5%), and γ-terpinene (0.3–19.7%) were the most abundant components of the liquid phase. Such major compounds were also detected in the vapor phase of all EOs, and α-pinene reached higher relative percentages than in the liquid phase. Then, both the liquid and vapor phases were evaluated in terms of antibacterial activity against three Gram-negative bacteria (Escherichia coli, Pseudomonas fluorescens, and Acinetobacter bohemicus) and two Gram-positive bacteria (Kocuria marina and Bacillus cereus) using a microwell dilution assay, disc diffusion assay, and vapor phase test. The lowest Minimum Inhibitory Concentration (MIC) (13.28 mg/mL) and Minimal Bactericidal Concentration (MBC) (26.56 mg/mL) values, which correspond to the highest antibacterial activities, were reported for P. abies EO against A. bohemicus and for A. alba EO against A. bohemicus and B. cereus. The vapor phase of all the tested EOs was more active than liquid phase, showing the inhibition halos from 41.00 ± 10.15 mm to 80.00 ± 0.00 mm for three bacterial strains (A. bohemicus, K. marina, and B. cereus). Furthermore, antioxidant activities were also investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3- ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays, and a concentration-dependent antioxidant capacity for all EOs was found. P. mugo EO showed the best antioxidant activity than the other Pinaceae EOs. The four Pinaceae EOs could be further investigated for their promising antibacterial and antioxidant properties, and, in particular, α-pinene seems to have interesting possibilities for use as a novel natural antibacterial agent.

Composition and Antimicrobial Properties of Sardinian Juniperus Essential Oils against Foodborne Pathogens and Spoilage Microorganisms

2003 ◽  
Vol 66 (7) ◽  
pp. 1288-1291 ◽  
Author(s):  
SOFIA COSENTINO ◽  
ANDREA BARRA ◽  
BARBARA PISANO ◽  
MADDALENA CABIZZA ◽  
FILIPPO MARIA PIRISI ◽  
...  
Keyword(s):  
Essential Oils ◽  
Foodborne Pathogens ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Aflatoxin Contamination ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Microdilution Method ◽  
Ms Analysis ◽  
Broth Microdilution Method

In this work, the chemical compositions and antimicrobial properties of Juniperus essential oils and of their main components were determined. Five berry essential oils obtained from different species of Juniperus growing wild in Sardinia were analyzed. The components of the essential oils were identified by gas chromatography–mass spectrometry (GC-MS) analysis. The antimicrobial activities of the oils and their components against food spoilage and pathogenic microorganisms were determined by a broth microdilution method. The GC-MS analysis showed a certain variability in the concentrations of the main constituents of the oils. α-Pinene was largely predominant in the oils of the species J. phoenicea subsp. turbinata and J. oxycedrus. α-Pinene and myrcene constituted the bulk (67.56%) of the essential oil of J. communis. Significant quantitative differences were observed for myrcene, δ-3-carene, and d-germacrene. The results of the antimicrobial assay show that the oils of J. communis and J. oxycedrus failed to inhibit any of the microorganisms at the highest concentrations tested (MLC ≥ 900 μg/ml), while the oils extracted from J. turbinata specimens were active against fungi, particularly against a strain of Aspergillus flavus (an aflatoxin B1 producer). Of the single compounds tested, δ-3-carene was found to possess the broadest spectrum of activity and appeared to contribute significantly to the antifungal activity observed for J. turbinata oils. This activity may be helpful in the prevention of aflatoxin contamination for many foods.

scholarly journals Antifungal Activity and Composition of Essential Oils ofConyza canadensisHerbs and Roots

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Katalin Veres ◽  
Boglárka Csupor-Löffler ◽  
Andrea Lázár ◽  
Judit Hohmann
Keyword(s):  
Essential Oils ◽  
Antimicrobial Activities ◽  
Major Constituent ◽  
Chemical Compositions ◽  
Bacterial Strains ◽  
Strong Activity ◽  
Fungal Strains ◽  
Aerial Parts ◽  
Agar Disc ◽  
Main Component

Essential oils from herbs and roots ofConyza canadensis(horseweed), collected in Hungary, were obtained by hydrodistillation. The chemical compositions of the oils were analysed by combination of GC and GC/MS. The major constituent of the oil obtained from the aerial parts of horseweed was limonene (78%), while the main component of root oil was 2Z,8Z-matricaria ester. The antimicrobial activities of the oils were tested on Gram-positive (Enterococcus faecalis,Staphylococcus aureus, andStreptococcus pyogenes), Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria, reference fungal strains, and fungal strains isolated from patients (Candida,Cryptococcus,Trichophyton,Rhodotorula, andAspergillus) by agar disc diffusion and broth dilution methods. None of the oils showed any activity against the tested bacterial strains, but exhibited moderate-to-strong activity against all fungi with the only exception ofA. fumigatus. The highest zone of inhibition was observed in case ofCryptococcus neoformansandTrichophyton interdigitalis

scholarly journals Role of Direct Bioautographic Method for Detection of Antistaphylococcal Activity of Essential Oils

2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Györgyi Horváth ◽  
Noémi Jámbor ◽  
Erika Kocsis ◽  
Andrea Böszörményi ◽  
Έva Lemberkovics ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Agar Plate ◽  
Chemical Characterization ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Tea Tree ◽  
Antistaphylococcal Activity ◽  
Bioautographic Method

The aim of the present study was the chemical characterization of some traditionally used and therapeutically relevant essential oils (thyme, eucalyptus, cinnamon bark, clove, and tea tree) and the optimized microbiological investigation of the effect of these oils on clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA). The chemical composition of the oils was analyzed by TLC, and controlled by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The antibacterial effect was investigated using a TLC-bioautographic method. Antibacterial activity of thyme, clove and cinnamon oils, as well as their main components (thymol, carvacrol, eugenol, and cinnamic aldehyde) was observed against all the bacterial strains used in this study. The essential oils of eucalyptus and tea tree showed weak activity in the bioautographic system. On the whole, the antibacterial activity of the essential oils could be related to their most abundant components, but the effect of the minor components should also be taken into consideration. Direct bioautography is more cost-effective and better in comparison with traditional microbiological laboratory methods (e.g. disc-diffusion, agar-plate technique).

Chemical composition and anti-microbial analysis of Mentha arvensis L. and Thymus linearis Benth. essential oils of leaves

2021 ◽  
Vol 26 (5) ◽  
pp. 2893-2900
Author(s):  
SAJIDA BIBI ◽  
◽  
SHAHAB ALI ◽  
SHAHI DIN ◽  
IKRAM ULLAH ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Compositional Data ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Mentha Arvensis ◽  
Microbial Analysis ◽  
First Time

This study was performed to evaluate the chemical composition of the essential oils of Mentha arvensis and Thymus linearis and their antimicrobial activities. The complexity of the essential oils is a basic challenge for determining their reliable and accurate compositional data. Rapid advances in spectroscopic and chromatographic techniques have resolved this challenge to a large extent by examining essential oils. Essential oils were analyzed through Gas Chromatography-Mass Spectrometry (GC-MS) following extraction through steam distillation from their leaves for the first time in Miandam, District Swat, KP, Pakistan. The GC-MS analysis revealed 26 and 25 components in the essential oil of Mentha arvensis and Thymus linearis respectively. The major components were carvone (23.53%), P-Cymen-2-ol (20.35%), and caryophyllene oxide (18.81%) in Mentha arvensis but Thymus linearis has Thymol (2-isopropyl-5-methyl phenol) (40%), O-Cymene (2-Isopropyltoluene) (14.95%) and beta-bisbolene (12.54%). The essential oils of both plants showed bactericidal activities against five different bacterial strains (i.e. Escherichia coli, Staphylococcus aureus, Klesiella pneumonia, Bacillus subtilis, and Pseudomonas aeruginosa) during disc diffusion method and therefore it is suggested that they may be used as a natural antiseptic and could play important roles in food and pharmaceutical industry.

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