Characterization and Antimicrobial Activity of Biodegradable Active Packaging Enriched with Clove and Thyme Essential Oil for Food Packaging Application

Bioactive packaging contains natural antimicrobial agents, which inhibit the growth of microorganisms and increase the food shelf life. Solvent casting method was used to prepare the Poly (lactide)-Poly (butylene adipate-co-terephthalate) (PLA-PBAT) film incorporated with the thyme oil and clove oil in various concentrations (1 wt%, 5 wt% and 10 wt%). The clove oil composite films depicted less green and more yellow as compared to thyme oil composite films. Clove oil composite film has shown an 80% increase in the UV blocking efficiency. The tensile strength (TS) of thyme oil and clove oil composite film decreases from 1.35 MPs (control film) to 0.96 MPa and 0.79, respectively. A complete killing of S. aureus that is a reduction from 6.5 log CFU/mL to 0 log CFU/mL was observed on the 10 wt% clove oil incorporated composite film. Clove oil and thyme oil composite film had inhibited E. coli biofilm by 93.43% and 82.30%, respectively. Clove oil composite film had exhibited UV blocking properties, strong antimicrobial activity and has high potential to be used as an active food packaging.

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Fabrication of Copper Sulfide Nanoparticles and Limonene Incorporated Pullulan/Carrageenan-Based Film with Improved Mechanical and Antibacterial Properties

Polymers ◽

10.3390/polym12112665 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2665

Author(s):

Swarup Roy ◽

Jong-Whan Rhim

Keyword(s):

Composite Film ◽

Copper Sulfide ◽

Food Packaging ◽

Pathogenic Bacteria ◽

Composite Films ◽

Antibacterial Properties ◽

Barrier Properties ◽

Food Borne ◽

Active Food Packaging ◽

Thermal Stability Of

Edible biopolymer (pullulan/carrageenan) based functional composite films were fabricated by the addition of copper sulfide nanoparticles (CuSNP) and D-limonene (DL). The DL and CuSNP were compatible with the pullulan/carrageenan biopolymer matrix. The addition of CuSNP significantly increased the UV-blocking properties without substantially reducing the transparency of the film. The addition of CuSNP improved the film’s tensile strength by 10%; however, the DL addition did not significantly influence the strength, while the combined addition of CuSNP and DL increased the strength by 15%. The addition of the fillers did not significantly affect the thermal stability of the film, but the water vapor barrier property was slightly improved. There was no significant change in the moisture content and hydrophobicity of the composite film. Besides, the composite film showed some antimicrobial activity against food-borne pathogenic bacteria. The fabricated pullulan/carrageenan-based film with antimicrobial and UV-barrier properties is likely to be used in active food packaging applications.

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Efficacy of Antimicrobial Agents for Food Contact Applications: Biological Activity, Incorporation into Packaging, and Assessment Methods: A Review

Journal of Food Protection ◽

10.4315/0362-028x.jfp-17-509 ◽

2018 ◽

Vol 81 (7) ◽

pp. 1142-1156 ◽

Cited By ~ 10

Author(s):

AMIN MOUSAVI KHANEGHAH ◽

SEYED MOHAMMAD BAGHER HASHEMI ◽

ISMAIL EŞ ◽

DANIELA FRACASSETTI ◽

SARA LIMBO

Keyword(s):

Antimicrobial Activity ◽

Food Packaging ◽

Antimicrobial Agents ◽

Critical Issue ◽

Active Packaging ◽

Assessment Methods ◽

Packaging Materials ◽

Food Contact ◽

Inorganic Substances ◽

Active Food Packaging

ABSTRACTInterest in the utilization of antimicrobial active packaging for food products has increased in recent years. Antimicrobial active packaging involves the incorporation of antimicrobial compounds into packaging materials, with the aim of maintaining or extending food quality and shelf life. Plant extracts, essential oils, organic acids, bacteriocins, inorganic substances, enzymes, and proteins are used as antimicrobial agents in active packaging. Evaluation of the antimicrobial activity of packaging materials using different methods has become a critical issue for both food safety and the commercial utilization of such packaging technology. This article reviews the different types of antimicrobial agents used for active food packaging materials, the main incorporation techniques, and the assessment methods used to examine the antimicrobial activity of packaging materials, taking into account their safety as food contact materials.

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Incorporation of Poly(Itaconic Acid) with Quaternized Thiazole Groups on Gelatin-Based Films for Antimicrobial-Active Food Packaging

Polymers ◽

10.3390/polym13020200 ◽

2021 ◽

Vol 13 (2) ◽

pp. 200

Author(s):

Celeste Cottet ◽

Andrés G. Salvay ◽

Mercedes A. Peltzer ◽

Marta Fernández-García

Keyword(s):

Antimicrobial Activity ◽

Itaconic Acid ◽

Food Packaging ◽

Crosslinking Agent ◽

Radical Scavenging ◽

Proton Nuclear Magnetic Resonance ◽

Esterification Reaction ◽

Alkylation Reaction ◽

Vapor Permeability ◽

Active Food Packaging

Poly(itaconic acid) (PIA) was synthesized via conventional radical polymerization. Then, functionalization of PIA was carried out by an esterification reaction with the heterocyclic groups of 1,3-thiazole and posterior quaternization by N-alkylation reaction with iodomethane. The modifications were confirmed by Fourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H-NMR), as well as ζ-potential measurements. Their antimicrobial activity was tested against different Gram-negative and Gram-positive bacteria. After characterization, the resulting polymers were incorporated into gelatin with oxidized starch and glycerol as film adjuvants, and dopamine as crosslinking agent, to develop antimicrobial-active films. The addition of quaternized polymers not only improved the mechanical properties of gelatin formulations, but also decreased the solution absorption capacity during the swelling process. However, the incorporation of synthesized polymers increased the deformation at break values and the water vapor permeability of films. The antioxidant capacity of films was confirmed by radical scavenging ability and, additionally, those films exhibited antimicrobial activity. Therefore, these films can be considered as good candidates for active packaging, ensuring a constant concentration of the active compound on the surface of the food, increasing products’ shelf-life and reducing the environmental impact generated by plastics of petrochemical origin.

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Enhanced functional properties of biodegradable Polyvinyl alcohol /carboxymethyl cellulose (PVA/CMC) composite films reinforced with L-alanine surface modified CuO nanorods

10.21203/rs.3.rs-918116/v1 ◽

2021 ◽

Author(s):

Yamanappagouda Amaregouda ◽

Kantharaju Kamanna ◽

Tilak Gasti ◽

Vijay Kumbar

Keyword(s):

Polyvinyl Alcohol ◽

Carboxymethyl Cellulose ◽

Food Packaging ◽

Composite Films ◽

Nanocomposite Films ◽

Soil Burial ◽

High Antibacterial Activity ◽

Active Food Packaging ◽

Surface Modified ◽

Cuo Nanorods

Abstract Herein, we described novel biogenic preparation of the CuO nanorods and its surface modification with L-alanine amino acid accelerated by microwave irradiation. The effect of surface functionalized CuO nanorods on the polyvinyl alcohol/carboxymethyl cellulose film physico-mechanical properties were investigated through various characterization techniques. The tensile strength was improved from 28.58 ± 0.73 MPa to 43.40 ± 0.93 MPa, UV shielding ability and barrier to the water vapors were highly enhanced when PVA/CMC matrices filled with 8 wt% of CuO-L-alanine. In addition, the prepared films exhibited acceptable overall migration limit and readily undergoes soil burial degradation. Nevertheless, CuO-L-alanine incorporated films showed potent antioxidant activity against DPPH radicals and had high antibacterial activity against Staphylococcus aureus and Escherichia coli, and antifungal activity against Candida albicans and Candida tropicalis. Furthermore, the nanocomposite films showed negligible cytotoxic effect on HEK293 and Caco-2 cell lines. In these contexts, the developed nanocomposite films can be implementing as an active food packaging material.

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Effect of Poly (Lactic–Co–Glycolic Acid) (75:25)/ Hydroxyapatite Composite on Chondrocyte Culture for Tissue Reconstruction

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.353 ◽

2007 ◽

Vol 342-343 ◽

pp. 353-356 ◽

Cited By ~ 2

Author(s):

Jung Bok Lee ◽

Seong Mi Yu ◽

Sang Gil Lee ◽

Jae Bong Choi ◽

Jeong Koo Kim

Keyword(s):

Composite Film ◽

Cell Attachment ◽

Composite Films ◽

Contact Angle Measurement ◽

Angle Measurement ◽

Control Group ◽

Cellular Attachment ◽

Control Film ◽

Content Ratio

PLGA (75:25)/hydroxyapatite (HA) composite films were fabricated by solvent-casting method to investigate the effect of various hydroxyapatite content ratio to the PLGA film for cellular attachment and proliferation. Mechanical property of the composite film was characterized by tensile test. The ultimate tensile strength of 10% HA content film was two folds higher than control group. The surface of the film was characterized by contact angle measurement. The PLGA/HA composite film was more hydrophilic than control film. In vitro chondrocyte responses to the composite films were measured by cellular attachment and proliferation test. The attached and proliferated cells were significantly higher on PLGA/HA (10%) composite film than control group (1.44 times higher in attachment test and 1.31 times higher for 6th-day at culture in proliferation assaying, p<0.05). Base on these finding, the PLGA/HA (10%) composite was effective for the cell attachment for the initial stage of cultivation and cell proliferation.

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Role of ZnO Nanoparticles for improvement of Antibacterial Activity in Food Packaging

Asian Journal of Pharmaceutical Research ◽

10.52711/2231-5691.2021.00024 ◽

2021 ◽

pp. 128-131

Author(s):

Saira Sehar ◽

Amiza Amiza ◽

I. H Khan

Keyword(s):

Antimicrobial Activity ◽

Particle Size ◽

Surface Area ◽

Zno Nanoparticles ◽

Food Packaging ◽

Antimicrobial Agents ◽

Uv Light ◽

Zno Nps ◽

Long Time

Nanotechnology advancement leads to development of antimicrobial agents like ZnO nanoparticles. These nanoparticle have their main applications in food packaging. when these nanoparticles incorporate into the food surface, it will kill all bacterias residing on the surface and food become free of bacteria. In this way, food can be stored for a long time because its shelf life is improved. Antimicrobial activity of ZnO nanoparticles can be improved by increasing surface area, reducing particle size and large concentration of ZnO –NPS. Antimicrobial activity increases by increasing intensity of UV light. As UV light fall on ZnO nanoparticles, it increases ZnO surface area and hence anrtimicrobial activity will be increased. Exact mechanism of Antimicrobial activity is still unknown but some processes have been presented.

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Investigation of Ultrasonic Treatment on Physicochemical, Structural and Morphological Properties of Sodium Alginate/AgNPs/Apple Polyphenol Films and Its Preservation Effect on Strawberry

Polymers ◽

10.3390/polym12092096 ◽

2020 ◽

Vol 12 (9) ◽

pp. 2096

Author(s):

Wenting Lan ◽

Siying Li ◽

Shiti Shama ◽

Yuqing Zhao ◽

Dur E. Sameen ◽

...

Keyword(s):

Antibacterial Activity ◽

Composite Film ◽

Sodium Alginate ◽

Food Packaging ◽

Base Material ◽

Morphological Properties ◽

Vapor Permeability ◽

Sem Images ◽

Active Food Packaging ◽

Ultrasonic Time

An antibacterial and anti-oxidation composite film was prepared by a casting method using sodium alginate (SA) and apple polyphenols (APPs) as the base material and glycerol as the plasticizer. Silver nanoparticles (AgNPs) were deposited by ultrasonic-assisted electrospray method. The degree of influence of the addition ratio of SA and AgNPs and different ultrasonic time on the mechanical properties, barrier properties, optical properties, and hydrophilicity of the composite film was explored. The composite films were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results showed that the SA: AgNPs ratio of 7:3 and the ultrasonic time for 30 min have the best comprehensive performance, and SA/AgNPs/APP films showed the lowest water vapor permeability value of 0.75 × 10−11 g/m·s·Pa. The composite film has good strength and softness, with tensile strength (TS) and elongation at break (E) at 23.94 MPa and 29.18%, respectively. SEM images showed that the surface of the composite film was smooth and the AgNPs’ distribution was uniform. The composite film showed broad antibacterial activity, and the antibacterial activity of Escherichia coli (92.01%) was higher than that of Staphylococcus aureus (91.26%). However, due to the addition of APP, its antioxidant activity can reach 98.39%, which has a synergistic effect on antibacterial activity. For strawberry as a model, the results showed that this composite film can prolong the shelf life of strawberries for about 8 days at 4 °C, effectively maintaining their storage quality. Compared with the commonly used PE(Polyethylene film) film on the market, it has a greater fresh-keeping effect and can be used as an active food packaging material.

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Antimicrobial Activity of Allyl Isothiocyanate Used To Coat Biodegradable Composite Films as Affected by Storage and Handling Conditions†

Journal of Food Protection ◽

10.4315/0362-028x.jfp-12-234 ◽

2012 ◽

Vol 75 (12) ◽

pp. 2234-2237 ◽

Cited By ~ 8

Author(s):

WEILI LI ◽

LINSHU LIU ◽

TONY Z. JIN

Keyword(s):

Antimicrobial Activity ◽

Polylactic Acid ◽

Food Packaging ◽

Storage Time ◽

Storage Temperature ◽

Composite Films ◽

Antimicrobial Properties ◽

Allyl Isothiocyanate ◽

Microbial Reduction ◽

Biodegradable Composite

We evaluated the effects of storage and handling conditions on the antimicrobial activity of biodegradable composite films (polylactic acid and sugar beet pulp) coated with allyl isothiocyanate (AIT). Polylactic acid and chitosan were incorporated with AIT and used to coat one side of the film. The films were subjected to different storage conditions (storage time, storage temperature, and packed or unpacked) and handling conditions (washing, abrasion, and air blowing), and the antimicrobial activity of the films against Salmonella Stanley in tryptic soy broth was determined. The films (8.16 μl of AIT per cm2 of surface area) significantly (P < 0.05) inhibited the growth of Salmonella during 24 h of incubation at 22°C, while the populations of Salmonella in controls increased from ca. 4 to over 8 log CFU/ml, indicating a minimum inactivation of 4 log CFU/ml on films in comparison to the growth on controls. Statistical analyses indicated that storage time, storage temperature, and surface abrasion affected the antimicrobial activity of the films significantly (P < 0.05). However, the differences in microbial reduction between those conditions were less than 0.5 log cycle. The results suggest that the films' antimicrobial properties are stable under practical storage and handling conditions and that these antimicrobial films have potential applications in food packaging.

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Physical Properties and Antimicrobial Activity of a Poly(Lactic Acid)/Poly(Trimethylene Carbonate) Film Incorporated with Thymol

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.884-885.481 ◽

2014 ◽

Vol 884-885 ◽

pp. 481-484 ◽

Cited By ~ 2

Author(s):

Yan Wu ◽

Ming Wei Yuan ◽

Ji Yi Yang ◽

Yu Yue Qin ◽

Ming Long Yuan ◽

...

Keyword(s):

Antimicrobial Activity ◽

Lactic Acid ◽

Water Vapor ◽

Composite Film ◽

Food Packaging ◽

Water Vapor Permeability ◽

Antimicrobial Activities ◽

Poly Lactic Acid ◽

Vapor Permeability ◽

Trimethylene Carbonate

Thymol (TH), which has antimicrobial effect on many food pathogens, was incorporated as antimicrobial agent into composite poly (lactic acid)/poly (trimethylene carbonate)(PLA-PTMC) films. Antimicrobial active films based on PLA-PTMC were prepared by incorporating thymol at five different concentrations: 0, 3, 6, 9 and 12 %(w/w). The mechanical characterization, water vapor permeability (WVP), and antimicrobial activity of all formulations composite film were carried out. A decrease in elastic modulus was obtained for the active composite film compared with neat PLA-PTMC film. The presence of thymol decreased water vapor permeability, with a significant antimicrobial activity. Antimicrobial activities of films were tested against Escherichia coli, Staphylococcus aurous, Listeria, Bacillus subtilis, and Salmonella. Increasing amount of the thymol in the film caused a significant increase in inhibitory zones. These results suggest that thymol incorporated PLA-PTMC films have a prospectively potential in antimicrobial food packaging.

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