scholarly journals Effect of Roasting and Brewing on the Antioxidant and Antiproliferative Activities of Tartary Buckwheat

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1331
Author(s):  
Ji-yeon Ryu ◽  
Yoonseong Choi ◽  
Kun-Hwa Hong ◽  
Yong Suk Chung ◽  
Somi Kim Cho
Keyword(s):  
Breast Cancer Cells ◽  
Room Temperature ◽  
Tartary Buckwheat ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Antiproliferative Effects ◽  
Long Time ◽  
Short Time ◽  
Antiproliferative Activities

We evaluated the effect of the roasting and brewing conditions of Tartary buckwheat (TB), which is widely used in infusion teas, on its antioxidant and antiproliferative activities in vitro. TB was roasted at 210 °C for 10 min and brewed at a high temperature for a short time (HTST; 85–90 °C, 3 min) or at room temperature for a long time (RTLT; 25–30 °C, 24 h). Roasted TB (RTB) tea brewed at RTLT had the highest total polyphenol content (TPC) and total flavonoid content (TFC) among the four TB teas for different roasting and brewing conditions. Moreover, RTB brewed at RTLT showed the greatest 2,2-diphenyl-1-picrylhydrazyl-, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)-, and alkyl-scavenging activities. The TB tea brewed at RTLT had higher Fe2+-chelating activity than that brewed at HTST, irrespective of roasting. Moreover, RTB tea brewed at RTLT inhibited the proliferation of human pancreatic and breast cancer cells. Overall, RTB-RTLT displayed the largest effect on antioxidant and antiproliferative effects. Finally, rutin was found to possess the most pronounced effect on the antioxidant and antiproliferative activities of the TB teas. These results indicate that the antioxidant and antiproliferative activities of RTB are enhanced by RTLT brewing.

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

Antioxidant and Antiproliferative Activities of Crossostephium chinensis (L.) Makino

2009 ◽  
Vol 37 (04) ◽  
pp. 797-814 ◽  
Author(s):  
Tien-Ning Chang ◽  
Guan-Jhong Huang ◽  
Yu-Lin Ho ◽  
Shyh-Shyun Huang ◽  
Heng-Yuan Chang ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Bioactive Compound ◽  
Natural Antioxidants ◽  
Dependent Manner ◽  
Total Polyphenol ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2 ◽  
Antiproliferative Activities

Crossostephium chinensis (L.) (CC) Makino is a common traditional Chinese medicinal plant used to dehumidify and cure rheumatism and arthralgia. The water and methanol extracts of C. chinensis (CCW and CCM) were evaluated for their antioxidant and antiproliferative activities. The antioxidant activities of CC were evaluated by using ABTS radical scavenging, DPPH radical scavenging, nitric oxide scavenging and superoxide scavenging methods. Iron chelating activity, lipid peroxidation, total polyphenol contents, total flavonoid contents and total flavonol contents were also detected. In all the tested models, both CCW and CCM showed their ability to scavenge the free radicals in a does-dependent manner. CCW had higher antioxidant and antiproliferative activities than CCM. In LC-MS-MS analysis, the chromatograms of CCW with good antioxidant activities were established. Rutin might be an important bioactive compound in CCW. The antiproliferative activities of CCW and CCM were also studied in vitro by using human hepatoma HepG2 cells. CCW exhibited good antiproliferative activity. These results indicated that CCW might be used as a potential source of natural antioxidants and as an anti-tumor agent.

scholarly journals BetaSweet carrot extracts have antioxidant activity and in vitro antiproliferative effects against breast cancer cells

2019 ◽  
Vol 62 ◽  
pp. 103552 ◽  
Author(s):  
G.K. Jayaprakasha ◽  
K.N. Chidambara Murthy ◽  
Federica Pellati ◽  
Bhimanagouda S. Patil
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Antiproliferative Effects

scholarly journals Micropropagation of Rare Scutellaria Havanensis Jacq. And Preliminary Studies on Antioxidant Capacity and Anti-cancer Potential

2021 ◽  
Author(s):  
Lani Irvin ◽  
Yarelia Zavala Ortiz ◽  
Kamila Rivera Rivera ◽  
Brajesh Nanda Vaidya ◽  
Samantha H Sherman ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
In Vitro Propagation ◽  
Adventitious Shoots ◽  
Colorectal Cancer Cell Line ◽  
Dependent Manner ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Total Polyphenol ◽  
Bud Induction

We report the development of in vitro propagation protocols through adventitious shoot induction pathway for a rare and medicinal Scutellaria havanensis. In vitro propagation studies using nodal explants showed MS medium supplemented with 10µM 6-Benzylaminopurine induced highest number of adventitious shoots in a time dependent manner. A ten - day incubation was optimum for shoot bud induction as longer exposures resulted in hyperhydricity of the explants and shoots induced. We also report preliminary evidence of Agrobacterium tumefaciens EHA105 - mediated gene transfer transiently expressing of green fluorescent protein in this species. Transformation studies exhibited amenability of various explant tissues, internode being the most receptive. As the plant has medicinal value, research was carried out to evaluate its potential antioxidant capacity and the efficacy of methanolic leaf extracts in curbing the viability of human colorectal cancer cell line HCT116. Comparative total polyphenol, and flavonoid content measurement of fresh and air dried leaf extract revealed that the fresh leaf extracts contain higher total polyphenol and flavonoid content. The HCT 116 cell viability assessed by colorimetric assay using a 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide, showed a steady growth inhibition after 24 hours of incubation. Scanning Electron Microscopy of leaf surface revealed high density of glandular and non-glandular trichomes. This research provides basis for the conservation of this rare plant and future phytochemical screening and clinical research.

scholarly journals Green Synthesis of New Pyrrolo [1,2-a] quinoxalines as Antiproliferative Agents in GPER-expressing Breast Cancer Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Gabriele Carullo ◽  
Sarah Mazzotta ◽  
Francesca Giordano ◽  
Francesca Aiello
Keyword(s):  
Anticancer Agents ◽  
Biological Properties ◽  
Antiproliferative Agents ◽  
Antiproliferative Effects ◽  
Anticancer Properties ◽  
Small Set ◽  
Acetyl Group ◽  
High Yields ◽  
Short Time

4,5-Dihydropyrrolo [1,2-a]quinoxalines are interesting druggable scaffolds, with multifaceted biological properties, including anticancer properties targeting the G protein-coupled estrogen receptor 1 (GPER). In this work, the synthesis and preliminary antiproliferative activity of a small set of new 4,5-dihydropyrrolo[1,2-a]quinoxalines (18-20) and pyrrolo[1,2-a]quinoxalines (21, 22) has been reported, inspired by known antiproliferative agents (G-1, G-15, and G-36). The synthesis of the pyrroloquinoxalinic core was employed following the Pictet–Spengler reaction, using the surfactant p-dodecylbenzene sulphonic acid (p-DBSA), as catalyst. It demonstrated efficiency in the catalysis of the 4-phenylpyrrole [1,2-a] quinoxaline type compound formation in mild solvents such as water, ethanol, and hydroalcoholic solutions. In addition, the reactions proceeded in a short time (between 15 and 120 minutes) at room temperature and with high yields. The in vitro MTT assays showed that the presence of isopropyl groups furnished promising antiproliferative compounds. Although, the acetyl group provided also antiproliferative effects, breaking down its responsibility in the GPER transactivation. Nevertheless, it is possible to conclude that the 4,5-dihydropyrrolo[1,2-a]quinoxalines remain a feasible scaffold to develop anticancer agents against GPER-expressing cells.

scholarly journals Influence of plant growth regulators on the rapid propagation buds of Dendrobium officinale Kimura et Migo

2017 ◽  
Vol 1 (T2) ◽  
pp. 29-38
Author(s):  
Diem Thi Le ◽  
Mai Thi Bach Vo
Keyword(s):  
Functional Foods ◽  
Culture Medium ◽  
High Efficiency ◽  
Nodal Explants ◽  
Dendrobium Officinale ◽  
Ms Medium ◽  
The World ◽  
Long Time ◽  
Short Time

Dendrobium officinale Kimura et Migo has been known for a long time as a precious orchid, which is used for medicine and functional foods that are widely commercialized in the world. The nodal explants could be obtained in high efficiency, good quality and uniform seedlings on multiplication vegetative in vitro. They can be propagated in large quantities in a short time. The studied results showed that the nodal explants grew on MS medium + 10 % coconut milk + 25 g sucrose + 0.5 mg/L BA + 6 g agar/ liter create high buds. This buds were used to A B 1 cm 1,5 cm create bud clusters with the aim of improving in vitro vegetative. The best bud culture medium for the formation of clusters was MS + BA 2.0 mg/L + NAA 0.4 mg/L; MS + kinetin 1.5 mg/L + NAA 0.3 mg/L; MS + TDZ 2.0 mg/L + NAA 0.4 mg/L; MS + adenine 1.5 mg/L + NAA 0.3 mg/L. However, the addition of kinetin or TDZ to the culture medium needed a steps to extend the bud subculture after 45 days in culture. The best medium for the rooting is MS + NAA 0.5 mg/L.

scholarly journals Antioxidants properties of chocolates sold in Peru

2018 ◽  
Vol 67 (4) ◽  
pp. 479-485
Author(s):  
Carmen Adela Orihuela-Rivera ◽  
Gabriela Cristina Chire ◽  
María Rosario Calixto-Cotos
Keyword(s):  
Reducing Power ◽  
Large Degree ◽  
In Vitro Models ◽  
Thermal Processes ◽  
Flavonoid Content ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Anti Oxidant ◽  
Different Levels

The goals of this paper were to evaluate total polyphenol content (TPC), total flavonoid content (TFC), anti-oxidant capacity (AC) and reducing power (RP) of eight dark chocolates that are sold in Peru. Imported and domestic chocolate samples containing between 50% and 74% cacao were defatted. Defatted chocolate (DCh) was separated and treated to extract phenolic compounds. The TPC and AC were determined by using the Folin-Ciocalteu reagent and two in vitro models based on the free-radical capturing capacity DPPH and ABTS. The RP was evaluated by using the potassium pherricyanide method; in the TFCs assessments, catechin was used as the standard. Chocolate TPC varied between 1.69 ± 0.02 and 5.39 ± 0.17 mg gallic acid/g chocolate and AC (DPPH-IC50) varied between 52.97 ± 1.77 and 158.67 ± 2.04 μg/ml extract, and by means of ABTS the values were between 12.01 ± 0.18 and 32.74 ± 0.49 μmol TEAC/g chocolate. Chocolates with 71% and 72% cacao showed a greater antioxidant capacity, which was confirmed by the RP test. Chocolates showed different levels of TPC, TFC, AC and RP, depended on a large degree of the cacao percentage. However, in some cases, there was no direct relationship among results, most likely due to different technological and thermal processes as well as different biological nature of cacao beans.

Antioxidant, Anti-inflammatory, and Antiproliferative Activities of Taxillus sutchuenensis

2012 ◽  
Vol 40 (02) ◽  
pp. 335-348 ◽  
Author(s):  
Chia-Yu Liu ◽  
Ying-Chih Lin ◽  
Jeng-Shyan Deng ◽  
Jung-Chun Liao ◽  
Wen-Huang Peng ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Radical Scavenging ◽  
A549 Cells ◽  
Raw264.7 Cells ◽  
Total Phenolic ◽  
No Production ◽  
Flavonoid Content ◽  
Anti Inflammatory ◽  
Antiproliferative Activities

Inflammation is related to several chronic diseases, including cancer and atherosclerosis. Taxillus sutchuenensis (Lecomte) Danser is a special folk medicinal plant in Taiwan. The aim of this study was to evaluate the antioxidant, anti-inflammatory, and antiproliferative activities of the aqueouse-thanol extract from T. sutchuenensis (AETS) and its fractions. TEAC, DPPH radicals, total phenolic compounds, total flavonoid content, inhibition of NO production in LPS-induced RAW264.7 cells, and inhibition of cancer cell proliferation were tested. Among all fractions, the ethyl-acetate (EA) fraction showed the highest TEAC and DPPH radical scavenging activities. The EA fraction also had the highest polyphenol and flavonoid content. The EA fractions also decreased LPS-induced NO production and the expression of iNOS and COX-2 in RAW264.7 cells. The antiproliferative activities of the aqueous/ethanol extract and fractions were studied in vitro using A549 cells, and the results were consistent with their antioxidant capacities. EA fractions had the highest antiproliferative activity with an IC50 of 454.38 ± 1.48 μg/ml. Quercetin also had antioxidant, anti-inflammatory, and antiproliferative activities. Quercetin might be an important bioactive compound in T. sutchuenensis. The experimental data indicated that T. sutchuenensis is a potent antioxidant medicinal plant, and such efficacy may be mainly attributed to its polyphenolic compounds.

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