scholarly journals Indices for the Assessment of Glycoalkaloids in Potato Tubers Based on Surface Color and Chlorophyll Content

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 107
Author(s):  
Shimeles Tilahun ◽  
Hee Sung An ◽  
Tifsehit Solomon ◽  
Min Woo Baek ◽  
Han Ryul Choi ◽  
...  
Keyword(s):  
High Performance ◽  
Potato Cultivars ◽  
Gas Content ◽  
Potato Tubers ◽  
Surface Color ◽  
Toxic Level ◽  
Laboratory Equipment ◽  
Chl A ◽  
Skilled Personnel ◽  
Ga Content

Glycoalkaloids (GAs) are toxic to humans at higher concentrations. However, studies also suggest the health benefits of GAs depending on the dose and conditions of use. Methods that have been used to determine GA content in potato tubers are destructive and time-consuming and require skilled personnel and high-performance laboratory equipment. We conducted this study to develop indices for the prediction of the level of total GAs in potato tubers at different greening stages based on surface color readings and chlorophyll (Chl) development. Color values (Hunter L*, a*, b*, a*/b*), Chls (Chl a, Chl b, and total Chls) and GA (α-solanine, α-chaconine, and total GAs) content were measured from tubers of ‘Atlantic’ and ‘Trent’ potato cultivars at three-week intervals in up to six greening stages during the storage at room conditions (22 °C, 12-h shift of light-dark cycles). The results have revealed that greening, Chls, and GA content significantly increased for the two cultivars as the stage proceeded. The toxic level of GAs (>200 mg kg−1 FW) was accumulated at the late greening stages, accompanied by the highest Chl content. Finally, indices were developed based on surface color and Chl content for estimation of the safe GA levels for the consumption of the two commercially and commonly used potato cultivars. Moreover, the developed indices could be used as basic information to adapt to other potato cultivars.

scholarly journals Anthocyanin Accumulation during Potato Tuber Development

1997 ◽  
Vol 122 (1) ◽  
pp. 20-23 ◽  
Author(s):  
Chen-Yi Hung ◽  
John R. Murray ◽  
Sarah M. Ohmann ◽  
Cindy B.S. Tong
Keyword(s):  
High Performance ◽  
Anthocyanin Content ◽  
Mrna Levels ◽  
Biosynthetic Enzyme ◽  
Potato Tubers ◽  
Tuber Development ◽  
Surface Color ◽  
Dihydroflavonol Reductase ◽  
Anthocyanin Concentration ◽  
Tuber Periderm

The color of red potato tubers is due to an accumulation of anthocyanins in periderm and peripheral cortex tissues. The objective of this study was to characterize changes in anthocyanin content and tuber surface color during tuber development. Using the red tuber-producing potato (Solanum tuberosum L.) cultivar Norland, we observed that chroma (intensity of redness) and anthocyanin content per unit of surface area of greenhouse-grown tubers decreased as tuber weight increased. There was no increase in hue (tint) during the same developmental periods. Using high-performance liquid chromatography (HPLC), we determined that pelargonidin and peonidin are the major anthocyanidins (aglycones of anthocyanins) in the tuber periderm. Northern blot analyses indicated that steady-state mRNA levels of dihydroflavonol reductase (DFR), an anthocyanin biosynthetic enzyme, continued throughout tuber development. These results suggest that anthocyanins are synthesized throughout tuber development, and that cell division and/or enlargement contribute to a decline in chroma and anthocyanin concentration.

scholarly journals Phytoplankton community composition in the south-eastern Black Sea determined with pigments measured by HPLC-CHEMTAX analyses and microscopy cell counts

2014 ◽  
Vol 95 (1) ◽  
pp. 35-52 ◽  
Author(s):  
Ertugrul Agirbas ◽  
Ali Muzaffer Feyzioglu ◽  
Ulgen Kopuz ◽  
Carole A. Llewellyn
Keyword(s):  
Black Sea ◽  
High Performance ◽  
Phytoplankton Community ◽  
Phytoplankton Community Structure ◽  
The South ◽  
Coastal Station ◽  
Chl A ◽  
South Eastern ◽  
Cell Counts ◽  
Offshore Station

The phytoplankton community structure and abundance in the south-eastern Black Sea was measured from February to December 2009 using and comparing high performance liquid chromatography pigment and microscopy analyses. The phytoplankton community was characterized by diatoms, dinoflagellates and coccolithophores, as revealed by both techniques. Fucoxanthin, diadinoxanthin, peridinin and 19′-hexanoyloxyfucoxanthin were the main accessory pigments showing significant correlation with diatom-C r2 = 0.56–0.71, P < 0.05), diatom-C (r2 = 0.85–0.91, P < 0.001), dinoflagellate-C (r2 = 0.39–0.88, P < 0.05) and coccolithophore-C (r2 = 0.80–0.71, P < 0.05), respectively. Microscopy counts indicated a total of 89 species, 71% of which were dinoflagellates, 23% were diatoms and 6% other species (mainly coccolithophores). Pigment-CHEMTAX analysis also indicated the presence of pico- and nanoplankton. Phytoplankton carbon (phyto-C) concentrations were highest in the upper water column, whereas chlorophyll-a (Chl-a) showed a deep maximum. Average phyto-C was higher at the coastal station (291 ± 66 µg l−1) than at the offshore station (258 ± 35 µg l−1), not statistically different (P > 0.05). The coastal station also had higher Chl-a concentrations (0.52–3.83 µg l−1) compared to the offshore station (0.63–2.55 µg l−1), not significant (P > 0.05). Our results are consistent with other studies and indicate that the southern Black Sea is shifting towards mesotrophy with the increasing prevalence of dinoflagellates compared to diatoms.

scholarly journals Optimization of Plant Extract Purification Procedure for Rapid Screening Analysis of Sixteen Phenolics by Liquid Chromatography

Separations ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 13
Author(s):  
Petra Ranušová ◽  
Ildikó Matušíková ◽  
Peter Nemeček
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Solid Phase ◽  
Low Cost ◽  
Sinapic Acid ◽  
Rapid Screening ◽  
Coumaric Acid ◽  
Laboratory Equipment ◽  
Wide Range ◽  
Methoxycinnamic Acid

A solid-phase extraction (SPE) procedure was developed for simultaneous monitoring of sixteen different phenolics of various polarity, quantified by high-performance liquid chromatography (HPLC). The procedure allowed screening the accumulation of intermediates in different metabolic pathways that play a crucial role in plant physiology and/or are beneficial for human health. Metabolites mostly involved in phenylpropanoid, shikimate, and polyketide pathways comprise chlorogenic acid, gentisic acid, vanillic acid, caffeic acid, protocatechuic acid, ferulic acid, rutin, quercetin, epicatechin, gallic acid, sinapic acid, p-coumaric acid, o-coumaric acid, vanillin; two rarely quantified metabolites, 2,5-dimethoxybenzoic acid and 4-methoxycinnamic acid, were included as well. The procedure offered low cost, good overall efficiency, and applicability in laboratories with standard laboratory equipment. SPE recoveries were up to 99.8% at various concentration levels. The method allowed for routine analysis of compounds with a wide range of polarity within a single run, while its applicability was demonstrated for various model plant species (tobacco, wheat, and soybean), as well as different tissue types (shoots and roots).

scholarly journals Урожайность различных сортов картофеля и их повреждение вредителями в условиях Удмуртской Республики

2020 ◽  
Author(s):  
O.V. Esenkulova ◽  
О.V. Korobeinikova ◽  
M.P. Maslova
Keyword(s):  
Insect Pests ◽  
Potato Cultivars ◽  
Organic Fertilizers ◽  
Agrotis Segetum ◽  
Potato Tubers ◽  
White Grub ◽  
Severe Damage ◽  
Planting Material ◽  
Udmurt Republic

В рамках развития органического сельского хозяйства актуально выявление сортов с высокой устойчивостью к вредным организмам. В условиях Удмуртской Республики на дерново-среднеподзолистой среднесуглинистой слабосмытой почве в 2017-2018 годах проводились исследования, целью которых была оценка новых и перспективных сортов картофеля различных по срокам созревания. Одной из задач было выявление повреждений клубней вредителями. Изучали сорта картофеля разных сроков созревания: раннеспелые Нандина, Ред Соня, Колетте, Беллароза, Винета, Джоконда, Раноми среднеспелые Джелли, Вираж, Гала, Рябинушка, Каптива, Алуэт позднеспелые Ред Фентази, Церата КВС, Танго. Полевой однофакторный опыт закладывался согласно методике полевого опыта. Клубневой анализ проводили в соответствии с ГОСТ 33996-2016 Картофель семенной. Технические условия и методы определения качества. Коэффициент адаптивности изучаемых сортов картофеля рассчитан по методу Л.А. Животкова. За два года исследований более урожайными были раннеспелые сорта: Нандина, Ред Соня, Беллароза, Раноми среднеспелые: Джелли, Каптива, Алуэт позднеспелые: Ред Фентази и Церата КВС. Повреждение клубней картофеля личинками щелкуна (проволочником) было очень высоким и составило в 2017 году в среднем по сортам 47, в 2018 году 61. В оба года исследований клубни были повреждены личинками озимой совки (в среднем 3-4 ), личинками майского хруща (1-2 ). В 2018 году наблюдалось повреждение полевками (в среднем по сортам 3 ). Раннеспелые сорта являются более адаптивными к условиям Удмуртской Республики (коэффициент адаптивности 1,22 и 1,31). Однако качество посадочного материала не соответствует ГОСТ по проценту повреждений вредителями. Поэтому необходима тщательная сортировка и доведение клубней до регламентируемых показателей. Для снижения поврежденности в полевых условиях картофель требуется размещать после бобовых и крестоцветных культур, проводить известкование почв и вносить перепревшие органические удобрения.As part of the development of organic agriculture, the identification of varieties with high resistance to pests is relevant. In the conditions of the Udmurt Republic, studies were carried out in 2017-2018 on the derno-medium-soil average carbon-free weak soil, the purpose of which was to assess new and permissive potato cultivars of different maturation times. One challenge was to identify damage to tubers by insect pests and the possibility of using them as planting material. Potato cultivars of different maturation dates were studied: early Nandina, Red Sonya, Kolette, Bellaroza, Vineta, Dzhokonda, Ranomi medium Dzhelli, Virazh, Gala, Ryabinushka, Kaptiva, Aluet late Red Fentazi, Tserata KVS, Tango. Field one-factor experiment was laid according to the method of experimental work. Tuberous analysis was carried out in accordance with GOST 33996-2016 Seed Potatoes. Technical conditions and methods of quality determination . The coefficient of adaptivity of the studied potato cultivars is calculated according to the method of L.A. Zhemkov. More crop-producing were early cultivars: Nandina, Red Sonya, Bellaroza, Ranomi average: Dzhelli, Kaptiva, Aluet late: Red Fentazi and Tserata CF. During the years of research, there was damage to potato tubers by wireworm in 2017 on average 47, and in 2018 61. The only cultivar, Ranomi, was not damaged by the wireworm in 2017. A small number of tubers was damaged by larvae of turnip moth (Agrotis segetum) (3-4) and larvae of white grub (1-2). During two years of research in the Udmurt Republic early cultivars were more adaptive, the coefficient of adaptability was 1,31 and 1,22. However, the quality of the planting material by the percentage of damaged tubers by pests does not comply with state standard. For growing in the region, early cultivars Nandin, Red Sonya, Kolette, Bellarosa, Vineta, Dzhokonda, Ranomi are more suitable from middle cultivars Dzhelli, Kaptiva and Aluet turned out to be more stable from late ripening Red Fentazi. Due to severe damage by insect pests, especially wireworms, careful sorting and bringing tubers to regulated parameters, liming of soils, and introduction of rotted organic fertilizers are necessary, as well as to place potatoes in crop rotation after legumes and cruciferous crops.

scholarly journals Effect of soil conditioner (UGmax) application on the content of phenols and glycoalkaloids in potato tubers

2017 ◽  
Vol 63 (No. 5) ◽  
pp. 231-235 ◽  
Author(s):  
Gugała Marek ◽  
Zarzecka Krystyna ◽  
Sikorska Anna ◽  
Kapela Krzysztof ◽  
Niewęgłowski Marek ◽  
...  
Keyword(s):  
Potato Cultivars ◽  
Potato Tubers ◽  
Flower Buds ◽  
Flower Bud ◽  
Soil Conditioner ◽  
Factor I ◽  
Glycoalkaloid Content ◽  
Factor Ii ◽  
Application Methods

The objective of the study was to determine the effect of the soil conditioner UG<sub>max</sub> application on phenol and glycoalkaloid content in the tubers of two table potato cultivars. The following factors were examined: factor I − cultivars: Satina and Tajfun; factor II − five application methods of the soil conditioner UG<sub>max</sub>: (1) control − no UG<sub>max</sub> application; (2) 1.0 L/ha UG<sub>max</sub> before planting; (3) 0.5 L/ha UG<sub>max</sub> before planting + 0.25 L/ha at potato height of 10–15 cm + 0.25 L/ha in flower bud phase; (4) 1.0 L/ha UG<sub>max</sub> before planting + 0.50 L/ha at potato height of 10–15 cm + in 0.50 L/ha in flower bud phase; (5) UG<sub>max</sub> applied after emergence when plants were 10–15 cm high at the rate of 0.5 L/ha, and at the stage of flower buds at the rate of 0.5 L/ha. The highest concentration of phenols and glycoalkaloids was recorded in plots where UG<sub>max</sub> was applied prior to potato planting at the rate of 1.0 L/ha, when plants were 10–15 cm high at the rate of 0.5 L/ha and at the stage of flower buds at the rate of 0.5 L/ha.

scholarly journals The effect of herbicide applications on the content of ascorbic acid and glycoalkaloids in potato tubers

2011 ◽  
Vol 49 (No. 5) ◽  
pp. 237-240 ◽  
Author(s):  
K. Zarzecka ◽  
M. Gugała
Keyword(s):  
Ascorbic Acid ◽  
Statistical Analysis ◽  
Field Experiments ◽  
Potato Cultivars ◽  
Potato Tubers ◽  
Experimental Station

Field experiments were conducted at Zawady Experimental Station (UniversityofPodlasie) in 1999&ndash;2001 to test the effect of herbicides (Sencor 70 WG, Basagran 600 SL) and their mixtures (Sencor 70 WG with Fusilade Super, Basagran 600 SL with Focus Ultra) on the content of ascorbic acid and glycoalkaloids in tubers of three potato cultivars. The application of herbicides to potato fields caused an increase in the content of ascorbic acid in comparison with the control. The statistical analysis showed a&nbsp;significant effect of potato cultivars on ascorbic acid and glycoalkaloid concentrations.

scholarly journals The Impact of Organic vs. Conventional Agricultural Practices on Selected Quality Features of Eight Potato Cultivars

Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 799 ◽  
Author(s):  
Renata Kazimierczak ◽  
Dominika Średnicka-Tober ◽  
Ewelina Hallmann ◽  
Klaudia Kopczyńska ◽  
Krystyna Zarzyńska
Keyword(s):  
Vitamin C ◽  
Agricultural Practices ◽  
Potato Cultivars ◽  
Agricultural System ◽  
Agricultural Systems ◽  
Potato Tubers ◽  
Conventional Production ◽  
The Impact ◽  
Quality Features ◽  
Means Of Production

An organic agricultural system based on natural methods and means of production is an alternative to intensive agriculture. The available research suggests that organic crops, in comparison to the conventional ones, are richer in phenolics and other antioxidants while containing less undesirable pesticide residues and nitrates. The aim of this study was to determine concentrations of polyphenols, lutein, vitamin C, and nitrates in eight potato cultivars (Mazur, Justa, Lawenda, Lech, Tacja, Laskana, Otolia, Magnolia) grown organically and conventionally in a controlled field experiment in Poland. Significant differences between potato tubers of the tested cultivars coming from organic and conventional production were identified for the majority of parameters. Higher concentrations of nitrates and lutein were found in conventional compared to the organic tubers, while organic potatoes were, on average, richer in phenolic compounds. Among the tested cultivars, Magnolia, Otolia, and Laskara were richest in vitamin C and phenolics. Otolia and Laskara also accumulated the highest levels of nitrates. If further confirmed, these observations might be of importance for the producers and consumers, who increasingly search for foods from sustainable and well-controlled agricultural systems.

Medical Technology Transfer in Major Chinese Medical Schools

1991 ◽  
Vol 7 (4) ◽  
pp. 553-560 ◽  
Author(s):  
Teh-wei Hu ◽  
Ying-ying Meng
Keyword(s):  
Technology Transfer ◽  
Economic Reform ◽  
High Performance ◽  
Medical Technology ◽  
Medical Schools ◽  
Laboratory Equipment ◽  
Republic Of China ◽  
Electron Microscopes ◽  
Domestic Products

AbstractThis paper examines how the decision-making process and its consequences affect medical technology transfer in major Chinese medical schools. Data are from a 1987 survey of 13 key medical universities, directly supervised by the Ministry of Public Health in the People's Republic of China. This paper limits itself to four types of laboratory equipment — electron microscopes, UV/VIS spectrophotometers, high-performance liquid chromatographs, and polygraphs. Decisions on the transfer of medical technology have been more decentralized in China since the economic reform in 1978. The major reason for schools to import these four types of equipment is their dissatisfaction with the quality of domestic products. Chinese medical schools depend heavily on the information provided at medical equipment exhibits and their neighboring schools. Their decisions to acquire the equipment are based more on the quality and service available than on the prices. Chinese medical schools face serious infrastructure problems in acquiring and maintaining these pieces of equipment. A number of suggestions are made for improving the efficiency of medical technology transfer in China.

scholarly journals Diazotrophic <i>Trichodesmium</i> impact on UV–Vis radiance and pigment composition in the western tropical South Pacific

2018 ◽  
Vol 15 (16) ◽  
pp. 5249-5269 ◽  
Author(s):  
Cécile Dupouy ◽  
Robert Frouin ◽  
Marc Tedetti ◽  
Morgane Maillard ◽  
Martine Rodier ◽  
...  
Keyword(s):  
High Performance ◽  
New Caledonia ◽  
Chlorophyll Concentration ◽  
Principal Component ◽  
Euphotic Zone ◽  
South Pacific ◽  
Free Fall ◽  
Total Chlorophyll ◽  
Chl A ◽  
Underwater Vision

Abstract. We assessed the influence of the marine diazotrophic cyanobacterium Trichodesmium on the bio-optical properties of western tropical South Pacific (WTSP) waters (18–22∘ S, 160∘ E–160∘ W) during the February–March 2015 OUTPACE cruise. We performed measurements of backscattering and absorption coefficients, irradiance, and radiance in the euphotic zone with a Satlantic MicroPro free-fall profiler and took Underwater Vision Profiler 5 (UPV5) pictures for counting the largest Trichodesmium spp. colonies. Pigment concentrations were determined by fluorimetry and high-performance liquid chromatography and picoplankton abundance by flow cytometry. Trichome concentration was estimated from pigment algorithms and validated by surface visual counts. The abundance of large colonies counted by the UVP5 (maximum 7093 colonies m−3) was well correlated to the trichome concentrations (maximum 2093 trichomes L−1) with an aggregation factor of 600. In the Melanesian archipelago, a maximum of 4715 trichomes L−1 was enumerated in pump samples (3.2 m) at 20∘ S, 167 30∘ E. High Trichodesmium abundance was always associated with absorption peaks of mycosporine-like amino acids (330, 360 nm) and high particulate backscattering, but not with high Chl a fluorescence or blue particulate absorption (440 nm). Along the west-to-east transect, Trichodesmium together with Prochlorococcus represented the major part of total chlorophyll concentration; the contribution of other groups were relatively small or negligible. The Trichodesmium contribution to total chlorophyll concentration was the highest in the Melanesian archipelago around New Caledonia and Vanuatu (60 %), progressively decreased to the vicinity of the islands of Fiji (30 %), and reached a minimum in the South Pacific Gyre where Prochlorococcus dominated chlorophyll concentration. The contribution of Trichodesmium to zeaxanthin was respectively 50, 40 and 20 % for these regions. During the OUTPACE cruise, the relationship between normalized water-leaving radiance (nLw) in the ultraviolet and visible and chlorophyll concentration was similar to that found during the BIOSOPE cruise in the eastern tropical Pacific. Principal component analysis (PCA) of OUTPACE data showed that nLw at 305, 325, 340, 380, 412 and 440 nm was strongly correlated to chlorophyll and zeaxanthin, while nLw at 490 and 565 nm exhibited lower correlations. These results, as well as differences in the PCA of BIOSOPE data, indicated that nLw variability in the greenish blue and yellowish green during OUTPACE was influenced by other variables associated with Trichodesmium presence, such as backscattering coefficient, phycoerythrin fluorescence and/or zeaxanthin absorption, suggesting that Trichodesmium detection should involve examination of nLw in this spectral domain.

scholarly journals Investigation of Streptomyces scabies Causing Potato Scab by Various Detection Techniques, Its Pathogenicity and Determination of Host-Disease Resistance in Potato Germplasm

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 760
Author(s):  
Sohaib Ismail ◽  
Bo Jiang ◽  
Zohreh Nasimi ◽  
M. Inam-ul-Haq ◽  
Naoki Yamamoto ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Common Scab ◽  
Streptomyces Scabies ◽  
Potato Cultivars ◽  
Potato Tubers ◽  
Streptomyces Species ◽  
Detection Techniques ◽  
Streptomyces Spp ◽  
Geographical Regions

Streptomyces scabies is a Gram-positive bacterial pathogen that causes common scab disease to several crops, particularly in the potato. It is a soil borne pathogen, a very devastating scab pathogen and difficult to manage in the field. Streptomyces has several species that cause common scab such as S. scabiei, S. acidiscabies, S. europaeiscabiei, S. luridiscabiei, S. niveiscabiei, S. puniciscabiei, S. reticuliscabiei, S. stelliscabiei, S. turgidiscabies, S. ipomoeae. Common scab disease harmfully affects potato economic and market value due to the presence of black spots on the tuber. Owing to its genetic diversity and pathogenicity, the determination of pathogen presence in potato fields is still challenging. In this study, S. scabies genetic diversity was measured by surveying five potato-growing areas of Pakistan during the growing season 2019. A total of 50 Streptomyces isolates, including S. scabies, S. acidiscabies, S. griseoflavus were isolated and identified based on morphologic, biochemical and molecular analysis. Virulent confirmation assays confirmed ten virulent strains of Streptomyces spp. On the potato cultivars Cardinal and Santee. Among the Streptomyces species, S. scabies showed the highest scab index, followed by S. acidiscabies and S. griseoflavus by exhibiting the scab-like lesions on potato tubers. Ten potato cultivars were screened against these virulent isolates of Streptomyces. The Faisalabad white variety showed the highest scab index followed By Cardinal, Tourag, Kuroda, Santee, Lady Rosetta, Asterix, Diamant, Faisalabad red and Sadaf. Moreover, genetic diversity and pathogenicity of Streptomyces spp. on potato tubers were also likely diverse in different geographical regions and also potato cultivars. This study represents a contribution to understanding the local interaction between potatoes and Streptomyces spp. in Pakistan. It will aid in supporting a solution for the management of this pathogen around the world.

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