scholarly journals Differences in the Effects of Broad-Band UVA and Narrow-Band UVB on Epidermal Keratinocytes

2021 ◽  
Vol 18 (23) ◽  
pp. 12480
Author(s):  
Robert Bajgar ◽  
Anna Moukova ◽  
Nela Chalupnikova ◽  
Hana Kolarova
Keyword(s):  
Cell Viability ◽  
Uv Radiation ◽  
Skin Diseases ◽  
Stratospheric Ozone ◽  
Broad Band ◽  
Epidermal Keratinocytes ◽  
Ros Production ◽  
Uvb Radiation ◽  
Skin Cells ◽  
Radiation Sources

Background: The sun is a natural source of UV radiation. It can be divided into three bands, UVA (315–400 nm), UVB (280–315 nm) and UVC (100–280 nm), where the radiation up to 290 nm is very effectively eliminated by the stratospheric ozone. Although UV radiation can have a beneficial effect on our organism and can be used in the treatment of several skin diseases, it must primarily be considered harmful. Methods: In the presented work, we focused on the study of the longer-wavelength UV components (UVA and UVB) on the human epidermal keratinocyte line HaCaT. As UVA and UVB radiation sources, we used commercially available UVA and UVB tubes from Philips (Philips, Amsterdam, The Netherlands), which are commonly employed in photochemotherapy. We compared their effects on cell viability and proliferation, changes in ROS production, mitochondrial function and the degree of DNA damage. Results: Our results revealed that UVB irradiation, even with significantly lower irradiance, caused greater ROS production, depolarization of mitochondrial membrane potential and greater DNA fragmentation, along with significantly lowering cell viability and proliferative capacity. Conclusions: These results confirm that UV radiation causes severe damages in skin cells, and they need to be protected from it, or it needs to be applied more cautiously, especially if the component used is UVB.

The effects of ultraviolet-B radiation on freshwater ecosystems of the Arctic: Influence from stratospheric ozone depletion and climate change

2004 ◽  
Vol 12 (1) ◽  
pp. 1-70 ◽  
Author(s):  
S Perin ◽  
D RS Lean
Keyword(s):  
Climate Change ◽  
Uv Radiation ◽  
Climate Warming ◽  
Ozone Depletion ◽  
Aquatic Ecosystems ◽  
Stratospheric Ozone ◽  
Aquatic Organisms ◽  
Ultraviolet B ◽  
The Arctic ◽  
Uvb Radiation

Depletion of stratospheric ozone, the principal atmospheric attenuator of ultraviolet-B (UVB) radiation, by man-made chemicals has raised scientific and public concern regarding the biological effects of increased UVB radiation on Earth. There is an increased awareness that existing levels of solar UV radiation have an important influence on biological and chemical processes in aquatic ecosystems. For aquatic organisms, numerous studies have shown direct detrimental effects of UVB radiation at each trophic level. Fortunately, many aquatic organisms also possess a range of photoprotective mechanisms against UV radiation toxicity. In addition to its direct impact, harmful effects of UVB radiation at a single-trophic level can cascade through the food web and indirectly affect organisms from other trophic levels. Because UV radiation photochemically reacts with humic substances and other photosensitive agents in the water, increases in solar UVB can also indirectly affect aquatic organisms through the production and (or) release of different photoproducts like biologically available nutrients and harmful reactive oxygen species. Polar aquatic ecosystems have been of particular concern, since stratospheric ozone-related UVB increases have been the greatest in these regions. With the influences of climate warming and the possibility of future volcanic eruptions, ozone losses are expected to get worse in the Arctic stratosphere, and the ozone layer recovery may not follow the slow decline of industrial ozone-depleting compounds in the atmosphere. Climate warming is also expected to bring important changes in underwater ultraviolet radiation (UVR) penetration in Arctic freshwaters that would be more significant to the aquatic biota than stratospheric ozone depletion.Key words: Arctic, UV radiation, UVB, ozone depletion, climate change, aquatic ecosystems.

scholarly journals Antioxidant and Anti-inflammatory Effect of Cannabidiol Contributes to the Decreased Lipid Peroxidation of Keratinocytes of Rat Skin Exposed to UV Radiation

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Anna Jastrząb ◽  
Iwona Jarocka-Karpowicz ◽  
Agnieszka Markowska ◽  
Adam Wroński ◽  
Agnieszka Gęgotek ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Uv Radiation ◽  
Physical Factor ◽  
Uvb Radiation ◽  
Rat Skin ◽  
Metabolic Disturbances ◽  
Skin Cells ◽  
Anti Inflammatory ◽  
Proinflammatory Factors

There is a great need for compounds with antioxidant and anti-inflammatory properties for protection against UV radiation, which is the most prooxidative physical factor that skin cells are exposed to everyday. Therefore, the aim of the study was to evaluate the mechanism of phytocannabinoid-cannabidiol (CBD) action in vivo on lipid metabolism in keratinocytes of rat skin exposed to UVA/UVB radiation. Our results show that CBD protects keratinocytes against the effects of UVA/UVB radiation by reducing lipid peroxidation products: 4-HNE and 8-isoPGF2α. In addition, CBD significantly increases the level of endocannabinoids, such as anandamide, 2-arachidonylglycerol, and palmitoylethanolamide, and the activation of their receptors CB1/2 or TRPV1. The above changes are due to the protective effect of CBD against the UVA/UVB-induced decrease in the level/activity of superoxide dismutase and the components of the thioredoxin and glutathione systems. CBD also increases the in vivo transcriptional activity of Nrf2 and the expression of its Bach1 inhibitor as well as preventing the UVA/UVB-induced increase in the expression of Nrf2 activators p21, p62, p38, and KAP1 and proinflammatory factors such as NFκB and TNFα. By counteracting oxidative stress and changes in lipid structure in keratinocytes, CBD prevents cellular metabolic disturbances, protecting the epidermis against UV damage.

scholarly journals Development of a high-throughput screening system for identification of novel reagents regulating DNA damage in human dermal fibroblasts

2015 ◽  
Vol 65 (3) ◽  
pp. 331-341 ◽  
Author(s):  
Seunghee Bae ◽  
In-Sook An ◽  
Sungkwan An
Keyword(s):  
Dna Damage ◽  
Uv Radiation ◽  
High Throughput ◽  
High Throughput Screening ◽  
Dermal Fibroblasts ◽  
Hypoxanthine Phosphoribosyl Transferase ◽  
Screening System ◽  
Uvb Radiation ◽  
Skin Cells ◽  
F Cells

Abstract Ultraviolet (UV) radiation is a major inducer of skin aging and accumulated exposure to UV radiation increases DNA damage in skin cells, including dermal fibroblasts. In the present study, we developed a novel DNA repair regulating material discovery (DREAM) system for the high-throughput screening and identification of putative materials regulating DNA repair in skin cells. First, we established a modified lentivirus expressing the luciferase and hypoxanthine phosphoribosyl transferase (HPRT) genes. Then, human dermal fibroblast WS-1 cells were infected with the modified lentivirus and selected with puromycin to establish cells that stably expressed luciferase and HPRT (DREAM-F cells). The first step in the DREAM protocol was a 96-well-based screening procedure, involving the analysis of cell viability and luciferase activity after pretreatment of DREAM-F cells with reagents of interest and post-treatment with UVB radiation, and vice versa. In the second step, we validated certain effective reagents identified in the first step by analyzing the cell cycle, evaluating cell death, and performing HPRT-DNA sequencing in DREAM-F cells treated with these reagents and UVB. This DREAM system is scalable and forms a time-saving high-throughput screening system for identifying novel anti-photoaging reagents regulating DNA damage in dermal fibroblasts.

The potential cutaneous effects of stratospheric ozone depletion

1996 ◽  
Vol 4 (1) ◽  
pp. 1-7
Author(s):  
John H. Epstein
Keyword(s):  
Skin Cancer ◽  
Lupus Erythematosus ◽  
Skin Diseases ◽  
Stratospheric Ozone ◽  
Ultraviolet B ◽  
Uvb Radiation ◽  
Skin Cancers ◽  
Light Skin ◽  
Solar Urticaria ◽  
Common Skin

Recent evidence indicates that there has been a reduction in the stratospheric ozone over the northern hemisphere, as well as the Antarctic and Arctic latitudes. This has resulted in an increased penetration of ultraviolet B (UVB) at least as measured at Toronto, Canada, since 1989. If no precautions are observed by the human population, this could eventually result in an increase in the skin cancer incidence. This would be especially true for the most common cancers, that is, the nonmelanoma skin cancers (NMSCs), basal cell carcinomas and squamous cell carcinomas. In addition it has been predicted that the third most common skin cancer, the malignant melanoma, would also increase in incidence. However, the relationship between UVB radiation and melanoma formation is much less clear than it is for NMSCs. Clinically people with a loss or lack of melanin protection such as those with occulocutaneous albinism and vitiligo, or much more commonly, people with light skin, eyes, and hair would be at greatest risk. Also increased UVB penetration could exacerbate certain infections such as herpes simplex. People with UVB-sensitive diseases including solar urticaria, polymorphous light eruptions, lupus erythematosus, dermatomyositis, pemphigus, pemphigoid, Darier's disease, familial benign chronic pemphigus, and certain recessive degenerative genodermatoses would also be potentially more vulnerable.Key words: ozone, ultraviolet B (UVB), skin cancer, photosensitive skin diseases.

scholarly journals The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes

2021 ◽  
Vol 22 (3) ◽  
pp. 1501
Author(s):  
Agnieszka Gęgotek ◽  
Sinemyiz Atalay ◽  
Adelina Rogowska-Wrzesińska ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Uv Radiation ◽  
Intracellular Signaling ◽  
De Novo ◽  
Protein Biosynthesis ◽  
Protein Structures ◽  
Epidermal Keratinocytes ◽  
Environmental Damage ◽  
Human Epidermal Keratinocytes ◽  
Skin Cells ◽  
Induced Changes

Human epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA/B irradiated keratinocytes. The keratinocytes were cultured in a three-dimensional (3D) system, designed to mimic epidermal conditions closely. The obtained results indicate that CBD protected against the harmful effects of UVA/B radiation. CBD decreased the expression of proinflammatory proteins, including TNFα/NFκB and IκBKB complex and decreased the expression of proteins involved in de novo protein biosynthesis, which are increased in UVA/B-irradiated cells. Additionally, CBD enhanced the UV-induced expression of 20S proteasome subunits. CBD also protected protein structures from 4-hydroxynonenal (HNE)-binding induced by UV radiation, which primarily affects antioxidant enzymes. CBD-through its antioxidant/anti-inflammatory activity and regulation of protein biosynthesis and degradation-protects skin cells against UVA/B-induced changes. In the future, its long-term use in epidermal cells should be investigated.

scholarly journals Resveratrol-Enriched Rice Attenuates UVB-ROS-Induced Skin Aging via Downregulation of Inflammatory Cascades

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Lalita Subedi ◽  
Taek Hwan Lee ◽  
Hussain Mustatab Wahedi ◽  
So-Hyeon Baek ◽  
Sun Yeou Kim
Keyword(s):  
Cytochrome C ◽  
Adverse Effects ◽  
Matrix Metalloproteinase ◽  
Uv Radiation ◽  
Chronic Exposure ◽  
Caspase 3 ◽  
Skin Aging ◽  
Ros Production ◽  
Uvb Radiation ◽  
Cox 2

The skin is the outermost protective barrier between the internal and external environments in humans. Chronic exposure to ultraviolet (UV) radiation is a major cause of skin aging. UVB radiation penetrates the skin and induces ROS production that activates three major skin aging cascades: matrix metalloproteinase- (MMP-) 1-mediated aging; MAPK-AP-1/NF-κB-TNF-α/IL-6, iNOS, and COX-2-mediated inflammation-induced aging; and p53-Bax-cleaved caspase-3-cytochrome C-mediated apoptosis-induced aging. These mechanisms are collectively responsible for the wrinkling and photoaging characteristic of UVB-induced skin aging. There is an urgent requirement for a treatment that not only controls these pathways to prevent skin aging but also avoids the adverse effects often encountered when applying bioactive compounds in concentrated doses. In this study, we investigated the efficacy of genetically modified normal edible rice (NR) that produces the antiaging compound resveratrol (R) as a treatment for skin aging. This resveratrol-enriched rice (RR) overcomes the drawbacks of R and enhances its antiaging potential by controlling the abovementioned three major pathways of skin aging. RR does not exhibit the toxicity of R alone and promisingly downregulates the pathways underlying UVB-ROS-induced skin aging. These findings advocate the use of RR as a nutraceutical for antiaging purposes.

Neutrophil Myeloperoxidase Destruction by Ultraviolet Irradiation

1988 ◽  
Vol 46 ◽  
pp. 138-139
Author(s):  
J. Hanker ◽  
B. Giammara ◽  
G. Strauss
Keyword(s):  
Uv Radiation ◽  
Myeloid Leukemia ◽  
Stratospheric Ozone ◽  
Psoriasis Patient ◽  
Whole Body ◽  
Human Neutrophils ◽  
Cupric Nitrate ◽  
The Earth ◽  
Routine Handling ◽  
Uvb Phototherapy

Only a fraction of the UV radiation emitted by the sun reaches the earth; most of the UVB (290-320nm) is eliminated by stratospheric ozone. There is increasing concern, however, that man-made chemicals are damaging this ozone layer. Although the effects of UV on DNA or as a carcinogen are widely known, preleukemia and acute myeloid leukemia (AML) have only rarely been reported in psoriasis patients treated with 8-methoxypsoralen and UV (PUVA). It was therefore of interest to study the effects of UV on the myeloperoxidase (MP) activity of human neutrophils. The peroxidase activity of enriched leukocyte preparations on coverslips was shown cytochemically with a diaminobenzidine medium and cupric nitrate intensification.Control samples (Figs. 1,4,5) of human bloods that were not specifically exposed to UV radiation or light except during routine handling were compared with samples which had been exposed in one of several different ways. One preparation (Fig. 2) was from a psoriasis patient who had received whole-body UVB phototherapy repeatedly.

scholarly journals Cosmetic and Dermatological Properties of Selected Ayurvedic Plant Extracts

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 614
Author(s):  
Martyna Zagórska-Dziok ◽  
Aleksandra Ziemlewska ◽  
Tomasz Bujak ◽  
Zofia Nizioł-Łukaszewska ◽  
Zofia Hordyjewicz-Baran
Keyword(s):  
Skin Diseases ◽  
Multiple Reaction Monitoring ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Cell Protection ◽  
Skin Cells ◽  
Skin Cell ◽  
Aging Properties ◽  
Enzyme Superoxide Dismutase

Due to the constantly growing interest in ingredients of natural origin, this study attempts to evaluate the possibility of using extracts from three Ayurvedic plants in preparations for the care and treatment of skin diseases. Therefore, studies of antioxidant properties were carried out using DPPH and ABTS radicals, obtaining 76% and 88% of these radical scavenging, respectively. A significant decrease in the intracellular level of free radicals and an increase in the activity of the antioxidant enzyme-superoxide dismutase by almost 60% were also observed. In addition, the extracts were assessed for anti-inflammatory and anti-aging properties, obtaining over 70% inhibition of lipoxygenase activity and almost 40% of collagenase. Additionally, the cytoprotective properties of the obtained extracts on skin cells, keratinocytes and fibroblasts, were demonstrated. To assess the content of biologically active compounds, HPLC-electrospray ionization (ESI)-MS/MS multiple reaction monitoring (MRM) analyses were performed. The obtained results show that all three analyzed plants are a valuable source of biologically active substances with desired properties in the context of skin cell protection. Particularly noteworthy is the extract of Epilobium angustifolium L., for which the most promising results were obtained.

scholarly journals Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1260
Author(s):  
Agnieszka Gęgotek ◽  
Anna Jastrząb ◽  
Marta Dobrzyńska ◽  
Michał Biernacki ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Cell Viability ◽  
Human Skin ◽  
Phospholipid Fatty Acid ◽  
Antioxidant Properties ◽  
Endocannabinoid System ◽  
Membrane Phospholipids ◽  
Skin Cells ◽  
Human Skin Cells ◽  
Induced Changes

Natural antioxidants effectively counteract changes caused by UV radiation in human skin cells. However, their action is limited due to their lipo/hydrophilicity. Therefore, the aim of this study was to analyze the mutual protective action of hydrophilic ascorbic acid and partially lipophilic rutin against UVA/UVB-induced changes in membranes phospholipid and endocannabinoid system in keratinocytes and fibroblasts. Obtained results clearly showed that, despite the stronger antioxidant properties of ascorbic acid, the lipid membranes were more effectively protected against UV-induced oxidation by rutin, including changes in phospholipid fatty acid levels, prevention against reactive aldehydes formation and endocannabinoids degradation. Ascorbic acid more strongly prevented UV-induced endocannabinoid receptors expression in fibroblasts, especially CB1. However, the combined action of used antioxidants resulted in the greatest cytoprotective effect, which was evident in the inflammatory marker TNFα down-regulation and increased cell viability following cell irradiation. The applied mixture of antioxidants showed a stronger protective in relation to membrane phospholipids in keratinocytes and in the endocannabinoid system in fibroblasts. In conclusion, it can be suggested that combined antioxidant capacities of ascorbic acid and rutin protects against lipid peroxidation but also decreases the UV-induced inflammation by direct interaction with the endocannabinoid system, thus increasing skin cell viability.

scholarly journals Molecular and Biochemical Basis of Minocycline-Induced Hyperpigmentation—The Study on Normal Human Melanocytes Exposed to UVA and UVB Radiation

2021 ◽  
Vol 22 (7) ◽  
pp. 3755
Author(s):  
Jakub Rok ◽  
Zuzanna Rzepka ◽  
Justyna Kowalska ◽  
Klaudia Banach ◽  
Artur Beberok ◽  
...  
Keyword(s):  
Uv Radiation ◽  
Therapeutic Potential ◽  
Melanin Synthesis ◽  
Uvb Radiation ◽  
Biochemical Basis ◽  
Skin Hyperpigmentation ◽  
Human Melanocytes ◽  
Anti Cancer ◽  
Normal Human

Minocycline is a drug which induces skin hyperpigmentation. Its frequency reaches up to 50% of treated patients. The adverse effect diminishes the great therapeutic potential of minocycline, including antibacterial, neuroprotective, anti-inflammatory and anti-cancer actions. It is supposed that an elevated melanin level and drug accumulation in melanin-containing cells are related to skin hyperpigmentation. This study aimed to evaluate molecular and biochemical mechanism of minocycline-induced hyperpigmentation in human normal melanocytes, as well as the contribution of UV radiation to this side effect. The experiments involved the evaluation of cyto- and phototoxic potential of the drug using cell imaging with light and confocal microscopes as well as biochemical and molecular analysis of melanogenesis. We showed that minocycline induced melanin synthesis in epidermal melanocytes. The action was intensified by UV irradiation, especially with the UVB spectrum. Minocycline stimulated the expression of microphthalmia-associated transcription factor (MITF) and tyrosinase (TYR) gene. Higher levels of melanin and increased activity of tyrosinase were also observed in treated cells. Moreover, minocycline triggered the supranuclear accumulation of tyrosinase, similar to UV radiation. The decreased level of premelanosome protein PMEL17 observed in all minocycline-treated cultures suggests disorder of the formation, maturation or distribution of melanosomes. The study revealed that minocycline itself was able to enhance melanin synthesis. The action was intensified by irradiation, especially with the UVB spectrum. Demonstrated results confirmed the potential role of melanin and UV radiation minocycline-induced skin hyperpigmentation.

Sign in / Sign up

Export Citation Format

Share Document