Proteomic Analysis Reveals Coordinated Regulation of Anthocyanin Biosynthesis through Signal Transduction and Sugar Metabolism in Black Rice Leaf

Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways.

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Proteomic Analysis of the Function of a Novel Cold-Regulated Multispanning Transmembrane Protein COR413-PM1 in Arabidopsis

International Journal of Molecular Sciences ◽

10.3390/ijms19092572 ◽

2018 ◽

Vol 19 (9) ◽

pp. 2572 ◽

Cited By ~ 5

Author(s):

Chen Su ◽

Kai Chen ◽

Qingqian Ding ◽

Yongying Mou ◽

Rui Yang ◽

...

Keyword(s):

Fatty Acids ◽

Plasma Membrane ◽

Fatty Acid ◽

Proteomic Analysis ◽

Fatty Acid Biosynthesis ◽

Transmembrane Protein ◽

Sugar Metabolism ◽

Freezing Stress ◽

Plant Tolerance ◽

Phosphoribosyl Pyrophosphate

The plasma membrane is the first subcellular organ that senses low temperature, and it includes some spanning transmembrane proteins that play important roles in cold regulation. COR413-PM1 is a novel multispanning transmembrane cold-regulated protein; however, the related functions are not clear in Arabidopsis. We found the tolerance to freezing stress of cor413-pm1 was lower than wild-type (WT). A proteomics method was used to analyze the differentially abundant proteins (DAPs) between cor413-pm1 and WT. A total of 4143 protein groups were identified and 3139 were accurately quantitated. The DAPs associated with COR413-PM1 and freezing treatment were mainly involved in the metabolism of fatty acids, sugars, and purine. Quantitative real-time PCR (qRT-PCR) confirmed the proteomic analysis results of four proteins: fatty acid biosynthesis 1 (FAB1) is involved in fatty acid metabolism and might affect the plasma membrane structure; fructokinase 3 (FRK3) and sucrose phosphate synthase A1 (SPSA1) play roles in sugar metabolism and may influence the ability of osmotic adjustment under freezing stress; and GLN phosphoribosyl pyrophosphate amidotransferase 2 (ASE2) affects freezing tolerance through purine metabolism pathways. In short, our results demonstrate that the multispanning transmembrane protein COR413-PM1 regulates plant tolerance to freezing stress by affecting the metabolism of fatty acids, sugars, and purine in Arabidopsis.

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Coordinated regulation of photosynthesis and sugar metabolism in guar increases tolerance to drought

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2021.104701 ◽

2021 ◽

pp. 104701

Author(s):

Komal Pandey ◽

Ravi Shankar Kumar ◽

Priti Prasad ◽

Veena Pande ◽

Prabodh Kumar Trivedi ◽

...

Keyword(s):

Sugar Metabolism ◽

Coordinated Regulation ◽

Regulation Of Photosynthesis

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Genome-Wide Gene Expression Profiles Analysis Reveal Novel Insights into Drought Stress in Foxtail Millet (Setaria italica L.)

International Journal of Molecular Sciences ◽

10.3390/ijms21228520 ◽

2020 ◽

Vol 21 (22) ◽

pp. 8520

Author(s):

Ling Qin ◽

Erying Chen ◽

Feifei Li ◽

Xiao Yu ◽

Zhenyu Liu ◽

...

Keyword(s):

Gene Expression ◽

Signal Transduction ◽

Drought Stress ◽

Expression Profiles ◽

Expression Patterns ◽

Foxtail Millet ◽

Gene Expression Profiles ◽

Sugar Metabolism ◽

Setaria Italica ◽

Phenylpropanoid Biosynthesis

Foxtail millet (Setaria italica (L.) P. Beauv) is an important food and forage crop because of its health benefits and adaptation to drought stress; however, reports of transcriptomic analysis of genes responding to re-watering after drought stress in foxtail millet are rare. The present study evaluated physiological parameters, such as proline content, p5cs enzyme activity, anti-oxidation enzyme activities, and investigated gene expression patterns using RNA sequencing of the drought-tolerant foxtail millet variety (Jigu 16) treated with drought stress and rehydration. The results indicated that drought stress-responsive genes were related to many multiple metabolic processes, such as photosynthesis, signal transduction, phenylpropanoid biosynthesis, starch and sucrose metabolism, and osmotic adjustment. Furthermore, the Δ1-pyrroline-5-carboxylate synthetase genes, SiP5CS1 and SiP5CS2, were remarkably upregulated in foxtail millet under drought stress conditions. Foxtail millet can also recover well on rehydration after drought stress through gene regulation. Our data demonstrate that recovery on rehydration primarily involves proline metabolism, sugar metabolism, hormone signal transduction, water transport, and detoxification, plus reversal of the expression direction of most drought-responsive genes. Our results provided a detailed description of the comparative transcriptome response of foxtail millet variety Jigu 16 under drought and rehydration environments. Furthermore, we identify SiP5CS2 as an important gene likely involved in the drought tolerance of foxtail millet.

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Transcriptome Response to Drought, Rehydration and Re-Dehydration in Potato

International Journal of Molecular Sciences ◽

10.3390/ijms21010159 ◽

2019 ◽

Vol 21 (1) ◽

pp. 159 ◽

Cited By ~ 2

Author(s):

Yongkun Chen ◽

Canhui Li ◽

Jing Yi ◽

Yu Yang ◽

Chunxia Lei ◽

...

Keyword(s):

Signal Transduction ◽

Gene Regulation ◽

Lipid Metabolism ◽

Drought Stress ◽

Sugar Metabolism ◽

Rna Seq ◽

Drought Tolerant ◽

Cell Components ◽

Drought Hardening

Potato is an important food crop and its production is susceptible to drought. Drought stress in crop growth is usually multiple- or long-term. In this study, the drought tolerant potato landrace Jancko Sisu Yari was treated with drought stress, rehydration and re-dehydration, and RNA-seq was applied to analyze the characteristics of gene regulation during these treatments. The results showed that drought-responsive genes mainly involved photosynthesis, signal transduction, lipid metabolism, sugar metabolism, wax synthesis, cell wall regulation, osmotic adjustment. Potato also can be recovered well in the re-emergence of water through gene regulation. The recovery of rehydration mainly related to patatin, lipid metabolism, sugar metabolism, flavonoids metabolism and detoxification besides the reverse expression of the most of drought-responsive genes. The previous drought stress can produce a positive responsive ability to the subsequent drought by drought hardening. Drought hardening was not only reflected in the drought-responsive genes related to the modified structure and cell components, but also in the hardening of gene expression or the “memory” of drought-responsive genes. Abundant genes involved photosynthesis, signal transduction, sugar metabolism, protease and protease inhibitors, flavonoids metabolism, transporters and transcription factors were subject to drought hardening or memorized drought in potato.

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Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)

International Journal of Molecular Sciences ◽

10.3390/ijms20040962 ◽

2019 ◽

Vol 20 (4) ◽

pp. 962 ◽

Cited By ~ 9

Author(s):

Jianxin Bian ◽

Pingchuan Deng ◽

Haoshuang Zhan ◽

Xiaotong Wu ◽

Mutthanthirige Nishantha ◽

...

Keyword(s):

Signal Transduction ◽

Rna Editing ◽

Signal Transduction Pathway ◽

Sugar Metabolism ◽

Transcriptome Profiling ◽

Barley Grain ◽

Grain Development ◽

Hordeum Vulgare L ◽

Functional Studies ◽

Transcriptional Dynamics

Grain development, as a vital process in the crop’s life cycle, is crucial for determining crop quality and yield. However, the molecular basis and regulatory network of barley grain development is not well understood at present. Here, we investigated the transcriptional dynamics of barley grain development through RNA sequencing at four developmental phases, including early prestorage phase (3 days post anthesis (DPA)), late prestorage or transition phase (8 DPA), early storage phase (13 DPA), and levels off stages (18 DPA). Transcriptome profiling found that pronounced shifts occurred in the abundance of transcripts involved in both primary and secondary metabolism during grain development. The transcripts’ activity was decreased during maturation while the largest divergence was observed between the transitions from prestorage phase to storage phase, which coincided with the physiological changes. Furthermore, the transcription factors, hormone signal transduction-related as well as sugar-metabolism-related genes, were found to play a crucial role in barley grain development. Finally, 4771 RNA editing events were identified in these four development stages, and most of the RNA editing genes were preferentially expressed at the prestore stage rather than in the store stage, which was significantly enriched in “essential” genes and plant hormone signal transduction pathway. These results suggested that RNA editing might act as a ‘regulator’ to control grain development. This study systematically dissected the gene expression atlas of barley grain development through transcriptome analysis, which not only provided the potential targets for further functional studies, but also provided insights into the dynamics of gene regulation underlying grain development in barley and beyond.

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