scholarly journals Hydrophobicity-Tuned Periodic Mesoporous Organo-Silica Nanoparticles for Photodynamic Therapy

2020 ◽  
Vol 21 (7) ◽  
pp. 2586
Author(s):  
Chia-Hui Lin ◽  
Ranjith Kumar Kankala ◽  
Prabhakar Busa ◽  
Chia-Hung Lee
Keyword(s):  
Photodynamic Therapy ◽  
Physicochemical Properties ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Loading ◽  
Membrane Damage ◽  
Protoporphyrin Ix ◽  
Hybrid Nanocomposites ◽  
Cell Membrane Damage

Since their invention, periodic mesoporous organosilicas (PMOs), an innovative class of materials based on organic as well as inorganic hybrid nanocomposites, have gathered enormous interest owing to their advantageous physicochemical attributes over the pristine mesoporous silica nanoparticles (MSNs). To further increase the interactions with the therapeutic guest species and subsequent compatibility as well as the physicochemical properties of PMOs, we demonstrate the post-hydroxylation of benzene-bridged PMO-based nanoparticles for photodynamic therapy (PDT). Initially, the hydrophobic benzene group in the PMO framework is modified through electrophilic substitution-assisted hydroxylation mediated by Fenton as well as Fenton-like reactions utilizing divalent and trivalent metal salts, respectively. These post-grafted PMOs with tuned hydrophobicity resulted in improved biocompatibility as well as drug loading efficiency through governing the interactions in host–guest chemistry by changing the physicochemical properties of the PMO frameworks. Furthermore, the photosensitizer, protoporphyrin IX (PpIX) molecules, encapsulated in the PMO frameworks showed a significant PDT effect in colon carcinoma (HT-29 cell line) and Gram-negative bacterial strain, Escherichia coli (E. coli). Furthermore, the light-induced cytotoxic properties in vitro are confirmed by various tests, including lactate dehydrogenase (LDH) assay for cell membrane damage and caspase assay for apoptosis determination. Indeed, the delivered PpIX molecules from PMOs generated deadly singlet oxygen species intracellularly under visible light irradiation, resulting in cell death through concomitantly triggered apoptotic caspases. Together, our findings demonstrate that this post-modified PMO design is highly advantageous and can be used as an effective PDT platform.

scholarly journals Polymeric Mesoporous Silica Nanoparticles for Enhanced Delivery of 5-Fluorouracil In Vitro

Pharmaceutics ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 288 ◽  
Author(s):  
Thashini Moodley ◽  
Moganavelli Singh
Keyword(s):  
Side Effects ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Therapeutic Potential ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Loading ◽  
Hek293 Cells ◽  
Cancer Drug ◽  
Delivery Vehicles

There is a need for the improvement of conventional cancer treatment strategies by incorporation of targeted and non-invasive procedures aimed to reduce side-effects, drug resistance, and recurrent metastases. The anti-cancer drug, 5-fluorouracil (5-FU), is linked to a variety of induced-systemic toxicities due to its lack of specificity and potent administration regimens, necessitating the development of delivery vehicles that can enhance its therapeutic potential, while minimizing associated side-effects. Polymeric mesoporous silica nanoparticles (MSNs) have gained popularity as delivery vehicles due to their high loading capacities, biocompatibility, and good pharmacokinetics. MSNs produced in this study were functionalized with the biocompatible polymers, chitosan, and poly(ethylene)glycol to produce monodisperse NPs of 36–65 nm, with a large surface area of 710.36 m2/g, large pore volume, diameter spanning 9.8 nm, and a favorable zeta potential allowing for stability and enhanced uptake of 5-FU. Significant drug loading (0.15–0.18 mg5FU/mgmsn), controlled release profiles (15–65%) over 72 hours, and cell specific cytotoxicity in cancer cells (Caco-2, MCF-7, and HeLa) with reduced cell viability (≥50%) over the non-cancer (HEK293) cells were established. Overall, these 5FU-MSN formulations have been shown to be safe and effective delivery systems in vitro, with potential for in vivo applications.

scholarly journals 20-nm-sized mesoporous silica nanoparticles with porphyrin photosensitizers for in vitro photodynamic therapy

2016 ◽  
Vol 79 (3) ◽  
pp. 447-456 ◽  
Author(s):  
Chiara Mauriello Jimenez ◽  
Yolanda Galàn Rubio ◽  
Valentin Saunier ◽  
David Warther ◽  
Vanja Stojanovic ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Porphyrin Photosensitizers ◽  
20 Nm

scholarly journals In vitro Studies of Functionalized Mesoporous Silica Nanoparticles for Photodynamic Therapy

2009 ◽  
Vol 21 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Hsiung-Lin Tu ◽  
Yu-Shen Lin ◽  
Hsia-Yu Lin ◽  
Yann Hung ◽  
Leu-Wei Lo ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
In Vitro Studies ◽  
Functionalized Mesoporous Silica

scholarly journals Functionalized Mesoporous Silica Nanoparticles as Delivery Systems for Doxorubicin: Drug Loading and Release

2021 ◽  
Vol 11 (13) ◽  
pp. 6121
Author(s):  
Candace M. Day ◽  
Martin J. Sweetman ◽  
Yunmei Song ◽  
Sally E. Plush ◽  
Sanjay Garg
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Release Kinetics ◽  
Drug Loading ◽  
In Vitro Release ◽  
Delivery Systems ◽  
Stimuli Responsive ◽  
Kinetics Of

Functionalized nanoparticles have played a major role in the field of targeted therapy, owing to their ability to control the release and for the selective delivery of entrapped materials to tumours. In this work, we described the loading capacity and in vitro release kinetics of mesoporous silica nanoparticles (MSNs), functionalized with Poly-L-Histidine and Tamoxifen. The model drug Doxorubicin (DOX) was successfully encapsulated into MSN-based systems, using the technique of solvent immersion. A post-surface grafting loading method was investigated on functionalized systems, with DOX loading content determined using HPLC. Dialysis bag diffusion was employed to investigate the release kinetics of DOX-loaded-systems at pH 7.4 and 5. The amount of DOX released from native MSNs systems over a 72 h period at pH 5 was approximately 40%; and at pH 7.4 ≈ 30%. A moderate pH dependent release behaviour was observed with both our functionalized systems: DOX@MSN-PLH and DOX@MSN-PLH-TAM; with approximately 5% of DOX released from DOX@MSN-PLH-TAM at pH 7.4 and about 9% released at pH 7.4 over 72 h. The maximal cumulated release of DOX molecules from DOX@MSN-PLH after 72 h was ≈ 18% at pH 7.4 and ≈ 23% at pH 5, respectively. The outcome of this work offers a promising contribution towards building future stimuli-responsive nano-drug delivery systems.

Erythrocyte-cancer hybrid membrane-camouflaged mesoporous silica nanoparticles loaded with Gboxin for glioma-targeting therapy

2021 ◽  
Vol 22 ◽  
Author(s):  
Xiuxiu Jiao ◽  
Xiaoyan Yu ◽  
Chunai Gong ◽  
Hao Zhu ◽  
Bin Zhang ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Delivery System ◽  
Cell Model ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Loading ◽  
Circulation System ◽  
Normal Cells ◽  
Self Recognition

Objective: The purpose of this research is to formulate a biomimetic drug delivery system that can selectively target glioblastoma (GBM) to deliver the antitumor agent, Gboxin: a novel Complex V inhibitor. Gboxin can specifically inhibit GBM cell growth but not normal cells. Methods: In the present study, we utilized Red Blood Cell (RBC) membrane and U251 cell membrane to obtain a hybrid biomimetic membrane (RBC-U), and prepared RBC-U coated Gboxin-loaded mesoporous silica nanoparticles ((MSNs/Gboxin)@[RBC-U]) for GBM chemotherapy. The zeta potential, particle size, and morphology of (MSNs/Gboxin)@[RBC-U] were characterized. The cellular uptake, effect of cells growth inhibition, biocompatibility, and specific self-recognition of nanoparticles were evaluated. Results: The (MSNs/Gboxin)@[RBC-U] was successfully fabricated and possessed high stability in the circulation system. The drug loading of Gboxin was 13.9%. (MSNs/Gboxin)@[RBC-U], effectively retain drugs in the physiological environment and releasing Gboxin rapidly in the tumor cells. Compared to the MSNs/Gboxin, the (MSNs/Gboxin)@[RBC-U] exhibited highly specific self-recognition to the source cell line. Additionally, the (MSNs/Gboxin)@[RBC-U] showed excellent anti-proliferation efficiency (IC50 = 0.21 μg/mL) in the tumor cell model and a few side effects in normal cells in vitro. Conclusion: The (MSNs/Gboxin)@[RBC-U] exhibited significant anti-cancer effects in vitro and the specific self-recognition to GBM cells. Hence, (MSNs/Gboxin)@[RBC-U] could be a promising delivery system for GBM targeted therapy.

Preparation of Mesoporous Silica Nanoparticles for Insulin Drug Delivery

2013 ◽  
Vol 829 ◽  
pp. 251-257 ◽  
Author(s):  
Abdollah Zakeri Siavashani ◽  
Masoume Haghbin Nazarpak ◽  
Fateme Fayyaz Bakhsh ◽  
Tayebeh Toliyat ◽  
Mehran Solati-Hashjin
Keyword(s):  
Drug Delivery ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Size Distribution ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Carrier ◽  
Drug Loading ◽  
Hydrothermal Process ◽  
Analytical Techniques

This study has focused on mesoporous silica nanoparticles as a drug delivery system of insulin, which was synthesized via a hydrothermal process. The morphology and composition of the silica nanoparticles were characterized by different analytical techniques such as Scanning Electron Microscope (SEM), X-Ray Diffraction Analysis (XRD), Fourier Transform Infrared spectroscopy (FTIR) and BrunauerEmmettTeller (BET). The percentage of drug loading and the in vitro drug release properties of the mesoporous silica nanoparticles in gastrointestinal tract were investigated in simulated gastrointestinal conditions by ultraviolet-visible spectroscopy. The results showed the amorphous structure of SBA-15 in mesoporous silica particles has a narrow pore size distribution. Also, particles shape was nearly wheat-like with almost homogeneous size distribution. Furthermore, it was revealed that the mesoporous silica nanoparticles have a high insulin loading and release capacity. These prominent behaviors make mesoporous silica nanoparticles promising material as a drug carrier for insulin delivery.

Stimuli-responsive protoporphyrin IX silica-based nanoparticles for photodynamic therapy in vitro

RSC Advances ◽  
2014 ◽  
Vol 4 (28) ◽  
pp. 14400-14407 ◽  
Author(s):  
Juan L. Vivero-Escoto ◽  
Daniel L. Vega
Keyword(s):  
Photodynamic Therapy ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Protoporphyrin Ix ◽  
Stimuli Responsive ◽  
Therapy Efficacy ◽  
Redox Responsive

Redox-responsive silica nanoparticles, which selectively release photosensitizers inside cancer cells, enhance the photodynamic therapy efficacy in vitro.

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