Concomitant Infection of Helicobacter pylori and Intestinal Parasites in Adults Attending a Referral Centre for Parasitic Infections in North Eastern Italy

Background: Helicobacter pylori and intestinal parasites are estimated to infect with high burden worldwide. However, their concomitant infections are poorly determined in industrialized countries, such as Italy. In this study we aim at describing the presence of H. pylori as well as the proportion of coinfections with intestinal parasites among subjects who attended a referral center for tropical diseases in Northern Italy. Methods: This was a case-control study. Screening for H. pylori and parasites was performed on stool samples of 93 adults from different geographical origin (Africa, Asia, South-America, East-Europe and Italy). H. pylori infection was examined by CLIA and its cagA positivity was determined by rtPCR. Intestinal parasites (i.e., protozoa and helminths) were examined by microscopy and rtPCR. Results: Sixty-one out of 93 patients (66%) were positive to H. pylori and 31 (33%) were cagA+. Among H. pylori positives, 45 (74%) had a concomitant infection. The coinfection H. pylori–Blastocystis was the most frequent one, followed by H. pylori–E. coli. Multivariable logistic regression showed that positivity to H. pylori was associated with having a coinfection. Conclusion: Our data suggested that H. pylori and intestinal parasitic infections are fairly common in subjects who attended a referral center for tropical diseases in Northern Italy. The high rate of H. pylori infection, and especially the positivity to the virulent cagA+, should be taken into consideration in subjects undergoing screening for parasitic infections.

Download Full-text

Role of Helicobacter Pylori in Gastro-Intestinal Tract Disorders (Gitds) Among Patients in Kirkuk City, Iraq

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2555 ◽

2017 ◽

Vol 14 (3) ◽

pp. 1159-1164

Author(s):

Adil A. Abdul Razaq ◽

Chateen I. Ali Pambuk ◽

Yahya J. Salman

Keyword(s):

Helicobacter Pylori ◽

Intestinal Tract ◽

Parasitic Infection ◽

High Rate ◽

Parasitic Infections ◽

Duodenal Ulcers ◽

Bowel Diseases ◽

Stool Samples ◽

H Pylori ◽

Gastro Intestinal Tract

ABSTRACT: In recent works Helicobacter pylori (H. pylori) become a significant criteria and essential diagnostic tool for studying the etiology of gastro-intestinal tract disorders (GITDs), for this purpose a total of stool samples were collected from 266 patients from both gender ,they were aging from below one year to over than 70 years. Their compliance with epi-gastric pain, diarrhea weigh loose and other disorders. Types of GITDS were detected and classified by the gastro-enterologists in two main Hospitals and from patients attending Private clinics and medical labs in Kirkuk city. H. pylori was detected form stool samples by using rapid lateral immune-chromatography assay (RLICA). GITDs involve, H. pylori positive distributed in 132 (49.62%), followed by 74 (27.81 % ), 59(22.18 %), 7(2.63 %), 3(1.12%) and 2(0.75 %) for irritable bowel diseases, inflammatory bowel diseases, peptic ulcer, ulcerative colitis and duodenal ulcers diseases respectively. Whereas 171, (64.28%) were positive for parasitic infection, P<0.05 .Moreover other 48, (18.04 %) stool samples were non-identified it's about from the described diseases. A total of132 (49.62 %) of H. pylori positive samples were distributed in 46(38.84 %) samples for IBD versus to 41(31.81 %) for IBS and 33(25%) were positive for parasitic infections, p <0.05. According to age; highest rate 10 (55.55 %) of H. pylori was recorded among patients aging from 61 to 70 years, whom they have IBD. Whereas 7 (70 %) have IBS within age group from 41 to 50 years, P <0.05. It can be that H. pylori participate with high rate in GITDs patients particularly IBD elderly patients.

Download Full-text

Moxifloxacin Based Triple Therapy as Alternative to Standard Therapy in Helicobacter Pylori Eradication.

Anti-Infective Agents ◽

10.2174/2211352518999200925154501 ◽

2020 ◽

Vol 18 ◽

Author(s):

Mohammed Hussien Ahmed ◽

Sherief Abd-Elsalam ◽

Aya Mohammed Mahrous

Keyword(s):

Helicobacter Pylori ◽

Triple Therapy ◽

Therapy Group ◽

Low Cost ◽

High Rate ◽

Helicobacter Pylori Eradication ◽

Treatment Regimens ◽

Group A ◽

H Pylori ◽

Group B

Introduction: Helicobacter pylori eradication remains a problematic issue. We are in an urgent need for finding a treatment regimen that achieves eradication at a low cost and less side effect. Recent published results showing a high rate of resistance and with clarithromycin-based treatment regimens. The aim of the study was to compare moxifloxacin therapy and classic clarithromycin triple therapy in H. pylori eradication. Methods: This was a pilot study that enrolled 60 patients with helicobacter pylori associated gastritis. Diagnosis was done by assessment of H. pylori Ag in the stool. The patients were randomly assigned to receive either moxifloxacin based therapy (Group A), or clarithromycin based therapy (Group B) for two weeks. We stopped the treatment for another two weeks then reevaluation for cure was done. Results: 90 % of patients had negative H. pylori Ag in the stool after 2 weeks of stoppage of the treatment in group A versus 66.7 % in Group B. None of the patients in both groups had major side effects. Conclusion: Moxifloxacin-based therapy showed higher eradication power and less resistance when compared to clarithromycin triple therapy.

Download Full-text

The Magnitude and associated factors of Helicobacter pylori infection among adult dyspeptic patients attending Bokoji Hospital, Southeast Ethiopia

10.21203/rs.3.rs-25765/v1 ◽

2020 ◽

Author(s):

Dagaga Goboto Kenea ◽

Mesfin Negawo ◽

Firaol Kitila Lemessa ◽

Solomon Tejineh

Keyword(s):

Helicobacter Pylori ◽

Alcohol Drinking ◽

Intestinal Parasites ◽

Helicobacter Pylori Infection ◽

P Value ◽

Cross Sectional ◽

Gastrointestinal Complications ◽

Ulcer Disease ◽

Southeast Ethiopia ◽

H Pylori

Abstract Background: Helicobacter pylori infection is the most common chronic bacterial infection and there were approximately 4.4 billion individuals with H pylori infection worldwide. Among those, hundreds of millions of people develop peptic ulcer disease during their lifetime and still tens of millions might progress to gastric cancer. Hence, early information is very important to prevent upper gastrointestinal complications. Consequently, the current study aimed to assess the magnitude and associated risk factors of Helicobacter pylori infection among adult dyspeptic patients attending Bokoji hospital, Southeast Ethiopia.Methods: A hospital-based cross-sectional study involving 348 adult dyspeptic patients attending Bokoji hospital was carried out from July 16 to October 31, 2019. About 50 mg of fresh stool and 60 µl of capillary blood were collected from each dyspeptic patient and analyzed for detection of Helicobacter pylori antigens, presence of intestinal parasites and ABO blood grouping respectively. Data were entered using Epi Info 7 and Statistical analysis was done using SPSS version 21 and a p-value less than 0.05 was considered as statistically significant.Results: The overall magnitude of Helicobacter pylori infection was 47.7%. The magnitude of H. pylori infection was almost two times higher in patients who do not have a habit of handwashing after toilet visit than those who wash their hand's habit of handwashing after visiting toilet (AOR 2.241, 95% CI (1.410, 3.563) and alcohol drinking habit (AOR 1.796, 95% CI (1.087, 2.968). Conclusions: The magnitude of H. pylori was high in the study area and associated with handwashing habits after toilet use and alcohol drinking habits. Therefore, the community could be educated on the handwashing habit to minimize H. pylori infections.

Download Full-text

Intestinal Parasitic Infections in Mental Retardation Center of Bandar Abbas, Southern Iran

Iranian Journal of Parasitology ◽

10.18502/ijpa.v14i2.1145 ◽

2019 ◽

Author(s):

Vahedeh MOHAMMADI-MESKIN ◽

Yaghoob HAMEDI ◽

Mehrgan HEYDARI-HENGAMI ◽

Ebrahim EFTEKHAR ◽

Jebreil SHAMSEDDIN ◽

...

Keyword(s):

Intestinal Parasites ◽

Parasitic Infection ◽

Agar Plate ◽

Mentally Retarded ◽

Living Environment ◽

High Rate ◽

Parasitic Infections ◽

Blastocystis Hominis ◽

Cross Sectional ◽

Bandar Abbas

Background: The present study aimed to evaluate the prevalence of intestinal parasitic infection among mentally retarded individuals and the staff of the center in Bandar Abbas, south of Iran. Methods: This cross-sectional study was conducted in central institution for mentally retarded in Bandar Abbas, Hormozgan Province, from 2016 to 2017. A triple fecal specimen was collected from each one of the 163 participants and were evaluated using wet mount and formalin-ethyl acetate methods. Trichrome and Ziehl-Neelsen staining were used to confirm suspected cases of protozoa. As well as Baermann and Harada-Mori techniques and agar plate culture were implemented to diagnosis of Strongyloides stercoralis. Results: Overall, 163 subjects were examined including 126 mentally retarded individuals and 37 personnel. Ninety (55.2%) cases of participants were infected with at least one of the intestinal parasites, 69 (54.7%) of mentally retarded and 21 (56.7%) of personnel. Twenty-six mentally retarded individuals were infected with S. stercoralis (20.6%), Blastocystis hominis 30.2%, Entamoeba coli 25.4%, Giardia lamblia 5.6%, Enterobius vermicularis 1.6%, Hymenolepis nana 0.8% and Iodamoeba butschlii 1.6%. Twelve staff (32.4%) harbored B. hominis, E. coli 27%, S. stercoralis 2.7%, G. lamblia 10.8%, H. nana 2.7% and Endolimax nana 2.7%. Conclusion: High rate of intestinal parasites particularly, S. stercoralis compared to the most recent studies of general population. Therefore, regular screening and tracking the positive cases, disinfection of the living environment, training and financing of the staff, increasing the number of the workers, recruiting of professionals and trained personnel in these centers are suggested.

Download Full-text

Natural Competence Promotes Helicobacter pylori Chronic Infection

Infection and Immunity ◽

10.1128/iai.01042-12 ◽

2012 ◽

Vol 81 (1) ◽

pp. 209-215 ◽

Cited By ~ 31

Author(s):

Marion S. Dorer ◽

Ilana E. Cohen ◽

Tate H. Sessler ◽

Jutta Fero ◽

Nina R. Salama

Keyword(s):

Helicobacter Pylori ◽

Chronic Infection ◽

Acute Infection ◽

Host Cells ◽

High Rate ◽

Natural Competence ◽

Content Type ◽

Type Iv ◽

Competence Factor ◽

H Pylori

Animal models are important tools for studies of human disease, but developing these models is a particular challenge with regard to organisms with restricted host ranges, such as the human stomach pathogenHelicobacter pylori. In most cases,H. pyloriinfects the stomach for many decades before symptoms appear, distinguishing it from many bacterial pathogens that cause acute infection. To model chronic infection in the mouse, a human clinical isolate was selected for its ability to survive for 2 months in the mouse stomach, and the resulting strain, MSD132, colonized the mouse stomach for at least 28 weeks. During selection, thecagYcomponent of the Cag type IV secretion system was mutated, disrupting a key interaction with host cells. Increases in both bacterial persistence and bacterial burden occurred prior to this mutation, and a mixed population ofcagY+andcagYmutant cells was isolated from a single mouse, suggesting that mutations accumulate during selection and that factors in addition to the Cag apparatus are important for murine adaptation. Diversity in both alleles and genes is common inH. pyloristrains, and natural competence mediates a high rate of interstrain genetic exchange. Mutations of the Com apparatus, a membrane DNA transporter, and DprA, a cytosolic competence factor, resulted in reduced persistence, although initial colonization was normal. Thus, exchange of DNA between genetically heterogeneousH. pyloristrains may improve chronic colonization. The strains and methods described here will be important tools for defining both the spectrum of mutations that promote murine adaptation and the genetic program of chronic infection.

Download Full-text

DRUG RESISTANCE IN HELICOBACTER PYLORI

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032016000400002 ◽

2016 ◽

Vol 53 (4) ◽

pp. 215-223 ◽

Cited By ~ 19

Author(s):

Júlia Silveira VIANNA ◽

Ivy Bastos RAMIS ◽

Daniela Fernandes RAMOS ◽

Andrea VON GROLL ◽

Pedro Eduardo Almeida da SILVA

Keyword(s):

Helicobacter Pylori ◽

Digestive System ◽

Point Mutations ◽

Resistance Mechanisms ◽

High Rate ◽

Current Therapy ◽

Genetic Characteristics ◽

Inappropriate Use ◽

Worldwide Distribution ◽

H Pylori

ABSTRACT Background Helicobacter pylori has a worldwide distribution and is associated with the pathogenesis of various diseases of the digestive system. Treatment to eradicate this microorganism involves the use of a combination of antimicrobials, such as amoxicillin, metronidazole, clarithromycin, and levofloxacin, combined with proton pump inhibitors. Although the current therapy is effective, a high rate of treatment failure has been observed, mainly because of the acquisition of point mutations, one of the major resistance mechanisms developed by H. pylori. This phenomenon is related to frequent and/or inappropriate use of antibiotics. Conclusion This review reported an overview of the resistance to the main drugs used in the treatment of H. pylori, confirming the hypothesis that antibacterial resistance is a highly local phenomenon and genetic characteristics of a given population can influence which therapy is the most appropriate.

Download Full-text

Burden of Helicobacter pylori Infections and Associated Risk Factors among Women of Child Bearing Age in Addis Ababa, Ethiopia

International Journal of Chronic Diseases ◽

10.1155/2018/5183713 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10

Author(s):

Kumera Terfa Kitila ◽

Lemi Mosisa Sori ◽

Daniel Melese Desalegn ◽

Kassu Desta Tullu

Keyword(s):

Risk Factors ◽

Helicobacter Pylori ◽

Intestinal Parasites ◽

Addis Ababa ◽

Public Health Problem ◽

Child Bearing ◽

Test Kit ◽

H Pylori ◽

Associated Risk Factors ◽

Study Participants

Background. Early detection and treatment of Helicobacter pylori (H. pylori) infection in women of child bearing ages may reduce the risk of maternal health disorder. This study was conducted to determine the burden of H. pylori infections and associated risk factors among women of child bearing ages in Kolfe Keranio Subcity Woreda 9 Health Centers, Addis Ababa, Ethiopia. Methods. Facility based cross sectional study design was conducted from April to October 2015. The study recruited 195 pregnant and 137 nonpregnant women with age range of 16-40 years. Sociodemographic data of study participants were collected by structured questionnaire. Venous blood was analyzed to determine hemoglobin, H. pylori stool antigen test kit was used to assess H. pylori infection, and fresh fecal (stool) was used to examine intestinal parasites among study subjects. Data was entered and analyzed using SPSS version 19. Bivariate and multivariate logistic regression model using odds ratio (OR) at 95% confidence interval (CI) were calculated. P-value less than 0.05 was taken as statistically significant. Results. The overall burden of H. pylori infection among study participants was 29% (96/332). H. pylori infection was statistically significantly associated with pregnancy status (AOR: 1.825, CI (1.42-2.15), P=0.020), history of hyperemesis gravidarum (AOR=7.028, C.I (2.47-19.99), P=0.018), and low hemoglobin value (AOR=0.177, CI (0.083–0.379), p=0.003). There was no statistically significant association between H. pylori infection and sociodemographic characteristics and some expected risk factors like smoking, Khat chewing, alcohol drinking habit, and presence of intestinal parasites. Conclusion. In this study, H. pylori infection was still a public health problem in the study area. H. pylori infected women also had high rate of anemia compared to women who had not H. pylori infected. Hence clinician and other responsible bodies should give a special attention for women who had been infected with H. pylori. Further large case control studies are warranted to understand more the role of H. pylori, HG, and other associated risk factors.

Download Full-text

Use of PCR and culture to detect Helicobacter pylori in naturally infected cats following triple antimicrobial therapy.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.40.6.1486 ◽

1996 ◽

Vol 40 (6) ◽

pp. 1486-1490 ◽

Cited By ~ 30

Author(s):

S E Perkins ◽

L L Yan ◽

Z Shen ◽

A Hayward ◽

J C Murphy ◽

...

Keyword(s):

Helicobacter Pylori ◽

Gastric Juice ◽

Antimicrobial Therapy ◽

Developed Countries ◽

Gastric Fluid ◽

High Rate ◽

Pcr Analysis ◽

Human Populations ◽

Peptic Ulcers ◽

H Pylori

Helicobacter pylori causes gastritis and peptic ulcers and is linked to gastric cancer. Domestic cats from a commercial source were found to be naturally infected with H. pylori, and studies were undertaken to eradicate H. pylori from infected cats by using triple antimicrobial therapy. Eight cats infected with H. pylori were used in the study. Six cats received a 21-day course of oral amoxicillin, metronidazole, and omeprazole, and two cats served as controls. Two weeks and 4 weeks posttreatment (p.t.), all six treated cats were negative at several sites (saliva, gastric juice, and gastric mucosa) for H. pylori by culture. However, as determined by PCR with primers specific for the 26-kDa product, the majority of cats at 2 and 4 weeks p.t. had gastric fluid samples which were positive for H. pylori and three of three cats at 2 weeks p.t. had dental plaque which was positive for H. pylori. At 6 weeks p.t., all six cats had H. pylori-negative cultures for samples from several gastric sites taken at necropsy, and only one cat had H. pylori cultured from gastric juice. PCR analysis revealed that five of six cats had H. pylori DNA amplification products from plaque, saliva, and/or gastric fluid samples. Negative bacterial cultures for cats for which there was demonstrable PCR amplification of H. pylori DNA may reflect the inability of in vitro culture techniques to isolate small numbers of H. pylori organisms, focal colonization at sites not cultured, or a failure of the antibiotics to successfully eradicate H. pylori from extragastric sites which allowed subsequent recolonization of the stomach after cessation of therapy. Alternatively, the treatment strategy may have induced in vivo viable but nonculturable coccoid forms of H. pylori. The H. pylori cat model should allow further studies to test these hypotheses as well as the efficacies of other combined therapeutic regimens. Also, because 100% of these cats were naturally infected with H.pylori, this model should prove useful in exploring mechanisms whereby human populations in underdeveloped countries, which have H. pylori infection rates approaching 100%, have a high rate of recurrence of H. pylori infection after use of prescribed antibiotic therapies that successfully eradicate H. pylori in individuals in developed countries.

Download Full-text

APPLICATION OF STOOL ANTIGEN TEST FOR MONITORING HELICOBACTER PYLORI AMONG HUMAN IN ERBIL GOVERNORATE, KURDISTAN REGION/IRAQ

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i11.28872 ◽

2018 ◽

Vol 10 (11) ◽

pp. 49

Author(s):

Dhary Alewy Al-mashhadany

Keyword(s):

Helicobacter Pylori ◽

Urban Area ◽

Early Years ◽

High Rate ◽

Occurrence Rate ◽

Antigen Test ◽

Stool Antigen Test ◽

Stool Antigen ◽

H Pylori ◽

Rural And Urban Area

Objective: This work was connected to screen Helicobacter pylori among human in Erbil Governorate by using stool antigen test (SAT).Methods: In a clean and sterile container, three hundred stool samples were collected from both sexes during the period from July-December 2017. Samples were collected from 150 males at the rural and an urban area in equal number, similarly 150 females same areas. The collected samples were tested in Microbiology Laboratory, Department of Pathological Analysis using One-Step H. pylori Antigen Test Kit.Results: The obtained results shown that the prevalence of H. pylori in total samples were (11.3%). The rate of infection among females were (12.7%) compared to (10.0%) of male infection rate. According to the age wise of the patients (11-20, 51-60 and above 60 y) results showed that the H. pylori antigen rates were (16.3%, 11.9%, and 13.6%) respectively that mean the high rate of infection was varied. According to habitation, the high rate of H. pylori among males was 12.0% and 8.0% in rural and urban area consecutively. While the occurrence rate of H. pylori antigen among female was high 14.7% in rural area, compared to 10.7% in the female of the urban area. The proportion of H. pylori antigen rate in September, December and October were (16.0%, 14.0%, and 12.0%) respectively.Conclusion: From this study, we concluded that the prevalence of H. pylori among human in Erbil Governorate was high, and the infection takes place in the early years of life. The significance of public health risks was discussed.

Download Full-text

STUDY OF HELICOBACTER PYLORI ERADICATON RATE OF BISMUTH-CONTAINING QUADRUPLE REGIMEN IN PATENTS WITH CHRONIC GASTRITS

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2016.3.5 ◽

2016 ◽

pp. 31-35

Author(s):

Van Huy Tran ◽

Thi Minh Trieu Nguyen

Keyword(s):

Helicobacter Pylori ◽

Side Effects ◽

Chronic Gastritis ◽

Eradication Rate ◽

High Rate ◽

Rapid Urease Test ◽

Good Tolerance ◽

Central Vietnam ◽

Urease Test ◽

H Pylori

Background: Quadruple therapy with bismuth was considered as a promising regimen in the eradication of Helicobacter pylori in the population with clarithromycine- resistant high rate, but data in Vietnam was still limited. This study is aimed at determining the eradication rate of quadruple regimen and its side effects in the patients with chronic gastritis. Patients and methods: 98 patients with chronic gastritis histologically diagnosed and postive with rapid urease test were enrolled. Patients received Rabeprazole 20 mg bid, Pepto bismol 2tab bid, tetracyclin 1000 mg bid and Metronidazole 550 mg bid for 10 days. Results: Eradication rates of quadruple regimens with bismuth were 91.8% in ITT analysis and 94.7% in PP analysis. Rate of compliance was 96.9%. The side effects were minor and transient. Conclusion: Quadruple regimen with bismuth showed a high rate of H. pylori eradication and a good tolerance in patients with chronic gastritis in central Vietnam. Key words: Helicobacter pylori, quadruple regimen, bismuth

Download Full-text