scholarly journals Cytotoxicity and Gene Expression Changes of a Novel Homeopathic Antiseptic Oral Rinse in Comparison to Chlorhexidine in Gingival Fibroblasts

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3190
Author(s):  
Masako Fujioka-Kobayashi ◽  
Benoit Schaller ◽  
Michael A. Pikos ◽  
Anton Sculean ◽  
Richard J. Miron
Keyword(s):  
Clinical Studies ◽  
Collagen Synthesis ◽  
Oral Care ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Migration Assay ◽  
Fibroblast Migration ◽  
Oral Rinse ◽  
Cellular Inflammatory Response

Most available antiseptic solutions available today have strong antibacterial effects, however most also possess major cytotoxic effects on human gingival tissues. The VEGA Oral Care Recovery Kit (StellaLife), previously evaluated in clinical studies, consists of 16 active ingredients that are monographed in the Homeopathic Pharmacopeia of United States (HPUS) and recognized for their accelerated healing properties (reduction in post-op pain). The aim of this study was to compare VEGA to chlorhexidine (CHX) in vitro on gingival fibroblast viability, survival at various concentrations, migration assay, proliferation activity, expression of both regenerative growth factors as well as inflammatory markers, and collagen synthesis. A 10-fold dilution of standard CHX (0.02%) led to cell death, whereas cell viability was significantly better in the VEGA group for all tested parameters. Furthermore, VEGA also induced significantly greater fibroblast migration and proliferation. CHX negatively impacted the cellular inflammatory response of gingival fibroblasts, and also led to a reduction in collagen synthesis (50% decrease). Findings from the present study provide support from basic laboratory experiments that validate the previous clinical studies supporting the use of the VEGA oral rinse on its superior biocompatibility and wound healing properties when compared to CHX.

scholarly journals Comparison of Cytomorphometry and Early Cell Response of Human Gingival Fibroblast (HGFs) between Zirconium and New Zirconia-Reinforced Lithium Silicate Ceramics (ZLS)

2018 ◽  
Vol 19 (9) ◽  
pp. 2718 ◽  
Author(s):  
María Rizo-Gorrita ◽  
Irene Luna-Oliva ◽  
María-Ángeles Serrera-Figallo ◽  
José-Luis Gutiérrez-Pérez ◽  
Daniel Torres-Lagares
Keyword(s):  
Cellular Response ◽  
Surface Characterization ◽  
Cellular Proliferation ◽  
Lithium Silicate ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Optical Profilometry

New zirconia-reinforced lithium silicate ceramics (ZLS) could be a viable alternative to zirconium (Y-TZP) in the manufacture of implantological abutments—especially in aesthetic cases—due to its good mechanical, optical, and biocompatibility properties. Although there are several studies on the ZLS mechanical properties, there are no studies regarding proliferation, spreading, or cytomorphometry. We designed the present study which compares the surface, cellular proliferation, and cellular morphology between Y-TZP (Vita YZ® T [Vita Zahnfabrik (Postfach, Germany)]) and ZLS (Celtra® Duo [Degudent (Hanau-Wolfgang, Germany)]). The surface characterization was performed with energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), and optical profilometry. Human gingival fibroblasts (HGFs) were subsequently cultured on both materials and early cellular response and cell morphology were compared through nuclear and cytoskeletal measurement parameters using confocal microscopy. The results showed greater proliferation and spreading on the surface of Y-TZP. This could indicate that Y-TZP continues to be a gold standard in terms of transgingival implant material: Nevertheless, more in vitro and in vivo research is necessary to confirm the results obtained in this study.

scholarly journals Rinsing with L-Ascorbic Acid Exhibits Concentration-Dependent Effects on Human Gingival Fibroblast In Vitro Wound Healing Behavior

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Tatcha Chaitrakoonthong ◽  
Ruchanee Ampornaramveth ◽  
Paksinee Kamolratanakul
Keyword(s):  
Wound Healing ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Cell Viability ◽  
Real Time ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Migration ◽  
Matrix Gene

Vitamin C or L-ascorbic acid has diverse functions in the body, especially healing promotion in tissue injury via participating in the hydroxylation reactions required for collagen formation. Systemic administration of vitamin C plays an important role on gingival fibroblast proliferation and functions. Whether local or rinsing administration of vitamin C alters gingival fibroblast wound healing behavior remains unclear. The aim of this study was to investigate the rinsing effect of vitamin C on gingival fibroblast behavior utilizing an in vitro wound healing model. Primary human gingival fibroblasts isolated from gingival tissue were rinsed with medium containing various concentrations of vitamin C. The rinsing effect of vitamin C on in vitro wound healing was assessed using a scratch test assay. Cell migration, cell viability, and extracellular matrix gene expression were analyzed by transwell migration assay, MTT assay, and real-time RT-PCR, respectively. We found that rinsing with 10 or 20 µg/ml vitamin C significantly increased fibroblast migration (p≤0.05). However, no significant effect was found in the cell viability or in vitro wound healing assays. In contrast, rinsing with 50 µg/ml vitamin C significantly delayed wound closure (p≤0.05). Real-time PCR demonstrated that 50 µg/ml vitamin C significantly increased fibroblast expression of COL1, FN, IL-6, and bFGF. The data demonstrate that rinsing with vitamin C (10/20 µg/ml) accelerates fibroblast migration. However, 50 µg/ml of vitamin C increases the expression of COL1, FN, IL-6, and bFGF, which are related to fibroblast wound healing activity. Prescribing vitamin C with the appropriate duration and drug administration method should be determined to maximize its benefit.

scholarly journals Gingerly effervescent tablets: investigating the effect of cytotoxicity on gingival fibroblasts and antimicrobial properties under laboratory conditions

2019 ◽  
Vol 9 (6) ◽  
pp. 4534-4538
Keyword(s):  
Antimicrobial Properties ◽  
Live Cells ◽  
Control Group ◽  
P Value ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Laboratory Conditions ◽  
Significant Difference ◽  
Effervescent Tablet

Ginger is a medicinal plant with antioxidant, anti-bacterial, anti-inflammatory and anti-fungal properties. The shape of effervescent tablet is a new form of this product for use as a mouthwash. This study investigated the cytotoxicity and antimicrobial properties of gingerly effervescent tablets under laboratory conditions. In this study, MTT assay was done to evaluate in-vitro gingival fibroblast cytotoxicity during 24, 48 and 72 hours. In addition, the effect of antimicrobial properties on common bacterial and oral fungi was investigated. P- value was considered significantly less than 0.05. There was no significant difference between the mean of live cells in groups with 24, 48 and 72 duration (P-value = 0.071). There was no significant difference between ginger group and control group (P<0.05). Minimum inhibitory concentration (MIC) of effervescent tablet was different, ranging from 10 mg/ml for Candidas family to 20 mg/ml for Staphylococcus and 40 mg/ml for negative grams. According to the results, it can be concluded that gingerly effervescent tablets have no toxic effect after 72 hours of exposureto gingival fibroblasts. Its suitable antimicrobial properties for fungi makes it useful for the control of infection and as an ideal mouthwash.

scholarly journals Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report

2020 ◽  
Author(s):  
Barbara Sterczała ◽  
Kinga Grzech-Leśniak ◽  
Olga Michel ◽  
Witold Trzeciakowski ◽  
Kamil Jurczyszyn
Keyword(s):  
Energy Density ◽  
Preliminary Report ◽  
Human Fibroblasts ◽  
Mitochondrial Activity ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Proliferation ◽  
Gingival Fibroblasts ◽  
Healthy Donors

Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types: Nd:YAG (1064nm), infrared diode laser (980nm) and prototype led laser emitting 405, 450 and 635nm were used to irradiate fibroblasts. Thanks to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm&sup2;, 25 J/cm&sup2;, 64 J/cm&sup2;. The viability and proliferation of cells were determined using the MTT test conducted 24, 48 and 72 hours after laser irradiation. Results: The highest percentage of mitochondrial activity (MA=122.1%) was observed in the group irradiated with the 635nm laser, with an energy density of 64 J/cm&sup2; after 48 hours. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405nm laser at 25 J/cm&sup2; gave similar results to the 635 nm laser. Conclusions: The application of the 635nm and 405nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.

scholarly journals Efficacy of bioflavonoids of FLAVOBA against severe acute respiratory syndrome-coronavirus 2 in vitro

2021 ◽  
Vol 8 (2) ◽  
pp. 95-99
Author(s):  
Abdelahhad Barbour ◽  
◽  
Lynda Gail Darlington ◽  
Michelle Mendenhall ◽  
Henriette Lerner ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Oral Care ◽  
Virucidal Activity ◽  
Oral Rinse ◽  
Global Pandemic ◽  
Transmission Reduction ◽  
Oral Decontamination ◽  
Respiratory Droplets ◽  
Virucidal Efficacy

Introduction The Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) is responsible for the global pandemic of Coronavirus disease-2019 (COVID-19). Human-to-human transmission occurs mainly through the aerosolization of respiratory droplets. Improved antisepsis of human and non-human surfaces has been identified as a key feature of transmission reduction. Flavobac, a complex of soluble bioflavonoids and hydroxylated phenolic structures used in oral care products, has demonstrated efficacy to act against microorganisms. This study evaluated nasal and oral antiseptic formulations of FLAVOBAC for the virucidal activity against SARS-CoV-2. Methodology FLAVOBAC nasal antiseptic formulations and FLAVOBAC oral rinse antiseptic formulations from 1-10% concentrations were assayed for virucidal efficacy against the SARS-CoV-2 virus. SARS-CoV-2 was exposed directly to the test compound for 60 seconds or 5 minutes. Compounds were then neutralized, and the surviving virus was quantified. Results All concentrations of nasal antiseptics and oral rinse antiseptics evaluated completely inactivated the SARS-CoV-2 virus. Conclusion Nasal and oral FLAVOBAC solutions are effective at inactivating the SARS-CoV-2 virus at a variety of concentrations after 60-second or 5-minute exposure times. The formulations tested have the potential to reduce the transmission of SARS-CoV-2 if used for nasal/oral decontamination, or surface decontamination in known or suspected cases of COVID-19.

Microscopic Assay for the Phagocytotic-Collagenolytic Performance (PCP Index) of Human Gingival Fibroblasts in vitro

1978 ◽  
Vol 57 (11-12) ◽  
pp. 1003-1015 ◽  
Author(s):  
George G. Rose ◽  
Toshihiko Yajima ◽  
Charles J. Mahan
Keyword(s):  
Tissue Culture ◽  
Thin Layer ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Cover Slip ◽  
Fibroblast Cell Lines

Using 16 human gingival fibroblast cell lines from patients with periodontitis, Dilantin hyperplasia, and nonpathological gingiva, a microscopic assay was developed to quantitate the cells' ability to lyse collagen substrates. The method employs tissue culture chambers with one cover slip partially coated with a thin layer of undenatured fibrillar bovine codlagen. The assay measures the relative numbers and sizes of holes in the collagen within defined regions of the cover slips effected by the phagocytotic and collagenolytic performance (PCP) of the population of fibroblasts growing on the cover slip for 5 days. The effect on the PCP index by serum, heparin, prostaglandins, and endotoxin was evaluated.

scholarly journals Assessment of Human Gingival Fibroblast Proliferation after Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450, and 405 nm)—Preliminary Report

2021 ◽  
Vol 11 (2) ◽  
pp. 98
Author(s):  
Barbara Sterczała ◽  
Kinga Grzech-Leśniak ◽  
Olga Michel ◽  
Witold Trzeciakowski ◽  
Marzena Dominiak ◽  
...  
Keyword(s):  
Energy Density ◽  
Human Fibroblasts ◽  
Mitochondrial Activity ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Proliferation ◽  
Gingival Fibroblasts ◽  
Healthy Donors ◽  
Thiazolyl Blue Tetrazolium

Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types, Nd:YAG (1064 nm), infrared diode laser (980 nm), and prototype led laser emitting 405, 450, and 635 nm were used to irradiate the fibroblasts. Due to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm2, 25 J/cm2, 64 J/cm2. The viability and proliferation of cells were determined using the (Thiazolyl Blue Tetrazolium Blue) (MTT) test conducted 24, 48, and 72 h after laser irradiation. Results: The highest percentage of mitochondrial activity (MA = 122.1%) was observed in the group irradiated with the 635 nm laser, with an energy density of 64 J/cm2 after 48 h. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405 nm laser at 25 J/cm2 gave similar results to the 635 nm laser. Conclusions: The application of the 635 nm and 405 nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.

scholarly journals Identification of Interleukin-8-Reducing Lead Compounds Based on SAR Studies on Dihydrochalcone-Related Compounds in Human Gingival Fibroblasts (HGF-1 cells) In Vitro

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1382
Author(s):  
Katharina Schueller ◽  
Joachim Hans ◽  
Stefanie Pfeiffer ◽  
Jessica Walker ◽  
Jakob P. Ley ◽  
...  
Keyword(s):  
Cell Model ◽  
Periodontal Diseases ◽  
Oral Care ◽  
Interleukin 8 ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Periodontal Inflammation ◽  
Anti Inflammatory ◽  
Related Compounds

Background: In order to identify potential activities against periodontal diseases, eighteen dihydrochalcones and structurally related compounds were tested in an established biological in vitro cell model of periodontal inflammation using human gingival fibroblasts (HGF-1 cells). Methods: Subsequently to co-incubation of HGF-1 cells with a bacterial endotoxin (Porphyromonas gingivalis lipopolysaccharide, pgLPS) and each individual dihydrochalcone in a concentration range of 1 µM to 100 µM, gene expression of interleukin-8 (IL-8) was determined by qPCR and cellular interleukin-8 (IL-8) release by ELISA. Results: Structure–activity analysis based on the dihydrochalcone backbone and various substitution patterns at its aromatic ring revealed moieties 2′,4,4′,6′-tetrahydroxy 3-methoxydihydrochalcone (7) to be the most effective anti-inflammatory compound, reducing the pgLPS-induced IL-8 release concentration between 1 µM and 100 µM up to 94%. In general, a 2,4,6-trihydroxy substitution at the A-ring and concomitant vanilloyl (4-hydroxy-3-methoxy) pattern at the B-ring revealed to be preferable for IL-8 release inhibition. Furthermore, the introduction of an electronegative atom in the A,B-linker chain led to an increased anti-inflammatory activity, shown by the potency of 4-hydroxybenzoic acid N-vanillylamide (13). Conclusions: Our data may be feasible to be used for further lead structure designs for the development of potent anti-inflammatory additives in oral care products.

scholarly journals Drug-induced gingival hyperplasia: An in vitro study using amlodipine and human gingival fibroblasts

2019 ◽  
Vol 33 ◽  
pp. 205873841982774 ◽  
Author(s):  
Dorina Lauritano ◽  
Marcella Martinelli ◽  
Alessandro Baj ◽  
Giada Beltramini ◽  
Valentina Candotto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Responses ◽  
Human Gingival Fibroblasts ◽  
Gingival Tissue ◽  
Gingival Fibroblast ◽  
Gingival Hyperplasia ◽  
Gingival Fibroblasts ◽  
Drug Induced ◽  
Gingival Overgrowth

Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast’s function in gingival overgrowth. To determine whether amlodipine alters the inflammatory responses, we investigated its effects on gingival fibroblast gene expression as compared with untreated cells. Fragments of gingival tissue of healthy volunteers (11 years old boy, 68 years old woman, and 20 years old men) were collected during operation. Gene expression of 29 genes was investigated in gingival fibroblast cell culture treated with amlodipine, compared with untreated cells. Among the studied genes, only 15 ( CCL1, CCL2D, CCL5, CCL8, CXCL5, CXCL10, CCR1, CCR10, IL1A, IL1B, IL5, IL7, IL8, SPP1, and TNFSF10) were significantly deregulated. In particular, the most evident overexpressed genes in treated cells were CCR10 and IL1A. These results seem to indicate a possible role of amlodipine in the inflammatory response of treated human gingival fibroblasts.

Sign in / Sign up

Export Citation Format

Share Document