scholarly journals A Proteomic Analysis of Discolored Tooth Surfaces after the Use of 0.12% Chlorhexidine (CHX) Mouthwash and CHX Provided with an Anti-Discoloration System (ADS)

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4338
Author(s):  
Stefania Bergamini ◽  
Elisa Bellei ◽  
Luigi Generali ◽  
Aldo Tomasi ◽  
Carlo Bertoldi
Keyword(s):  
Proteomic Analysis ◽  
Supportive Therapy ◽  
Cell Permeability ◽  
Tooth Surface ◽  
Two Dimensional Gel Electrophoresis ◽  
Bone Surgery ◽  
Tooth Surfaces ◽  
Plaque Control ◽  
Bacterial Plaque ◽  
Therapy Protocol

Chlorhexidine (CHX) is considered the gold standard for the chemical control of bacterial plaque and is often used after surgical treatment. However, CHX employment over an extended time is responsible for side effects such as the appearance of pigmentations on the teeth and tongue; the discoloration effects are less pronounced when using a CHX-based mouthwash with added an anti-discoloration system (ADS). The aim of this study was to evaluate, using one- and two-dimensional gel electrophoresis combined with mass spectrometry, the possible proteomic changes induced by CHX and CHX+ADS in the supragingival dental sites susceptible to a discoloration effect. The tooth surface collected material (TSCM) was obtained by curettage after resective bone surgery from three groups of patients following a supportive therapy protocol in which a mechanical control was combined with placebo rinses or CHX or a CHX+ADS mouthwash. The proteomic analysis was performed before surgery (basal conditions) and four weeks after surgery when CHX was used (or not) as chemical plaque control. Changes in the TSCM proteome were only revealed following CHX treatment: glycolytic enzymes, molecular chaperones and elongation factors were identified as more expressed. These changes were not detected after CHX+ADS treatment. An ADS could directly limit TSCM forming and also the CHX antiseptic effect reduces its ability to alter bacterial cell permeability. However, Maillard’s reaction produces high molecular weight molecules that change the surface properties and could facilitate bacterial adhesion.

scholarly journals Plaque accumulation and Streptococcus mutans levels around self-ligating bracket clips and elastomeric modules: A randomized controlled trial

2015 ◽  
Vol 5 ◽  
pp. 97-102
Author(s):  
Dhaval Fadia ◽  
Meghna Vandekar ◽  
Nikhilesh Vaid ◽  
Viraj Doshi
Keyword(s):  
Streptococcus Mutans ◽  
Bacterial Colonization ◽  
Controlled Trial ◽  
Tooth Surface ◽  
Time Intervals ◽  
Orthodontic Bracket ◽  
Colony Forming Units ◽  
Orthodontic Patients ◽  
Plaque Control ◽  
Bacterial Plaque

Aim To determine the effect of two different ligating systems that is, elastomeric modules and self-ligating (SL) bracket systems (Smartclip - 3M Unitek) with respect to harboring bacterial plaque in fixed orthodontic treatment. Objectives To assess, evaluate, and compare the amount of plaque accumulation and Streptococcus mutans colonization around elastomeric ligation and SL clips in the smart clip appliance. Materials and Methods A total of 111 orthodontic patients scheduled for fixed orthodontic treatments were selected for this split maxillary arch study. All the patients were bonded with smart-clip (3M Unitek) SL brackets, and the wire was placed into the bracket slots, on the randomly selected hemi arch, elastomeric modules were placed for the study to be conducted. Microbial and periodontal plaque accumulation was recorded at 3-time intervals post ligation. Plaque index-by Silness and Loe, modified Quigely Hein index, bleeding on probing were evaluated, and biofilm was collected from the tooth surface after 30 days and placed in petri dishes containing Mitis Salivarius agar for bacterial culturing. Result It was observed that the side where ligation was done with elastomeric modules accumulated more plaque and increase in S. mutans colony forming units as compared to the side without external ligation (P < 0.05). Conclusion Reduced bacterial colonization and better plaque control was seen with SL orthodontic bracket appliance system as compared to conventional ligation method.

Estimation of Gear Friction Coefficient using Directional Parameter of Tooth Surface

2021 ◽  
pp. 1-27
Author(s):  
Junichi Hongu ◽  
Ryohei Horita ◽  
Takao Koide
Keyword(s):  
Friction Coefficient ◽  
Contact Surface ◽  
Gear Tooth ◽  
Correction Term ◽  
Tooth Surface ◽  
Oil Film ◽  
Tooth Surfaces ◽  
Frictional Coefficients ◽  
Finishing Processes ◽  
The Relationship

Abstract This study proposes a modification of the Matsumoto equation using a directional parameter of tooth surfaces to adapt various gear finishing processes. The directional parameters of a contact surface, which affect oil film formations, have been discussed in the field of tribology; but this effect has been undetermined on the meshing gear tooth surfaces having directional machining marks. Thus, this paper investigates the relationship between the gear frictional coefficients and the directional parameters (based on ISO25178) of their tooth surfaces with the various finishing processes; and modifies the Matsumoto equation by introducing a new directional parameter to augment the various gear finishing processes. Our findings indicate that through optimizing the coefficient of the correction term the include the new directional parameter, the calculated friction values using the modified Matsumoto equation correlate more highly to the experimental friction values than that using the unmodified Matsumoto equation.

scholarly journals Exopolysaccharides Produced by Streptococcus mutans Glucosyltransferases Modulate the Establishment of Microcolonies within Multispecies Biofilms

2010 ◽  
Vol 192 (12) ◽  
pp. 3024-3032 ◽  
Author(s):  
H. Koo ◽  
J. Xiao ◽  
M. I. Klein ◽  
J. G. Jeon
Keyword(s):  
Streptococcus Mutans ◽  
Extracellular Polysaccharide ◽  
Fluorescence Labeling ◽  
Tooth Surface ◽  
Bacterial Cells ◽  
Actinomyces Naeslundii ◽  
Multispecies Biofilm ◽  
Tooth Surfaces ◽  
Glucan Synthesis

ABSTRACT Streptococcus mutans is a key contributor to the formation of the extracellular polysaccharide (EPS) matrix in dental biofilms. The exopolysaccharides, which are mostly glucans synthesized by streptococcal glucosyltransferases (Gtfs), provide binding sites that promote accumulation of microorganisms on the tooth surface and further establishment of pathogenic biofilms. This study explored (i) the role of S. mutans Gtfs in the development of the EPS matrix and microcolonies in biofilms, (ii) the influence of exopolysaccharides on formation of microcolonies, and (iii) establishment of S. mutans in a multispecies biofilm in vitro using a novel fluorescence labeling technique. Our data show that the ability of S. mutans strains defective in the gtfB gene or the gtfB and gtfC genes to form microcolonies on saliva-coated hydroxyapatite surfaces was markedly disrupted. However, deletion of both gtfB (associated with insoluble glucan synthesis) and gtfC (associated with insoluble and soluble glucan synthesis) is required for the maximum reduction in EPS matrix and biofilm formation. S. mutans grown with sucrose in the presence of Streptococcus oralis and Actinomyces naeslundii steadily formed exopolysaccharides, which allowed the initial clustering of bacterial cells and further development into highly structured microcolonies. Concomitantly, S. mutans became the major species in the mature biofilm. Neither the EPS matrix nor microcolonies were formed in the presence of glucose in the multispecies biofilm. Our data show that GtfB and GtfC are essential for establishment of the EPS matrix, but GtfB appears to be responsible for formation of microcolonies by S. mutans; these Gtf-mediated processes may enhance the competitiveness of S. mutans in the multispecies environment in biofilms on tooth surfaces.

scholarly journals Isolation of Candida species in children and their biofilm-forming ability on nano-composite surfaces

2020 ◽  
Vol 1 (1) ◽  
pp. 40-52
Author(s):  
Harina Akila Che Hussin ◽  
Nadiah Arshad ◽  
Siti Nor Humaira Nor Azemi ◽  
Raja Izzatun Nisa Raja Sahrul Hishan ◽  
Wan Nur Fatihah Wan Mohd Kamaluddin ◽  
...  
Keyword(s):  
Dental Caries ◽  
Oral Cavity ◽  
Buccal Mucosa ◽  
Candida Species ◽  
Soft Tissues ◽  
Tooth Surface ◽  
Nano Composites ◽  
Candida Spp ◽  
Nano Composite ◽  
Tooth Surfaces

Candida species including Candida albicans, Candida krusei and Candida glabrata are opportunistic microorganisms that inhabit oral cavity. The objective of this study is to determine the effect of dental caries on Candida spp. biofilm-forming ability on nano- composite with the hypothesis that dental caries enhances the colonization of Candida spp. To assess Candida spp. colonisation in the oral cavity of the paediatric patient, samples were obtained from 30 subjects aged five to six years old from Kuantan, Pahang, Malaysia. The samples were collected from buccal mucosa, palate and tooth surfaces using sterile swabs. 10 mL of patient’s saliva suspension was also collected. Following that, the samples were inoculated on CHROMagar and incubated for 24 h at 37 ºC. Candida biofilm of caries isolate C. albicans (HNFC2), and C. albicans ATCC 32354 were developed on three different types of nano-composites. The study showed that no C. albicans was isolated from the caries-free oral cavity while 76% of children with caries possessed Candida spp. 65% of the yeasts were isolated from the tooth surface. Only 35% of the total isolates were obtained from soft tissues, including palatal and buccal mucosa. C. albicans is the most isolated Candida spp. with 82% and 67% of the yeast were obtained from the tooth surface and buccal mucosa, respectively. Besides, HNFC2 significantly colonised the nano- composites more than the ATCC (P < 0.05). In the comparison of the three types of nano- composites, nano-hybrid-based containing pre-polymerised filler (cB) exhibited the least C. albicans HNFC2 cells colonisation with 7.7 x 10³ cells mL-1. In contrast, the nano-composite that contained bulk-filled nanohybrid (cC) was the most colonised with 14.3 x 10³ cells mL-1. In conclusion, dental caries enhances the colonization of Candida spp. in children's oral cavity, and that caries isolate form more biofilm on nano-composites compared to the lab strain C. albicans.

scholarly journals Gingival Inflammation in the Presence of Bacterial Plaque in Women in the First and Second Trimester of Pregnancy

2020 ◽  
Vol 2 (1) ◽  
pp. 46-52
Author(s):  
Juan Daniel Argüello Torres ◽  
Geoconda Luzardo
Keyword(s):  
Oral Hygiene ◽  
Pregnant Patient ◽  
First Trimester ◽  
Hormonal Changes ◽  
Second Trimester ◽  
Gingival Inflammation ◽  
Plaque Control ◽  
Bacterial Plaque ◽  
Transversal Study ◽  
Oral Hygiene Habits

Problem: Pregnancy hormonal changes in addition to the low control of the dental plaque generate gingivitis during the first and second trimester of the pregnancy. Objective: This study’s objective was to identify the levels of gingival inflammation having place in the first and second trimester of the pregnancy in relation to a low plaque control. Materials and Methods: This was an analytic descriptive transversal study in which 73 pregnant women that went to their monthly check to the “Centro de Salud Ministerial del Cantón Samborondón” during June and July of the year 2014 were tested. 32 of them were on their first trimester and the 41 left, on their second trimester of pregnancy. They were tested by the recollection in a document of information about their oral hygiene habits, oral hygiene indices, gingival inflammation and the presence of bacterial plaque. Results: 100% of the woman had gingivitis, from which 69.86% presented a moderate type (30.14% in the first and 39.73% in the second trimester). In the oral hygiene examination, 76.71% obtained a regular grade, from which 38.36% showed up in both trimesters. Finally, we found bacterial plaque in 67% of the patients with a range between 50.1-75%, being the 30% in the first and the 37% in the second trimester of pregnancy. Conclusion: It was found gingival inflammation in presence of bacteria plaque in every pregnant patient, showing a slightly higher intensity in the second trimester of pregnancy.

The Role of Toothbrushing in the Prevention and Treatment of Periodontal Disease: Personal Experience of Both Clinical and Experimental Observation for More than Thirty Years

1988 ◽  
Vol 2 (2) ◽  
pp. 204-208 ◽  
Author(s):  
J. Ishikawa
Keyword(s):  
Periodontal Disease ◽  
Personal Experience ◽  
Experimental Studies ◽  
Food Habit ◽  
Tooth Surface ◽  
Treatment And Prevention ◽  
Oral Environment ◽  
Systemic Factors ◽  
Bacterial Plaque

There have been dramatic developments in the theory and therapy of periodontal disease in the last few decades. This paper focuses on the role of toothbrushing in the treatment and prevention of periodontal disease, based on the author's personal experience gained from both clinical and experimental observations for more than 30 years. Even in the recent past, periodontal disease was considered to be untreatable because of various misconceptions regarding its etiology. Attention was concentrated mainly on systemic factors. As a result, periodontallyinvolved teeth were extracted mostly due to lack of technical know-how. The author devoted his primary investigative efforts to systemic factors and found that those were only minimally significant. Later, after using wild and captive monkeys to make extensive experimental studies on local factors, he was convinced that food habit (hard, fibrous, or soft) contributes directly to the etiology of periodontal disease which is restricted in the oral environment itself. Today it is well-established that accumulation of bacterial plaque on the tooth surface is the most important single factor responsible for periodontal disease, and systemic influence can merely modify the condition. Therefore, the accumulated plaque should be mechanically removed by toothbrushing. The essence of mechanical toothbrushing is not only to remove the plaque but also to compensate for the mechanical stimulation of the gingiva (gingival massage), which is lacking with modern soft food. This lecture reviews the effect of methodical toothbrushing obtained from both clinical and experimental studies in animals.

scholarly journals Minimization of Deviations of Gear Real Tooth Surfaces Determined by Coordinate Measurements

1993 ◽  
Vol 115 (4) ◽  
pp. 995-1001 ◽  
Author(s):  
F. L. Litvin ◽  
C. Kuan ◽  
J. C. Wang ◽  
R. F. Handschuh ◽  
J. Masseth ◽  
...  
Keyword(s):  
Linear Equations ◽  
Least Square Method ◽  
Least Square ◽  
Tooth Surface ◽  
Hypoid Gear ◽  
Overdetermined System ◽  
Entire Surface ◽  
First Order ◽  
Tooth Surfaces ◽  
Coordinate Measurements

The deviations of a gear’s real tooth surface from the theoretical surface are determined by coordinate measurements at the grid of the surface. A method has been developed to transform the deviations from Cartesian coordinates to those along the normal at the measurement locations. Equations are derived that relate the first order deviations with the adjustment to the manufacturing machine tool settings. The deviations of the entire surface are minimized. The minimization is achieved by application of the least-square method for an overdetermined system of linear equations. The proposed method is illustrated with a numerical example for hypoid gear and pinion.

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