scholarly journals Metabolite Profiling and Classification of Highbush Blueberry Leaves under Different Shade Treatments

Metabolites ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 79
Author(s):  
Yaqiong Wu ◽  
Hao Yang ◽  
Zhengjin Huang ◽  
Chunhong Zhang ◽  
Lianfei Lyu ◽  
...  

Blueberry belongs to the genus Vaccinium L. in the Ericaceae and is an economically important shrub that produces small berries that are rich in nutrients. There were differences in the appearance of blueberry leaves under different shade treatments. To explore the differences in metabolites in blueberry leaves under different shading treatments, nontargeted liquid chromatography–mass spectrometry (LC–MS) metabonomic analysis was performed. Different shade intensities resulted in significant differences in the contents of metabolites. A total of 6879 known metabolites were detected, including 750 significantly differentially expressed metabolites, including mainly lipids and lipid-like molecules and phenylpropanoid and polyketide superclass members. Based on a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the flavone and flavonol biosynthesis pathways were the most significantly enriched. The results of this study provide a reference and scientific basis for the establishment of a high-quality and high-yield shaded blueberry cultivation system.

Author(s):  
Peirong Li ◽  
Xinru Li ◽  
Wei Wang ◽  
Xiaoling Tan ◽  
Xiaoqi Wang ◽  
...  

Abstract The oriental armyworm, Mythimna separata (Walker) is a serious pest of agriculture that does particular damage to Gramineae crops in Asia, Europe, and Oceania. Metamorphosis is a key developmental stage in insects, although the genes underlying the metamorphic transition in M. separata remain largely unknown. Here, we sequenced the transcriptomes of five stages; mature larvae (ML), wandering (W), and pupation (1, 5, and 10 days after pupation, designated P1, P5, and P10) to identify transition-associated genes. Four libraries were generated, with 22,884, 23,534, 26,643, and 33,238 differentially expressed genes (DEGs) for the ML-vs-W, W-vs-P1, P1-vs-P5, and P5-vs-P10, respectively. Gene ontology enrichment analysis of DEGs showed that genes regulating the biosynthesis of the membrane and integral components of the membrane, which includes the cuticular protein (CP), 20-hydroxyecdysone (20E), and juvenile hormone (JH) biosynthesis, were enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that DEGs were enriched in the metabolic pathways. Of these DEGs, thirty CP, seventeen 20E, and seven JH genes were differentially expressed across the developmental stages. For transcriptome validation, ten CP, 20E, and JH-related genes were selected and verified by real-time PCR quantitative. Collectively, our results provided a basis for further studies of the molecular mechanism of metamorphosis in M. separata.


2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 428.3-429
Author(s):  
Y. Liu ◽  
Y. Huang ◽  
Q. Huang ◽  
Z. Huang ◽  
Z. Li ◽  
...  

Background:The pathogeneses of the joint diseases rheumatoid arthritis (RA), axial spondyloarthritis (axSpA), gout, and osteoarthritis (OA) are still not fully elucidated. Exosomes in synovial fluid (SF) has a critical role in the pathogenesis of arthritis. None of study has compared the proteomics of SF-derived exosomes in RA, axSpA, gout and OA.Objectives:To compare the proteomics of SF-derived exosomes in RA, axSpA, gout and OA based on tandem mass tags (TMT) labeled quantitative proteomics technique.Methods:SF-derived exosomes was isolated from RA, axSpA, gout and OA patients by the Exoquick kit combined ultracentrifugation method. TMT labeled quantitative proteomics technique was used to compare the proteomics of SF-derived exosomes. Volcano plot, hierarchical cluster, Gene Ontologies (GO), Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted.Results:A total of 1678 credible proteins were detected. With the cut off criteria of |log2 (fold-change)| ≥1.2 and p-value <0.05, 267 (140 up-regulated and 127 down-regulated)differential proteins were found in OA vs gout, 291 (179 and 112) in axSpA vs OA, 515 (109 and 406) in RA vs axSpA, 298 (191 and 107) in axSpA vs gout, 462 (160 and 302) in RA vs gout, 536 (170 and 366) in RA vs OA. GO analysis showed that the biological progress of differential proteins were mainly enriched in the “immune response”. Regarding the molecular function, the differential proteins mainly mediated “antigen binding”. GO analysis of the cellular components indicated that most proteins were annotated as “extracellular exosomes”. KEGG pathway analysis demonstrated differential proteins were significantly enriched in “complement and coagulation cascades”. The hierarchical cluster analysis of the differential proteins in the four groups showed that Lysozyme C and Keratin were more abundant in gout, Hemoglobin and Actin-related protein 2/3 complex subunit 3 in OA, Sodium/potassium-transporting ATPase subunit alpha-1 and Immunoglobulin heavy constant delta in axSpA, Pregnancy zone protein and Stromelysin-1 in RA.Conclusion:The protein profiles of SF-derived exosomes in RA, axSpA, gout and OA patients were different. The differential proteins were the potential biomarkers of RA, axSpA, gout and OA.References:[1]Cretu D, Diamandis E P, Chandran V. Delineating the synovial fluid proteome: recent advancements and ongoing challenges in biomarker research.[J]. Critical reviews in clinical laboratory sciences, 2013,50(2):51-63.[2]McArdle A J, Menikou S. What is proteomics?[J]. Archives of disease in childhood. Education and practice edition, 2020.Figure 1.The hierarchical cluster analysis of differential proteins in axSpA, OA, Gout and RA.Disclosure of Interests:None declared


2020 ◽  
Vol 11 ◽  
Author(s):  
Kong Jie ◽  
Wang Feng ◽  
Zhao Boxiang ◽  
Gong Maofeng ◽  
Zhang Jianbin ◽  
...  

The arteriovenous fistula (AVF) is the first choice for vascular access for hemodialysis of renal failure patients. Venous remodeling after exposure to high fistula flow is important for AVF to mature but the mechanism underlying remodeling is still unknown. The objective of this study is to identify the molecular mechanisms that contribute to venous remodeling after AVF. To screen and identify the differentially expressed genes (DEGs) that may involve venous remodeling after AVF, we used bioinformatics to download the public microarray data (GSE39488) from the Gene Expression Omnibus (GEO) and screen for DEGs. We then performed gene ontology (GO) function analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and gene set enrichment analysis (GSEA) for the functional annotation of DEGs. The protein-protein interaction (PPI) network was constructed and the hub genes were carried out. Finally, we harvested 12 normal vein samples and 12 AVF vein samples which were used to confirm the expressions of the hub genes by immunohistochemistry. A total of 45 DEGs were detected, including 32 upregulated and 13 downregulated DEGs. The biological process (BP) of the GO analysis were enriched in the extrinsic apoptotic signaling pathway, cGMP-mediated pathway signaling, and molting cycle. The KEGG pathway analysis showed that the upregulated DEGs were enriched in glycosaminoglycan biosynthesis and purine metabolism, while the downregulated DEGs were mainly enriched in pathways of glycosaminoglycan biosynthesis, antifolate resistance, and ABC transporters. The GSEA analysis result showed that the top three involved pathways were oxidative phosphorylation, TNFA signaling via NF-K B, and the inflammatory response. The PPI was constructed and the hub genes found through the method of DMNC showed that INHBA and NR4A2 might play an important role in venous remodeling after AVF. The integrated optical density (DOI) examined by immunohistochemistry staining showed that the expression of both INHBA and NR4A2 increased in AVF compared to the control group. Our research contributes to the understanding of the molecular mechanism of venous remodeling after exposure to high fistula flow, which may be useful in treating AVF failure.


2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Xianzhi Shi ◽  
Dazhao Song ◽  
Ziwei Qian

AbstractCoal and gas outbursts are the result of several geological factors related to coal seam gas (coal seam gas pressureTo classify the outburst hazard level of a coal seam by means of statistical methods, this study considered the geological parameters of coal seam gas and statistical data on the amount of material involved in coal outbursts. Through multivariate regression analysis, a multivariate regression equation between the outburst coal quantity andUsing a significance evaluation of the aforementioned factors, the relative contributions of the gas-related geological parameters to the outburst hazard level of a coal seam were found to follow the orderThis work provides a scientific basis for evaluating the outburst hazard level of a coal seam and adopting feasible and economical outburst-prevention measures.


2017 ◽  
Vol 33 (2) ◽  
pp. 137-140 ◽  
Author(s):  
Antonino Carbone ◽  
Annunziata Gloghini

“Ne è passata di acqua sotto i ponti.” It has been a long time since the diagnosis of Hodgkin lymphoma (HL) was exclusively based on the detection of typical Reed-Sternberg cells and the recognition of the characteristic morpho-histological background, as well as on the pathologist’s skill. The discovery of immunologic, molecular genetic and virologic biomarkers has provided an objective contribution to the diagnosis and a scientific basis for a modern classification of HL. Recent updates have clarified the nature of the so-called nodular lymphocyte predominant HL and its link to the T-cell/histiocyte-rich large B-cell lymphomas as well as its relationship with the lymphocyte-rich subset of classical HL (CHL). Molecular virology studies assessed a role for the Epstein-Barr virus in the pathogenesis of a fraction of CHL of the general population, and virtually in all cases of CHL occurring in people infected by HIV. Finally, immunologic and genetic findings corroborated the existence of grey zone lymphomas at the edges of CHL. Overall, these advances provided additional and useful information to address the treatment of patients affected by HL.


2018 ◽  
Vol 5 (1) ◽  
pp. 170907 ◽  
Author(s):  
Dejun Ji ◽  
Bo Yang ◽  
Yongjun Li ◽  
Miaoying Cai ◽  
Wei Zhang ◽  
...  

The high-quality brush hair, or Type III brush hair, is coarse hair but with a tip and little medulla, which uniquely grows in the cervical carina of Chinese Haimen goat ( Capra hircus ). To unveil the mechanism of the formation of Type III brush hair in Haimen goats, transcriptomic RNAseq technology was used for screening of differentially expressed genes (DEGs) in the skin samples of the Type III and the non-Type III hair goats, and these DEGs were analysed by KEGG pathway analysis. The results showed that a total of 295 DEGs were obtained, mainly from three main functional types: cellular component, molecular function and biological process. These DEGs were mainly enriched in three KEGG pathways, such as protein processing in endoplasmic reticulum, MAPK, and complement and coagulation cascades. These DEGs gave hints to a possible mechanism, under which heat stress possibly initiated the formation. The study provided some useful biological information, which could give a new view about the roles of certain factors in hair growth and give hints on the mechanism of the formation of the Type III brush hair in Chinese Haimen goat.


2019 ◽  
Vol 21 (4(73)) ◽  
pp. 8-21
Author(s):  
B.V. BURKYNSKYI ◽  
O.V. NIKISHYNA

Topicality. Determination of the nature, internal content, types and contradictions of economic interests of the subjects of the logistics chains of commodity markets is essential for their effective functioning in the system of the national economy. In the process of logistical interactions, market entities form a tiered system of links based on economic partnerships and trade-offs. This helps to balance the economic interests and to form a common interest in the integrated chain. The economic interests of market chains` subjects will have been emerging, interacting and realizing in the institutional system of the market, which necessitates new research into the institutional nature of interests. Aim and tasks. The purpose of the article is to substantiate the institutional nature of the economic interests of the subjects of the logistics chains of commodity markets and the typology of the main contradictions between them, as a scientific basis for development the mechanisms for balancing interests of market chains` subjects. During the research the following methods were used:dialectical, theoretical generalization and comparison, graphical, structural-logical method. Research results. In the context of institutional theory, the essence and nature of economic interests of the subjects of logistics chains of commodity markets were substantiated by the authors. The main stages of the formation of economic l interests with the emphasis on their institutionalization, namely transformation into the norm of rational behavior in the commodity market were determined. The substantive characteristics of the economic interests of the entities in the logistics chains, which reveal their institutional nature, were generalized. It is emphasized that in Ukraine there is a partial realization of the economic interests of the state, consumers, small and medium-sized entities, while corporate structures and international associations generally receive full realization of interests in commodity market chains. The classification of economic interests of subjects of market logistics chains by subject and institutional characteristics has been further developed. The authors have developed a typology of contradictions of economic interests in internal and external dimension and substantiated the essence of the main types of contradictions. Conclusion. The scientific novelty of the research is the development of the theoretical foundations of market logistics in the part of substantiation of the institutional nature of economic interests of subjects of logistics chains of commodity markets, the development of classification of economic interests, the development of typology of the main internal and external contradictions of participants of logistics chains. The applied value of the obtained results is determined by the possibility of their use by different institutions as a scientific basis for the development of methodological bases for assessing the level of contradictions of interests of market chains` subjects, as well as new mechanisms for balancing economic interests, in particular institutional ones.


Author(s):  
Руслан Олександрович Костирко ◽  
Денис Вікторович Прозоров

Formulation of problem. Disclosing information about the social responsibility reguires the formation of social accounting model and the justification of specific tools that allow to reflect incomes and expenses that are due to socially responsible activity of enterprise. The aim of the research is scientific basis for preconditions, underlying assumptions, priority directions oforganization of social accounting. The object of the research is the process of accountingfor social responsibility. Methods, used in research: scientific generalization, logical and informative, induction, deduction, analysis. The hypothesis of the research is the assumption that social accounting should be oriented to disclosing information regarding socially responsible activity for different stakeholders and formation of integrated reporting. The statement of basic materials. The increasing social responsibility of enterprises specifies the necessity of the transformation of accounting in order to give transparent information about social and ecological activity for different groups of users, as well as the formation of integrated reporting. The base of development ofsocial accountingmethodology is concept of social responsibility, sustainable development, creation of value, object and classification of socially responsible expenses are specified. It is proved that the model of combined socially oriented accounting that includes the parallel reflection business operations in accounting that are due to social activity based on additional system of analytical accounts, the formation of indicators of financial and social reporting is rational. The classification of objects in the context of realization of concepts of value and socially responsible expenses is proposed in order to organize to rationally organize case -by -case social accounting. The originality and practical significance of the research are determined by the development of underlying assumptions of the development of social accounting methodology, oriented to ensuring social responsibility of the enterprise and scientific and methodical recommendations for the classification of socially responsible expenses. Conclusions and perspectives of further research. Taking into account the need sofdifferent stakeholders for disclosingin formation about effectiveness of the economic, social and ecological activity of entities, further researches will be focused on elucidation of the methodical basis of the organization of accounting for socially responsible expenses .


2015 ◽  
Vol 78 (8) ◽  
pp. 2007-2022 ◽  
Author(s):  
Charlotte Simmler ◽  
Jeffrey R. Anderson ◽  
Laura Gauthier ◽  
David C. Lankin ◽  
James B. McAlpine ◽  
...  
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