scholarly journals Polymeric Nanowires for Diagnostic Applications

Micromachines ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 225 ◽  
Author(s):  
Hendrik Hubbe ◽  
Eduardo Mendes ◽  
Pouyan Boukany
Keyword(s):  
Electrical Signal ◽  
Considerable Progress ◽  
Detection Methods ◽  
Chemical Modifications ◽  
Single Wire ◽  
Related Research ◽  
Disordered Structures ◽  
Functional Part ◽  
Diagnostic Applications ◽  
Polymer Nanowires

Polymer nanowire-related research has shown considerable progress over the last decade. The wide variety of materials and the multitude of well-established chemical modifications have made polymer nanowires interesting as a functional part of a diagnostic biosensing device. This review provides an overview of relevant publications addressing the needs for a nanowire-based sensor for biomolecules. Working our way towards the detection methods itself, we review different nanowire fabrication methods and materials. Especially for an electrical signal read-out, the nanowire should persist in a single-wire configuration with well-defined positioning. Thus, the possibility of the alignment of nanowires is discussed. While some fabrication methods immanently yield an aligned single wire, other methods result in disordered structures and have to be manipulated into the desired configuration.

scholarly journals Applications of Electronic-Nose Technologies for Noninvasive Early Detection of Plant, Animal and Human Diseases

Chemosensors ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 45 ◽  
Author(s):  
Alphus Dan Wilson
Keyword(s):  
Electronic Nose ◽  
Gas Sensing ◽  
Sensor Arrays ◽  
Human Diseases ◽  
Detection Methods ◽  
Research Progress ◽  
Disease Detection ◽  
New Information ◽  
Disease Diagnostics ◽  
Diagnostic Applications

The development of electronic-nose (e-nose) technologies for disease diagnostics was initiated in the biomedical field for detection of biotic (microbial) causes of human diseases during the mid-1980s. The use of e-nose devices for disease-diagnostic applications subsequently was extended to plant and animal hosts through the invention of new gas-sensing instrument types and disease-detection methods with sensor arrays developed and adapted for additional host types and chemical classes of volatile organic compounds (VOCs) closely associated with individual diseases. Considerable progress in animal disease detection using e-noses in combination with metabolomics has been accomplished in the field of veterinary medicine with new important discoveries of biomarker metabolites and aroma profiles for major infectious diseases of livestock, wildlife, and fish from both terrestrial and aquaculture pathology research. Progress in the discovery of new e-nose technologies developed for biomedical applications has exploded with new information and methods for diagnostic sampling and disease detection, identification of key chemical disease biomarkers, improvements in sensor designs, algorithms for discriminant analysis, and greater, more widespread testing of efficacy in clinical trials. This review summarizes progressive advancements in utilizing these specialized gas-sensing devices for numerous diagnostic applications involving noninvasive early detections of plant, animal, and human diseases.

scholarly journals It’s the Little Things (in Viral RNA)

mBio ◽  
2020 ◽  
Vol 11 (5) ◽  
Author(s):  
Jiří František Potužník ◽  
Hana Cahová
Keyword(s):  
Mass Spectrometry ◽  
Viral Rna ◽  
Detection Methods ◽  
Scientific Methodology ◽  
Rna Modifications ◽  
Chemical Modifications ◽  
Viral Protein Synthesis ◽  
Antibody Assays ◽  
Rna Export ◽  
Generation Sequencing

ABSTRACT Chemical modifications of viral RNA are an integral part of the viral life cycle and are present in most classes of viruses. To date, more than 170 RNA modifications have been discovered in all types of cellular RNA. Only a few, however, have been found in viral RNA, and the function of most of these has yet to be elucidated. Those few we have discovered and whose functions we understand have a varied effect on each virus. They facilitate RNA export from the nucleus, aid in viral protein synthesis, recruit host enzymes, and even interact with the host immune machinery. The most common methods for their study are mass spectrometry and antibody assays linked to next-generation sequencing. However, given that the actual amount of modified RNA can be very small, it is important to pair meticulous scientific methodology with the appropriate detection methods and to interpret the results with a grain of salt. Once discovered, RNA modifications enhance our understanding of viruses and present a potential target in combating them. This review provides a summary of the currently known chemical modifications of viral RNA, the effects they have on viral machinery, and the methods used to detect them.

scholarly journals Hydroxysafflor Yellow A: A Systematical Review on Botanical Resources, Physicochemical Properties, Drug Delivery System, Pharmacokinetics, and Pharmacological Effects

2020 ◽  
Vol 11 ◽  
Author(s):  
Feng Zhao ◽  
Ping Wang ◽  
Yuanyuan Jiao ◽  
Xiaoxiao Zhang ◽  
Daquan Chen ◽  
...  
Keyword(s):  
Carthamus Tinctorius ◽  
Clinical Applications ◽  
Detection Methods ◽  
Pharmacological Effects ◽  
Hydroxysafflor Yellow A ◽  
Pharmacological Activities ◽  
Related Research ◽  
Chemical Instability ◽  
Anticancer Effects ◽  
Systematical Review

Hydroxysafflower yellow A (HSYA), as a principal natural ingredient extracted from safflower (Carthamus tinctorius L.), has significant pharmacological activities, such as antioxidant, anti-inflammatory, anticoagulant, and anticancer effects. However, chemical instability and low bioavailability have been severely hampering the clinical applications of HSYA during the treatment of cardiovascular and cerebrovascular disease. Therefore, this present review systematically summarized the materials about HSYA, including acquisition methods, extraction and detection methods, pharmacokinetics, pharmacological effects and molecular mechanism, especially focus on the possible causes and resolutions about the chemical instability and low bioavailability of HSYA, in order to provide relatively comprehensive basic data for the related research of HSYA.

scholarly journals Wiki-Gendersort: Automatic gender detection using first names in Wikipedia

2020 ◽  
Author(s):  
Nicolas Bérubé ◽  
Gita Ghiasi ◽  
Maxime Sainte-Marie ◽  
Vincent Larivière
Keyword(s):  
Web Of Science ◽  
Detection Algorithm ◽  
Detection Methods ◽  
Related Research ◽  
Gender Information ◽  
Research Questions ◽  
Gender Detection ◽  
The Web

Gender information is often absent from databases available to scholars, thus hindering the proper problematization, investigation, and answering of various gender-related research questions. Named-based algorithms represent the most simple, yet effective used gender detection methods: such methods proceed by generating first-name-to-gender mapping tables based on user records in a given dataset and then applying such mapping tables "in reversal" to other databases for completion or validation purposes. The present research aims to develop a gender detection algorithm focusing on the gender detection of eponymous Wikipedia pages and compare its performance to that of other well-known gender detection databases, using the author names indexed in the Web of Science.

Review of the distribution and detection methods of heavy metals in the environment

2020 ◽  
Vol 12 (48) ◽  
pp. 5747-5766
Author(s):  
Mengting Jin ◽  
Hao Yuan ◽  
Bo Liu ◽  
Jiajia Peng ◽  
Liping Xu ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Spatial Distribution ◽  
Research Trends ◽  
Detection Methods ◽  
Related Research

The spatial distribution of heavy metals, representative detection methods of heavy metals and related research trends are summarized.

The Booming Field of Epitranscriptomics and its Role in Human Disease

2021 ◽  
Author(s):  
Spring Libray
Keyword(s):  
Human Disease ◽  
Detection Methods ◽  
Sequencing Analysis ◽  
Chemical Modifications ◽  
Detection Techniques

Currently, the detection techniques used for transcriptome-wide identification of chemical modifications mainly depend on chemical and antibody-based detection methods followed by sequencing analysis.

scholarly journals Effects of mRNA Modifications on Translation: An Overview

2021 ◽  
pp. 327-356
Author(s):  
Bijoyita Roy
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Life Cycle ◽  
Mrna Translation ◽  
Detection Methods ◽  
Messenger Rnas ◽  
Biological Functions ◽  
Chemical Modifications ◽  
Potential Applications ◽  
Synthetic Mrna

AbstractThe mRNA epitranscriptome imparts diversity to gene expression by installing chemical modifications. Advances in detection methods have identified chemical modifications in eukaryotic, bacterial, and viral messenger RNAs (mRNAs). The biological functions of modifications in mRNAs still remain to be understood. Chemical modifications are introduced in synthetic mRNAs meant for therapeutic applications to maximize expression from the synthetic mRNAs and to evade the host immune response. This overview provides a background of chemical modifications found in mRNAs, with an emphasis on pseudouridine and its known effects on the mRNA life cycle, its potential applications in synthetic mRNA, and the methods used to assess its effects on mRNA translation.

Aspiration cytology and transmission electron microscopy (TEM): A novel tissue-collection technique with useful diagnostic applications

1984 ◽  
Vol 42 ◽  
pp. 90-93
Author(s):  
J.A. Maksem ◽  
C. VanDyke ◽  
H.W. Carter ◽  
C.F. Galang
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Diagnostic Technique ◽  
Needle Aspiration ◽  
Large Series ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Transmission Electron ◽  
Diagnostic Applications ◽  
Microscopic Slide

In the last decade fine needle aspiraration biopsy has gained recognition as a valuable diagnostic technique, and its benefits have been demonstrated in large series of patients with almost every type of tumor (1,2). The usual way to collect cellular material from needle-aspiration biopsies is to discharge the needle and syringe contents onto a microscopic slide and smear the material with another slide. The entire specimen is contained on the slides prepared at the time of biopsy. Serious technical difficulties are inherent to this method. 1) Inconsistent fixation, 2) drying artifact, 3) loss of tissue fragments, 4) inability to confirm impressions by a “second method”, and 5) retention of significant diagnostic material in the needle hub. Our technique avoids these difficulties.

DNA nick end labeling: a reliable marker for apoptosis?

1995 ◽  
Vol 53 ◽  
pp. 962-963
Author(s):  
Anne F. Bushnell ◽  
Sarah Webster ◽  
Lynn S. Perlmutter
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Apoptotic Cell ◽  
Morphological Characteristics ◽  
Detection Methods ◽  
Tissue Preparation ◽  
Preparation Methods ◽  
Dna Laddering ◽  
Disease States ◽  
Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

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