scholarly journals Non-Cavitation Targeted Microbubble-Mediated Single-Cell Sonoporation

Micromachines ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 113
Author(s):  
Xiufang Liu ◽  
Wenjun Zhang ◽  
Yanshu Jing ◽  
Shasha Yi ◽  
Umar Farooq ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Single Cell ◽  
Acoustic Radiation ◽  
Survival Rates ◽  
Radiation Force ◽  
Input Voltage ◽  
High Survival ◽  
Bubble Cavitation ◽  
Efficient Delivery ◽  
The Impact

Sonoporation employs ultrasound accompanied by microbubble (MB) cavitation to induce the reversible disruption of cell membranes and has been exploited as a promising intracellular macromolecular delivery strategy. Due to the damage to cells resulting from strong cavitation, it is difficult to balance efficient delivery and high survival rates. In this paper, a traveling surface acoustic wave (TSAW) device, consisting of a TSAW chip and a polydimethylsiloxane (PDMS) channel, was designed to explore single-cell sonoporation using targeted microbubbles (TMBs) in a non-cavitation regime. A TSAW was applied to precisely manipulate the movement of the TMBs attached to MDA-MB-231 cells, leading to sonoporation at a single-cell level. The impact of input voltage and the number of TMBs on cell sonoporation was investigated. In addition, the physical mechanisms of bubble cavitation or the acoustic radiation force (ARF) for cell sonoporation were analyzed. The TMBs excited by an ARF directly propelled cell membrane deformation, leading to reversible perforation in the cell membrane. When two TMBs adhered to the cell surface and the input voltage was 350 mVpp, the cell sonoporation efficiency went up to 83%.

scholarly journals Cognitive decline in patients with prostate cancer: study protocol of a prospective cohort, NEON-PC

BMJ Open ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. e043844
Author(s):  
Natalia Araujo ◽  
Samantha Morais ◽  
Ana Rute Costa ◽  
Raquel Braga ◽  
Ana Filipa Carneiro ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cognitive Decline ◽  
Cognitive Performance ◽  
Androgen Deprivation Therapy ◽  
Survival Rates ◽  
Cardiovascular Complications ◽  
Androgen Deprivation ◽  
High Survival ◽  
The Impact

IntroductionProstate cancer is the most prevalent oncological disease among men in industrialised countries. Despite the high survival rates, treatments are often associated with adverse effects, including metabolic and cardiovascular complications, sexual dysfunction and, to a lesser extent, cognitive decline. This study was primarily designed to evaluate the trajectories of cognitive performance in patients with prostate cancer, and to quantify the impact of the disease and its treatments on the occurrence of cognitive decline.MethodsParticipants will be recruited from two main hospitals providing care to approximately half of the patients with prostate cancer in Northern Portugal (Portuguese Institute of Oncology of Porto and São João Hospital Centre), and will comprise a cohort of recently diagnosed patients with prostate cancer proposed for different treatment plans, including: (1) radical prostatectomy; (2) brachytherapy and/or radiotherapy; (3) radiotherapy in combination with androgen deprivation therapy and (4) androgen deprivation therapy (with or without chemotherapy). Recruitment began in February 2018 and is expected to continue until the first semester of 2021. Follow-up evaluations will be conducted at 1, 3, 5, 7 and 10 years. Sociodemographic, behavioural and clinical characteristics, anxiety and depression, health literacy, health status, quality of life, and sleep quality will be assessed. Blood pressure and anthropometrics will be measured, and a fasting blood sample will be collected. Participants’ cognitive performance will be evaluated before treatments and throughout follow-up (Montreal Cognitive Assessment and Cube Test as well as Brain on Track for remote monitoring). All participants suspected of cognitive impairment will undergo neuropsychological tests and clinical observation by a neurologist.Ethics and disseminationThe study was approved by the Ethics Committee of the hospitals involved. All participants will provide written informed consent, and study procedures will be developed to ensure data protection and confidentiality. Results will be disseminated through publication in peer-reviewed journals and presentation in scientific meetings.

The Impact of Herbivores on Regeneration in Four Trees From Arid Australia.

1995 ◽  
Vol 17 (2) ◽  
pp. 213 ◽  
Author(s):  
TD Auld
Keyword(s):  
Survival Data ◽  
National Park ◽  
Survival Rates ◽  
Ground Level ◽  
High Survival ◽  
Grazing Land ◽  
The Impact ◽  
Acacia Ligulata ◽  
Semi Arid ◽  
Survival Of Seedlings

The size distributions of populations of four semi-arid perennial trees were investigated within Kinchega National Park in western NSW. For Acacia ligulata, A, loderi and Alectryon oleifolius, it appears that regeneration has been eliminated or at best severely limited at most sites. Some regeneration has occurred through recruitment of vegetative suckers in Casuarina pauper. Currently these vegetative recruits have high survival rates under both rabbit and kangaroo grazing, although such grazing frequently reduces the height of vegetative suckers back to ground level. Survival of seedlings of Acacia ligulata was very limited, with highest survival when mammals were excluded. There was no survival of seedlings of Casuarina pauper in the presence of rabbits and survival was poor when rabbits were excluded. Many seedlings of both species die through desiccation. The survival data from seedlings and vegetative suckers reinforce the patterns observed in the size distribution of populations. Within Kinchega National Park, control of rabbits is essential to initiate regeneration. A reduction in the total grazing pressure (especially rabbits, sheep, cattle and goats) is necessary in critical dry periods on semi-arid and arid grazing land if regeneration of perennials is to be encouraged.

scholarly journals Acoustic radiation force impulse accuracy and the impact of hepatic steatosis on liver fibrosis staging

2016 ◽  
Vol 60 (5) ◽  
pp. 587-592 ◽  
Author(s):  
Nicole Harris ◽  
David Nadebaum ◽  
Michael Christie ◽  
Alexandra Gorelik ◽  
Amanda Nicoll ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Hepatic Steatosis ◽  
Acoustic Radiation ◽  
Radiation Force ◽  
Acoustic Radiation Force ◽  
Acoustic Radiation Force Impulse ◽  
The Impact ◽  
Fibrosis Staging

scholarly journals Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach

2021 ◽  
Author(s):  
Sangnam Kim ◽  
Sunho Moon ◽  
Sunghoon Rho ◽  
Sangpil Yoon
Keyword(s):  
Single Cell ◽  
Acoustic Radiation ◽  
Ultrasonic Transducer ◽  
Radiation Force ◽  
Solid Sphere ◽  
Acoustic Radiation Force ◽  
Ultrasonic Transducers ◽  
Ultrahigh Frequency ◽  
Cell Phenotype ◽  
Target Cells

AbstractEven though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single cell level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure actual acoustic radiation force (ARF) applied to target cells. Therefore, we have developed an approach to measure ARF of ultrahigh frequency ultrasonic transducers using theoretical model of the dynamics of a solid sphere in a gelatin phantom. To estimate ARF at the focus of 130 MHz transducer, we matched measured maximum displacements of a solid sphere with theoretical calculations. We selected appropriate ranges of input voltages and pulse durations for single cell applications and estimated ARF were in the range of tens of pN to nN. FRET live cell imaging was demonstrated to visualize calcium transport between cells after a target single cell was stimulated by the developed ultrasonic transducer.

Application of a Geriatric Injury Protocol Demonstrates High Survival Rates for Geriatric Trauma Patients with High Injury Acuity

2017 ◽  
Vol 83 (10) ◽  
pp. 1122-1126
Author(s):  
Tigran Karamanukyan ◽  
Andrea Pakula ◽  
Maureen Martin ◽  
Ashwitha Francis ◽  
Ruby Skinner
Keyword(s):  
Injury Severity ◽  
Survival Rates ◽  
Geriatric Trauma ◽  
Study Cohort ◽  
High Survival ◽  
Trauma Patients ◽  
Advanced Directives ◽  
Severity Scores ◽  
The Mean ◽  
The Impact

Geriatric trauma has historically been associated with poor outcomes, particularly in the setting of severe polytrauma. Although geriatric trauma protocols are common, there are limited data on their impact in patients with high injury severity. In this study, we sought to investigate the impact of a geriatric injury protocol on outcomes in patients with severe trauma acuity. Ninety-eight geriatric patients (age ≥65) admitted to our trauma center with injury severity scores (ISS) ≥15 comprised the study cohort. The mean age was 75 ± 7.7 yrs. The mean ISS was 25 ± 9.2, and the mean geriatric trauma outcome score was 150 ± 3. Mortality was 17 per cent and 70 per cent were due to central nervous system injury. When patients with nonsurvivable injuries or advanced directives resulting in early care withdrawal were excluded, the mortality was 6 per cent. Extremes of age did not impact mortality [(>80 years, 21%) vs (65–79, 16%, P = 0.5)]. Most patients (53%) were discharged home. The application of our geriatric trauma protocol led to favorable results despite high injury acuity. These data suggest that even at the extremes of age, a large percentage of patients can be expected to survive. A prospective validation of these findings is warranted.

scholarly journals Acoustic radiation force impulse imaging: normal values of spleen stiffness in healthy children

2021 ◽  
Author(s):  
Sylviane Hanquinet ◽  
Céline Habre ◽  
Méryle Laurent ◽  
Mehrak Anooshiravani ◽  
Seema Toso
Keyword(s):  
Normal Values ◽  
Acoustic Radiation ◽  
Radiation Force ◽  
Acoustic Radiation Force ◽  
Acoustic Radiation Force Impulse ◽  
Healthy Children ◽  
Significant Difference ◽  
Spleen Stiffness ◽  
The Impact ◽  
Arfi Imaging

Abstract Background Acoustic radiation force impulse (ARFI) imaging is a noninvasive ultrasound elastography technique for evaluating tissue stiffness. The association of liver and spleen stiffness provides additional information in the assessment of portal hypertension. The technique and normal values of spleen stiffness by point shear wave elastography (p-SWE) in pediatrics have not been well documented. Objective Our aim is to describe the feasibility and normal ARFI elastography values in the spleen for healthy children and to compare measurements in two different probe positions (the axial and sagittal planes). Materials and methods Spleen p-SWE using ARFI values were measured with a 6C1 probe in 102 healthy children (age range: 8 weeks to 17 years) divided into four age groups. An average of nine (standard deviation: two) spleen stiffness measurements were taken during free breathing in each plane (axial and sagittal). The impact of age and measurement plane in the spleen was analyzed using multivariate models. Results There was no significant difference in spleen stiffness values taken at different ages, with an average of the medians of 2.43±0.31 m/s. There was no significant difference based on probe orientation: sagittal plane (median: 2.46±0.29 m/s) and axial plane (median: 2.43±0.32 m/s) with Student’s t-test P=0.18. The mean depth of measurement varied between 2.3 cm and 3.7 cm, according to age. Conclusion Normal spleen stiffness values using ARFI imaging in children do not vary with age and correspond to a median of 2.43 m/s. No significant difference was found when using different probe positions.

The Impact of Backboard Placement on Chest Compression Quality: A Mannequin Study

2019 ◽  
Vol 34 (02) ◽  
pp. 182-187 ◽  
Author(s):  
Erkman Sanri ◽  
Sinan Karacabey
Keyword(s):  
Chest Compression ◽  
Survival Rates ◽  
Control Group ◽  
High Survival ◽  
Undergraduate Medical Students ◽  
High Quality ◽  
Mean Values ◽  
The Difference ◽  
The Impact ◽  
Experimental Group

Introduction:High-quality chest compressions (CCs) are associated with high survival rates and good neurological outcomes in cardiac arrest patients. The 2015 American Heart Association (AHA; Dallas, Texas USA) Guidelines for Resuscitation defined and recommended high-quality CCs during cardiopulmonary resuscitation (CPR). However, CPR providers struggle to achieve high-quality CCs. There is a debate about the use of backboards during CPR in literature. Some studies suggest backboards improve CC quality, whereas others suggest that backboards can cause delays. This is the first study to evaluate all three components of high-quality CCs: compression depth, recoil depth, and rate, at the same time with a high number of subjects. This study evaluated the impact of backboards on CC quality during CPR. The primary outcome was the difference in successful CC rates between two groups.Methods:This was a randomized, controlled, single-blinded study using a high-fidelity mannequin. The successful CC rates, means CC depths, recoil depths, and rates achieved by 6th-grade undergraduate medical students during two minutes of CPR were compared between two randomized groups: an experimental group (backboard present) and a control group (no backboard).Results:Fifty-one of all 101 subjects (50.5%) were female, and the mean age was 23.9 (SD = 1.01) years. The number and the proportion of successful CCs were significantly higher in the experimental group (34; 66.7%) when compared to the control group (19; 38.0%; P = .0041). The difference in mean values of CC depth, recoil depth, and CC rate was significantly higher in the experiment group.Conclusion:The results suggest that using a backboard during CPR improves the quality of CCs in accordance with the 2015 AHA Guidelines.Sanri E, Karacabey S. The impact of backboard placement on chest compression quality: a mannequin study. Prehosp Disaster Med. 2019;34(2):182–187

Rationale Abklärung von Lebererkrankungen

2017 ◽  
Vol 74 (3) ◽  
pp. 79-86
Author(s):  
Leona von Köckritz ◽  
Andrea De Gottardi
Keyword(s):  
Acoustic Radiation ◽  
Radiation Force ◽  
Acoustic Radiation Force ◽  
Acoustic Radiation Force Impulse ◽  
Alkalische Phosphatase ◽  
Gamma Glutamyltransferase ◽  
Alpha 1 Antitrypsin

Zusammenfassung. Im klinischen Alltag werden häufig erhöhte Leberwerte beobachtet. In der Regel erfordern sie weitere Abklärungen bezüglich der möglichen Ätiologie und des Schweregrad einer akuten oder chronischen Lebererkrankung. Die Abklärung sollte dabei neben einer gezielten Anamnese und sorgfältigen klinischen Untersuchung, auch die Bestimmung von laborchemischen Markern für Cholestase und Leberfunktionsstörungen (wie Alkalische Phosphatase, gamma-Glutamyltransferase, Bilirubin, Albumin und Gerinnungsfaktoren) umfassen. Die Bestimmung weiterer Parameter wie Ferritin und Transferrinsättigung, Autoimmunantikörper, Virusserologien, alpha-1 Antitrypsin und Coerulolasmin können weitere Hinweise für die kausalen Zusammenhänge der Leberfunktionsstörung liefern. Bei Patienten mit Lebererkrankungen ist eine sonografische Beurteilung der Leber obligat. Ergänzend zur Sonografie werden heute weitere nicht-invasive Methoden wie Fibroscan, Acoustic Radiation Force Impulse Elastometrie und Magnet-Resonanz-Elastografie zur Beurteilung der Leberfibrose eingesetzt. In ausgewählten Fällen ist eine Leberbiopsie notwendig, um den Grad der Fibrose und die Ätiologie der Lebererkrankung zu eruieren. Mithilfe eines Fallbeispiels, diskutieren die Autoren im Folgenden die rationale Anwendung diagnostischer Tests und deren korrekte Interpretation und schlagen eine Orientierungshilfe zur rationalen Abklärung von Patienten mit Lebererkrankungen vor.

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