scholarly journals Chemical Ecology of Streptomyces albidoflavus Strain A10 Associated with Carpenter Ant Camponotus vagus

2020 ◽  
Vol 8 (12) ◽  
pp. 1948
Author(s):  
Anna A. Baranova ◽  
Alexey A. Chistov ◽  
Anton P. Tyurin ◽  
Igor A. Prokhorenko ◽  
Vladimir A. Korshun ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Antagonistic Activity ◽  
Ecological Communities ◽  
Crucial Importance ◽  
Carpenter Ants ◽  
Streptomyces Albidoflavus ◽  
Defensive Symbiosis ◽  
Carpenter Ant ◽  
Actinomycete Strain ◽  
Small Hydrophobic

Antibiotics produced by symbiotic microorganisms were previously shown to be of crucial importance for ecological communities, including ants. Previous works on ant–actinobacteria symbiosis are mainly focused on farming ants, which use antifungal microbial secondary metabolites to control pathogens in their fungal gardens. In this work, we studied microorganisms associated with carpenter ant Camponotus vagus. Pronounced antifungal activity of isolated actinobacteria strain A10 was found to be facilitated by biosynthesis of the antimycin A complex, consisting of small hydrophobic depsipeptides with high antimicrobial and cytotoxic activity. The actinomycete strain A10 was identified as Streptomyces albidoflavus. We studied the antagonistic activity of strain A10 against several entomopathogenic microorganisms. The antifungal activity of this strain potentially indicates a defensive symbiosis with the host ant, producing antimycins to protect carpenter ants against infections. The nature of this ant-microbe association however remains to be established.

scholarly journals Antifungal activity of several isolates of Trichoderma against Cladosporium and Botrytis

2018 ◽  
Vol 8 (1) ◽  
pp. 88-91 ◽  
Author(s):  
M. Skaptsov ◽  
S. Smirnov ◽  
M. Kutsev ◽  
O. Uvarova ◽  
T. Sinitsyna ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Its Region ◽  
Botanical Garden ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Morphological Observation ◽  
The South

<p><em>Trichoderma</em> isolates (SSBGT07, SSBGT08, SSBGT09, SSBGT10) were isolated from the soil samples of the South-Siberian Botanical Garden and identified using morphological observation and ITS region analysis as <em>Trichoderma harzianum</em>, <em>T. asperellum, T. ghanense</em>, and <em>T. longibranchiatum</em>. Antagonistic activity against <em>Cladosporium </em>sp. and<em> Botrytis </em>sp. was evaluated <em>in vitro</em>. All isolates showed antagonistic effect by competition against <em>Cladosporium </em>sp. <em>T. asperellum </em>and <em>T. longibranchiatum</em> showed antagonism against <em>Botrytis </em>sp. All isolates showed hyper sporulation on the sclerotia of <em>Botrytis</em> sp. (except the <em>T. ghanense</em>) and colonies of the <em>Cladosporium</em> sp. Our study provides new isolates that affect the <em>Cladosporium </em>sp. and<em> Botrytis </em>sp.</p>

scholarly journals In vitro and in vivo toxicity of fungicides and biofungicides for the control of verticillium and fusarium wilt of pepper

2021 ◽  
Vol 36 (1) ◽  
pp. 23-34
Author(s):  
Milica Mihajlovic ◽  
Emil Rekanovic ◽  
Jovana Hrustic ◽  
Mila Grahovac ◽  
Brankica Tanovic
Keyword(s):  
Antifungal Activity ◽  
Verticillium Dahliae ◽  
Antagonistic Activity ◽  
In Vitro Assays ◽  
Thiophanate Methyl ◽  
Significant Difference ◽  
By Products ◽  
Pepper Plants

A survey of in vitro and in vivo sensitivity of Verticillium dahliae and Fusarium oxysporum to several commercial fungicides and biofungicides was undertaken. In in vitro assays, the tested isolate of V. dahliae proved to be very sensitive to difenoconazole (EC50 = 0.02 mg/l). However, under greenhouse conditions, the highest efficacy in V. dahliae control on inoculated pepper plants was recorded for a product based on thiophanate-methyl (83.10% compared to control). Among the tested fungicides, the lowest efficacy was recorded for a product based on azoxystrobin (23.10 %) with no significant difference compared to control (p > 0.05). In in vitro assays, the tested F. oxysporum isolate was the most sensitive to prochloraz (EC50 = 0.07 mg/l) and the least sensitive to fluopyram (EC50 = 1075.01 mg/l). In in vivo assay, the highest efficacy was achieved by products based on captan (95.60%), and the lowest by a product based on thiophanate-methyl (54.40%). Antagonistic activity of the bacterium B. subtilis under laboratory conditions was not satisfying. Also, the antifungal activity and spectrum of a tested product based on tee tree oil was not efficient in suppressing pepper wilting caused by V. dahliae and F. oxysporum.

scholarly journals Antifungal activity of indigenous Bacillus spp. isolated from soil

2017 ◽  
pp. 261-269 ◽  
Author(s):  
Dragana Bjelic ◽  
Maja Ignjatov ◽  
Jelena Marinkovic ◽  
Nemanja Spremo ◽  
Maja Karaman ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Verticillioides ◽  
Fungal Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Fungal Species ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Plant Diseases ◽  
Bacterial Isolates ◽  
Bacillus Spp

Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.

scholarly journals The Predatory Myxobacterium Citreicoccus inhibens gen. nov. sp. nov. Showed Antifungal Activity and Bacteriolytic Property against Phytopathogens

2021 ◽  
Vol 9 (10) ◽  
pp. 2137
Author(s):  
Yang Zhou ◽  
Shuoxing Yi ◽  
Yi Zang ◽  
Qing Yao ◽  
Honghui Zhu
Keyword(s):  
Antifungal Activity ◽  
Pathogenic Bacteria ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Extracellular Proteins ◽  
Morphological Observation ◽  
Rrna Gene ◽  
Fruiting Body Formation ◽  
Predatory Bacteria ◽  
Beneficial Traits

The application and promotion of biological control agents are limited because of poor efficacy and unstable performance in the field. Screening microorganisms with high antagonistic activity, effective adaptability, and high field-survival should be prospected. Myxobacteria are soil predatory bacteria with wide adaptability, which are considered as good antagonists. Here, we report a myxobacterium strain M34 isolated from subtropical forest soil in South China using the Escherichia coli baiting method. Based on the morphological observation, physiological test, biochemical characteristics, 16S rRNA gene sequence, and genomic data, strain M34 was identified as a novel genus and novel species, representing a new clade of Myxococcaceae, for which the name Citreicoccus inhibens gen. nov. sp. nov. is proposed (type strain M34T = GDMCC 1.2275T = KCTC 82453T). The typical features of M34, including fruiting body formation and extracellular fibrillar interconnection, indicated by microscopic observations, contributed to cell adaption in different environments. Furthermore, the strain showed antifungal activity against phytopathogenic fungi and predatory activity to both Gram-negative and Gram-positive phytopathogenic bacteria. The bioprotective mechanisms are attributed to the presence of pyrrolnitrin and derivative with antifungal activity and the extracellular proteins with lytic activity against pathogenic bacteria. Due to its multiple beneficial traits, strain M34 has the potential to be developed into a versatile biocontrol agent for the management of both fungal and bacterial phytopathogens.

scholarly journals Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean

2019 ◽  
Vol 7 (8) ◽  
pp. 243 ◽  
Author(s):  
Chongxi Liu ◽  
Xiaoxin Zhuang ◽  
Zhiyin Yu ◽  
Zhiyan Wang ◽  
Yongjiang Wang ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
High Throughput Sequencing ◽  
Antagonistic Activity ◽  
Diseased Plant ◽  
Natural Defense ◽  
Independent Analysis ◽  
Culture Independent ◽  
Potential Reservoir ◽  
Endophytic Actinobacteria

The present study was conducted to examine the influence of a pathogen Sclerotinia sclerotiorum (Lib.) de Bary on the actinobacterial community associated with the soybean roots. A total of 70 endophytic actinobacteria were isolated from the surface-sterilized roots of either healthy or diseased soybeans, and they were distributed under 14 genera. Some rare genera, including Rhodococcus, Kribbella, Glycomyces, Saccharothrix, Streptosporangium and Cellulosimicrobium, were endemic to the diseased samples, and the actinobacterial community was more diverse in the diseased samples compared with that in the heathy samples. Culture-independent analysis of root-associated actinobacterial community using the high-throughput sequencing approach also showed similar results. Four Streptomyces strains that were significantly abundant in the diseased samples exhibited strong antagonistic activity with the inhibition percentage of 54.1–87.6%. A bioactivity-guided approach was then employed to isolate and determine the chemical identity of antifungal constituents derived from the four strains. One new maremycin analogue, together with eight known compounds, were detected. All compounds showed significantly antifungal activity against S. sclerotiorum with the 50% inhibition (EC50) values of 49.14–0.21 mg/L. The higher actinobacterial diversity and more antifungal strains associated with roots of diseased plants indicate a possible role of the root-associated actinobacteria in natural defense against phytopathogens. Furthermore, these results also suggest that the root of diseased plant may be a potential reservoir of actinobacteria producing new agroactive compounds.

scholarly journals Space, Habitat and Isolation are the Key Determinants of Tree Colonization by the Carpenter Ant in Plantation Forests

Forests ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 630 ◽  
Author(s):  
Adam Véle ◽  
Jakub Horák
Keyword(s):  
Norway Spruce ◽  
Ad Hoc ◽  
Spatial Scales ◽  
Brown Rot ◽  
Forest Plantations ◽  
Ant Nests ◽  
Real Nature ◽  
Carpenter Ants ◽  
Carpenter Ant ◽  
Coniferous Plantation

Forest plantations are still often considered the antithesis of real nature. However, plantations can host many organisms. The problem is that some of the hosted species are regarded ad hoc as pests. The main aim of our paper was to study the carpenter ant (Camponotus ligniperdus) in windstorm habitats. We studied forests in East Bohemia, Czech Republic, and focused on the spatial distribution of snapped trees and the influence of selected forest characteristics on the incidence of ant nests. We found that the nests in the study area mainly occurred in Norway spruce, which is the most commercially important tree in the majority of Central Europe. More than one quarter of the snapped trees were inhabited by the ants. We found that nests exhibited a spatially autocorrelated pattern that differed on spatial scales. The most important characteristic of the host tree for determining carpenter ant nests was the presence of brown rot, and the majority of tree nests were isolated from forest openings. The presence of carpenter ants in forest plantations is, therefore, not harmful. Their presence could be used by forest managers as an indication of unsuitable stand conditions for the successful growth of the Norway spruce and other coniferous plantation trees.

The Biology of Carpenter Ants in New Brunswick

1964 ◽  
Vol 96 (6) ◽  
pp. 894-909 ◽  
Author(s):  
C. J. Sanders
Keyword(s):  
Life Cycle ◽  
New Brunswick ◽  
Forest Floor ◽  
Wood Chips ◽  
Balsam Fir ◽  
Carpenter Ants ◽  
Carpenter Ant ◽  
Eastern White Cedar ◽  
Cycle Covers ◽  
Frequency Curves

AbstractThirty-five colonies of three species of carpenter ant were examined: 25 of Camponotus herculeanus, 9 of C. noveboracensis, and 1 of C. pennsylvanicus. Workings were examined in about 150 trees. No major differences were noticed in the biology of the first two species.All individuals were taken from two overwintering, torpid, C. herculeanus colonies; the larger contained 6,000 workers, 550 winged forms, and 6,500 larvae. Larvae were of two sizes and it is thought that the life cycle covers two years in New Brunswick. Frequency curves of worker head widths show that there are majors and minors, the minors being more numerous, especially in small colonies.Each colony typically occupied several trees, only one of which contained the brood; the others had less extensive workings. Entrances were underground and led to tunnels connecting the trees within each colony. Surplus wood chips were buried in the ground. Ant activity in the tunnels was continuous through the day and night; few ants were seen on the forest floor.Woodpeckers are important enemies and had attacked one third of the brood trees.The workings frequently assist wind breakage and damage butt logs. From the roots they extend to a height of 4-6 ft., occasionally higher. 1% of the spruce, and 2-4% of the balsam fir examined had been attacked. This may lead to loss of 10% of merchantable volume, and possibly a higher figure for eastern white cedar.

scholarly journals Changes in antagonistic activity of lactic acid bacteria induced by their response to technological factors

2015 ◽  
Vol 24 (4) ◽  
Author(s):  
Dovile Jonkuviene ◽  
Joana Šalomskienė ◽  
Asta Abraitienė ◽  
Irena Mačionienė ◽  
Jūratė Repečkienė ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Antifungal Activity ◽  
Lactic Acid Bacteria ◽  
Food Production ◽  
Negative Impact ◽  
Antagonistic Activity ◽  
Diffusion Method ◽  
High Antimicrobial Activity ◽  
Technological Factors

This study examined the changes in antagonistic activity of selected lactic acid bacteria (LAB) in response to technological factors used in food production. The antimicrobial activity of 12 selected LAB strains was assessed against the bacterial and fungal strains using the agar well diffusion method. Sodium chloride (NaCl) 6.5–8.0%, glucose 20-30% and pH 4.0, pH 8.0 and higher were the most crucial factors in reducing the spectra of the microorganisms antagonized. Heating at 80 °C or 100 °C had a greater negative impact than 63 °C on the antifungal activity of LAB. Freezing at –72 °C eliminated the antifungal activity, or it changed from fungicidal to fungistatic. Although each LAB demonstrated the ability to retain antimicrobial activity induced by various technological factors, Lactococcus lactis 768/5 was superior in retaining high antimicrobial activity against tested indicator strains.

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