scholarly journals In vitro and in vivo toxicity of fungicides and biofungicides for the control of verticillium and fusarium wilt of pepper

2021 ◽  
Vol 36 (1) ◽  
pp. 23-34
Author(s):  
Milica Mihajlovic ◽  
Emil Rekanovic ◽  
Jovana Hrustic ◽  
Mila Grahovac ◽  
Brankica Tanovic
Keyword(s):  
Antifungal Activity ◽  
Verticillium Dahliae ◽  
Antagonistic Activity ◽  
In Vitro Assays ◽  
Thiophanate Methyl ◽  
Significant Difference ◽  
By Products ◽  
Pepper Plants

A survey of in vitro and in vivo sensitivity of Verticillium dahliae and Fusarium oxysporum to several commercial fungicides and biofungicides was undertaken. In in vitro assays, the tested isolate of V. dahliae proved to be very sensitive to difenoconazole (EC50 = 0.02 mg/l). However, under greenhouse conditions, the highest efficacy in V. dahliae control on inoculated pepper plants was recorded for a product based on thiophanate-methyl (83.10% compared to control). Among the tested fungicides, the lowest efficacy was recorded for a product based on azoxystrobin (23.10 %) with no significant difference compared to control (p > 0.05). In in vitro assays, the tested F. oxysporum isolate was the most sensitive to prochloraz (EC50 = 0.07 mg/l) and the least sensitive to fluopyram (EC50 = 1075.01 mg/l). In in vivo assay, the highest efficacy was achieved by products based on captan (95.60%), and the lowest by a product based on thiophanate-methyl (54.40%). Antagonistic activity of the bacterium B. subtilis under laboratory conditions was not satisfying. Also, the antifungal activity and spectrum of a tested product based on tee tree oil was not efficient in suppressing pepper wilting caused by V. dahliae and F. oxysporum.

scholarly journals Biocontrol potential of Trichoderma harzianum in controlling wilt disease of pistachio caused by Verticillium dahliae

2017 ◽  
Vol 57 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Zeinab Fotoohiyan ◽  
Saeed Rezaee ◽  
Gholam Hosein Shahidi Bonjar ◽  
Amir Hossein Mohammadi ◽  
Mohammad Moradi
Keyword(s):  
Verticillium Dahliae ◽  
Trichoderma Harzianum ◽  
Pathogenic Fungus ◽  
Antagonistic Activity ◽  
Wilt Disease ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Biocontrol Potential

Abstract Verticillium wilt caused by Verticillium dahliae, is one of the most devastating diseases in pistachio orchards in the world including Iran. In search for an effective non-chemical strategy for the management of this disease, we evaluated the biocontrol potential of Trichoderma harzianum isolates obtained from the rhizosphere of healthy pistachio trees in different locations of the Kerman province of Iran against V. dahliae under laboratory and greenhouse conditions. Dual culture tests in the laboratory were conducted in a completely randomized design using 72 T. harzianum isolates. Twenty isolates showed the highest in vitro antagonistic activity. The results indicated that all 20 isolates were capable of inhibiting the mycelial growth of V. dahliae significantly. Among them, isolates Tr8 and Tr19 were the most effective by 88.89% and 85.12% inhibition, respectively. Extracted cell free metabolites of all effective isolates also inhibited the growth of V. dahliae in the culture medium significantly. According to the results, isolates Tr4 and Tr6 inhibited fungal pathogen growth by 94.94% and 88.15% respectively, through production of non-volatile metabolites. In the evaluation of volatile metabolites, isolates Tr5 and Tr4 were the most effective by 26.27% and 24.49% growth inhibition, respectively. Based on the results of the in vitro experiments, the five most effective isolates were selected for evaluation under greenhouse conditions for their biocontrol potential in controlling Verticillium wilt of pistachio. Results of the greenhouse, (in vivo) experiments were positive and indicated that the occurrence of wilt disease in plants treated with the antagonists alone or in combination with pathogenic fungus was lower than in plants inoculated with pathogen alone. The overall results of this study suggest that Trichoderma fungal antagonist may be an effective biocontrol agent for the control of Verticillium wilt of pistachio.

scholarly journals Phytochemical composition, antifungal activity, in vitro and in vivo toxicity of Syzygium cumini (L.) Skeels leaves extract

2021 ◽  
Vol 20 (5) ◽  
pp. 536-555
Author(s):  
Ernani Canuto Figueiredo Junior ◽  
◽  
Yuri Wanderley Cavalcanti ◽  
Andressa Brito Lira ◽  
Hilzeth de Luna Freire Pessoa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Galleria Mellonella ◽  
In Vitro Assays ◽  
Syzygium Cumini ◽  
Candida Spp ◽  
In Vivo Toxicity ◽  
Phytochemical Composition

This study determined phytochemical composition, antifungal activity and toxicity in vitro and in vivo of Syzygium cumini leaves extract (Sc). Thus, was characterized by gas chromatography coupled to mass spectrometry and submitted to determination of Minimum Inhibitory (MIC) and Fungicidal concentrations (MFC) on reference and clinical strains of Candida spp. and by growth kinetics assays. Toxicity was verified using in vitro assays of hemolysis, osmotic fragility, oxidant and antioxidant activity in human erythrocytes and by in vivo acute systemic toxicity in Galleria mellonella larvae. Fourteen different compounds were identified in Sc, which showed antifungal activity (MIC between 31.25-125 μg/mL) with fungistatic effect on Candida. At antifungal concentrations, it demonstrated low cytotoxicity, antioxidant activity and neglible in vivo toxicity. Thus, Sc demonstrated a promising antifungal potential, with low toxicity, indicating that this extract can be a safe and effective alternative antifungal agent.

scholarly journals Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 286 ◽  
Author(s):  
Van Anh Ngo ◽  
San-Lang Wang ◽  
Van Bon Nguyen ◽  
Chien Thang Doan ◽  
Thi Ngoc Tran ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Endophytic Bacteria ◽  
Black Pepper ◽  
Antagonistic Activity ◽  
Piper Nigrum ◽  
Control Group ◽  
Vitro Assay ◽  
16S Rna

In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the antifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings treated with the promising bacteria had the lowest rate of root disease (8.45–11.21%) and lower fatal rate (11.11–15.55%) compared to the control group (24.81% and 24.44%). In addition, the three promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.

scholarly journals Effects of fungicides and biofungicides on Rhizoctonia solani, a pathogen of pepper

2020 ◽  
Vol 35 (2) ◽  
pp. 97-104
Author(s):  
Milica Mihajlovic ◽  
Emil Rekanovic ◽  
Jovana Hrustic ◽  
Mila Grahovac ◽  
Marija Stevanovic ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Tea Tree Oil ◽  
Thiophanate Methyl ◽  
Tea Tree ◽  
Pepper Plants

In vitro and in vivo sensitivity of Rhizoctonia solani to several commercial fungicides and biofungicides was studied. An isolate of R. solani, derived from diseased pepper plants originating from a greenhouse in Knjazevac, Serbia, was used. The highest efficacy in R. solani control under greenhouse conditions was achieved by iprodione (95.80%, compared to control), although differences in the effectiveness of iprodione, tea tree oil, azoxystrobin and thiophanate-methyl were not statistically significant. The isolate was sensitive to all tested products in vitro. The obtained EC50s were: 0.43 mg/l for iprodione, 1.84 mg/l for thiophanate-methyl, 13.84 mg/l for prochloraz, 430.37 mg/l for fluopyram, 596.60 mg/l for azoxystrobin, and 496.79 mg/l for tea tree oil.

scholarly journals Analysis of Virulence of Clinical Isolates ofSalmonella enteritidis In Vivo and In Vitro

1999 ◽  
Vol 67 (11) ◽  
pp. 5651-5657 ◽  
Author(s):  
Sangwei Lu ◽  
Amee R. Manges ◽  
Yisheng Xu ◽  
Ferric C. Fang ◽  
Lee W. Riley
Keyword(s):  
Clinical Isolates ◽  
In Vitro Assays ◽  
Complex Nature ◽  
Phage Types ◽  
Food Borne ◽  
Oral Inoculation ◽  
Wide Range ◽  
Significant Difference

ABSTRACT Salmonella enterica serotype Enteritidis (S. enteritidis) is a major food-borne pathogen, and its incidence among all Salmonella serotypes has increased dramatically in the last two decades. To study the virulence characteristics of clinical isolates of S. enteritidis, we determined the 50% lethal doses (LD50) in mice of isolates of two major phage types (4 and 8). Isolates of both phage types showed a wide range of LD50 after oral inoculation, varying from under 102 organisms to over 108 organisms. No significant difference in LD50 was observed between the phage types. These observations indicated that clinical isolates ofS. enteritidis are highly heterogeneous in their ability to cause death in mice. We compared the LD50s of these isolates to the results observed from in vitro pathogenicity assays. We also analyzed these isolates for recognized Salmonellavirulence loci (spv, sodCI, sopE, and sef). The in vitro phenotypes of the isolates showed no obvious correlation with their LD50 in any given assay, and the virulence genes tested were present in all isolates. However, the isolate with the lowest LD50 (isolate 97A 2472) was resistant to acidified sodium nitrite (ASN). Moreover, the most acid-susceptible, macrophage-susceptible, and ASN-susceptible isolates were attenuated for virulence in mice. These results, based on extensive analysis of clinical isolates of S. enteritidis, demonstrate the complex nature of Salmonella pathogenesis in mice. Our results also indicate the limitation of in vitro assays in predicting in vivo virulence.

scholarly journals Compost Tea Induces Growth and Resistance against Rhizoctonia solani and Phytophthora capsici in Pepper

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 781
Author(s):  
Ana Isabel González-Hernández ◽  
M. Belén Suárez-Fernández ◽  
Rodrigo Pérez-Sánchez ◽  
María Ángeles Gómez-Sánchez ◽  
María Remedios Morales-Corts
Keyword(s):  
Plant Growth ◽  
Rhizoctonia Solani ◽  
Growth Parameters ◽  
Phytophthora Capsici ◽  
Nutrient Content ◽  
In Vitro Assays ◽  
Open Flower ◽  
Pepper Plants

Compost teas (CTs) are organic solutions that constitute an interesting option for sustainable agriculture. Those that come from garden waste have been applied in vitro and in vivo on pepper plants to determine its suppressive effect against both Phytophthora capsici and Rhizoctonia solani. The studied CT showed relevant content in NO3−, K2O, humic acids, and microorganisms such as aerobic bacteria, N-fixing bacteria, and actinobacteria, which play a role in plant growth and resistance. This rich abundance of microbiota in the CT induced a reduction in the relative growth rate of both P. capsici and R. solani (31.7% and 38.0%, respectively) in in vitro assays compared to control. In addition, CT-irrigated plants displayed increased growth parameters and showed the first open flower one week before those treatments without CTs, which suggests that its application advanced the crop cycle. Concerning pathogen infection, damage caused by both pathogens became more apparent with a one-week inoculation compared to a four-week inoculation, which may indicate that a microbiological and chemical balance had been reached to cope with biotic stresses. Based on these results, we conclude that CT application induces plant growth and defense in pepper plants against P. capsici and R. solani because of its relevant soluble nutrient content and microbiota richness, which provides a novel point for plant nutrition and protection in horticultural crops.

A in Vivo Assay for Standardizing Heparin Preparations

1979 ◽  
Vol 41 (03) ◽  
pp. 576-582
Author(s):  
A R Pomeroy
Keyword(s):  
In Vitro Assays ◽  
British Pharmacopoeia ◽  
In Vitro Method ◽  
Standard Preparation ◽  
Reliable Estimation ◽  
Assay Procedure ◽  
Accuracy And Precision ◽  
Heparin Preparation

SummaryThe limitations of currently used in vitro assays of heparin have demonstrated the need for an in vivo method suitable for routine use.The in vivo method which is described in this paper uses, for each heparin preparation, four groups of five mice which are injected intravenously with heparin according to a “2 and 2 dose assay” procedure. The method is relatively rapid, requiring 3 to 4 hours to test five heparin preparations against a standard preparation of heparin. Levels of accuracy and precision acceptable for the requirements of the British Pharmacopoeia are obtained by combining the results of 3 to 4 assays of a heparin preparation.The similarity of results obtained the in vivo method and the in vitro method of the British Pharmacopoeia for heparin preparations of lung and mucosal origin validates this in vivo method and, conversely, demonstrates that the in vitro method of the British Pharmacopoeia gives a reliable estimation of the in vivo activity of heparin.

Potentially Thrombogenic Materials in Factor IX Concentrates

1975 ◽  
Vol 33 (03) ◽  
pp. 617-631 ◽  
Author(s):  
H. S Kingdon ◽  
R. L Lundblad ◽  
J. J Veltkamp ◽  
D. L Aronson
Keyword(s):  
Human Plasma ◽  
Antithrombin Iii ◽  
Factor Ix ◽  
In Vitro Assays ◽  
Substantial Agreement ◽  
Coefficient Of Correlation

SummaryFactor IX concentrates manufactured from human plasma and intended for therapeutic infusion in man have been suspected for some time of being potentially thrombogenic. In the current studies, assays were carried out in vitro and in vivo for potentially thrombogenic materials. It was possible to rank the various materials tested according to the amount of thrombogenic material detected. For concentrates not containing heparin, there was substantial agreement between the in vivo and in vitro assays, with a coefficient of correlation of 0.77. There was no correlation between the assays for thrombogenicity and the antithrombin III content. We conclude that many presently available concentrates of Factor IX contain substantial amounts of potentially thrombogenic enzymes, and that this fact must be considered in arriving at the decision whether or not to use them therapeutically.

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

In-silico Studies of Isolated Phytoalkaloid Against Lipoxygenase: Study Based on Possible Correlation

2018 ◽  
Vol 21 (3) ◽  
pp. 215-221
Author(s):  
Haroon Khan ◽  
Muhammad Zafar ◽  
Helena Den-Haan ◽  
Horacio Perez-Sanchez ◽  
Mohammad Amjad Kamal
Keyword(s):  
In Silico ◽  
Docking Studies ◽  
In Vivo Studies ◽  
In Vitro Assays ◽  
Chronic Obstructive ◽  
Lipoxygenase Inhibitors ◽  
Lipoxygenase Inhibition ◽  
In Silico Studies

Aim and Objective: Lipoxygenase (LOX) enzymes play an important role in the pathophysiology of several inflammatory and allergic diseases including bronchial asthma, allergic rhinitis, atopic dermatitis, allergic conjunctivitis, rheumatoid arthritis and chronic obstructive pulmonary disease. Inhibitors of the LOX are believed to be an ideal approach in the treatment of diseases caused by its over-expression. In this regard, several synthetic and natural agents are under investigation worldwide. Alkaloids are the most thoroughly investigated class of natural compounds with outstanding past in clinically useful drugs. In this article, we have discussed various alkaloids of plant origin that have already shown lipoxygenase inhibition in-vitro with possible correlation in in silico studies. Materials and Methods: Molecular docking studies were performed using MOE (Molecular Operating Environment) software. Among the ten reported LOX alkaloids inhibitors, derived from plant, compounds 4, 2, 3 and 1 showed excellent docking scores and receptor sensitivity. Result and Conclusion: These compounds already exhibited in vitro lipoxygenase inhibition and the MOE results strongly correlated with the experimental results. On the basis of these in vitro assays and computer aided results, we suggest that these compounds need further detail in vivo studies and clinical trial for the discovery of new more effective and safe lipoxygenase inhibitors. In conclusion, these results might be useful in the design of new and potential lipoxygenase (LOX) inhibitors.

Sign in / Sign up

Export Citation Format

Share Document