scholarly journals Asbestos Fibers and Ferruginous Bodies Detected by VP-SEM/EDS in Colon Tissues of a Patient Affected by Colon-Rectum Cancer: A Case Study

Minerals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 658
Author(s):  
Caterina Rinaudo ◽  
Alessandro Croce ◽  
Stefania Erra ◽  
Elisabetta Nada ◽  
Marinella Bertolotti ◽  
...  
Keyword(s):  
Chemical Characterization ◽  
Variable Pressure ◽  
Neoplastic Tissue ◽  
Colon Tissue ◽  
Fresh Tissue ◽  
Tissue Samples ◽  
Asbestos Fibers ◽  
History Of ◽  
Healthy Area ◽  
Long Fibers

The aim of this work was to inspect the presence of asbestos fibers in colon tissue from a patient, with history of indirect exposure to asbestos and affected by colon cancer, who underwent surgery. Variable pressure scanning electron microscopy, coupled with energy dispersive spectroscopy (VP-SEM/EDS), was used for identification of inorganic fibers and for their morphological- chemical characterization. Fresh tissue samples from both, healthy area close to the neoplasia and from the neoplastic regions, were separately digested to eliminate the biological matrix. The precipitate was analyzed by VP-SEM/EDS, identifying in samples from healthy tissue asbestos bodies and small asbestos fibers, and in samples from neoplastic tissue long fibers of asbestos, free from covering. A quantification of the asbestos bodies and the free fibers in the two type of specimens is proposed. Moreover, to locate the fibers in the biological medium, histological sections from the colon of the same patient were also examined. Free asbestos fibers appeared concentrated in the tissue bridge between the healthy and the neoplastic areas. Immuno-histochemical investigation performed on the neoplasia seems to exclude a role of microsatellite instability in the carcinogenesis process, suggesting an influence of the fibers.

Asbestos fiber identification in liver from cholangiocarcinoma patients living in an asbestos polluted area: a preliminary study

2019 ◽  
Vol 105 (5) ◽  
pp. 404-410 ◽  
Author(s):  
Federica Grosso ◽  
Alessandro Croce ◽  
Roberta Libener ◽  
Narciso Mariani ◽  
Massimo Pastormerlo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Asbestos Exposure ◽  
Optical Microscope ◽  
Variable Pressure ◽  
Chrysotile Asbestos ◽  
Neoplastic Tissue ◽  
Asbestos Fibers ◽  
Preliminary Study ◽  
Scanning Electron

Purpose: To assess whether asbestos fibers may be observed in liver tissue of patients with cholangiocarcinoma (CC) with environmental or working asbestos exposure. Methods: Detection of fibers was performed directly on histologic sections of liver from 7 patients with CC using optical microscope and variable pressure scanning electron microscopy equipped with energy-dispersive spectroscopy (VP-SEM/EDS). All patients were from Casale Monferrato, Italy, a highly asbestos-polluted town. Due to ethical constraints, observers were blinded to patients’ clinical features. Results: Fibers/bundles of fibers of chrysotile were detected in 5 out of 7 patients (71%). The boundary between healthy and neoplastic tissue or the fibrocollagen tissue produced by the neoplasia were identified as areas of fiber incorporation. Conclusions: This study is the first report about the detection of chrysotile asbestos fibers in the liver of patients with CC. Further studies on larger cohorts are needed to corroborate our preliminary findings.

scholarly journals Phylogeny of Thripophagini ovenbirds (Aves: Synallaxinae: Furnariidae)

2019 ◽  
Vol 127 (4) ◽  
pp. 826-846
Author(s):  
Esther Quintero ◽  
Utku Perktaş
Keyword(s):  
Taxonomic Revision ◽  
Mitochondrial Genes ◽  
Evolutionary Relationships ◽  
Diverse Group ◽  
Fresh Tissue ◽  
Tissue Samples ◽  
Climatic Oscillations ◽  
The Andes ◽  
History Of ◽  
Time Of Divergence

Abstract In this study, we address the evolutionary relationships and discuss the biogeographical history of a complex and diverse group of ovenbirds, the Thripophagini. We reconstruct the phylogeny and estimate the time of divergence of this group, using sequences from two complete mitochondrial genes (cytochrome b and NADH subunit 2) from a total of 115 fresh tissue samples. The results provide a better understanding of the phylogenetic relationships of the taxa within this group, some of which require a thorough taxonomic revision. We discuss the biogeographical history of the group, and find parallels with other previously studied Andean birds which may indicate that tectonic and climatic events might, at least in part, be linked to its diversification through the uplift of the Andes, the creation of new montane habitats and barriers, the evolution of Amazonian drainages and landscapes, and the climatic oscillations of the Pleistocene.

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

scholarly journals Reactivation of BK Polyomavirus in Urine Cytology Is Not Associated with Urothelial Cell Carcinoma

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1412
Author(s):  
Faisal Klufah ◽  
Ghalib Mobaraki ◽  
Axel zur Hausen ◽  
Iryna V. Samarska
Keyword(s):  
Cell Carcinoma ◽  
Urine Cytology ◽  
Urothelial Cell ◽  
The Other ◽  
Urothelial Cell Carcinoma ◽  
Tissue Samples ◽  
Bk Polyomavirus ◽  
Ffpe Tissues ◽  
Immunosuppressed Patients ◽  
History Of

BK polyomavirus (BKPyV) has been associated with some high-grade and special urothelial cell carcinoma (UCC) subtypes in immunosuppressed patients. Here, we evaluated the relationship of BKPyV-positive urine cytology specimens (UCS) with UCC. A large single-institution database was retrospectively searched for UCS positive for decoy cells, suggesting BKPyV infection. These were tested for the presence of BKPyV by PCR and immunohistochemistry (IHC) in urine sediments and formalin-fixed paraffin-embedded (FFPE) tissue samples of UCC. Decoy cells were reported in 30 patients out of the database with 22.867 UCS. Of these 30 patients, 16 (53.3%) had no history of UCC. Six patients out of these 16 had a history of transplantation, 4 had a history of severe chronic medical conditions, and 6 had no chronic disease. The other fourteen patients were diagnosed with either in situ or invasive UCC of the urinary bladder (14/30; 46.6%) prior to the detection of decoy cells in the urine. Nine of these UCC patients received intravesical treatment (BCG or mitomycin) after the first presentation with UCC. However, the clinical data on the treatment of the other five UCC patients was lacking. IHC identified BKPyV-positivity in the urine samples of non-UCC and UCC patients, while no BKPyV positivity was found in FFPE tissues of primary UCCs and metastases. In addition, BKPyV-PCR results revealed the presence of BKPyV DNA in the urine of the UCC cases, yet none in the UCC tissues itself. These data strongly indicate that BKPyV reactivation is not restricted to immunosuppression. It can be found in UCS of the immunocompetent patients and may be related to the intravesical BCG or mitomycin treatment of the UCC patients.

scholarly journals Identification of Mycobacterium genavense natural infection in a domestic ferret

2018 ◽  
Vol 31 (1) ◽  
pp. 133-136 ◽  
Author(s):  
Bérengère Dequéant ◽  
Quentin Pascal ◽  
Héloïse Bilbault ◽  
Elie Dagher ◽  
Maria-Laura Boschiroli ◽  
...  
Keyword(s):  
Natural Infection ◽  
Abdominal Ultrasound ◽  
Needle Aspiration ◽  
Mesenteric Lymph Nodes ◽  
Mustela Putorius ◽  
Tissue Samples ◽  
Acid Fast Bacilli ◽  
Mycobacterium Genavense ◽  
History Of ◽  
Progressive Weight Loss

A 6-y-old neutered male ferret ( Mustela putorius furo) was presented because of a 1-mo history of progressive weight loss, chronic cough, and hair loss. On clinical examination, the animal was coughing, slightly depressed, moderately hypothermic, and had bilateral epiphora. Thoracic radiography was suggestive of severe multinodular interstitial pneumonia. Abdominal ultrasound examination revealed hepatosplenomegaly and mesenteric and pancreaticoduodenal lymphadenopathy. Fine-needle aspiration of the pancreaticoduodenal lymph node, followed by routine Romanowsky and Ziehl–Neelsen stains, revealed numerous macrophages containing myriad acid-fast bacilli, leading to identification of mycobacteriosis. Autopsy and histologic examination confirmed the presence of disseminated, poorly defined, acid-fast, bacilli-rich granulomas in the pancreaticoduodenal and mesenteric lymph nodes, intestines, and lungs. Destaining of May-Grünwald/Giemsa–stained slides with alcohol, and then restaining with Ziehl–Neelsen, revealed acid-fast rods and avoided repeat tissue sampling without affecting the Ziehl–Neelsen stain quality and cytologic features. Tissue samples were submitted for a PCR assay targeting the heat shock protein gene ( hsp65) and revealed 100% homology with Mycobacterium genavense. We emphasize the use of special stains and PCR for identification of this potential zoonotic agent.

scholarly journals An Untypical Case of Gouty Infiltration of Both Peroneal Tendons and a Longitudinal Lesion of the Peroneus Brevis Tendon Mimicking Synovial Sarcoma

2018 ◽  
Vol 2018 ◽  
pp. 1-3 ◽  
Author(s):  
Konstantinos Anagnostakos ◽  
Andreas Thiery ◽  
Christof Meyer ◽  
Octavian Tapos
Keyword(s):  
Synovial Sarcoma ◽  
Histopathological Examination ◽  
Soft Tissue Tumors ◽  
Resonance Imaging ◽  
Open Biopsy ◽  
Tissue Samples ◽  
Peroneal Tendons ◽  
Orthopedic Surgeons ◽  
History Of ◽  
Peroneus Brevis

We present a case of a 70-year-old male patient with an untypical gout infiltration of the peroneal tendons mimicking synovial sarcoma. The patient had a negative history of gout at initial presentation in our department. Magnetic resonance imaging of the region revealed a finding highly suspicious for synovial sarcoma of the peroneal tendons. Open biopsy was performed. Histopathological examination of the tissue samples demonstrated the presence of gout with no signs of malignancy. The gout infiltration was excised in a subsequent surgery. Orthopedic surgeons should be aware of the potential manifestation of gout in tendons and bear this in mind in the differential diagnosis of soft tissue tumors.

Validation of proton stopping power ratio estimation based on dual energy CT using fresh tissue samples

2017 ◽  
Vol 63 (1) ◽  
pp. 015012 ◽  
Author(s):  
Vicki T Taasti ◽  
Gregory J Michalak ◽  
David C Hansen ◽  
Amanda J Deisher ◽  
Jon J Kruse ◽  
...  
Keyword(s):  
Dual Energy ◽  
Stopping Power ◽  
Dual Energy Ct ◽  
Power Ratio ◽  
Ratio Estimation ◽  
Fresh Tissue ◽  
Tissue Samples

scholarly journals Dichromatobolus, a new genus of spirobolidan millipedes from Madagascar (Spirobolida, Pachybolidae)

2020 ◽  
Vol 720 ◽  
pp. 107-120
Author(s):  
Thomas Wesener
Keyword(s):  
New Genus ◽  
Color Pattern ◽  
The Body ◽  
Scanning Electron Micrographs ◽  
Fresh Tissue ◽  
Pet Trade ◽  
Tissue Samples ◽  
Molecular Barcoding ◽  
Line Drawings ◽  
Loose Soil

A new genus, Dichromatobolus gen. nov., belonging to the genus-rich mainly southern hemisphere family Pachybolidae of the order Spirobolida, is described based on D. elephantulus gen. et sp. nov., illustrated with color pictures, line drawings, and scanning electron micrographs. The species is recorded from the spiny bush of southwestern Madagascar. Dichromatobolus elephantulus gen. et sp. nov. shows an unusual color pattern, sexual dichromatism with males being red with black legs and females being grey. Males seem to be more surface active, as mainly males were collected with pitfall traps. Females mainly come from the pet trade. The body of this species is short and very wide, being only 8 times longer than wide in the males. Live observations show the species is a very slow mover, digging in loose soil almost as fast as walking on the surface. The posterior gonopods of Dichromatobolus gen. nov. are unusually simple and well-rounded, displaying some similarities to the genera Corallobolus Wesener, 2009 and Granitobolus Wesener, 2009, from which the new genus differs in numerous other characters, e.g., size, anterior gonopods and habitus. Despite several attempts with fresh tissue samples and different primers, molecular barcoding did not work for Dichromatobolus gen. nov. Any relationships to the other 15 genera of Pachybolidae indigenous to Madagascar remain unknown.

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