scholarly journals Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells

Molecules ◽  
2015 ◽  
Vol 20 (4) ◽  
pp. 6573-6591 ◽  
Author(s):  
Dayanne Gomes ◽  
Cinthia Telles ◽  
Mariana Costa ◽  
Jailma Almeida-Lima ◽  
Leandro Costa ◽  
...  
Keyword(s):  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
Brown Seaweeds ◽  
Dictyota Menstrualis ◽  
Methanolic Extracts

New Hybrid Scaffolds Based on Carbazole-Chalcones as Potent Anticancer Agents

2020 ◽  
Vol 20 ◽  
Author(s):  
Faisal Rashid ◽  
Sumera Zaib ◽  
Aliya Ibrar ◽  
Syeda Abida Ejaz ◽  
Aamer Saeed ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
G1 Arrest ◽  
Human Breast ◽  
Caspase 9 ◽  
Flow Cytometric ◽  
Pharmacodynamic Profile ◽  
Microscopic Studies ◽  
Mcf 7

Background and Objectives: Despite various technological advances for the treatment of cancer, the identification of new chemical entities with potent anticancer effects remain an indispensable requirement of the time due to multi-drug resistance exhibited by previously developed anticancer drugs. Particularly, the hybrid drugs incorporating two individual bioactive pharmacophores present medicinally important structural leads, thus improving the pharmacodynamic profile of the drug molecules. The antiproliferative and pro-apoptotic activity of the carbazole-chalcone hybrids on human breast and cervical cancer cells will be examined. Materials and Methods: To overcome such complications, in the current study, we evaluated the cytotoxic effects of carbazole-chalcone hybrids on human breast adenocarcinoma (MCF-7), cervical adenocarcinoma (HeLa) cells and normal cells i.e., baby hamster kidney cells (BHK-21) using MTT (dimethyl-2-thiazolyl-2,5-diphenyl-2H-tetrazolium bromide) assay. The mechanistic studies were performed on potent compound 4g by fluorescent microscopic studies, release of lactate dehydrogenase (LDH) and mitochondrial membrane potential, activation of caspase-9 and -3 and flow cytometric analysis. Results: As revealed by MTT assay, compound 4g was identified as the most potent derivative among the tested series with IC50 values of 5.64 and 29.15 μM against HeLa and MCF-7 cells, respectively. The results were compared with cisplatin. Fluorescent microscopic studies using 4′,6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) staining confirmed the occurrence of apoptosis in HeLa cells treated with the most active compound 4g. Moreover, compound 4g also triggered the release of lactate dehydrogenase (LDH) in treated HeLa and MCF-7 cells while a luminescence assay displayed a remarkable increase in the activity of caspase-9 and -3. Moreover, flow cytometric results revealed that compound 4g caused G0/G1 arrest in the treated HeLa cells. Conclusion: Our results demonstrated that the compound 4g possesses chemotherapeutic properties against breast cancer and cervical adenocarcinoma cells, thus warranting further research to test the anticancer efficacy of this compound at clinical level.

scholarly journals Extracts from Pulsatilla patens target cancer-related signaling pathways in HeLa cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Grażyna Łaska ◽  
Magdalena Maciejewska-Turska ◽  
Elwira Sieniawska ◽  
Łukasz Świątek ◽  
David S. Pasco ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Hela Cells ◽  
Luciferase Reporter ◽  
Cervical Cancer Cell Line ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Triterpenoid Saponins ◽  
Cancer Signaling ◽  
Methanolic Extracts ◽  
Pulsatilla Patens

AbstractThe purpose of this study was to determine if a methanolic extract of the Pulsatilla patens (L.) Mill. can inhibit the progression of cancer through the modulation of cancer-related metabolic signaling pathways. We analyzed a panel of 13 inducible luciferase reporter gene vectors which expression is driven by enhancer elements that bind to specific transcription factors for the evaluation of the activity of cancer signaling pathways. The root extract of P. patens exhibited strong inhibition of several signaling pathways in HeLa cells, a cervical cancer cell line, and was found to be the most potent in inhibiting the activation of Stat3, Smad, AP-1, NF-κB, MYC, Ets, Wnt and Hdghog, at a concentration of 40 µg/mL. The methanolic extracts of P. patens enhanced apoptotic death, deregulated cellular proliferation, differentiation, and progression towards the neoplastic phenotype by altering key signaling molecules required for cell cycle progression. This is the first study to report the influence of Pulsatilla species on cancer signaling pathways. Further, our detailed phytochemical analysis of the methanolic extracts of the P. patens allowed to deduce that compounds, which strongly suppressed the growth and proliferation of HeLa cancer cells were mainly triterpenoid saponins accompanied by phenolic acids.

scholarly journals The in vitro effects of compound-X on growth, morphology, the induction of autophagy and apoptosis, as well as cell cycle progression in a cervical adenocarcinoma cell line

2012 ◽  
Vol 31 (1) ◽  
Author(s):  
S. Marais ◽  
T.V. Mqoco ◽  
B.A. Stander ◽  
R. Prudent ◽  
L. Lafanechère ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Hela Cells ◽  
Cell Cycle Progression ◽  
Cervical Adenocarcinoma ◽  
Growth Morphology ◽  
Adenocarcinoma Cell Line ◽  
Cycle Progression ◽  
Adenocarcinoma Cell

It can be concluded that compound-X induced both autophagy and apoptosis as a means of celldeath in HeLa cells.

scholarly journals Crinane Alkaloids of the Amaryllidaceae with Cytotoxic Effects in Human Cervical Adenocarcinoma (HeLa) Cells

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Jerald J. Nair ◽  
Lucie Rárová ◽  
Miroslav Strnad ◽  
Jaume Bastida ◽  
Lee Cheesman ◽  
...  
Keyword(s):  
South Africa ◽  
Indigenous Peoples ◽  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
Cytotoxic Activities ◽  
Adenocarcinoma Cell Line ◽  
Cytotoxic Effects ◽  
Adenocarcinoma Cell ◽  
The Family ◽  
History Of

The family Amaryllidaceae has a long history of usage in the traditional medicinal practices of the indigenous peoples of South Africa, with three of its species known to be used for cancer treatment. Furthermore, the Amaryllidaceae is widely recognized for its unique alkaloid constituents, several of which exhibit potent and selective cytotoxic activities. In this study, several crinane alkaloids derived from local Amaryllidaceae species were examined for cytotoxic effects against the human cervical adenocarcinoma cell line, of which distichamine was the most potent (IC50 2.2 μM).

On the mechanism of phenotypic conversion of human cervical adenocarcinoma HeLa cells surviving infection by influenza B virus: Potential implications for biological management of adenocarcinomas

2008 ◽  
Vol 204 (5) ◽  
pp. 329-334 ◽  
Author(s):  
Despina O. Sioutopoulou ◽  
Elias Plakokefalos ◽  
Leonidas Arvanitis ◽  
Evangelos Athanassiou ◽  
John Venizelos ◽  
...  
Keyword(s):  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
Influenza B Virus ◽  
Influenza B ◽  
B Virus ◽  
Biological Management

scholarly journals EVALUATION OF THE ANTICANCER PROPERTIES OF TURMESAC® IN HUMAN CERVICAL ADENOCARCINOMA

2020 ◽  
pp. 109-113
Author(s):  
FIROZ HM ◽  
NANJUNDAIAH S ◽  
SADASHIVA CT
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
Anticancer Agent ◽  
Cervical Adenocarcinoma ◽  
Apoptosis Induction ◽  
Anticancer Properties ◽  
Cycle Arrest ◽  
Diphenyltetrazolium Bromide ◽  
Ic50 Value

Objective: In the current study, an extract of turmeric rhizome (Turmesac®) was evaluated for possible anticancer activity in human cervical adenocarcinoma (HeLa) cells. Methods: Turmesac®’s ability to elicit cytotoxicity in cancer cells was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (MTT) assay, where the IC50 value was determined. Apoptosis induction and cell cycle arrest were analyzed by flow cytometry post-Turmesac® IC50 value treatment for 24 h. Results: The determined IC50 value of Turmesac® in HeLa cells was 115.12 μg/ml. This concentration was able to induce apoptosis 2 times greater than the apoptotic standard, camptothecin, treated cells. Cell cycle arrest was observed at the G0/G1 and S phases in Turmesac® treated HeLa cells. Conclusion: Turmesac® shows the potential of being a promising anticancer agent that may be incorporated into chemotherapies, but further study is required to elucidate the exact mechanisms involved with longer treatment duration, as would be the case in clinical trial phases.

scholarly journals Viscum albumL. Extracts Protects HeLa Cells against Nuclear and Mitochondrial DNA Damage

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Evren Önay-Uçar ◽  
Özlem Erol ◽  
Başak Kandemir ◽  
Elif Mertoğlu ◽  
Ali Karagöz ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Hela Cells ◽  
Oxidative Dna Damage ◽  
Nuclear Dna ◽  
Viscum Album ◽  
Mitochondrial Dna Damage ◽  
Methanolic Extracts ◽  
Host Trees ◽  
Lime Tree

Viscum albumL. is a semiparasitic plant grown on trees and widely used for the treatment of many diseases in traditional and complementary therapy. It is well known that some activities ofViscum albumextracts are varied depending on the host trees, such as antioxidant, apoptosis-inducing, anticancer activities of the plant. The aim of the present study is to examine the comparative effects of methanolic extracts ofV. albumgrown on three different host trees (locust tree, lime tree, and hedge maple tree) on H2O2-induced DNA damage in HeLa cells. Oxidative damage in mitochondrial DNA and two nuclear regions was assessed by QPCR assay. The cells were pretreated with methanolic extracts (10 μg/mL) for 48 h, followed by the treatment with 750 μM H2O2for 1 hour. DNA damage was significantly induced by H2O2while it was inhibited byV. albumextracts. All extracts completely protected against nuclear DNA damage. While the extract from lime tree or white locust tree entirely inhibited mitochondrial DNA damage, that from hedge maple tree inhibited by only 50%. These results suggest that methanolic extracts ofV. albumcan prevent oxidative DNA damage, and the activity is dependent on the host tree.

scholarly journals Seminal Plasma Promotes the Expression of Tumorigenic and Angiogenic Genes in Cervical Adenocarcinoma Cells via the E-Series Prostanoid 4 Receptor

Endocrinology ◽  
2006 ◽  
Vol 147 (7) ◽  
pp. 3356-3365 ◽  
Author(s):  
Melissa Muller ◽  
Kurt J. Sales ◽  
Arieh A. Katz ◽  
Henry N. Jabbour
Keyword(s):  
Growth Factor ◽  
Seminal Plasma ◽  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
Dominant Negative Mutant ◽  
Mapk Kinase ◽  
Ep4 Receptor ◽  
Adenocarcinoma Cells ◽  
Cox 2 ◽  
Angiogenic Genes

E-series prostanoid (EP)4 receptor is up-regulated in numerous cancers, including cervical carcinomas, and has been implicated in mediating the effects of prostaglandin (PG)E2 in tumorigenesis. In addition to regulation by endogenously biosynthesized PGE2, neoplastic cervical epithelial cells in sexually active women may also be regulated by PGs present in seminal plasma. In this study, we investigated the signal transduction pathways mediating the role of seminal plasma and PGE2 in the regulation of tumorigenic and angiogenic genes via the EP4 receptor in cervical adenocarcinoma (HeLa) cells. HeLa cells were stably transfected with EP4 receptor in the sense orientation. Seminal plasma and PGE2 signaling via the EP4 receptor induced the activation of cyclooxygenase (COX)-2 and vascular endothelial growth factor (VEGF) promoters, expression of COX-2 and VEGF mRNA and protein, and secretion of VEGF protein into the culture medium. Treatment of HeLa cells with seminal plasma or PGE2 also rapidly induced the phosphorylation of ERK1/2 via the EP4 receptor. Preincubation of cells with a specific EP4 receptor antagonist (ONO-AE2-227) or chemical inhibitors of epidermal growth factor receptor (EGFR) tyrosine kinase or MAPK kinase or cotransfection of cells with dominant-negative mutant cDNA targeted against the EGFR, serine/threonine kinase Raf, or MAPK kinase abolished the EP4-induced activation of COX-2, VEGF, and ERK1/2. Therefore, we have demonstrated that seminal plasma and PGE2 can promote the expression of tumorigenic and angiogenic factors, in cervical adenocarcinoma cells via the EP4 receptor, EGFR, and ERK1/2 signaling pathways.

Sign in / Sign up

Export Citation Format

Share Document