scholarly journals Metabolomics Research Reveals the Mechanism of Action of Astragalus Polysaccharide in Rats with Digestive System Disorders

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3333 ◽  
Author(s):  
Huanjun Wang ◽  
Ana Liu ◽  
Wenxiao Zhao ◽  
Haijun Zhao ◽  
Lili Gong ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Amino Acid ◽  
Metabolic Pathways ◽  
Digestive System ◽  
Weight Bearing ◽  
High Fat ◽  
Low Protein ◽  
Astragalus Polysaccharide ◽  
Serum Metabolomics ◽  
Metabolomics Analysis

With the diversity of modern dietary lifestyles, digestive system disorders (DSD) have become a frequently occurring disease in recent years. Astragalus polysaccharide (APS) is a homogeneous polysaccharide extracted from Astragalus, which might ameliorate the digestive and absorptive functions. However, the treatment mechanisms remain unclear. In this study, rats with DSD were fed a high-fat–low-protein diet and subjected to weight-bearing swimming until exhaustion. When body weight and autonomous activities of the rats decreased, they were administered APS. After two weeks, serum metabolomics analysis based on LC-MS was performed to validate the therapeutic effect of APS and explore its mechanism. APS pharmacodynamics was determined in this study, and serum metabolomics analysis discovered and identified 16 significant, differentially produced metabolites involved in energy, amino acid, and lipid metabolism, including citric acid, lactic acid, alanine, phosphatidylcholine, phenylalanine. After treatment with APS, the levels of the above small-molecule metabolites were reversed. Our results show the efficacy of APS in DSD treatment through the regulation of perturbed metabolic pathways related to energy, amino acid, and lipid metabolism.

Serum metabolomics analysis reveals impaired lipid metabolism in rats after oral exposure to benzo(a)pyrene

2015 ◽  
Vol 11 (3) ◽  
pp. 753-759 ◽  
Author(s):  
Xiaoxue Wang ◽  
Jie Zhang ◽  
Qingyu Huang ◽  
Ambreen Alamdar ◽  
Meiping Tian ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Metabolic Profiling ◽  
Oral Exposure ◽  
Metabolomics Study ◽  
Serum Metabolomics ◽  
Metabolomics Analysis

A metabolomics study was conducted to unveil the metabolic profiling of rats exposed to benzo(a)pyrene, and twelve differentiated metabolites were identified.

High fat diet incorporated with meat proteins changes biomarkers of lipid metabolism, antioxidant activities, and the serum metabolomic profile in Glrx1−/− mice

2020 ◽  
Vol 11 (1) ◽  
pp. 236-252 ◽  
Author(s):  
Muhammad Ijaz Ahmad ◽  
Muhammad Umair Ijaz ◽  
Muzahir Hussain ◽  
Iftikhar Ali Khan ◽  
Noreen Mehmood ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Linoleic Acid ◽  
Bile Acid ◽  
Amino Acid ◽  
High Fat Diet ◽  
Antioxidant Activities ◽  
Glutathione Metabolism ◽  
Metabolomic Profile ◽  
High Fat ◽  
Meat Proteins

High-fat mutton protein diet may alter lipid-, linoleic acid-, amino acid-, bile acid-, sphingolipid-, glycine-, serine- and glutathione-metabolism pathways in Glrx−/− mice whereas HFF diet ameliorated NAFLD by modifying these pathways.

scholarly journals Differential and Synergistic Effects of Low Birth Weight and Western Diet on Skeletal Muscle Vasculature, Mitochondrial Lipid Metabolism and Insulin Signaling in Male Guinea Pigs

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4315
Author(s):  
Kristyn Dunlop ◽  
Ousseynou Sarr ◽  
Nicole Stachura ◽  
Lin Zhao ◽  
Karen Nygard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Birth Weight ◽  
Amino Acid ◽  
Low Birth Weight ◽  
Insulin Signaling ◽  
Western Diet ◽  
High Fat ◽  
High Sugar ◽  
Mitochondrial Lipid

Low birth weight (LBW) offspring are at increased risk for developing insulin resistance, a key precursor in metabolic syndrome and type 2 diabetes mellitus. Altered skeletal muscle vasculature, extracellular matrix, amino acid and mitochondrial lipid metabolism, and insulin signaling are implicated in this pathogenesis. Using uteroplacental insufficiency (UPI) to induce intrauterine growth restriction (IUGR) and LBW in the guinea pig, we investigated the relationship between UPI-induced IUGR/LBW and later life skeletal muscle arteriole density, fibrosis, amino acid and mitochondrial lipid metabolism, markers of insulin signaling and glucose uptake, and how a postnatal high-fat, high-sugar “Western” diet (WD) modulates these changes. Muscle of 145-day-old male LBW glucose-tolerant offspring displayed diminished vessel density and altered acylcarnitine levels. Disrupted muscle insulin signaling despite maintained whole-body glucose homeostasis also occurred in both LBW and WD-fed male “lean” offspring. Additionally, postnatal WD unmasked LBW-induced impairment of mitochondrial lipid metabolism, as reflected by increased acylcarnitine accumulation. This study provides evidence that early markers of skeletal muscle metabolic dysfunction appear to be influenced by the in utero environment and interact with a high-fat/high-sugar postnatal environment to exacerbate altered mitochondrial lipid metabolism, promoting mitochondrial overload.

Rapid increase in fibroblast growth factor 21 in protein malnutrition and its impact on growth and lipid metabolism

2015 ◽  
Vol 114 (9) ◽  
pp. 1410-1418 ◽  
Author(s):  
Yori Ozaki ◽  
Kenji Saito ◽  
Kyoko Nakazawa ◽  
Morichika Konishi ◽  
Nobuyuki Itoh ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Amino Acid ◽  
Growth Factor ◽  
Hepatic Steatosis ◽  
Growth Retardation ◽  
Protein Malnutrition ◽  
Fibroblast Growth Factor 21 ◽  
Fibroblast Growth ◽  
Low Protein ◽  
Igf I

AbstractProtein malnutrition promotes hepatic steatosis, decreases insulin-like growth factor (IGF)-I production and retards growth. To identify new molecules involved in such changes, we conducted DNA microarray analysis on liver samples from rats fed an isoenergetic low-protein diet for 8 h. We identified the fibroblast growth factor 21 gene (Fgf21) as one of the most strongly up-regulated genes under conditions of acute protein malnutrition (P<0·05, false-discovery rate<0·001). In addition, amino acid deprivation increased Fgf21 mRNA levels in rat liver-derived RL-34 cells (P<0·01). These results suggested that amino acid limitation directly increases Fgf21 expression. FGF21 is a polypeptide hormone that regulates glucose and lipid metabolism. FGF21 also promotes a growth hormone-resistance state and suppresses IGF-I in transgenic mice. Therefore, to determine further whether Fgf21 up-regulation causes hepatic steatosis and growth retardation after IGF-I decrease in protein malnutrition, we fed an isoenergetic low-protein diet to Fgf21-knockout (KO) mice. Fgf21-KO did not rescue growth retardation and reduced plasma IGF-I concentration in these mice. Fgf21-KO mice showed greater epididymal white adipose tissue weight and increased hepatic TAG and cholesterol levels under protein malnutrition conditions (P<0·05). Overall, the results showed that protein deprivation directly increased Fgf21 expression. However, growth retardation and decreased IGF-I were not mediated by increased FGF21 expression in protein malnutrition. Furthermore, FGF21 up-regulation rather appears to have a protective effect against obesity and hepatic steatosis in protein-malnourished animals.

scholarly journals 1H-NMR Metabolomics Analysis of the Effect of Rubusoside on Serum Metabolites of Golden Hamsters on a High-Fat Diet

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1274
Author(s):  
Li Li ◽  
Manjing Jiang ◽  
Yaohua Li ◽  
Jian Su ◽  
Li Li ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Amino Acid ◽  
1H Nmr ◽  
High Fat Diet ◽  
Ketone Bodies ◽  
High Fat ◽  
Nmr Metabolomics ◽  
Metabolism Regulation ◽  
Golden Hamsters ◽  
Lipid Metabolism Disorders

Rubusoside is a natural sweetener and the active component of Rubus suavissimus. The preventive and therapeutic effect of rubusoside on high-fat diet-induced (HFD) serum metabolite changes in golden hamsters was analyzed by 1H-NMR metabolomics to explore the underlying mechanism of lipid metabolism regulation. 1H-NMR serum metabolomics analyses revealed a disturbed amino acid-, sugar-, fat-, and energy metabolism in HFD animals. Animals supplemented with rubusoside can partly reverse the metabolism disorders induced by high-fat diet and exerted good anti-hypertriglyceridemia effect by intervening in some major metabolic pathways, involving amino acid metabolism, synthesis of ketone bodies, as well as choline and 4-hydroxyphenylacetate metabolism. This study indicates that rubusoside can interfere with and normalize high-fat diet-induced metabolic changes in serum and could provide a theoretical basis to establish rubusoside as a potentially therapeutic tool able to revert or prevent lipid metabolism disorders.

scholarly journals Effect of High Fat-Low Protein or Low Fat-High Protein Diet on Lipid Metabolism in Growing Chicks

1979 ◽  
Vol 50 (3) ◽  
pp. 138-141
Author(s):  
Keiichi TANAKA ◽  
Kazushige KITAHARA ◽  
Kakichi SHIGENO
Keyword(s):  
Lipid Metabolism ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
High Fat ◽  
Low Fat ◽  
Low Protein

scholarly journals Differential Metabolites in Chinese Autistic Children: A Multi-Centre Study Based on Urinary 1H-NMR Metabolomics Analysis

2020 ◽  
Author(s):  
Yu Ma ◽  
Hao Zhou ◽  
Chunpei Li ◽  
Xiaobing Zou ◽  
Xuerong Luo ◽  
...  
Keyword(s):  
Autism Spectrum Disorder ◽  
Amino Acid ◽  
Roc Curve ◽  
Metabolic Pathways ◽  
Autism Spectrum ◽  
Healthy Children ◽  
Autistic Children ◽  
Multi Centre Study ◽  
Research Centres ◽  
Metabolomics Analysis

Abstract Background: Autism spectrum disorder (ASD) is a group of early-onset neurodevelopmental disorders. However, there is no valuable biomarker for the early diagnosis of ASD. Our large-scale and multi-centre study aims to identify metabolic variations between ASD and healthy children and to investigate differential metabolites and associated pathogenic mechanisms.Methods: 117 autistic children and 119 healthy children were recruited from research centres of 7 cities. Urine samples were assayed by 1H-NMR metabolomics analysis to detect metabolic variations. Multivariate statistical analysis, including principal component analysis (PCA) and orthogonal projection to latent structure discriminant analysis (OPLS-DA), as well as univariate analysis were used to assess differential metabolites between the ASD and control groups. The differential metabolites were further analysed by receiver operating characteristics (ROC) curve analysis and metabolic pathways analysis.Results: Compared with the control group, the ASD group showed higher levels of glycine, guanidinoacetic acid, creatine, hydroxyphenylacetylglycine, phenylacetylglycine and formate and lower levels of 3-aminoisobutanoic acid, alanine, taurine, creatinine, hypoxanthine and N-methylnicotinamide. ROC curve showed relatively significant diagnostic values for hypoxanthine (area under the curve (AUC) = 0.657, 95% CI 0.588 to 0.726), creatinine (AUC = 0.639, 95% CI 0.569 to 0.709), creatine (AUC = 0.623, 95% CI 0.552 to 0.694), N-methylnicotinamide (AUC = 0.595, 95% CI 0.523 to 0.668) and guanidinoacetic acid (AUC = 0.574, 95% CI 0.501 to 0.647) in the ASD group. Combining the metabolites creatine, creatinine and hypoxanthine, the AUC of the ROC curve reached 0.720 (95% CI 0.659 to 0.777). Significantly altered metabolite pathways associated with differential metabolites were glycine, serine and threonine metabolism, arginine and proline metabolism, and taurine and hypotaurine metabolism.Limitations: Due to the restriction of research centres, inconsistent urine collection times may have affected the quality of metabolic analyses. Moreover, it is necessary to compare metabolite levels that vary with ASD severity to better clarify the pathogenesis of ASD.Conclusions: Urinary amino acid metabolites significantly altered in children with ASD. Amino acid metabolic pathways might play important roles in the pathogenic mechanisms of ASD.Clinical Trial registration: National Study on Autism Spectrum Disorder in China (NCT02200679). Registered July 24, 2014. https://www.clinicaltrials.gov/ct2/show/NCT02200679

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