scholarly journals Quality Evaluation of Tricholoma matsutake based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 34 ◽  
Author(s):  
Ying Xue ◽  
Wei Jin ◽  
Xian-Shun Xu ◽  
Li Yong ◽  
Bin Hu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Quality Evaluation ◽  
Southwest China ◽  
Total Content ◽  
Tricholoma Matsutake ◽  
Molecular Formula ◽  
Combined Use ◽  
Tof Ms

So far, there has been no quality evaluation of Tricholoma matsutake. Nucleic acid compounds are a kind of functional ingredient in T. matsutake that is beneficial to human health. In this study, a UPLC-TOF/MS method was first used to scan and identify the potential nucleic acid compounds in T. matsutake. Based on the calculation of the molecular formula and subsequent confirmation by authentic standards, 15 nucleic acid compounds were unambiguously identified: adenosine, cytidine, guanosine, inosine, thymidine, uridine, xanthosine dehydrate, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine, 2′-deoxyuridine, adenosine 5′-monophosphate, cytidine 5′-monophosphate, guanosine 5′-monophosphate, and uridine 5′-monophosphate. Then, a UPLC-QqQ/MS method was developed for the subsequent quantitative analysis. After validating the limits of quantification, detection, precision, repeatability, and recovery through a calibration curve, the content of 15 nucleic acid compounds was determined by the proposed UPLC-QqQ/MS method in 80 T. matsutake samples collected from different regions in Sichuan province, Southwest China. After the statistical analysis, we suggest that the total content of nucleic acid compounds in the qualified T. matsutake should be higher than 24.49 mg/100 g. The results indicated that the combined use of UPLC-TOF/MS and UPLC-QqQ/MS is efficient for fast identification and determination of nucleic acid compounds to comprehensively evaluate the quality of T. matsutake.

Characteristic Chromatogram: A Method of Discriminate and Quantitative Analysis for Quality Evaluation of Uncaria Stem with Hooks

Planta Medica ◽  
2017 ◽  
Vol 84 (06/07) ◽  
pp. 449-456 ◽  
Author(s):  
Jinjun Hou ◽  
Ruihong Feng ◽  
Yibei Zhang ◽  
Huiqin Pan ◽  
Shuai Yao ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Indole Alkaloids ◽  
Total Content ◽  
Traditional Chinese Medicines ◽  
Herbal Drugs ◽  
Chinese Medicines ◽  
Total Analysis Time ◽  
Total Analysis ◽  
Single Standard

AbstractIt remains a challenge to establish new monographs for herbal drugs derived from multiple botanical sources. Specifically, the difficulty involves discriminating and quantifying these herbs with components whose levels vary markedly among different samples. Using Uncaria stem with hooks as an example, a characteristic chromatogram was proposed to discriminate its five botanical origins and to quantify its characteristic components in the chromatogram. The characteristic chromatogram with respect to the components of Uncaria stem with hooks with the five botanical origins was established using 0.02% diethylamine and acetonitrile as the mobile phase. The total analysis time was 50 min and the detection wavelength was 245 nm. Using the same chromatogram parameters, the single standard to determine multicomponents method was validated to simultaneously quantify nine indole alkaloids, including vincosamide, 3α-dihydrocadambine, isocorynoxeine, corynoxeine, isorhynchophylline, rhynchophylline, hirsuteine, hirsutine, and geissoschizine methyl ether. The results showed that only the Uncaria stem with hooks from Uncaria rhynchophylla, the most widely used in the herbal market, showed the presence of these nine alkaloids. The conversion factors were 1.27, 2.32, 0.98, 1.04, 1.00, 1.02, 1.26, 1.33, and 1.25, respectively. The limits of quantitation were lower than 700 ng/mL. The total contents of 31 batches of Uncaria stem with hooks were in the range of 0.1 – 0.6%, except for Uncaria hirsuta Havil and Uncaria sinensis (Oliv.) Havil. The results also showed that the total content of indole alkaloids tended to decrease with an increase in the hook diameter. This showed that the characteristic chromatogram is practical for controlling the quality of traditional Chinese medicines with multiple botanical origins.

scholarly journals Simultaneous Determination of Six Active Compounds in Yixin Badiranjibuya Granules, a Traditional Chinese Medicine, by RP-HPLC-UV Method

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ning Yu ◽  
ChenHui He ◽  
Gulistan Awuti ◽  
Cheng Zeng ◽  
JianGuo Xing ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Rosmarinic Acid ◽  
Quality Evaluation ◽  
Scientific Basis ◽  
Determination Method ◽  
Rp Hplc ◽  
And Control ◽  
First Time

In this study, a sensitive, precise, and accurate HPLC-UV method was developed and validated to simultaneously determine the six analytes (luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, diosmetin-7-O-β-D-glucuronide, acacetin-7-O-β-D-glucuronide, tilianin, and rosmarinic acid) in Yixin Badiranjibuya Granules, in which five analytes (i.e., luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, diosmetin-7-O-β-D-glucuronide, acacetin-7-O-β-D-glucuronide, and rosmarinic acid) were determined for the first time in Yixin Badiranjibuya Granules, the content of tilianin in Yixin Badiranjibuya Granules was reported in other literatures, and the content of tilianin in our work was higher than that of the literature reports. The quality of 11 batch samples from four different manufacturers was evaluated using the proposed determination method. The contents of the six analytes were largely different among samples from various manufacturers. Therefore, this determination method can provide a scientific basis for quality evaluation and control of Yixin Badiranjibuya Granules.

scholarly journals Physical quality of seeds of Moquiniastrum polymorphum

2019 ◽  
Vol 79 (1) ◽  
pp. 63-69 ◽  
Author(s):  
J. C. T. Faria ◽  
L. A. Melo ◽  
C. R. M. Assumpção ◽  
G. E. Brondani ◽  
T. B. Breier ◽  
...  
Keyword(s):  
Heavy Fraction ◽  
External Evaluation ◽  
Physical Quality ◽  
X Ray ◽  
Internal Morphology ◽  
Combined Use ◽  
Heavy Fractions ◽  
Filled Seeds

Abstract This study aimed to analyze the physical properties of the seeds of Moquiniastrum polymorphum when processed by mini-SAS, X-ray and seed blower systems. The seeds were purchased from Seeds Caiçara, located in Penápolis-SP. After purchase, the seed lot was characterized by standard tests. In the X-ray test the seeds were classified according to their internal morphology as filled, unfilled or malformed. The seed blower was set at six opening settings, resulting in light and heavy fractions. The evaluations were performed by weighing the seeds that remained in the blower (heavy fraction) and by the amount of filled seeds indicated by the X-ray test. The germination tests were composed of four replicates of 50 seeds. The mini-SAS was used for external evaluation. It was concluded that the combined use of the seed blower and the X-ray test was efficient for the evaluation and determination of the physical quality of the seeds of M. polymorphum. The seed blower at opening settings 5 resulted in the best lot quality. The external parameters were not able to qualitatively evaluate the seeds of M. polymorphum.

Simultaneous Determination of Five Active Components in the Chinese Patent Medicine Niuhuang Jiangya Pill by HPLC-MS/MS

2017 ◽  
Vol 100 (3) ◽  
pp. 717-720
Author(s):  
Shan Xiong ◽  
Shanshan Lei
Keyword(s):  
Simultaneous Determination ◽  
Quality Evaluation ◽  
Gradient Elution ◽  
Astragaloside Iv ◽  
Active Components ◽  
Patent Medicines ◽  
Chinese Patent Medicine ◽  
Chinese Patent

Abstract Niuhuang Jiangya (NHJY) pill is one of the well-known Chinese patent medicines in China used in the treatment of high blood pressure. The primary purpose of this study was to establish and validate a method using HPLC with tandem MS for the quality evaluation of NHJY pill through simultaneous determination of the following five active components: baicalin, paeoniflorin, astragaloside IV, ferulic acid, and emodin. Chromatographic separation was carried out on a Hypersil GOLD HPLC C18 column (50 × 4.6 mm, 3 μm) with acetonitrileand water as mobile phase and gradient elution at a flow rateof 0.4 mL/min. The method established in this study was selective, linear, precise, and accurate and was successfully applied to evaluate five active components in NHJY pill collectedfrom different production batches, which could be considered a good approach to control the quality of NHJY pill and other related botanical drugs.

Determination of the Bearing Quality by Means of Vibroacoustic Response

2015 ◽  
Author(s):  
Stanislav Ziaran ◽  
Milos Musil ◽  
Ondrej Chlebo
Keyword(s):  
Quality Evaluation ◽  
Dynamical Response ◽  
Frequency Interval ◽  
Maximum Acceleration ◽  
Operational Conditions ◽  
Means Of Measurement ◽  
Damage Type ◽  
The Given

A signal of mechanical and acoustical vibration generated during a bearing’s quality testing contains variety of useful information about their operational conditions. At defined testing speeds, the bearings generate a periodical and a non-periodical vibroacoustic signal of different intensities, which are dependent on the quality of bearings and/or their damage type. A dynamical response of the tested bearings recorded in time characterizes also the nature of the unwanted noise of bearings. Therefore the dynamical response of the maximum acceleration recorded in time is one of the major criteria of bearing quality evaluation in terms of noisiness. The second criterion is a determination of statistically significant frequency interval for the equivalent acceleration value expressed in decibels. Consequently, from the effective acceleration value, the bearing quality can be determined in terms of its vibration severity, as well as its noise level. This objective methodology substitutes the evaluation bearing quality by means of measurement of the vibration acceleration on the given test device and simultaneously evaluation of the noise quality and its intensity auditorily. Verifying the proposed methodology 100 % conformity was achieved between the methodology currently used and the new methodology, which eliminates the subjective quality evaluation of bearings auditorily.

scholarly journals Relationship between the properties of raw and cooked spaghetti – new indices for pasta quality evaluation

2018 ◽  
Vol 32 (2) ◽  
pp. 217-223 ◽  
Author(s):  
Beata Biernacka ◽  
Dariusz Dziki ◽  
Renata Różyło ◽  
Monika Wójcik ◽  
Antoni Miś ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Strength Properties ◽  
Quality Parameters ◽  
Bending Test ◽  
Cooking Properties ◽  
Cooking Test ◽  
Colour Parameters ◽  
Important Quality

Abstract The quality of pasta can be evaluated by measuring the characteristics which encompass the most important quality parameters, such as colour, cooking properties and texture. The aim of the study was to suggest new indices which can be used to evaluate the quality of pasta. For the tests, 15 samples of spaghetti (produced from either semolina or common wheat flour) were used. The bending test was performed for the determination of the strength properties of raw pasta, while the pasta colour parameters were evaluated via the Commission Internationale de l’Eclairage system. The pasta cooking test included the evaluation of optimum cooking time, weight increase index and cooking loss. The samples of cooked spaghetti were cut, and the parameters describing pasta texture were determined. Statistical analysis showed significant correlations (α = 0.05) between colour parameters (lightness and redness) and pasta ash content (R = -0.90 and 0.84, respectively). The mechanical properties of raw pasta correlated positively with pasta density. The strongest correlation was found between pasta density and flexural strength. The destruction force for raw spaghetti during the bending test correlated significantly and positively with the cutting force of the cooked pasta. The obtained correlations can be helpful in pasta quality evaluation.

scholarly journals Determination of Flavonoid Glycosides by UPLC-MS to Authenticate Commercial Lemonade

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 3016 ◽  
Author(s):  
Ying Xue ◽  
Lin-Sen Qing ◽  
Li Yong ◽  
Xian-Shun Xu ◽  
Bin Hu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Low Cost ◽  
Ultra Performance Liquid Chromatography ◽  
Flavonoid Glycosides ◽  
Molecular Formula ◽  
Flight Mass Spectrometry ◽  
Dispersive Liquid Liquid Microextraction ◽  
Tof Ms

So far, there is no report on the quality evaluation of lemonade available in the market. In this study, a sample preparation method was developed for the determination of flavonoid glycosides by ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) based on vortex-assisted dispersive liquid-liquid microextraction. First, potential flavonoids in lemonade were scanned and identified by ultra-performance liquid chromatography–time of flight mass spectrometry (UPLC-TOF/MS). Five flavonoid glycosides were identified as eriocitrin, narirutin, hesperidin, rutin, and diosmin according to the molecular formula provided by TOF/MS and subsequent confirmation of the authentic standard. Then, an ultra-performance liquid chromatography–triple quadrupole mass spectrometry (UPLC-QqQ/MS) method was developed to determine these five flavonoid glycosides in lemonade. The results showed that the content of rutin in some lemonade was unreasonably high. We suspected that many illegal manufacturers achieved the goal of low-cost counterfeiting lemonade by adding rutin. This suggested that it was necessary for relevant departments of the state to make stricter regulations on the quality standards of lemonade beverages.

scholarly journals Comparison of Volatiles and Chemical Composition of Traditional and Non-Traditional Honey Available on the Polish Market

2021 ◽  
Vol 11 (14) ◽  
pp. 6371
Author(s):  
Dorota Derewiaka ◽  
Ewa Majewska ◽  
Katarzyna Kuzak ◽  
Dominika Szadkowska
Keyword(s):  
Volatile Compounds ◽  
Total Content ◽  
Isovaleric Acid ◽  
Total Phenols ◽  
European Law ◽  
Butanoic Acid ◽  
Aroma Profile ◽  
Cation Radicals

The purpose of the work was to compare the quality of selected honey available on the Polish market, including traditional (rape, lime and meadow and marsh honey) and non-traditional honey (lime, buckwheat, and honeydew honey from coniferous honeydew). Parameters such as electrical conductivity, color, pH, acidity, water, hydroxymethylfurfural, total phenols content, and ability to deactivate ABTS cation radicals were determined. The profile of aroma compounds was carried out by GC-MS technique, and determination of sugars was performed by HPLC. It was found that all tested honey met standards according to European law requirements. Semi-quantitative analysis of volatile compounds showed that all honey samples contain numerous volatiles (in buckwheat honey there were 67 compounds, and in honeydew honey from coniferous honeydew, only 40 compounds). Characteristic volatile compounds of each aroma profile were described e.g., benzaldehyde, acetone, 2-methyl-butanal, nonanal, benzyl alcohol were found in rape honey aroma, and furfural, isovaleric acid, ethanol, delta-valerolactone, isovaleraldehyde, 2-methyl-butanoic acid, and phenylacetaldehyde in buckwheat honey aroma. The total content of volatiles was the highest in buckwheat honey (199.62 µg/kg), and in traditional lime honey (195.17 µg/kg). The lowest total content of volatile substances was established in non-traditional lime honey (73.20 µg/kg) and in rape honey (39.52 µg/kg).

scholarly journals Structural Identification and Conversion Analysis of Malonyl Isoflavonoid Glycosides in Astragali Radix by HPLC Coupled with ESI-Q TOF/MS

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3929 ◽  
Author(s):  
Yunfeng Zheng ◽  
Weiping Duan ◽  
Jie Sun ◽  
Chenguang Zhao ◽  
Qizhen Cheng ◽  
...  
Keyword(s):  
Total Content ◽  
Astragali Radix ◽  
Fragment Ions ◽  
Long Duration ◽  
Diagnostic Fragment ◽  
Positive Mode ◽  
Relationship Of ◽  
The Relationship ◽  
Tof Ms

In this study, four malonyl isoflavonoid glycosides (MIGs), a type of isoflavonoid with poor structural stability, were efficiently isolated and purified from Astragali Radix by a medium pressure ODS C18 column chromatography. The structures of the four compounds were determined on the basis of NMR and literature analysis. Their major diagnostic fragment ions and fragmentation pathways were proposed in ESI/Q-TOF/MS positive mode. Using a target precursor ions scan, a total of 26 isoflavonoid compounds, including eleven malonyl isoflavonoid glycosides coupled with eight related isoflavonoid glycosides and seven aglycones were characterized from the methanolic extract of Astragali Radix. To clarify the relationship of MIGs and the ratio of transformation in Astragali Radix under different extraction conditions, two MIGs (calycosin-7-O-glycoside-6″-O-malonate and formononetin-7-O-glycoside-6″-O-malonate) coupled with related glycosides (calycosin-7-O-glycoside and formononetin-7-O-glycoside) and aglycones (calycosin and formononetin) were detected by a comprehensive HPLC-UV method. Results showed that MIGs could convert into related glycosides under elevated temperature conditions, which was further confirmed by the conversion experiment of MIGs reference compounds. Moreover, the total contents of MIGs and related glycosides displayed no obvious change during the long-duration extraction. These findings indicated that the quality of Astragali Radix could be evaluated efficiently and accurately by using the total content of MIGs and related glycosides as the quality index.

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