scholarly journals Cordycepin Isolated from Cordyceps militaris: Its Newly Discovered Herbicidal Property and Potential Plant-Based Novel Alternative to Glyphosate

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2901 ◽  
Author(s):  
Quy ◽  
Xuan ◽  
Andriana ◽  
Tran ◽  
Khanh ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Plant Growth ◽  
Growth Inhibitor ◽  
Cordyceps Militaris ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Ionization Mass ◽  
Aerial Parts ◽  
Plant Growth Inhibitor ◽  
Germination And Growth

There is currently much interest in finding new phytochemicals among plants and fungi as nature-based alternatives to replace problematic herbicides such as glyphosate, which are preferentially used in agricultural production n. We discovered striking herbicidal potency in Cordyceps militaris (L.) and identified cordycepin as its principal plant growth inhibitor. Cordycepin obtained as an ethyl acetate extract was subjected to column chromatography and evaluated for its bioassay-guided phytotoxic capacity against Raphanus sativus (radish), showing a maximum inhibition on germination and growth of radish (IC50 = 0.052–0.078 mg/mL). Gas chromatography-mass spectrometry (GC-MS) (m/z: 251.2) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) ([M + Na]+ m/z: 274.1; [M + H]+ m/z: 252.1) analyses confirmed cordycepin as the major component of the tested column fraction (55.38%). At 0.04 mg/mL, cordycepin showed 3.8–5.9- and 3.3–3.7-fold greater inhibition of the germination and growth of radish than benzoic acid (BA) and glyphosate, respectively. Compared with BA, isolated cordycepin reduced plant chlorophyll and carotenoid contents (2.0–9.5 -fold), while proline, total phenolic and total flavonoid contents were increased 1.2–1.8-fold. Finally, cordycepin promoted electrolyte leakage and malondialdehyde accumulation in radish aerial parts. Thus, cordycepin successfully isolated from Cordyceps militaris is a highly potent plant growth inhibitor with pending worldwide patent and may become a potential plant-based novel alternative to the disputed glyphosate.

scholarly journals Discovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takashi Ishida ◽  
Haruna Yoshimura ◽  
Masatsugu Takekawa ◽  
Takumi Higaki ◽  
Takashi Ideue ◽  
...  
Keyword(s):  
Plant Growth ◽  
Human Life ◽  
Growth Inhibitor ◽  
Herbicidal Activity ◽  
Functional Improvement ◽  
Microtubule Inhibitors ◽  
Plant Microtubules ◽  
Plant Growth Inhibitor ◽  
Derivatives Of ◽  
Synthetic Intermediate

AbstractThe discovery and useful application of natural products can help improve human life. Chemicals that inhibit plant growth are broadly utilized as herbicides to control weeds. As various types of herbicides are required, the identification of compounds with novel modes of action is desirable. In the present study, we discovered a novelN-alkoxypyrrole compound, kumamonamide fromStreptomyces werraensisMK493-CF1 and established a total synthesis procedure. Resulted in the bioactivity assays, we found that kumamonamic acid, a synthetic intermediate of kumamonamide, is a potential plant growth inhibitor. Further, we developed various derivatives of kumamonamic acid, including a kumamonamic acid nonyloxy derivative (KAND), which displayed high herbicidal activity without adverse effects on HeLa cell growth. We also detected that kumamonamic acid derivatives disturb plant microtubules; and additionally, that KAND affected actin filaments and induced cell death. These multifaceted effects differ from those of known microtubule inhibitors, suggesting a novel mode of action of kumamonamic acid, which represents an important lead for the development of new herbicides.

Analysis of DCM extractable components from hot-pressed hybrid poplar

Holzforschung ◽  
2012 ◽  
Vol 66 (8) ◽  
pp. 927-934 ◽  
Author(s):  
Noridah B. Osman ◽  
Armando G. McDonald ◽  
Marie-Pierre G. Laborie
Keyword(s):  
Mass Spectrometry ◽  
Hybrid Poplar ◽  
Gel Permeation Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Wood Substrate ◽  
Thermal Compression ◽  
Organic Extracts ◽  
Extractable Compounds ◽  
Soluble Material

Abstract The effects of thermal compression on the organic-soluble material of a uniform wood substrate, hybrid poplar (clone OP-367), has been studied. Poplar veneers were preconditioned to 0% or 8% moisture content and subsequently hot pressed at 150°C, 200°C, and 250°C. The dichloromethane (DCM) extracts were characterized by various hyphenated analyses [gas chromatography-mass spectrometry (GC-MS), analytical pyrolysis (Py-GC-MS), electrospray ionization-mass spectrometry (ESI-MS), and gel permeation chromatography (GPC)]. The yields and composition of extractable compounds were markedly influenced by treatment at 250°C compared to those obtained at lower temperatures. Most importantly, the organic extracts contained a significant portion of lignin-derived compounds after treatment at 250°C.

Xanthoxin, a recently discovered plant growth inhibitor

1972 ◽  
Vol 180 (1060) ◽  
pp. 317-346 ◽  
Keyword(s):  
Plant Growth ◽  
Growth Inhibitor ◽  
Wheat Coleoptile ◽  
Spectroscopic Techniques ◽  
Plant Growth Inhibitor ◽  
Coleoptile Section ◽  
Cress Seed ◽  
Aba Content ◽  
Seed Germination Test

The naturally occurring plant growth inhibitor xanthoxin which was discovered in these laboratories has been prepared in vitro by the oxidation of the pigment violaxanthin with neutral zinc permanganate solution. By the use of chemical and spectroscopic techniques, xanthoxin has been characterized as a mixture of the 2- cis , 4- trans - and 2- trans , 4- trans -isomers of 5-(1', 2', -epoxy-4'-hydroxy-2', 6', 6'-tri-methyl-1'-cyclohexy)-3-methyl-pentadienal. These are also obtained by similar oxidations of neoxanthin, antheraxanthin and lutein epoxide. Cis, trans -xanthoxin probably arises from the corresponding cis -xanthophyll and its conversion to ( + )-ABA by simple chemical procedures is reported. The biological activity of cis, trans -xanthoxin has been shown to be considerably greater than that of the trans, trans -isomer. It is comparable with that of ( ± )-ABA in the wheat coleoptile section, the lettuce hypocotyl and bean petiole abscission tests, but greater than that of ( ± )-ABA in the cress seed germination test. Xanthoxin is shown to antagonize the growth-promoting effects of the plant hormones 3-indolylacetic acid, gibberellic acid and kinetin. Other xanthoxin derivatives have been prepared and their activities in several tests are also reported. The uptake of xanthoxin by tomato shoots leads to a subsequent large increase in the ABA content of the tissue. Evidence is presented for a possible biogenetic conversion of cis, trans -xanthoxin into ( + )-ABA by plants.

scholarly journals Chemical composition and antibacterial activities of essential oils from fruits of Melicope pteleifolia (Champ. Ex Benth.) T.G. Hartley grown in Lam Dong Province, Vietnam

2020 ◽  
Vol 42 (3) ◽  
Author(s):  
Hoang Thi Binh ◽  
Tran Thi Bao Tram ◽  
Do Ngoc Dai ◽  
Vuong Thuy Tien ◽  
Le Minh Tam ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Composition ◽  
Essential Oil ◽  
Streptococcus Pyogenes ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Aerial Parts ◽  
Agar Well Diffusion Method

In the present study, chemical composition and antibacterial properties of essential oil obtained from the aerial parts of the Melicope pteleifolia (Champ. ex Benth.) T.G Hartley in Dalat were evaluated. Essential oil was isolated through hydro-distillation. Twenty-nine constituents comprising 100% of the essential oil were characterized by gas chromatography/mass spectrometry (GC-MS) techniques. The major compounds in the essential oil were (+)-Sabinene (34.73%), Cis-α-bergamotene (13.15%), Z-α-trans-bergamotol (5.28%), β-mycrene (4.98%), and 1,3,6-octatriene, 3,7-dimethyl-(4.71%). Antibacterial activities of Melicope pteleifolia essential oil were investigated against Gram-positive and-negative bacteria. Results showed significant activities against Streptococcus pyogenes and Escherichia coli using an agar well diffusion method. The application of this essential oil in preventing and eliminating bacteria could be useful in fields as medicine and cosmetics. 

scholarly journals Aroma retention during drying of caja-umbu fruit pulp

2018 ◽  
Author(s):  
Narendra Narain ◽  
Anderson Santos Fontes ◽  
Maria Terezinha Santos Leite-Neta ◽  
Patricia Nogueira Matos ◽  
Hannah Caroline Santos Araújo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Phenolic Compounds ◽  
Volatile Compounds ◽  
Spray Drying ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Fruit Pulp ◽  
Total Phenolic Compounds ◽  
Different Temperatures

This study was aimed to obtain and characterize the dried powder of cajá-umbu (Spondias spp) fruit pulp obtained by spray-drying and lyophilization. Spray-drying of the pulp was done at different temperatures. Analysis of bioactive compounds and volatile compounds was performed. The total phenolic compounds content was high in the dried powder obtained at the temperature of 140 °C. The volatiles analysis of dried powders revealed  that the powder dried at  140°C contained a larger number of compounds. The cajá-umbu powder showed that it is a better alternative for storage and conservation since it retained the majority of volatile compounds. Keywords: Cajá-umbu, volatile compounds, gas chromatography, mass spectrometry.

scholarly journals Pharmacological perspectives from Brazilian Salvia officinalis (Lamiaceae): antioxidant, and antitumor in mammalian cells

2016 ◽  
Vol 88 (1) ◽  
pp. 281-292 ◽  
Author(s):  
CHARLENE S.C. GARCIA ◽  
CAROLINE MENTI ◽  
ANA PAULA F. LAMBERT ◽  
THIAGO BARCELLOS ◽  
SIDNEI MOURA ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mammalian Cells ◽  
Annexin V ◽  
The Body ◽  
Salvia Officinalis ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Hek 293 ◽  
Salvia Officinalis L

ABSTRACT Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identification sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia officinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, inflammation and cancer.

scholarly journals Analytical Variables Affecting Analysis of F2-Isoprostanes and F4-Neuroprostanes in Human Cerebrospinal Fluid by Gas Chromatography/Mass Spectrometry

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Hsiu-Chuan Yen ◽  
Hsing-Ju Wei ◽  
Ting-Wei Chen
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Chemical Ionization ◽  
Negative Ion ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Negative Ion Chemical Ionization ◽  
Gold Marker

F2-isoprostanes (F2-IsoPs) are a gold marker of lipid peroxidationin vivo, whereas F4-neuroprostanes (F4-NPs) measured in cerebrospinal fluid (CSF) or brain tissue selectively indicate neuronal oxidative damage. Gas chromatography/negative-ion chemical-ionization mass spectrometry (GC/NICI-MS) is the most sensitive and robust method for quantifying these compounds, which is essential for CSF samples because abundance of these compounds in CSF is very low. The present study revealed potential interferences on the analysis of F2-IsoPs and F4-NPs in CSF by GC/NICI-MS due to the use of improper analytical methods that have been employed in the literature. First, simultaneous quantification of F2-IsoPs and F4-NPs in CSF samples processed for F4-NPs analysis could cause poor chromatographic separation and falsely higher F2-IsoPs values for CSF samples with high levels of F2-IsoPs and F4-NPs. Second, retention of unknown substances in GC columns from CSF samples during F4-NPs analysis and from plasma samples during F2-IsoPs analysis might interfere with F4-NPs analysis of subsequent runs, which could be solved by holding columns at a high temperature for a period of time after data acquisition. Therefore, these special issues should be taken into consideration when performing analysis of F2-IsoPs and F4-NPs in CSF to avoid misleading results.

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