scholarly journals Terpenoids and Phytocannabinoids Co-Produced in Cannabis Sativa Strains Show Specific Interaction for Cell Cytotoxic Activity

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3031 ◽  
Author(s):  
Dvora Namdar ◽  
Hillary Voet ◽  
Vinayaka Ajjampura ◽  
Stalin Nadarajan ◽  
Einav Mayzlish-Gati ◽  
...  
Keyword(s):  
Biological Activity ◽  
Secondary Metabolites ◽  
Cytotoxic Activity ◽  
Cannabis Sativa ◽  
Specific Interaction ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Cannabidiolic Acid ◽  
Hct 116 ◽  
Analytical Hplc

Mixtures of different Cannabis sativa phytocannabinoids are more active biologically than single phytocannabinoids. However, cannabis terpenoids as potential instigators of phytocannabinoid activity have not yet been explored in detail. Terpenoid groups were statistically co-related to certain cannabis strains rich in Δ9-tetrahydrocannabinolic acid (THCA) or cannabidiolic acid (CBDA), and their ability to enhance the activity of decarboxylase phytocannabinoids (i.e., THC or CBD) was determined. Analytical HPLC and GC/MS were used to identify and quantify the secondary metabolites in 17 strains of C. sativa, and correlations between cannabinoids and terpenoids in each strain were determined. Column separation was used to separate and collect the compounds, and cell viability assay was used to assess biological activity. We found that in “high THC” or “high CBD” strains, phytocannabinoids are produced alongside certain sets of terpenoids. Only co-related terpenoids enhanced the cytotoxic activity of phytocannabinoids on MDA-MB-231 and HCT-116 cell lines. This was found to be most effective in natural ratios found in extracts of cannabis inflorescence. The correlation in a particular strain between THCA or CBDA and a certain set of terpenoids, and the partial specificity in interaction may have influenced the cultivation of cannabis and may have implications for therapeutic treatments.

Spectroscopic Identifi cation of New Ellagitannins and a Trigalloylglucosylkaempferol from an Extract of Euphorbia cotinifolia L. with Antitumour and Antioxidant Activity

2012 ◽  
Vol 67 (3-4) ◽  
pp. 151-162
Author(s):  
Mohamed S. Marzouk ◽  
Fatma A. Moharram ◽  
Amira Gamal-Eldeen ◽  
Iman M. Damlakhy
Keyword(s):  
Antitumour Activity ◽  
2D Nmr ◽  
Chloroform Extract ◽  
Biological Evaluation ◽  
Carcinoma Cells ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Moderate Growth ◽  
Chemical Studies ◽  
Hct 116

5From an extract of leaves and small branches of Euphorbia cotinifolia L., 17 polyphenols were isolated including two new ellagitannins and a trigalloyl-glucosylkaempferol. Based on extensive spectral data (UV, ESI-MS, 1H NMR, DEPT and 1D/2D NMR) and chemical studies, their structures were characterized as 1-O-galloyl-3,6-hexahydroxydiphenoyl-DB1,4- glucopyranose (), 1-O-galloyl-3,6-valoneoyl-D-B1,4-glucopyranose (6), and kaempferol 3-O-(2”,3”,6”-tri-O-galloyl)-β-D-glucopyranoside (13). Biological evaluation indicated that the 80% aqueous methanol extract (AME), chloroform extract (CE), and some pure compounds have potent scavenging activity in the DPPH assay with SC50 values lower than that of ascorbic acid, especially 5, 7 - 9, and a mixture of hyperin 6”-gallate (11) and isoquercitrin 6”-gallate (12). Moreover, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell viability assay, 6 and 8 exhibited the highest inhibition of human hepatocellular carcinoma cells (Hep-G2), while AME, CE, 5, 7, 9, and the mixture of 11 and 12 were found to be moderate growth inhibitors according to their IC50 values. In addition, AME, 5, and 8 exhibited significant antiproliferative activity against colon carcinoma cells (HCT-116); however, CE and the other examined compounds displayed moderate to low antitumour activity against HCT-116 cells

Antioxidant and cytotoxic activity of three Turkish marine-derived fungi

2019 ◽  
Vol 44 (4) ◽  
pp. 554-559
Author(s):  
Bülent Gözcelioğlu
Keyword(s):  
Secondary Metabolites ◽  
Cytotoxic Activity ◽  
Marine Invertebrates ◽  
Therapeutic Potential ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Cytotoxicity Activity ◽  
Bioactive Secondary Metabolites ◽  
Hct 116

Abstract Background Marine-derived fungi are appraised as a favorable source for discovering new bioactive secondary metabolites. In the last few decades researchers have concentrated on marine-derived fungi to obtain new and pharmaceutically active bioactive secondary metabolites with therapeutic potential. Objective In this study three marine-derived fungi were isolated and identified from marine invertebrates and investigated with regard to their antioxidant and cytotoxic activities. Materials and methods DPPH, SO, NO, and ABTS assays were used for monitoring free radical scavenging activity, and the MTT assay was used for testing cytotoxic activity against HCT-116 colon cancer cells. Results According to the obtained results Malassezia restricta extract was shown to have the highest antioxidant and cytotoxic activities compared to the other tested fungi strains. Conclusion This study is the first report about the antioxidant and cytotoxicity activity of Acremonium sclerotigenum, Aspergillus flavus, and M. restricta. This serves as a valuable preliminary study for activity-guided isolation of secondary metabolites.

LED lighting affects the composition and biological activity of Cannabis sativa secondary metabolites

2019 ◽  
Vol 132 ◽  
pp. 177-185 ◽  
Author(s):  
Dvory Namdar ◽  
Dana Charuvi ◽  
Vinayka Ajjampura ◽  
Moran Mazuz ◽  
Aurel Ion ◽  
...  
Keyword(s):  
Biological Activity ◽  
Secondary Metabolites ◽  
Cannabis Sativa ◽  
Led Lighting

Design, Synthesis and Biological Evaluation of Novel Diaryl Pyrazole Derivatives as Anticancer Agents

2020 ◽  
Vol 17 (3) ◽  
pp. 216-223
Author(s):  
Jalal Nourmahammadi ◽  
Ebrahim Saeedian Moghadam ◽  
Zahra Shahsavari ◽  
Mohsen Amini
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Annexin V ◽  
Biological Evaluation ◽  
Pyrazole Derivatives ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Cancerous Cell

Cancer is one of the major causes of mortality all around the world. Globally, nearly 1 in 6 deaths is due to cancer. Researchers are trying to synthesize new anticancer agents. Previous studies demonstrated that some pyrazole derivatives could be considered as potential anticancer agents. Herein, ten novel derivatives of 1,5-diarylpyrazole were synthesized in four step reactions and cytotoxic activity was investigated by MTT cell viability assay. All of the compounds were characterized by 1H NMR and 13C NMR and their purity was confirmed by elemental analysis. The cytotoxicity was determined against three cancerous cell lines (HT-29, U87MG and MDA-MB 468) and AGO1522 as a normal cell line. Compound 5a showed the best cytotoxic activity on cancerous cell lines in comparison to paclitaxel. Annexin V/ PI staining assay also showed that compounds 5a and 5i would lead to significant apoptosis induction in MDA-MB 486 cell line.

scholarly journals CELL VIABILITY ASSAY OF FICUS BENGHALENSIS LATEX SOLVENT EXTRACTS ON DIFFERENT CELL LINES

2018 ◽  
Vol 11 (10) ◽  
pp. 335
Author(s):  
Tulasi Cdsln ◽  
Lakshmi Narasu M ◽  
Saida L
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Morphological Changes ◽  
Ethanol Extract ◽  
Peripheral Blood Lymphocytes ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Ficus Benghalensis ◽  
Diphenyltetrazolium Bromide ◽  
Hct 116

Objective: Presented here in the study, the screening for antiproliferative activity of Ficus benghalensis dried latex solvent extracts on human breast MDA MB 231, colorectal HCT116, and neuroblastoma IMR 32 cell lines.Methods: The anticancer activity of ethanol, methanol, ethyl acetate, and acetone extracts against the above-mentioned cancer cell lines as well in lymphocytes, by 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay and propidium iodide staining was used to observe the morphological changes occurred in the cell due to the affect of latex extract.Results: Among all the extracts, ethanol extract was found to be effective against IMR 32 and HCT 116 whereas ethyl acetate extract in case of MDA MB 231 cell line with 50% inhibitory concentration 50% (IC50) 123.27±2.5 μg/ml, 99.82±9.06 μg/ml, and 75.66± 6.3, respectively.Conclusion: The extracts were found to be less toxic on peripheral blood lymphocytes. The IC50 value of the cytotoxic activity measured using MTT dye indicated that the extracts were efficient in inhibition of the cell proliferation of these cell lines.

Design, Synthesis and Anticancer Evaluation of Novel Series of Indibulin Analogues

2019 ◽  
Vol 15 (3) ◽  
pp. 231-239
Author(s):  
Ebrahim S. Moghadam ◽  
Farhad Saravani ◽  
Seyednasser Ostad ◽  
Shohreh Tavajohi ◽  
Morteza P. Hamedani ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Normal Cell ◽  
Annexin V ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Cancerous Cell ◽  
Ht 29

Background: Cancer is an important cause of human death worldwide. During the last decades, many anticancer agents with anti-tubulin mechanism have been synthesized or extracted from nature and some of them also entered clinical use. Indibulin is one of the most potent tubulin polymerization inhibitors with minimal peripheral neuropathy, which is a big problem by some of the antimitotic agents such as taxanes and vinka alkaloids. With respect to this giant benefit, herein we decided to design and synthesize novel indibulin related compounds and investigate their anticancer activity against HT-29, Caco-2 and T47-D cancerous cell lines as well as NIH-T3T as normal cell line. Objective: The aim of this study was to synthesize new anti-cancer agents and evaluates their cytotoxic activity on diverse cancerous and normal cell lines. Method: Target compounds were synthesized in multistep reaction and cytotoxic activity was investigated by MTT cell viability assay. Results: Herein, nine novel target compounds were synthesized in moderate to good yield. Some of the compounds exerted good cytotoxic activity against cancerous cell lines. Annexin V/PI staining showed that compound 4g could induce apoptosis and necrosis in HT-29 cell line. Conclusion: It is valuable to do further investigation on compound 4g which showed the highest activity against HT-29 and Caco-2 (IC50 values are 6.9 and 7 &µM respectively). Also, synthesis of new derivatives of current synthesized compounds is suggested.

Proliferative Effect of Tilapia Fish (Oreochromis niloticus) Lectin on BALB/c Mice Splenocytes

2019 ◽  
Vol 26 (12) ◽  
pp. 887-892
Author(s):  
Cynarha Daysy Cardoso da Silva ◽  
Cristiane Moutinho Lagos de Melo ◽  
Elba Verônica Matoso Maciel Carvalho ◽  
Mércia Andréa Lino da Silva ◽  
Rosiely Félix Bezerra ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Oreochromis Niloticus ◽  
Cytokine Production ◽  
Thymidine Incorporation ◽  
Con A ◽  
Cell Viability Assay ◽  
Proliferative Effect ◽  
Viability Assay ◽  
Apoptosis And Necrosis

Background: Lectins have been studied in recent years due to their immunomodulatory activities. Objective: We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes. Methods: Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine incorporation, cytokines were investigated using ELISA assay and cell viability assay was performed by investigation of damage through signals of apoptosis and necrosis. Results: OniL did not promote significant cell death, induced high mitogenic activity in relation to control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines. Conclusion: These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent in immunostimulatory assays.

Do We have a Satisfactory Cell Viability Assay? Review of the Currently Commercially-Available Assays

2020 ◽  
Vol 17 (1) ◽  
pp. 2-22 ◽  
Author(s):  
Abdel-Baset Halim
Keyword(s):  
Cell Viability ◽  
Data Interpretation ◽  
Positive Control ◽  
Live Cells ◽  
Proof Of Concept ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Current Cell ◽  
Dying Cells ◽  
Differential Permeability

:Cell-based assays are an important part of the drug discovery process and clinical research. One of the main hurdles is to design sufficiently robust assays with adequate signal to noise parameters while maintaining the inherent physiology of the cells and not interfering with the pharmacology of target being investigated.:A plethora of assays that assess cell viability (or cell heath in general) are commercially available and can be classified under different categories according to their concepts and principle of reactions. The assays are valuable tools, however, suffer from a large number of limitations. Some of these limitations can be procedural or operational, but others can be critical as those related to a poor concept or the lack of proof of concept of an assay, e.g. those relying on differential permeability of dyes in-and-out of viable versus compromised cell membranes. While the assays can differentiate between dead and live cells, most, if not all, of them can just assess the relative performance of cells rather than providing a clear distinction between healthy and dying cells. The possible impact of relatively high molecular weight dyes, used in most of the assay, on cell viability has not been addressed. More innovative assays are needed, and until better alternatives are developed, setup of current cell-based studies and data interpretation should be made with the limitations in mind. Negative and positive control should be considered whenever feasible. Also, researchers should use more than one orthogonal method for better assessment of cell health.

scholarly journals Non-Cannabinoid Metabolites of Cannabis sativa L. with Therapeutic Potential

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 400
Author(s):  
Henry Lowe ◽  
Blair Steele ◽  
Joseph Bryant ◽  
Ngeh Toyang ◽  
Wilfred Ngwa
Keyword(s):  
Secondary Metabolites ◽  
Cannabis Sativa ◽  
Therapeutic Potential ◽  
Physiological Activity ◽  
Economic Value ◽  
Therapeutic Window ◽  
Future Directions ◽  
Medicinal Properties ◽  
The Many ◽  
Human Viruses

The cannabis plant (Cannabis sativa L.) produces an estimated 545 chemical compounds of different biogenetic classes. In addition to economic value, many of these phytochemicals have medicinal and physiological activity. The plant is most popularly known for its two most-prominent and most-studied secondary metabolites—Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD). Both Δ9-THC and CBD have a wide therapeutic window across many ailments and form part of a class of secondary metabolites called cannabinoids—of which approximately over 104 exist. This review will focus on non-cannabinoid metabolites of Cannabis sativa that also have therapeutic potential, some of which share medicinal properties similar to those of cannabinoids. The most notable of these non-cannabinoid phytochemicals are flavonoids and terpenes. We will also discuss future directions in cannabis research and development of cannabis-based pharmaceuticals. Caflanone, a flavonoid molecule with selective activity against the human viruses including the coronavirus OC43 (HCov-OC43) that is responsible for COVID-19, and certain cancers, is one of the most promising non-cannabinoid molecules that is being advanced into clinical trials. As validated by thousands of years of the use of cannabis for medicinal purposes, vast anecdotal evidence abounds on the medicinal benefits of the plant. These benefits are attributed to the many phytochemicals in this plant, including non-cannabinoids. The most promising non-cannabinoids with potential to alleviate global disease burdens are discussed.

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