Hyalodendrins A and B, New Decalin-Type Tetramic Acid Larvicides from the Endophytic Fungus Hyalodendriella sp. Ponipodef12

Two new decalin/tetramic acid hybrid metabolites, hyalodendrins A (1) and B (2) were isolated from plant endophytic fungus Hyalodendriella sp. Ponipodef12. The structures of the new compounds were elucidated by analysis of the spectroscopic data, including NMR, HRMS and ECD, and by chemical conversion. Compounds 1 and 2 were phomasetin analogues, and both showed potent larvicidal activity against the fourth-instar larvae of Aedes aegypti with the median lethal dose (LC50) values of 10.31 and 5.93 μg/mL, respectively.

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Cytochalasins from Xylaria sp. CFL5, an Endophytic Fungus of Cephalotaxus fortunei

Natural Products and Bioprospecting ◽

10.1007/s13659-020-00279-5 ◽

2020 ◽

Author(s):

Kai-Liang Ma ◽

Shi-Hui Dong ◽

Hang-Ying Li ◽

Wen-Jun Wei ◽

Yong-Qiang Tu ◽

...

Keyword(s):

Endophytic Fungus ◽

Spectroscopic Data ◽

Biological Activities ◽

Optical Rotation ◽

Ags Cells ◽

Mhc Ii ◽

Chemical Structures ◽

Data Analyses ◽

Binding Inhibition ◽

New Compounds

Abstract Three previously undescribed cytochalasins, named xylariasins A‒C (1‒3), together with six known ones (4‒9) were isolated from Xylaria sp. CFL5, an endophytic fungus of Cephalotaxus fortunei. The chemical structures of all new compounds were elucidated on the basis of extensive spectroscopic data analyses and electronic circular dichroism calculation, as well as optical rotation calculation. Biological activities of compounds 1, 4‒9 were evaluated, including cytotoxic, LAG3/MHC II binding inhibition and LAG3/FGL1 binding inhibition activities. Compounds 6 and 9 possessed cytotoxicity against AGS cells at 5 μM, with inhibition rates of 94% and 64%, respectively. In addition, all tested isolates, except compound 6, exhibited obvious inhibitory activity against the interaction of both LAG3/MHC II and LAG3/FGL1. Compounds 1, 5, 7, and 8 inhibited LAG3/MHC II with IC50 values ranging from 2.37 to 4.74 μM. Meanwhile, the IC50 values of compounds 1, 7, and 8 against LAG3/FGL1 were 11.78, 4.39, and 7.45 μM, respectively. Graphic Abstract

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Larvicidal activity of microparticles of Melissa officinalis L. essential oil (Lamiaceae) against Aedes aegypti (Diptera, Culicidae)

Research Society and Development ◽

10.33448/rsd-v10i1.11166 ◽

2021 ◽

Vol 10 (1) ◽

pp. e35710111166

Author(s):

Thércia Gabrielle Teixeira Martins ◽

Paulo Victor Serra Rosa ◽

Mariana Oliveira Arruda ◽

Andressa Almeida Santana Dias ◽

Ari Pereira de Araújo Neto ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Fourth Instar ◽

Tween 20 ◽

Distilled Water ◽

Melissa Officinalis ◽

The Face ◽

Muench Method

This study aimed to evaluate the larvicidal activity of essential oil microparticles (EO) of Melissa officinalis L. against Aedes aegypti. The leaves of M. officinalis were collected in the municipality of São José de Ribamar, Maranhão, Brazil, later dried, crushed and ground. 90g of the dried leaves were used to obtain the EO by the hydrodistillation method. For the synthesis of microencapsulated EO, 60g of sodium alginate (2.5% m/v) was added to the mixture of 15g of Tween 20 with 6g of EO. The mixture was homogenized and drips over CaCl2 5% m/v solution for the hardening of particles via crosslinking. The microparticles were washed with distilled water in filter and dried at 35ºC/24h and 15 days at tamb (30ºC). The eggs of Aedes aegypti were collected at the Federal University of Maranhão by the ovitrampas method. The larvae that hatched were fed until they reached the fourth instar. Groups of larvae (n=20) were submitted to solutions of EO and microparticles of 10-90 mg/L . After 24 h, live and dead larvae were counted and LC50 was calculated by the Reed&Muench method, using Cheng's criterion for classification of active potential. All larvae presented mortality in all concentrations tested. The LC50 obtained for the EO was 40.60 mg/L and for the microparticles 22.10 mg/L, both classified as active according to the adopted criterion, but it is observed that the microparticles increased the larvicidal potential of the EO. Through the results obtained, it is concluded that the microparticles formulated with the EO proved to be efficient in the face of the larvae of Aedes aegypti, being interesting and important in controlling and combating the mosquito that transmits dengue.

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Larvicidal and Oviposition Activity of Against Vector Aedes Aegypti and Molecular Docking Studies of Metabolites from the Crude Extract of the Endophytic Fungus Aspergillus Sp. Isolated from Bertholletia Excelsa Humn. & Bonpl.

10.21203/rs.3.rs-535443/v1 ◽

2021 ◽

Author(s):

Inana F. Araújo ◽

Victor Hugo Marinho ◽

Iracirema S Sena ◽

Jhone Curti ◽

Ryan S. Ramos ◽

...

Keyword(s):

Molecular Docking ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Crude Extract ◽

Endophytic Fungus ◽

Docking Studies ◽

Resistant Bacteria ◽

Bertholletia Excelsa ◽

Molecular Docking Studies ◽

Biological Potential

Abstract This work showed the crude extract of the endophytic fungus Aspergillus sp, isolated from the almonds of Bertholletia excelsa Humn & Bonlp collected in the Brazilian Amazon, oviposition deterrent, and larvicidal activity of against Aedes aegypti. In the oviposition deterrence test was observed that females able to lay eggs preferred the control oviposition sites (46.6%), suggesting the extract also could repel the oviposition. Futhermore, the extract showed larvicidal activity with LC50 26.86 µg/mL at 24 hours and 18.75 µg/mL at 48 hours. Molecular docking studies were carried out to elucidate the mechanism of action of the compounds identified against the enzyme acetylcholinesterase. The compound Aspergillol B was a potent larvicide with potential for inhibition for the acetylcholinesterase enzyme (-7.74 Kcal/mol). These unprecedented results reported indicate that the secondary metabolites obtained from crude extract of Aspergillus sp. present useful biological potential against vectors of public health importance and antibiotic-resistant bacteria.

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Secondary Metabolites with α-Glucosidase Inhibitory Activity from Mangrove Endophytic Fungus Talaromyces sp. CY-3

Marine Drugs ◽

10.3390/md19090492 ◽

2021 ◽

Vol 19 (9) ◽

pp. 492

Author(s):

Wencong Yang ◽

Qi Tan ◽

Yihao Yin ◽

Yan Chen ◽

Yi Zhang ◽

...

Keyword(s):

Secondary Metabolites ◽

13C Nmr ◽

Inhibitory Activity ◽

Endophytic Fungus ◽

Spectroscopic Data ◽

Positive Control ◽

Mangrove Endophytic Fungus ◽

Chemical Hydrolysis ◽

Ic50 Value ◽

New Compounds

Eight new compounds, including two sambutoxin derivatives (1–2), two highly oxygenated cyclopentenones (7–8), four highly oxygenated cyclohexenones (9–12), together with four known sambutoxin derivatives (3–6), were isolated from semimangrove endophytic fungus Talaromyces sp. CY-3, under the guidance of molecular networking. The structures of new isolates were elucidated by analysis of detailed spectroscopic data, ECD spectra, chemical hydrolysis, 13C NMR calculation, and DP4+ analysis. In bioassays, compounds 1–5 displayed better α-glucosidase inhibitory activity than the positive control 1-deoxynojirimycin (IC50 = 80.8 ± 0.3 μM), and the IC50 value was in the range of 12.6 ± 0.9 to 57.3 ± 1.3 μM.

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Phomosines H–J, Novel Highly Substituted Biaryl Ethers, Isolated from the Endophytic Fungus Phomopsis sp. from Ligustrum vulgare

Natural Product Communications ◽

10.1177/1934578x1100601229 ◽

2011 ◽

Vol 6 (12) ◽

pp. 1934578X1100601 ◽

Cited By ~ 3

Author(s):

Karsten Krohn ◽

Umar Farooq ◽

Hidayat Hussain ◽

Ishtiaq Ahmed ◽

Joachim Rheinheimer ◽

...

Keyword(s):

Mass Spectrometry ◽

Data Analysis ◽

Endophytic Fungus ◽

Spectroscopic Data ◽

Biologically Active ◽

The Novel ◽

Ligustrum Vulgare ◽

New Compounds

From the endophytic fungus Phomosis sp., four known phomosines A-D (1-4) and three new phomosines H-J (5-7) have been isolated. The structures of the new compounds were determined on the basis of their spectroscopic data analysis (1H, 13C, 1H-1H COSY, HMQC, and HMBC NMR, as well as mass spectrometry). The structures of phomosine H (5) and J (7) were also confirmed by semisynthesis from phomosine A (1). The remaining four known compounds [phomosines A-D (1-4)] were identified by comparing their spectroscopic data with those reported in the literature. The four known metabolites were biologically active. Of the novel metabolites, only 6 was antifungal and antibacterial.

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Kojic Acid Derivatives and Sesquiterpenes from the Aspergillus flavus GZWMJZ-288, A Fungal Endophyte of Garcinia multiflora

Natural Product Communications ◽

10.1177/1934578x1801301102 ◽

2018 ◽

Vol 13 (11) ◽

pp. 1934578X1801301 ◽

Cited By ~ 1

Author(s):

Yanchao Xu ◽

Liping Wang ◽

Qianyu Gong ◽

Guoliang Zhu ◽

Chunmao Yuan ◽

...

Keyword(s):

Aspergillus Flavus ◽

Endophytic Fungus ◽

Spectroscopic Data ◽

Kojic Acid ◽

Fungal Endophyte ◽

Solid Culture ◽

First Report ◽

Naturally Occurring ◽

New Compounds

One new kojic acid dimer, dikojiacid A (1), one new naturally occurring kojic acid dimer, dikojiacid B (2), one new sesquiterpene, 12-hydroxy-8-ene-3-oxodrimenol (6), as well as three known kojic acid derivatives (3–5) and five sesquiterpenes (7–11) were isolated from a solid culture of the endophytic fungus Aspergillus flavus GZWMJZ-288 with Garcinia multiflora. The structures of the new compounds were determined on the basis of spectroscopic data and ECD calculation. This is the first report for the natural kojic acid dimers by which Aspergillus flavus GZWMJZ-288 avoids the injury from acetaldehyde.

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Green Synthesis of Silver Nanoparticles from Ocimum sanctum against Mosquito Vectors for Malaria and Dengue

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2014.7.4.8 ◽

2014 ◽

Vol 7 (4) ◽

pp. 2650-2665

Author(s):

M. Syed Ali ◽

A.M. Aswan Ali ◽

Vijaya P P ◽

Yogananth N ◽

M. Munees Prabu

Keyword(s):

Silver Nanoparticles ◽

Aedes Aegypti ◽

Green Synthesis ◽

Larvicidal Activity ◽

Anopheles Stephensi ◽

Larval Mortality ◽

Probit Analysis ◽

Sem Analysis ◽

Fourth Instar ◽

Ocimum Sanctum

Vector-borne diseases such as malaria, filariasis, yellow fever, dengue, and Japanese encephalitis are major illnesses in in tropical countries. The main objective of this study was to investigate the larvicidal activity of synthesized silver nanoparticles (AgNPs) utilizing aqueous leaf extract of Ocimum sanctum against fourth instar larvae of Aedes aegypti and Anopheles stephensi. The present study was carried out to establish the larvicidal activity of synthesized silver nanoparticles (AgNPs) using leaf aqueous extract of Ocimum sanctum against fourth instar larvae of dengue and malaria vector. The larval mortality was observed after different time of exposures. Further, characterization such as XRD and SEM analysis were carried out for the synthesized silver nanoparticles. The mortality values were obtained using the probit analysis. The larvae of Aedes aegypti and Anopheles stephensi were found to be highly susceptible for the silver nanoparticles. The larvae have shown 100% mortality against the silver nanoparticles, whereas fourth instar larvae have shown efficacy (LC50) after 24 and 48 hr. The characterization studies of synthesized AgNPs by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), of silver nanoparticles along with stability. In conclusions, the findings revealed that synthesized AgNPs possess excellent mosquito larvicidal activity. These results suggest that the green synthesis of AgNPs have the potential to be used as an ideal eco-friendly approach for the control of head lice and vectors.

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