scholarly journals Physicochemical Properties and Bioactivity of a New Guar Gum-Based Film Incorporated with Citral to Brown Planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2044
Author(s):  
Xiubing Gao ◽  
Can Guo ◽  
Ming Li ◽  
Rongyu Li ◽  
Xiaomao Wu ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Guar Gum ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Transmission Electron ◽  
Lower Solubility ◽  
Film Forming ◽  
Significant Difference ◽  
Nilaparvata Lugens Stål

The brown planthopper (BPH), Nilaparvata lugens (Stål), is the most notorious rice insect pest. In order to repel BPH effectively while being environmentally friendly, a new film based on guar gum incorporated with citral (GC film) was formulated. A toxicity bioassay of citral and guar gum at different proportions (ratios of 3:1, 2:1, 1:1, 1:2, and 1:3 in w/w) of GC film-forming emulsion to BPH was performed with the rice stem dipping method. Results showed that the most effective ratio of citral to guar gum was 1:1 with the median lethal concentration (LC50) of 4.30 mg/mL, far below the LC50 of guar gum (GG)/citral individual (141.51 and 44.38 mg/mL, respectively). The mortality of BPH adults and nymphs in the third instar treated with different dilution multiples of GC film-forming emulsion ranged from 46.67% to 82.22% and from 37.78% to 71.11%, respectively. These indicated that GC film-forming emulsion had a direct toxicity on BPH, and the mixture of citral and GG had synergistic interactions. Subsequently, Fourier-transform infrared spectroscopy showed that the incorporation of guar gum with citral was successful and did not result in the formation of new chemical bonds. The GC film exhibited a darker color and rougher surface topography with larger apertures and deeper gullies (Ra = 1.42 nm, Rq = 2.05 nm, and Rmax = 25.40 nm) compared to the guar gum film (GG film) (Ra = 1.00 nm, Rq = 1.33 nm, and Rmax = 16.40 nm), as determined by transmission electron microscopy and atomic force microscopy. The GC film exhibited a 50.4% lower solubility in water (30.30% vs. 15.00%) and 71.3% oxygen permeability (8.26 × 10−9 vs. 2.37 × 10−9 cm3/m2·d·Pa) (p < 0.05) but did not demonstrate any significant difference in mechanical properties, such as thickness (39.10 vs. 41.70 mm), tensile strength (41.89 vs. 38.30 N/mm2), and elongation at break (1.82% vs. 2.03%) (p < 0.05) compared to the GG film. Our findings established a link between physicochemical properties and bioactivity, which can provide useful information on developing and improving GC films and may offer an alternative approach for the control of BPH in the near future.

scholarly journals Neutral Ceramidase Is Required for the Reproduction of Brown Planthopper, Nilaparvata lugens (Stål)

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao-Xiao Shi ◽  
Mu-Fei Zhu ◽  
Ni Wang ◽  
Yuan-Jie Huang ◽  
Min-Jing Zhang ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Hatching Rate ◽  
Female Reproduction ◽  
Egg Hatching ◽  
Tem Analysis ◽  
Transmission Electron ◽  
Nilaparvata Lugens Stål ◽  
Insect Reproduction

Ceramides are bioactive sphingolipids that have been implicated in insect development; however, their role in insect reproduction remains poorly understood. Here, we report the pivotal role of neutral ceramidase (NCER) in the female reproduction of the brown planthopper (BPH), Nilaparvata lugens (Stål), a significant pest in rice cultivation in Asia. LC-MS/MS demonstrated that, among different developmental stages of BPH, the levels of ceramides were highest in 1st instar nymphs and lowest in adults. The transcription of NCER was negatively correlated with the levels of ceramides at different developmental stages of BPH, in that the transcript levels of NCER were the highest, whereas ceramides levels were the lowest in BPH adults. Knocking down NCER through RNA interference (RNAi) increased the levels of ceramides in BPH females and ovaries, which resulted in a delay in oocyte maturation, a reduction in oviposition and egg hatching rate, as well as the production of vulnerable offspring. Transmission electron microscopy (TEM) analysis and TdT-mediated dUTP Nick-End Labeling (TUNEL) assays showed mitochondrial deficiency and apoptosis in NCER-deficient oocytes. Taken together, these results suggest that NCER plays a crucial role in female reproduction in BPH, likely by regulating the levels of ceramides.

scholarly journals Effect of variety and planting date of rice on population of natural enemies of brown planthopper, Nilaparvata lugens (Stal)

2014 ◽  
Vol 6 (2) ◽  
pp. 409-415 ◽  
Author(s):  
A. S. Tetarwal ◽  
Lakhi Ram ◽  
Ram Singh ◽  
Manoj Kumar Jat
Keyword(s):  
Natural Enemies ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Planting Date ◽  
Reverse Trend ◽  
Significant Difference ◽  
The Mean ◽  
Nilaparvata Lugens Stål

The present study on the effect of variety and planting date of rice on population of natural enemies of brown planthopper, Nilaparvata lugens (Stal) showed that during August, the mean population of spiders was statistically on par on CSR 30 and PR 114. The population differed with dates and was significantly (p=0.05) higher (9.0/10 hills) in D2 during 2011 only. There was no significant difference in the population of spiders on two varieties and dates of transplanting during September 2011 only. However, significantly higher population of spiders was recorded in variety PR 114 (21.65/10 hills) and D2 (20.52/10 hills) than on CSR 30 (13.67/10 hills) and D1 (14.80/10 hills) during September, 2012. The mean population of spiders did not differ significantly with the dates during October, 2011 and 2012. However, it was significantly higher on variety PR 114 (27.65/10 hills) than on CSR 30 during both the crop seasons. Mirid bugs did not appear in the month of August, 2011 and 2012 whereas during September 2011, the variety CSR 30 and D1 registered significantly higher population than other variety and date. However, a reverse trend was observed with varieties and dates during 2012. The mean population of coccinelid, carabid and staphylinid beetles remained very low on both the varieties in the month of August during 2011 and 2012. However, the population of these predators was influenced significantly by the varieties and dates during 2012. The population of nymphal-adult parasitoids also remained very low during both years and was not influenced by varieties and dates.

scholarly journals Uji Ekstrak Daun Tithonia diversifolia sebagai Penghambat Daya Makan Nilaparvata lugens Stal. pada Oryza sativa L. (Evaluation of Tithonia diversifolia Leaf Extract as Feeding Capacity Inhibitor of Nilaparvata lugens in Oryza sativa L.)

2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Tri A. Mokodompit ◽  
Roni Koneri ◽  
Parluhutan Siahaan ◽  
Agustina M Tangapo
Keyword(s):  
Oryza Sativa ◽  
Leaf Extract ◽  
Oryza Sativa L ◽  
Feeding Activity ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Tithonia Diversifolia ◽  
Feeding Inhibition ◽  
Nilaparvata Lugens Stål

AbstrakWereng Batang Coklat (WBC) (Nilaparvata lugens Stal.) merupakan serangga hama yang dapat merusak tanaman padi (Oryza sativa L.). Tanaman kipait (Tithonia diversifolia) berpotensi sebagai insektisida nabati karena memiliki senyawa toksik terhadap serangga. Penelitian ini bertujuan untuk mengkaji aktifitas makan akibat pemberian ekstrak daun kipait. Konsentrasi ekstrak daun kipait yang digunakan adalah 0% (kontrol), 1%, 3%,5% dan 7% dalam RAL (Rancangan Acak Lengkap) dengan 5 kali ulangan. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun kipait berpengaruh terhadap penghambatan daya makan WBC. Penghambatan makan tertinggi terjadi pada konsentrasi 7% setelah 24 jam.Kata kunci : penghambatan daya makan, Nilaparvata lugens Stal., Tithonia diversifoliaAbstractBrown planthopper (BPH) (Nilaparvata lugens Stal.) Is an insect pest that can damage rice plants (Oryza sativa L.). Kipait (Tithonia diversifolia) is potential as a bioinsecticide because it is toxic to insects. This study aimed to evaluate the feeding activity that was influenced by kipait leaf extract. The concentration of kipait leaf extract were 0% (control), 1%, 3%, 5% and 7%. The experiment design was CRD (completely randomized design) with 5 replications. The results showed that the kipait leaf extract influenced the feeding inhibition of BHP. The highest inhibition occurred in the concentration of 7% after 24 hours treatment.Keywords : brown planthopper, feeding inhibition, Tithonia diversifolia

scholarly journals Keragaan Sumber Kitin untuk Mempertahankan Virulensi Beauveria bassiana (Bals.), Jamur Pengendali Wereng Batang Cokelat (Nilaparvata lugens Stal.)

2018 ◽  
Vol 22 (2) ◽  
pp. 143
Author(s):  
Nova Laili Wisuda ◽  
Subur Sedjati
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Potato Dextrose Agar ◽  
Spore Density ◽  
Colony Forming Unit ◽  
Randomized Design ◽  
Significant Difference ◽  
Lethal Time ◽  
Rice Medium ◽  
Nilaparvata Lugens Stål

Beauvaria bassiana is an entomopathogenic insect that effectively controls brown planthopper (BPH), but will decrease it virulences if nutrients containing chitin and protein is not added. The aim of this research is to find the best method of B. bassiana propagation with the addition of shrimp and cricket flour, both are chitin sources. Isolates were cultured with Potato Dextrose Agar (PDA) medium and continued with rice medium. This research method used non-factorial Completely Randomized Design (CRD) with three treatments consisting of control, addition of shrimp flour and addition of cricket flour per treatment each with six replications. The research parameters consist of percentage of mortality, percentage of  conidia rising time, and LT50 test. The chitin sources could increases the growth rate from 0.69 cm per day to 1.449 cm per day and increases the spore density by 4.8×106 CFU (Colony Forming Unit) up to 8.2×106 CFU. Chitin also affects the virulence of B. bassiana in BPH where it can increase the percentage of death starting from 3%until10% and made lethal-time of BPH faster from 0.25 to 0.45 day. There is no significant difference between the sources of chitin between shrimp and cricket flour, so it is more advisable to use cricket flour because it is less expensive. IntisariBeauvaria bassiana merupakan serangga entomopatogen yang efektif mengendalikan wereng batang cokelat (WBC), namun akan mengalami penurunan virulensi bila tidak diberikan nutrisi yang mengandung khitin dan protein. Penelitian ini bertujuan menemukan metode terbaik perbanyakan B. bassiana yang dilakukan dengan penambahan tepung ebi dan penambahan tepung jangkrik yang merupakan sumber kitin. Isolat dibiakkan dengan medium per- banyakan Potato Dextrose Agar (PDA) dan diteruskan hingga perbanyakan beras. Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non-faktorial dengan 4 perlakuan dan 6 ulangan yang terdiri atas kontrol, tanpa tambahan kitin, penambahan tepung ebi dan penambahan tepung jangkrik. Parameter penelitian terdiri persentase mortalitas, persentase waktu munculnya konidia dan uji LT50. Pemberian sumber kitin meningkatkan kecepatan pertumbuhan hingga 0,69−1,49 cm per hari dan meningkatkan kerapatan spora berkisar 4,8×106−8,2×106 CFU (colony forming unit). Kitin juga berpengaruh terhadap virulensi B. bassiana pada WBC dimana mampu meningkatkan persentase kematian 3−10% dan waktu paruh kematian WBC 0,25−0,45 hari lebih cepat. Tidak ada perbedaan yang berarti antara sumber khitin dari tepung jangkrik dan tepung ebi sehingga lebih disarankan untuk menggunakan tepung jangkrik karena lebih murah dan mudah didapat.

scholarly journals Effect of Nutrient Management on Population Growth of Brown Planthopper, Nilaparvata lugens (Stål)

2014 ◽  
Vol 17 (1-2) ◽  
pp. 38-48
Author(s):  
MM Rashid ◽  
M Jahan ◽  
KS Islam ◽  
MN Bari ◽  
SS Haque
Keyword(s):  
Population Growth ◽  
Soil Nitrogen ◽  
Nutrient Management ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Dry Weight ◽  
Nilaparvata Lugens ◽  
Phosphorus Fertilization ◽  
High Potassium ◽  
Nilaparvata Lugens Stål

Brown planthopper (BPH), Nilaparvata lugens (Stål) is a major insect pest of rice in Bangladesh. Nutrient management in rice received little attention on BPH population. The present study evaluated the consequences of nitrogen (N), phosphorus (P) and potassium (K) application on population build up and weight of BPH in the laboratory and net-house at Entomology Division, Bangladesh Rice Research Institute. Three levels of soil nitrogen (0, 100, 200 kg N ha-1), phosphorus (0, 20, 40 kg P2O5 ha-1) and potassium (0, 60, 120 kg K2O ha-1) were compared for individual and combined effects. Interactions of N, P and K demonstrated no significant effect on population growth and weight of BPH. However, interaction of N and P showed significant effect on population growth of BPH. Fertilization with nitrogen increased population and dry weight of BPH. Phosphorus fertilization markedly increased the population growth while high potassium application decreased population build up and dry weight of BPH.DOI: http://dx.doi.org/10.3329/brj.v17i1-2.20900Bangladesh Rice j. 2013, 17(1&2): 38-48

scholarly journals Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata lugens (Hemiptera: Delphacidae)

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jinghua Zhu ◽  
Xiaoqing Liu ◽  
Kunmiao Zhu ◽  
Hanyu Zhou ◽  
Liang Li ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Target Genes ◽  
Transient Receptor Potential ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Survival Rates ◽  
Nilaparvata Lugens ◽  
Leg Extension ◽  
Significant Difference ◽  
Plant Sap

Abstract The vanilloid-type transient receptor potential (TRPV) channel is reported to be the molecular target of the commercial insecticide pymetrozine, which specifically disrupts the feeding of plant sap-sucking insects. However, the functions of TRPV channels in plant sap-sucking insects have not been fully elucidated. In the present study, RNA interference was used to investigate the effects of the knockdown of TRPV genes (Nan and Iav) on the mortality, locomotion, and feeding behavior of an important plant-feeding insect pest in rice, the brown planthopper, Nilaparvata lugens. Injecting dsRNA of Nan and Iav into fourth-instar nymphs significantly knocked down the target genes. The injection of dsNan or dsIav did not affect any morphological phenotype (including leg extension) of N. lugens nymphs and adults. Knockdown of Nan or Iav resulted in significantly decreased climbing activity against top plants but did not influence the leg-griping strength of adults. Knockdown of Nan resulted in a significantly elevated mortality of N. lugens in the observation period of 7 d after injection, whereas no significant difference in survival rates 7 d after injection was found between dsIav-injected and dsGFP-injected insects. Electropenetrographic (EPG) recordings indicated that knockdown of Nan and Iav reduced the ingestion activity in the rice phloem tissues of N. lugens. Knockdown of Nan and Iav significantly reduced the amount of honeydew excreted by N. lugens. Our findings indicated a relationship between TRPV and N. lugens locomotion and feeding behavior, which may help to fully elucidate the functions of TRPV in insects.

scholarly journals Conventional and Molecular Studies of Brown Planthopper (Nilaparvata lugens Stal) Resistance Genes in Rice: A Basis for Future Study of Natural Insect Resistance Genes Using Molecular Markers in Nepal

2015 ◽  
Vol 15 (1) ◽  
pp. 145-156 ◽  
Author(s):  
Prem N. Sharma ◽  
Naoki Mori ◽  
Shigeo Takumi ◽  
Chiharu Nakamura
Keyword(s):  
Molecular Markers ◽  
Insect Resistance ◽  
Resistance Genes ◽  
Insect Pests ◽  
Insect Pest ◽  
Durable Resistance ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Monogenic Resistance ◽  
Nilaparvata Lugens Stål

Rice productivity is greatly affected by various biotic and abiotic stresses. Insect-pests are one of the major bioticconstraints to cause significant losses in rice production. Brown planthopper (BPH), Nilaparvata lugens Stål, isthe most serious insect-pest of rice in Asia where most of the world rice is produced. Controlling insects usingchemicals is already proven detrimental not only to environment but also to human health. Integrated PestManagement (IPM) is the best approach to control insect pests. Host plant resistance is the principal componentof IPM along with biological, cultural and physical methods. Use of varietal resistance is the best option to controlBPH. Many BPH resistant rice varieties with natural BPH resistance have been developed and widely used againstBPH. However, frequent breakdown of monogenic resistance by new BPH biotypes has been a serious threat tocontrol BPH. To overcome such difficulty in the use of monogenic resistance, development of durable resistanceis needed as the sustainable means to control BPH. To develop durable resistance, pyramiding of BPH resistancegenes and quantitative trait loci (QTLs), through marker-assisted method, is needed. For this, many BPH resistancegenes and QTLs have already been identified and mapped on rice chromosomes. This article reviews identification,mapping and pyramiding toward successful cloning of BPH resistance genes/QTLs and provides the basis/guidelines to work on natural insect resistance genes using molecular markers in Nepal.DOI: http://dx.doi.org/10.3126/njst.v15i1.12032Nepal Journal of Science and TechnologyVol. 15, No.1 (2014) 145-156

scholarly journals Resistensi Wereng Batang Cokelat Padi, Nilaparvata lugens Stål terhadap Insektisida di Indonesia

2016 ◽  
Vol 10 (3) ◽  
pp. 115
Author(s):  
Sutrisno Sutrisno
Keyword(s):  
Insecticide Resistance ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Resistance Management ◽  
Nilaparvata Lugens ◽  
Insecticide Resistance Management ◽  
Mechanism Of Resistance ◽  
Development Of Resistance ◽  
Resistance Inheritance ◽  
Nilaparvata Lugens Stål

<p>The rice<br />brown planthopper (BPH), Nilaparvata lugens (Stål) is a<br />major insect pest of rice and their infestations occur every<br />year in several locations in Indonesia. The use of<br />insecticides often fails to control the BPH so their<br />populations are still high that cause rice crops show<br />hopperburn and the farmer loses the yields. The<br />development of insecticide resistant in BPH population is<br />one of the factors to contribute to the failure of insecticides<br />control. We have detected the development of field<br />population BPH resistance to BPMC, carbofuran, MIPC, and<br />imidacloprid, but we do not know yet the development of<br />resistance to other insecticides to control BPH in Indonesia.<br />This paper will review several cases on BPH resistance to<br />insecticides in Indonesia and other countries that include<br />aspects of the development of resistance in the field and in<br />the laboratory, the mechanism of resistance, inheritance of<br />resistance, genomics of resistance, and resistance<br />management. A policy and further study is also suggested for<br />insecticide resistance management in Indonesia.</p>

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