scholarly journals Optimization of Multiparameters for Increased Yields of Cytochrome B5 in Bioreactors

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4148
Author(s):  
Ricardo F. S. Pereira ◽  
Carla C. C. R. de Carvalho
Keyword(s):  
Recombinant Proteins ◽  
Process Parameters ◽  
Pid Controller ◽  
Biomass Yield ◽  
Culture Media ◽  
Cytochrome B5 ◽  
Aeration Rate ◽  
Diameter Ratio ◽  
Recombinant Cells ◽  
Fine Tune

The production of recombinant proteins is gaining increasing importance as the market requests high quality proteins for several applications. However, several process parameters affect both the growth of cells and product yields. This study uses high throughput systems and statistical methods to assess the influence of fermentation conditions in lab-scale bioreactors. Using this methodology, it was possible to find the best conditions to produce cytochrome b5 with recombinant cells of Escherichia coli. Using partial least squares, the height-to-diameter ratio of the bioreactor, aeration rate, and PID controller parameters were found to contribute significantly to the final biomass and cytochrome concentrations. Hence, we could use this information to fine-tune the process parameters, which increased cytochrome production and yield several-fold. Using aeration of 1 vvm, a bioreactor with a height-to-ratio of 2.4 and tuned PID parameters, a production of 72.72 mg/L of cytochrome b5 in the culture media, and a maximum of product to biomass yield of 24.97 mg/g could be achieved.

scholarly journals Optimizing of Microalgae Scenedesmus sp. Biomass Production in Wet Market Wastewater Using Response Surface Methodology

2021 ◽  
Vol 13 (4) ◽  
pp. 2216
Author(s):  
Najeeha Mohd Apandi ◽  
Mimi Suliza Muhamad ◽  
Radin Maya Saphira Radin Mohamed ◽  
Norshuhaila Mohamed Sunar ◽  
Adel Al-Gheethi ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Yield ◽  
Biomass Productivity ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Aeration Rate ◽  
Composition Analysis ◽  
Wet Market ◽  
Central Composite

The present study aimed to optimize the production of Scenedesmus sp. biomass during the phycoremediation process. The biomass productivity was optimized using face centred central composite design (FCCCD) in response surface methodology (RSM) as a function of two independent variables that included wet market wastewater concentrations (A) with a range of 10% to 75% and aeration rate (B) with a range of 0.02 to 4.0 L/min. The results revealed that the highest biomass productivity (73 mg/L/d) and maximum growth rate (1.19 day−1) was achieved with the 64.26% of (A) and 3.08 L/min of (B). The GC-MS composition analysis of the biomass yield extract revealed that the major compounds are hexadecane (25%), glaucine (16.2%), and phytol (8.33%). The presence of these compounds suggests that WMW has the potential to be used as a production medium for Scenedesmus sp. Biomass, which has several applications in the pharmaceutical and chemical industry.

scholarly journals Deleting multiple lytic genes enhances biomass yield and production of recombinant proteins by

2014 ◽  
Vol 13 (1) ◽  
pp. 129 ◽  
Author(s):  
Yi Wang ◽  
Zhenmin Chen ◽  
Ruili Zhao ◽  
Tingting Jin ◽  
Xiaoming Zhang ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Biomass Yield ◽  
Lytic Genes

Numerical Simulation on Upsetting Process of Large Forgings

2013 ◽  
Vol 423-426 ◽  
pp. 931-934
Author(s):  
Jing Meng ◽  
Qiang Wang ◽  
Dong Mei Cai
Keyword(s):  
Numerical Simulation ◽  
Tangential Stress ◽  
Process Parameters ◽  
Diameter Ratio ◽  
Fem Model ◽  
Theoretical Methods ◽  
Shape Coefficient ◽  
Change Tendency

The numerical simulation on upsetting process of large forgings is conducted by using ANSYS/LS-DYNA. The FEM model is established by determining materials, contacts, constraints and loads. The distributions of strain and stress are calculated and discussed. Drum shape coefficient is defined to measure the nonuniform in deformation. The simulative values of upsetting forces are compared with those in theoretical methods, which gives the same change tendency and consistency. Influences of height-diameter ratio to strain, drum shape, tangential stress and upsetting force are also investigated, which provides basic guidance to determine process parameters in upsetting.

scholarly journals Preliminary Investigation of Naringenin Hydroxylation with Recombinant E. coli Expressing Plant Flavonoid Hydroxylation Gene

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500
Author(s):  
Ilef Limem-Ben Amor ◽  
Nidhal Salem ◽  
Emmanuel Guedon ◽  
Jean-Marc Engasser ◽  
Leila Chekir-Ghedrira ◽  
...  
Keyword(s):  
Biomass Yield ◽  
Culture Media ◽  
Plasmid Stability ◽  
Biological Activities ◽  
Preliminary Investigation ◽  
Petroselinum Crispum ◽  
Growth Media ◽  
Hydroxyl Groups ◽  
E Coli ◽  
Cloned Gene

Flavonoid hydroxylation is one way to increase the biological activities of these molecules and the number of hydroxyl groups needed for polymerization, esterification, alkylation, glycosylation and acylation reactions. These reactions have been suggested as a promising route to enhance flavonoid solubility and stability. In our preliminary study we hydroxylated naringenin (the first flavonoid core synthesized in plants) with recombinant E. coli harboring flavanone 3 hydroxylase (F3H). We demonstrated that recombinant E. coli harboring the F3H from Petroselinum crispum, can convert naringenin to dihydrokaempferol. The whole cell hydroxylase activity was often influenced by the stability of the plasmid harboring the cloned gene and the biomass yield. When the composition of the growth media became richer the amount of formed product decreased about twofold; the naringenin bioconversion yield in LB media was 70% and decreased to 33% in TB. However, the enrichment of culture media increased the biomass yield nearly threefold in LB media, only 0.5 g/L of bacteria was formed, but in TB there was 1.6 g/L. Thus, LB constitutes the best medium for naringenin bioconversion using the recombinant E. coli harboring the F3H; this allows for maximum bioconversion yield and plasmid stability when compared with the fourth tested culture medium. Consequently, E. coli harboring F3H from Petroselinum crispum can be used to produce flavonoids hydroxylated in position 3 that can serve in additional reactions like polymerization, glycosylation, and acylation,

scholarly journals Deleting multiple lytic genes enhances biomass yield and production of recombinant proteins by Bacillus subtilis

2014 ◽  
Vol 13 (1) ◽  
Author(s):  
Yi Wang ◽  
Zhenmin Chen ◽  
Ruili Zhao ◽  
Tingting Jin ◽  
Xiaoming Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Recombinant Proteins ◽  
Biomass Yield ◽  
Lytic Genes

scholarly journals Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk

2019 ◽  
Vol 21 (1) ◽  
pp. 82-90
Author(s):  
Valentina Palacio-Castañeda ◽  
Alejandra Pérez-Hoyos ◽  
Daniel Carrascal-Correa ◽  
Victor Manuel Osorio-Echeverri
Keyword(s):  
Staphylococcus Aureus ◽  
Culture Media ◽  
Human Cancer ◽  
Aeration Rate ◽  
Pigment Production ◽  
Stirred Tank ◽  
Human Cancer Cell ◽  
Stirred Tank Bioreactor ◽  
Human Cancer Cell Lines ◽  
Yield Production

Prodigiosin,  a  pigment  produced  by Serratia  marcescensinhibits  the  growth  of  different  microorganisms  and  the  proliferation  of some human cancer cell lines. Prodigiosin is usually produced by fermentations of substrates such as starch and proteins, andpig-ment yield depends on the concentration of the carbon source, stirring speed of cultures, temperature and time of incubation,nitro-gen  sources,  and  pH  of  medium.  In  the  present  study,  pigment  production  was  assessed  using  two  types  of  casein  as  substrate; pigment yield was greater when casein precipitated with vinegar was used as substrate than when high purity casein was. The maxi-mum prodigiosin production was achieved with 10 g/L casein at pH 8.0. Additionally, supplementation of culture media with glu-cose was found to considerably decrease prodigiosin production and growth inhibition of Staphylococcus aureus, which is directly related to pigment yield. Production in stirred-tank bioreactor at 0.75 vvm aeration was higher than that at 0.5 and 1.0 vvm. Sub-strate type, concentration and pH affected pigment production in Erlenmeyer flasks, whereas aeration rate influenced pigment pro-duction in a stirred-tank bioreactor.

scholarly journals Physiology and lipid accumulation capacity of differentYarrowia lipolyticaandRhodosporidium toruloidesstrains on glycerol

2018 ◽  
Author(s):  
Suellen Patricia Held Azambuja ◽  
Nemailla Bonturi ◽  
Everson Alves Miranda ◽  
Andreas Karoly Gombert
Keyword(s):  
Process Parameters ◽  
Lipid Accumulation ◽  
Consumption Rate ◽  
Biomass Yield ◽  
Dry Weight ◽  
Exponential Growth Phase ◽  
Specific Substrate ◽  
Significant Difference ◽  
Accumulation Capacity ◽  
Main Carbon

AbstractObjectiveTo compare physiological and process parameters, as well as lipid accumulation capacity, of six strains ofYarrowia lipolyticaand two strains ofRhodosporidium toruloidesin media containing glycerol as the main carbon and energy source.ResultsThe strainsY. lipolyticaIMUFRJ 50678, Po1g, W29 and CCT 5443 displayed very similar physiological parameters, with µmax,andaverage values of 0.31 h−1, 0.53 (g DW/g S), 0.60 (g S/g DW.h) and 0.46 (g DW/g S), respectively. The two strains ofR. toruloidespresented physiological and process parameters with no significant difference, with average values of 0.084 h−1, 0.53 (g DW/g S), 0.17 (g S/g DW.h) and 0.44 (g DW/g S). Among allY. lipolyticastrains,Y. lipolyticaCCT 5443 strain presented the highestYLip/S, 0.054 (g Lip/g S), andPLipof 0.040 (g Lip/l.h). Among all investigated strains (Y. lipolyticaandR. toruloides), the yeastR. toruloidesCCT 7815 displayed the highest lipid accumulation capacity, withYLip/Sequal to 0.11 (g Lip/g S) andPLipequal to 0.10 (g Lip/l.h).ConclusionAmong all strains investigated in our study, the yeast strainR. toruloidesCCT 7815 presents the most promising characteristics for industrial single cell oil production.List of abbreviationsDWdry weight (g/l)Liplipid (g/l)PLiplipid productivity (g Lip/l.h)maximum specific substrate consumption rate (g S/g DW.h)Ssubstrate (g/l)YLip/Slipid yield on substrate (g Lip/g S)biomass yield on substrate during the exponential growth phase (g DW/g S)global biomass yield on substrate (g DW/g S)µmaxmaximum specific growth rate (h−1)

scholarly journals Assessing the salt tolerance of Spirulina platensis freshwater strains and examining cheap culture media for cultivation of the potential strain

2021 ◽  
Vol 19 (2) ◽  
pp. 381-392
Author(s):  
Luu Thi Tam ◽  
Le Thi Thom ◽  
Nguyen Cam Ha ◽  
Hoang Thi Minh Hien ◽  
Ngo Thi Hoai Thu ◽  
...  
Keyword(s):  
Crude Protein ◽  
Functional Foods ◽  
Culture Medium ◽  
Biomass Yield ◽  
Specific Growth ◽  
Culture Media ◽  
Statistical Significance ◽  
Scientific Basis ◽  
Natural Seawater ◽  
Marine And Brackish Waters

Spirulina cyanobacteria have been widely cultivated to exploit products such as crude protein, vitamins, phycocyanin pigment... with high nutritional and pharmacological values. However, the commercialization of these products is still a challenging issue due to high biomass cost, which is mainly caused by expensive nutrients in the culture medium. In this study, from 11 freshwater S. platensis strains, by culture screening, we found 7 strains being capable of profitable growth on inexpensive seawater with salinity ranging from 5 - 30‰, and selected ST strain as the potential strain for further study. Natural seawater must be pretreated to remove ions that easily cause precipitation of nutrients in the culture medium such as Mg2+, Ca2+, SO42-… before using. The ST strain showed the best growth in the natural seawater medium with 30‰ salinity containing 3 g/L NaNO3, 0.5 g/L K2HPO4, 0.05 g/L FeSO4. This strain reached the highest biomass yield at 0.487 g/L and the specific growth rate (µ) of 0.12 x day-1; protein and phycocyanin contents reached 48.6% and 127 mg/g of dry biomass, respectively. There was no difference in the mentioned above values with biological statistical significance between this medium and SOT medium in distilled water. The ST strain biomass was qualified to be used for the production of functional foods. Results of this study provided scientific basis for the use of marine and brackish waters to produce biomass of this highly economic cyanobacterium.

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