scholarly journals Green Synthesis of Silver Nanoparticles Using Natural Extracts with Proven Antioxidant Activity

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4986
Author(s):  
Jolanta Flieger ◽  
Wojciech Franus ◽  
Rafał Panek ◽  
Monika Szymańska-Chargot ◽  
Wojciech Flieger ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Green Synthesis ◽  
Radical Scavenging ◽  
Porphyra Yezoensis ◽  
Antioxidant Power ◽  
Capsicum Baccatum ◽  
Natural Extracts ◽  
Diet Supplements ◽  
Dpph Method ◽  
Vis Spectroscopy

Natural extracts are a rich source of biomolecules that are useful not only as antioxidant drugs or diet supplements but also as complex reagents for the biogenic synthesis of metallic nanoparticles. The natural product components can act as strong reducing and capping substrates guaranteeing the stability of formed NPs. The current work demonstrates the suitability of extracts of Camellia sinensis, Ilex paraguariensis, Salvia officinalis, Tilia cordata, Levisticum officinale, Aegopodium podagraria, Urtica dioica, Capsicum baccatum, Viscum album, and marine algae Porphyra Yezoensis for green synthesis of AgNPs. The antioxidant power of methanolic extracts was estimated at the beginning according to their free radical scavenging activity by the DPPH method and reducing power activity by CUPRAC and SNPAC (silver nanoparticle antioxidant capacity) assays. The results obtained by the CUPRAC and SNAPC methods exhibited excellent agreement (R2~0.9). The synthesized AgNPs were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), dynamic light scattering (DLS) particle size, and zeta potential. The UV-vis absorption spectra showed a peak at 423 nm confirming the presence of AgNPs. The shapes of extract-mediated AgNPs were mainly spherical, spheroid, rod-shaped, agglomerated crystalline structures. The NPs exhibited a high negative zeta potential value in the range from −49.8 mV to −56.1 mV, proving the existence of electrostatic stabilization. FTIR measurements indicated peaks corresponding to different functional groups such as carboxylic acids, alcohol, phenol, esters, ethers, aldehydes, alkanes, and proteins, which were involved in the synthesis and stabilization of AgNPs. Among the examined extracts, green tea showed the highest activity in all antioxidant tests and enabled the synthesis of the smallest nanoparticles, namely 62.51, 61.19, and 53.55 nm, depending on storage times of 30 min, 24 h, and 72 h, respectively. In turn, the Capsicum baccatum extract was distinguished by the lowest zeta potential, decreasing with storage time from −66.0 up to −88.6 mM.

scholarly journals Green Synthesis of Polyherbal Silver Nanoparticles from Rosa Gallia officinalis, Citrus sinensis and Solanum tuberosum Extract for antioxidant Potency

2020 ◽  
Vol 11 (4) ◽  
pp. 6975-6985
Author(s):  
Brito Raj S ◽  
Boukary Obedoulaye ◽  
Sucharitha P ◽  
Saritha M ◽  
Shaheedha S M ◽  
...  
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Green Synthesis ◽  
Chemical Reduction ◽  
Radical Scavenging ◽  
Entrapment Efficiency ◽  
The Body ◽  
Free Radical Damage ◽  
Ic50 Values ◽  
Antioxidant Potency

Skin ageing is due to the combination of natural, largely genetically programmed and environmentally modulated changes which occur in the body system due to free radical damage. Silver Nanoparticle (AgNPs), were prepared by chemical reduction using green synthesis and they were evaluated for particle size in nanometer, zeta potential in millivolt, surface morphology by scanning electron microscopy (SEM) and percent entrapment efficiency. The polyphenols were quantified by chromatographic techniques and the antioxidant activity measured spectrophotometrically by DPPH (2,2 Diphenyl 1 picrylhydrazyl) assay. According to this study AgNPs showed a least particle size of 145.4±2.4nm, maximum zeta potential of -39.1±2.4 mV with desired polydispersity index of 0.358±0.02, the amount of polyphenols loaded in AgNPs was found to be 87.23±2.54%. Maximum phenolic content was found in F1 as 65.21 ± 3.721 mg equivalent GAE/g of extract. On comparing the IC50  values, F1 and F5 exhibited the lowest and highest values respectively. Therefore, F1 possesses higher DPPH radical scavenging potential.

scholarly journals Antioxidant Activity of Spray-Dried Extracts of Psidium guajava Leaves

2018 ◽  
Vol 7 (4) ◽  
pp. 141 ◽  
Author(s):  
Maurette R. V. Fernandes ◽  
Luciana M. Kabeya ◽  
Cláudia R. F. Souza ◽  
Adna P. Massarioli ◽  
Severino M. Alencar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Psidium Guajava ◽  
Absorption Capacity ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Dpph Scavenging ◽  
Spray Dried

Antioxidants from Psidium guajava leaves were extracted with 70% ethanol in water. The extractive solution was concentrated and submitted to spray drying in the presence of technological adjuvants at a proportion of 8 % wet base. Three distinct technological adjuvants were evaluated: β-cyclodextrin (βCD-80), maltodextrin DE10:Aerosil® (MA-80 - 7:1), and maltodextrin DE10:Encapsia®:Aerosil® (MDEA-80 - 5:2:1). The antioxidant activity of the concentrated extract and spray-dried powders was assessed by three antioxidant assays, namely: the2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP), and oxygen radical absorption capacity (ORAC). The 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging capacity was determined previously. The spray-dried powders exhibited strong antioxidant activity (IC50 value = 7.96 to 9.76 μg/mL – DPPH method; 3,125.1 to 3,406.0 μM TE/g dry weight – ABTS method; 4,210 to 4,540 μM FeSO4 E/g – FRAP method; 1,820-2,020 μM TE/g – ORAC method). The technological adjuvants did not significantly interfere with the antioxidant activity of the dried products, regardless the type of antioxidant assay used. The results here reported strongly evidenced that the concentrated and spray-dried extracts of Psidium guajava are rich sources of natural antioxidants with potential application in food, pharmaceutical, and cosmeceutical products.

scholarly journals Green Synthesis of Gold Nanoparticles Using Upland Cress and Their Biochemical Characterization and Assessment

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 28
Author(s):  
Noah Hutchinson ◽  
Yuelin Wu ◽  
Yale Wang ◽  
Muskan Kanungo ◽  
Anna DeBruine ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Zeta Potential ◽  
Green Synthesis ◽  
Synthesis Process ◽  
Gold Content ◽  
Face Centered Cubic ◽  
X Ray Diffraction ◽  
Au Nps ◽  
X Ray ◽  
Vis Spectroscopy

This research focuses on the plant-mediated green synthesis process to produce gold nanoparticles (Au NPs) using upland cress (Barbarea verna), as various biomolecules within the upland cress act as both reducing and capping agents. The synthesized gold nanoparticles were thoroughly characterized using UV-vis spectroscopy, surface charge (zeta potential) analysis, scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDX), atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and X-ray diffraction (XRD). The results indicated the synthesized Au NPs are spherical and well-dispersed with an average diameter ~11 nm and a characteristic absorbance peak at ~529 nm. EDX results showed an 11.13% gold content. Colloidal Au NP stability was confirmed with a zeta potential (ζ) value of −36.8 mV. X-ray diffraction analysis verified the production of crystalline face-centered cubic gold. Moreover, the antimicrobial activity of the Au NPs was evaluated using Gram-negative Escherichiacoli and Gram-positive Bacillus megaterium. Results demonstrated concentration-dependent antimicrobial properties. Lastly, applications of the Au NPs in catalysis and biomedicine were evaluated. The catalytic activity of Au NPs was demonstrated through the conversion of 4-nitrophenol to 4-aminophenol which followed first-order kinetics. Cellular uptake and cytotoxicity were evaluated using both BMSCs (stem) and HeLa (cancer) cells and the results were cell type dependent. The synthesized Au NPs show great potential for various applications such as catalysis, pharmaceutics, and biomedicine.

scholarly journals Polyphenols Content and Antimicrobial, Antioxidant and Hemolytic Activities of Essential Oils from Four Selected Medicinal Plants Growing in Algeria

2020 ◽  
Vol 9 (2) ◽  
pp. 65-75
Author(s):  
Noureddine Halla ◽  
Kebir Boucherit ◽  
Bankaddour Zeragui ◽  
Abdelkader Djelti ◽  
Ziane Belkhedim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extreme Environment ◽  
Positive Control ◽  
Mentha Piperita ◽  
Antioxidant Power ◽  
Dpph Method

The Saharan and steppe spontaneous plants are very characteristic because of their particular adaptation to the desert and extreme environment. Some species have pharmacological properties that give them a medicinal interest. The aim of the present work was to determine the polyphenol contents of essential oils obtained from four endemic plants growing in Algeria (Pituranthos scoparius, Myrtus nivellei, Rosmarinus officinalis and Mentha piperita), and study its biological activity, including antimicrobial, antioxidant, and hemolytic. The antimicrobial activity was evaluated by the microdilution method against twelve strains. The antioxidant activity was carried out by two methods (DPPH radical scavenging and reducing power). However, the hemolytic effect has been evaluated against the red blood cells. P. scoparius and M. piperita showed yields of essential oils higher than 1%. All the strains showed sensitivity against the essential oils tested with the exception of the C. albicans treated by R. officinalis essential oils. The most sensitive strain was C. albicans treated by P. scoparius essential oils by MIC of 0.0781 mg/mL, it was the same plant that shows the highest polyphenol content (14.78 ± 0.72 g GAE/g DS). The antioxidant activity by the DPPH method was greater for all essential oils tested by IC50 ranging from 0.69 ± 0.07 (R. officinalis) to 30.67 ± 2.12 mg/mL (M. nivellei). The R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid). In reducing iron, it was the R. officinalis essential oils which were found to be the most active with an EC50 concentration of 9.67 ± 1.36 mg/mL. After 120 min incubation, minimal haemolysis (10%) was obtained with essential oils of R. officinalis at a concentration of 0.39 mg/mL. We conclude that P. scoparius essential oils showed the high content of polyphenols and R. officinalis essential oils reported more antioxidant power than the positive control (ascorbic acid).

scholarly journals Antioxidant Activity and Total Flavonoid of Carica papaya L. Leaves with Different Varieties, Maturity and Solvent

2019 ◽  
Vol 39 (1) ◽  
pp. 54 ◽  
Author(s):  
Fatma Zuhrotun Nisa ◽  
Mary Astuti ◽  
Sofia Mubarika Haryana ◽  
Agnes Murdiati
Keyword(s):  
Antioxidant Activity ◽  
Carica Papaya ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Water Extraction ◽  
Antioxidant Power ◽  
Mature Leaves ◽  
Dpph Method ◽  
Young Leaves ◽  
Ferric Reducing Antioxidant Power

Carica papaya leaves are one of the vegetables consumed by Indonesian people, especially in Java Island. Carica papaya is easy to grow in Indonesia and has many variants, so, Carica Papaya leaves is a local potent to be developed for functional food and nutraceutical. The aim of this study was to investigate antioxidant activity and total flavonoids of Carica papaya leaves with different varieties, maturity and solvent. Carica papaya leaves (CPL) was firstly extracted by methanol to select two CPLs with high antioxidant capacity and total flavonoid. The two selected CPLs were further tested with different ages mainly young and mature leaves. One selected CPL was further tested with different extraction solvents. Antioxidant activity was determined by 2.2 diphenyl-1-picrylhydrazyl, DPPH and Ferric reducing antioxidant power, FRAP. This study used five varieties of Carica papaya leaves, namely Bangkok, California, Purple, Golden and Grendel. The result showed that Golden and Grendel varieties had a higher percentage of radical scavenging property than the others, which was 78.37% and 77.40% by the DPPH method. Grendel and Purple had a higher percentage of radical scavenging property, which was 45.82 and 34.32 mmol/mg. Grendel and Purple had a higher total flavonoid property, which was 50.33 and 46.02 µg/g. Mature leaves had a higher percentage of radical scavenging property than young leaves by DPPH and FRAP methods. Mature leaves had a higher total flavonoid property than young leaves in both Grendel and Purple. Grendel had a higher antioxidant activity and a higher total flavonoid property than Purple. Grendel with water extraction had a higher antioxidant activity by DPPH and FRAP methods. The total flavonoid of Grendel papaya leaves’ extract with water extraction was lower than ethanol 70% and methanol.

scholarly journals Influence of Packaging Material on Polyphenol Content and Antioxidant Activity in Some Commercial Beers

2020 ◽  
Author(s):  
Mihaela Mirela Bratu ◽  
Semaghiul Birghila ◽  
Antoanela Popescu ◽  
Bogdan Stefan Negreanu- Pirjol ◽  
Marius Radu
Keyword(s):  
Antioxidant Activity ◽  
Plastic Material ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Packaging Material ◽  
Polyphenol Content ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

<div><div><div><div><p>Using two methods (ferric reducing antioxidant power and radical scavenging activity), the total polyphenol content (Folin–Ciocalteu reagent) and polyphenol patterns (HPLC) in 10 commercial lager beer brands produced in Romania was determined. Samples bottled in glass, plastic and aluminium packages were analysed for each brand when available. Results have indicated considerable variations in the total and individual phenolic contents as well as antioxidant activity across beer brands. A statistical analysis was performed to assess the influence of packaging type on the antioxidant activity and phenolic content of the beers. Statistical differences were found between the DPPH and FRAP methods in glass, aluminium and plastic material. Moreover, the antioxidant activity based on the DPPH method is influenced by the type of packaging material, while in the case of FRAP method, no statistical difference was reported. Furthermore, the same analysis has shown that the polyphenol concentration is invariant to the type of material.</p></div></div></div></div>

scholarly journals Influence of Packaging Material on Polyphenol Content and Antioxidant Activity in Some Commercial Beers

2020 ◽  
Author(s):  
Mihaela Mirela Bratu ◽  
Semaghiul Birghila ◽  
Antoanela Popescu ◽  
Bogdan Stefan Negreanu- Pirjol ◽  
Marius Radu
Keyword(s):  
Antioxidant Activity ◽  
Plastic Material ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Packaging Material ◽  
Polyphenol Content ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

<div><div><div><div><p>Using two methods (ferric reducing antioxidant power and radical scavenging activity), the total polyphenol content (Folin–Ciocalteu reagent) and polyphenol patterns (HPLC) in 10 commercial lager beer brands produced in Romania was determined. Samples bottled in glass, plastic and aluminium packages were analysed for each brand when available. Results have indicated considerable variations in the total and individual phenolic contents as well as antioxidant activity across beer brands. A statistical analysis was performed to assess the influence of packaging type on the antioxidant activity and phenolic content of the beers. Statistical differences were found between the DPPH and FRAP methods in glass, aluminium and plastic material. Moreover, the antioxidant activity based on the DPPH method is influenced by the type of packaging material, while in the case of FRAP method, no statistical difference was reported. Furthermore, the same analysis has shown that the polyphenol concentration is invariant to the type of material.</p></div></div></div></div>

scholarly journals ANTIOXIDANT ACTIVITY (PHENOL AND FLAVONOID CONTENT ) OF THREE DIFFERENT CULTIVARS OF PIPER BETLE L. (PIPERACEAE)

2018 ◽  
Vol 8 (5-s) ◽  
pp. 143-148
Author(s):  
SAPARA SEKHAR HARINI ◽  
Pagadala R Sougandhi ◽  
DR SHOBHA RANI TENKAYALA ◽  
Kamalamma Ramalingam Gopinath
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Piper Betle ◽  
Drug Preparation ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Dpph Method

In the present study, an attempt has been made for the estimation of total phenol and flavonoid content and their radical scavenging properties using Piper betle (L.) leaves. In that, Cultivars i.e., Nov Bangla (NB), Sirugamani-1 (SGM-1) and Halisar Sanchi (HS) were selected for this study. The total phenolic content was ranged from 95.04 to 127.33 mg/100g equivalent to gallic acid and flavonoids were ranged from 51.72 to 61.08 mg/ 100g equivalent to standards of Catechin.  In vitro antioxidant activity was estimated using 1,1-diphenyl-2-picryl hydrazyl (DPPH), free radical scavenging activity, improved ABTS radical cation decolorization assay and ferric reducing antioxidant power (FRAP) assay. Among all the cultivars, The highest Phenol content (93.79%) was observed  for Sirugamani-1 by DPPH method  and highest Phenol content (96.12% & 6791.86 (µg/g) was obtained  for Halisar Sanchi by ABTS assay and FRAP activity  respectively. The study revealed that the leaves of Piper betle (L.) has higher amount of antioxidant activity and it could be used for any novel drug preparation.

Green synthesis, Characterization and In vitro Biological Studies of Quercetin complexes with Zn (II) Acetate and N^N Moiety

2021 ◽  
pp. 3585-3590
Author(s):  
Tanu Srivastava ◽  
S. K. Mishra ◽  
O. P. Tiwari ◽  
Kavindra Nath Tiwari ◽  
Pradeep Kumar ◽  
...  
Keyword(s):  
Green Synthesis ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Coordination Complexes ◽  
E Coli ◽  
Biological Studies ◽  
Dpph Method ◽  
Ft Ir ◽  
High Yields

Green synthesis of two coordination complexes of zinc acetate with N^N moiety on quercetin, which is a flavonoid is carried out. The complexes were obtained in high yields (> 97%) by grinding methods without the involvement of any solvent. Neither catalyst nor any additives were needed to perform the reactions. It was characterized by FT-IR, UV-Vis, NMR, HRMS, and elemental analysis. Antioxidant activity was done through the DPPH method which was compared with ascorbic acid and ligand (Quercetin). The study reveals that Complex 1 (IC50 163.093µg/ml) has significant free radical scavenging activity as compared to complex 2 (IC50 258.683µg/ml). Biological activity was performed against microbes (E. coli and S. aureus). MIC value of complex 1 (15.50µg/ml E. coli, 7.18µg/ml S. aureus) was found more significant as compared to complex 2 (22.51µg/ml E. coli, 18.62µg/ml S. aureus) and quercetin.

scholarly journals BSA binding affinity, free radical scavenging capacity and antibacterial activity of new Silver(I) complexes with Schiff base ligands

2020 ◽  
Vol 9 (3) ◽  
pp. 12
Author(s):  
Emir Horozić ◽  
Maida Šljivić Husejnović ◽  
Adem Bajrić ◽  
Merima Ibišević ◽  
Enida Karić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Schiff Bases ◽  
Conformational Changes ◽  
Radical Scavenging ◽  
Bacterial Strains ◽  
Antimicrobial Screening ◽  
Dpph Method ◽  
Vis Spectroscopy ◽  
Ic50 Values

In this work, four silver(I) complexes with Schiff bases derived from ninhydrin and selected amino acids (methionine, histidine, cysteine, and phenylalanine) were synthesized. The aim of this study is to determine the potential biological activity of these complexes. FTIR and UV/VIS spectroscopy were used for structural characterization of the products. Antioxidant activity was examined in vitro using DPPH method. Antimicrobial screening was performed by diffusion technique on reference bacterial strains from the ATCC collection. Interaction with bovine serum albumin (BSA) was examined using UV/VIS spectroscopy. Based on the obtained spectral data, it is assumed that all Schiff bases coordinate the Ag(I) ion as a tridentate ONO donor ligand. The antioxidant activity of the synthesized compounds is extremely high, with a range of IC50 values of 0.023-0.06 mg/mL. Antimicrobial screening determined the inhibitory ability of almost all complexes for concentrations of 1 and 2 mg/mL, with zones of inhibition in the range of 9-19 mm. Examination of the interaction of BSA with Ag(I) complexes revealed the same change in the absorption maximum (hyperchromic shift) in the region of about 205 nm, which indicates that the interaction of BSA and the complex results in conformational changes of BSA.  

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