scholarly journals Short Overview of Some Assays for the Measurement of Antioxidant Activity of Natural Products and Their Relevance in Dermatology

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5301
Author(s):  
Morana Jaganjac ◽  
Vesna Sredoja Tisma ◽  
Neven Zarkovic
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Review Paper ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Redox Homeostasis ◽  
General Interest ◽  
Short Overview ◽  
Biomedical Interest ◽  
Selection Of

Impaired systemic redox homeostasis is implicated in the onset and development of various diseases, including skin diseases. Therefore, continuous search for natural products with antioxidant bioactivities applicable in biomedicine is attractive topic of general interest. Research efforts aiming to validate antioxidant potentials of natural products has led to the development of several assays based on various test principles. Hence, understanding the advantages and limitations of various assays is important for selection of assays useful to study antioxidant and related bioactivities of natural products of biomedical interest. This review paper gives a short overview on some chemical and cellular bioassays used to estimate the antioxidant activity of chosen natural products together with a brief overview on the use of natural products with antioxidant activities as adjuvant medicinal remedies in dermatology.

scholarly journals Comparison of the Antioxidant Activity of Malaysian Ginger (Zingiber officinale Roscoe) Extracts with that of Selected Natural Products and its Effect on the Viability of Myoblast Cells in Culture

2021 ◽  
Vol 50 (5) ◽  
pp. 1445-1456
Author(s):  
Nur Fatin Nabilah Mohd Sahardi ◽  
Faizul Jaafar ◽  
Siti Nor Asyikin Zakaria ◽  
Jen Kit Tan ◽  
Mariam Firdhaus Mad Nordin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Centella Asiatica ◽  
Butylated Hydroxytoluene ◽  
Therapeutic Effects ◽  
Antioxidant Power ◽  
Ficus Deltoidea

Ginger has been proven to possess various therapeutic effects, including antibacterial, anticancer, anti-inflammatory, and antioxidant effects. However, data on the comparison of ginger antioxidant activity with that of other natural products are still lacking. This study aimed to analyse and compare the antioxidant properties of two types of Malaysian ginger extracts (GE1 and GE2) with that of selected natural products. The antioxidant activities were measured by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and ferric reducing antioxidant power (FRAP) assays, while cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfonyl)-2H-tetrazolium (MTS) assay. The order of the DPPH scavenging activities was as follows: vitamin C > palm tocotrienol-rich fraction (TRF) > á-tocopherol > N-acetylcysteine (NAC) > Ficus deltoidea > butylated hydroxytoluene (BHT) > Centella asiatica > GE2 > GE1 > Moringa oleifera > Kelulut honey; the order of the mean FRAP value was as follows: NAC > á-tocopherol > BHT > TRF > Ficus deltoidea > Moringa oleifera > GE2 = GE1 > Centella asiatica > Kelulut honey. The viability assays showed that both ginger extracts significantly increased the percentage of viable cells (p < 0.05). In conclusion, neither of the ginger extracts was cytotoxic toward cells and both possessed comparable antioxidant properties, indicating their potential for ameliorating oxidative stress.

scholarly journals Phytochemistry and Antioxidant Activities of the Methanolic Leaf Extract of Clerodendrum splendens (Lamiaceae)

2018 ◽  
pp. 12-20
Author(s):  
Nganso Ditchou Yves Oscar ◽  
Amang A Ngoung GA ◽  
Soh Desire ◽  
Simo Nemg Fredy Brice ◽  
Nyasse Barthelemy
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Maximum Activity ◽  
Hepatic Lipids ◽  
Ic50 Value ◽  
Ic50 Values

This paper aimed at studying the antioxidant efficacy of the methanolic leaf extract of Clerodendrum splendens, a plant of the Lamiaceae family. Phytochemical tests carried out on extracts of Clerodendrum splendens leaves have been able to detect the presence of secondary metabolites such as Flavonoids, Tannins, Saponins and Terpenoids. The results of the antioxidant activity have shown that CSF2, CSF3 fractions and CSB, CSG fractions similarly inhibited hepatic lipids but significantly less than vitamin C. Compared to all fractions, the CSB fraction shows the best inhibitor on the peroxidation of hepatic lipids because at 150 μg/mL, there is a maximum activity (2.5 μg/mL of protein). However, it is found that CSF3, CSF2 and CSG have higher IC50 values than vitamin C (5.613±0.117) while CSEB, CSB and CSC fractions showed lower IC50 values than vitamin C, which is used as the reference reducing compound. The lower the IC50 value compared to vitamin C, the greater the antioxidant capacity of the plant extract. The results of this study suggest that Clerodendrum splendens represents an untapped source of compounds with potential antioxidant activity that could be explored in the development of new therapeutic natural products.

scholarly journals A DESCRIPTIVE STUDY AND IN-VITRO ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF TRIDAX PROCUMBENS LINN

2021 ◽  
Vol 11 (6) ◽  
pp. 1-4
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ferric Chloride ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Alcoholic Extract ◽  
Tridax Procumbens

Tridax Procumbens Linn is a member of the Asteraceae family. Tridax Procumbens has to be utilized because native medication intended for a number of complaints and problems in humans and animals for thousands of years. It is used widely in Indian conventional remedies for healing of wounds, like anticoagulants, in fungal infection, in diarrhea and dysentery, as an antioxidant, antimicrobial, anti-inflammatory, and immunomodulators. In folk medicine, certain communicable skin diseases are treated by using leaves extract. It's also known as 'Bhringraj,' an ayurvedic drug used to treat liver problems. At least 12,000 people have been separated from their families. These compounds protect plants from pathogens, insects, and herbivores by acting as defensive mechanisms. The aim of the test is to establish the antioxidant potential of the leaves of Tridax Procumbens. The current research is aimed at identifying novel plant directions, and antioxidant activity has been chosen for that reason. Using the maceration procedure, the power of the plant's shade dried leaves was extracted with chloroform water and ethanol. The antioxidant activities of the resulting extracts were evaluated using 2 techniques: nitric oxide scavenging activity and ferric chloride reductive ability. The alcoholic extract in 600 mg/ml and 800 mg/ml and 1000 mg/ml concentration has demonstrated antioxidant activity higher than ascorbic acid (20 mg) by nitric oxide scavenging method. By using a ferric chloride scavenging model, the aqueous and alcoholic extracts at 400 g/ml and 600 g/ml concentrations revealed antioxidant activity near to that of ascorbic acid (20 g).

scholarly journals A DESCRIPTIVE STUDY AND IN-VITRO ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF TRIDAX PROCUMBENS LINN

2021 ◽  
Vol IC1 (1) ◽  
pp. 1-4
Author(s):  
Hemant P. Suryawanshi
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ferric Chloride ◽  
Skin Diseases ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Alcoholic Extract ◽  
Tridax Procumbens

Tridax Procumbens Linn is a member of the Asteraceae family. Tridax Procumbens has to be utilized because native medication intended for a number of complaints and problems in humans and animals for thousands of years. It is used widely in Indian conventional remedies for healing of wounds, like anticoagulants, in fungal infection, in diarrhea and dysentery, as an antioxidant, antimicrobial, anti-inflammatory, and immunomodulators. In folk medicine, certain communicable skin diseases are treated by using leaves extract. It's also known as 'Bhringraj,' an ayurvedic drug used to treat liver problems. At least 12,000 people have been separated from their families. These compounds protect plants from pathogens, insects, and herbivores by acting as defensive mechanisms. The aim of the test is to establish the antioxidant potential of the leaves of Tridax Procumbens. The current research is aimed at identifying novel plant directions, and antioxidant activity has been chosen for that reason. Using the maceration procedure, the power of the plant's shade dried leaves was extracted with chloroform water and ethanol. The antioxidant activities of the resulting extracts were evaluated using 2 techniques: nitric oxide scavenging activity and ferric chloride reductive ability. The alcoholic extract in 600 mg/ml and 800 mg/ml and 1000 mg/ml concentration has demonstrated antioxidant activity higher than ascorbic acid (20 mg) by nitric oxide scavenging method. By using a ferric chloride scavenging model, the aqueous and alcoholic extracts at 400 g/ml and 600 g/ml concentrations revealed antioxidant activity near to that of ascorbic acid (20 g).

scholarly journals Fruit Pomological, Phytochemical Characteristic and Mineral Content of Rosehip Genotypes

2020 ◽  
pp. 18-23
Author(s):  
Nihal Acarsoy Bilgin ◽  
◽  
Adalet Mısırlı ◽  
Fatih Şen ◽  
Bilge Türk ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Vitamin C ◽  
Mineral Content ◽  
Antioxidant Activities ◽  
Total Phenol ◽  
High Demand ◽  
Nutrition And Health ◽  
Mineral Contents

Rosehips, which are commonly grown in nature in shrub form, are always popular due to their importance in terms of nutrition and health. Because fruits are rich in vitamin C, total phenol and mineral contents and antioxidant activities. Nowadays, there is high demand for natural products. Rosehip grows spontaneously in Turkey. In this study, 9 individuals were examined. Consequently, significant differences were stated according to genotypes for pomological and phytochemical characteristics. Vitamin C, phenol and antioxidant activity were found to affect P and Zn nutrients. Additionally, it was detected that P, K, Ca, Mg, Na Fe, Cu, Zn and Mn contents ranged in 0.09-0.17%, 1.35-1.86%, 1.05-1.44%, 0.43-069%, 50.00-100.00 ppm 28.50-48.30 ppm, 3.30-7.00 ppm, 8.06-12.56 ppm and 50.85-85.35 ppm, respectively. In this context, one of the genotypes has been found promising due to its content.

scholarly journals FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing

2016 ◽  
Vol 21 (3) ◽  
pp. 767-779 ◽  
Author(s):  
Renata Nunes Oliveira ◽  
Maurício Cordeiro Mancini ◽  
Fernando Cabral Salles de Oliveira ◽  
Thayse Marques Passos ◽  
Brid Quilty ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Natural Products ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
The Other ◽  
Ftir Analysis ◽  
Flavonoid Content ◽  
Dragon’S Blood ◽  
Brazilian Propolis

ABSTRACT Natural products are used in wound healing in order to prevent infection. Propolis is a well known antimicrobial with phenolic compounds and flavonoid content which vary according to the propolis origin. Besides propolis (from both Brazilian and UK sources), pomegranate, dragon's blood and sage are possible antimicrobials to be used in biomaterials. The goal of this work was to analyze the amount of phenols and flavonoid compounds in these natural products, their antioxidant activities and the bonds present by FTIR. The FTIR analysis revealed the presence of active compounds in all drug samples. The phenols quantification showed that Brazilian propolis was rich in phenols compared to the other drugs, followed by pomegranate and UK propolis. UK propolis was the most rich in flavonoids, which is expected on account of its origin. Pomegranate, UK propolis and Dragon's blood presented the highest antioxidant activity. All samples presented antioxidant activity > 82%.

scholarly journals Investigation of Antioxidant Activity and Physicochemical Properties of Local and Branded Honeys in Sindh, Pakistan

2020 ◽  
pp. 43-50
Author(s):  
Muhammad Farooque Lanjwani ◽  
Fayaz Ahmed channa
Keyword(s):  
Electrical Conductivity ◽  
Antioxidant Activity ◽  
Natural Products ◽  
Physicochemical Properties ◽  
Honey Bees ◽  
Antioxidant Activities ◽  
International Markets ◽  
Human Consumption ◽  
Average Value

The honey is a sweetest natural products because it have unique properties, which honey bees are collect from dissimilar plants materials. The honey is used as a nutritional purposes for human consumption and also use for medicinal and industrial purposes. The honey also an important product in the international markets. The present study was conducted to analyzed the physicochemical and antioxidant activities of different honey samples in Sindh Pakistan. The 15 honey samples were analyzed for parameters including pH, electrical conductivity, moisture, viscosity, ash%, density and antioxidant activity. The average value of pH was founded 3.78 electrical conductivity 2492 μs/cm, viscosity 12.34 mpa, moisture 15.99%, density 0.78 and average value of antioxidant activity was founded 19.12 mg/100g.

scholarly journals Antioxidant Activity of Natural Products Isolated from Red Seaweeds

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Caio Cesar Richter Nogueira ◽  
Izabel Christina Nunes de Palmer Paixão ◽  
Valéria Laneuville Teixeira
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Antioxidant Activities ◽  
Red Seaweeds

The present work describes more than 60 natural products from marine red seaweeds (Rhodophyta) and their antioxidant activities. The results indicate that algae belonging to the order Ceramiales, family Rhodomelaceae are the most promising as potential producers of antioxidants. This activity seems to be related to the ability to synthesize polyphenols and their derivatives, as bromophenols.

scholarly journals Isolation, selection of Lactobacillus strains with probiotic and antioxidant activities

2019 ◽  
Vol 14 (9) ◽  
pp. 170
Author(s):  
Tran Thi Ngoc Diep ◽  
Nguyen Thuy Huong
Keyword(s):  
Antioxidant Activity ◽  
Lactobacillus Plantarum ◽  
Functional Food ◽  
Antioxidant Activities ◽  
Lactobacillus Brevis ◽  
Fermented Food ◽  
Screening And Identification ◽  
High Antioxidant Activity ◽  
Probiotic Activity ◽  
Selection Of

This study was conducted to collect Lactobacillus strains which having probiotic and antioxidant activities from traditional Vietnamese fermented food. After isolation, screening and identification, we selected two strains, Lactobacillus plantarum and Lactobacillus brevis with probiotic activity and high antioxidant activity. These strains would be the microbiological agent for further studies about probiotic functional food with antioxidant and other valuable activities.

Determination of Antioxidant Activity In Vitro from Hyptis rhomboidea Mart. et Gal. Ethanol Extracts

2014 ◽  
Vol 472 ◽  
pp. 824-828 ◽  
Author(s):  
Xi Feng Li ◽  
Lu Tang ◽  
Nan Nan Zheng ◽  
Shuo An ◽  
You Xu Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Positive Control ◽  
Fresh Market ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Selection Of

Antioxidants are the chemical substances that reduce or prevent oxidation. The present study aimed to assess in vitro antioxidant activities of ethanol extracts Hyptis rhomboidea. DPPH, ABTS and FRAP assays were used for characterization of antioxidant activities of several extracts. Eventually, the promising extract was the ethyl acetate (EAc) extract, The analyzed extracts exhibited that antioxidant activities varied considerably as function of extracts. The highest DPPH• scavenging ability was found in Y6-8，which was divided from EAc by silica column, the lowest IC50 value (6.9μg/ml), followed by Y6-9 (11.7μg/ml) and the TEAC of Y6-8 and Y6-9were greater than positive control trolox. The same tendency was observed using ABTS and FRAP assays. These results suggest that the H. rhomboidea shows a better antioxidant activity, especially in the EAc extract and these results would be a guide in the selection of potential cultivars which are used in food and chemical industry and fresh market.

Sign in / Sign up

Export Citation Format

Share Document