scholarly journals The Antimicrobial Activities of Silver Nanoparticles from Aqueous Extract of Grape Seeds against Pathogenic Bacteria and Fungi

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 6081
Author(s):  
Fatimah Al-Otibi ◽  
Shahad K. Alkhudhair ◽  
Raedah I. Alharbi ◽  
Abdulaziz A. Al-Askar ◽  
Reem M. Aljowaie ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Uv Spectroscopy ◽  
Grape Seed ◽  
Antimicrobial Activities ◽  
Fungal Species ◽  
Gas Chromatography Mass Spectrometry ◽  
Grape Seeds ◽  
Antibacterial And Antifungal Activities

Grape seed extract (GSE) is a natural source of polyphenolic compounds and secondary metabolites, which have been tested for their possible antimicrobial activities. In the current study, we tested the antibacterial and antifungal activities of aqueous GSE and the biosynthesized silver nanoparticles loaded with GSE (GSE-AgNPs) against different pathogens. The biosynthesized GSE-AgNPs were assessed by UV spectroscopy, dynamic light scattering (DLS), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and gas chromatography/mass spectrometry (GC/MS). The antimicrobial activities were assessed against different bacterial and fungal species. DLS analysis showed that GSE-AgNPs had a Z-Average of 91.89 nm while UV spectroscopy showed that GSE-AgNPs had the highest absorbance at a wavelength of ~415 nm. FTIR analysis revealed that both of GSE and GSE-AgNPs consisted of different functional groups, such as hydroxyl, alkenes, alkyne, and aromatic rings. Both FE-SEM and TEM showed that GSE-AgNPs had larger sizes and rough surfaces than GSE and AgNO3. The results showed significant antimicrobial activities of GSE-AgNPs against all tested species, unlike GSE, which had weaker and limited effects. More studies are needed to investigate the other antimicrobial activities of GSE.

scholarly journals Photorhabdus sp. ETL Antimicrobial Properties and Characterization of Its Secondary Metabolites by Gas Chromatography–Mass Spectrometry

Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 787
Author(s):  
Tshikala Eddie Lulamba ◽  
Ezekiel Green ◽  
Mahloro Hope Serepa-Dlamini
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Insect Pests ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Toxigenic Fungi ◽  
Properties And Characterization

Entomopathogenic nematodes (EPNs) are known to be highly pathogenic to insect pests, due to their associated symbiotic bacteria, which produce virulence factors, exo-enzymes and other harmful secondary metabolites to conquer, kill, and degrade their insect hosts. However, these properties are not fully characterized. This study reports on the antimicrobial activities of Photorhabdus sp. strain ETL, symbiotically associated to an insect pathogenic nematode, Heterorhabditis zealandica, against human pathogenic bacteria and toxigenic fungi, as well as the non-targeted profiling of its secondary metabolites (SMs) using gas chromatography coupled to high-resolution time-of-flight mass spectrometry. Fatty acids including 3-eicosene, (E)-; 5-eicosene, (E)-; eicosene; 9-octadecenamide; undecanoic acid with shown antimicrobial activities were detected. This provided more insight on the composition and bioactivities of SMs produced by the Photorhabdus sp.

scholarly journals Potential Decontamination of Drinking Water Pathogens through k-Carrageenan Integrated Green Bottle Fly Bio-Synthesized Silver Nanoparticles

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1936 ◽  
Author(s):  
M. A. Abu-Saied ◽  
Mohamed Elnouby ◽  
Tarek Taha ◽  
Muhammad El-shafeey ◽  
Ali G. Alshehri ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Drinking Water ◽  
Pathogenic Bacteria ◽  
Polymeric Nanoparticles ◽  
Wide Distribution ◽  
Spectrophotometric Analysis ◽  
Transmission Electron ◽  
Green Bottle

The wide distribution of infections-related pathogenic microbes is almost related to the contamination of food and/or drinking water. The current applied treatments face some limitations. In the current study, k-carrageenan polymer was used as supporting material for the proper/unreleased silver nanoparticles that showed strong antimicrobial activity against six pathogenic bacteria and yeast. The bio-extract of the pupa of green bottle fly was used as the main agent for the synthesis of silver nanoparticles. The qualitative investigation of biologically synthesized silver nanoparticles was determined using UV-Vis spectrophotometric analysis; however, the size of nanoparticles was in range of 30–100 nm, as confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle size analyzer. The proper integration of silver nanoparticles into the polymeric substrate was also characterized through fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), SEM, and tensile strength. The antimicrobial activity of k-carrageenan/silver nanoparticles against Gram positive, Gram negative, and yeast pathogens was highly effective. These results indicate the probable exploitation of the polymeric/nanoparticles composite as an extra stage in water purification systems in homes or even at water treatment plants.

scholarly journals Synthesis of silver nanoparticles: double-green approach of using chitosan and microwave technique towards antimicrobial activity against pathogenic bacteria

2020 ◽  
Vol 10 (4) ◽  
pp. 5918-5922 ◽  
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Size Range ◽  
Low Cost ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Heterogeneous Distribution ◽  
E Coli ◽  
Green Approach ◽  
Dual Mechanism

A double green approach to the synthesis of silver nanoparticles using chitosan as a reducing agent and microwave radiation has been carried out in this study. Chitosan was extracted from marine crab carapace and the morphology of the produced chitosan was characterized using Scanning Electron Microscope (SEM). The production of silver nanoparticles (AgNPs) was monitored by the formation of surface plasmon resonance (SPR) at the λmax= 420 nm, indicates the reduction of the oxidation state of Ag+ to Ag0. The XRD data confirms the crystallinity nature of obtained AgNps, while the SEM and TEM revealed the formation of near-spherical shapes, polydisperse, heterogeneous distribution of AgNps with the size range between 7 to 25 nm. The antimicrobial activities of the AgNPs were investigated using The prepared AgNPs demonstrated dual mechanism action with chitosan, which acted as a stabilizer on the surface of AgNPs, and enhance the inhibition zone against E. tarda and E. coli. Therefore, the synthesized AgNPs in this study may have excellent potential for clinical application as it is green, low-cost and eco-friendly.

scholarly journals Coconut inflorescence sap mediated synthesis of silver nanoparticles and its diverse antimicrobial properties

2019 ◽  
Author(s):  
M.K. Rajesh ◽  
K.S. Muralikrishna ◽  
Swapna S. Nair ◽  
B. Krishna Kumar ◽  
T.M. Subrahmanya ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Secondary Metabolites ◽  
Hela Cells ◽  
Pathogenic Bacteria ◽  
Incubation Temperature ◽  
Antimicrobial Property ◽  
Antimicrobial Properties ◽  
Biological Origin ◽  
Synthesis Of Nanoparticles

ABSTRACTGreen synthesis of nanoparticles (NPs) involves the use of diverse extracts of biological origin as substrates to synthesize nanoparticles and can overcome the hazards associated with chemical methods. Coconut inflorescence sap, which is unfermented phloem sap obtained by tapping of coconut inflorescence, is a rich source of sugars and secondary metabolites. In this study, coconut inflorescence sap was used to synthesize silver nanoparticles (AgNPs). We have initially undertaken metabolomic profiling of coconut inflorescence sap from West Coast Tall cultivar to delineate its individual components. Secondary metabolites constituted the major portion of the inflorescence sap along with sugars, lipids and, peptides. The concentration of silver nitrate, inflorescence sap and incubation temperature for synthesis of AgNPs were optimized. Incubating the reaction mixture at 40°C was found to enhance AgNP synthesis. The AgNPs synthesized were characterized using UV-Visible spectrophotometry, X-Ray Diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FESEM) and Transmission Electron Microscopy (TEM). Antimicrobial property of AgNP was tested in tissue culture of arecanut (Areca catechu L.) where bacterial contamination (Bacillus pumilus) was a frequent occurrence. Significant reduction in the contamination was observed when plantlets were treated with aqueous solutions of 0.01, 0.02 and 0.03% of AgNPs for one hour. Notably, treatment with AgNPs did not affect growth and development of the arecanut plantlets. Cytotoxicity of AgNPs was quantified in HeLa cells. Viability (%) of HeLa cells declined significantly at 10 ppm concentration of AgNP and complete mortality was observed at 60 ppm. Antimicrobial properties of AgNPs synthesized from inflorescence sap were also evaluated and confirmed in human pathogenic bacteria viz., Salmonella sp., Vibrio parahaemolyticus, and Escherichia coli. The study concludes that unfermented inflorescence sap, with above neutral pH, serves as an excellent reducing agent to synthesize AgNPs from Ag+. Graphical abstract

Chemical composition, antioxidant, and antimicrobial activities of two essential oils from Algerian propolis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Safia Boulechfar ◽  
Amar Zellagui ◽  
Meltem Asan-Ozusaglam ◽  
Chawki Bensouici ◽  
Ramazan Erenler ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Reducing Ability ◽  
Antioxidant And Antimicrobial Activity ◽  
Fungistatic Effect

Abstract This study aims to investigate the chemical composition, antioxidant, and antimicrobial activity of two essential oils (EOs) from Algerian propolis. The volatile constituents were analyzed by gas chromatography-mass spectrometry. Fifty components were identified from the oils. The major components were found to be: cedrol (17.0%), β-eudesmol (7.7%), and α-eudesmol (6.7%) in EO of propolis from Oum El Bouaghi (EOPO) whilst α-pinene (56.1%), cis-verbenol (6.0%), and cyclohexene,3-acetoxy-4-(1-hydroxy-1-methylethyl)-1-methyl (4.4%) in EO of propolis from Batna (EOPB). The antioxidant properties of EOPO and EOPB were determined using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) and cupric reducing antioxidant capacity (CUPRAC assays), respectively. Both EOs had more cupric ion reducing ability than scavenging ABTS•+ radicals. The antimicrobial potential of the two EOs against eight pathogens was assayed by the agar diffusion method and the mode of action was determined by microdilution assay. The results revealed that EOPB was bactericidal for all tested pathogenic bacteria and fungicidal for Candida albicans ATCC 10231, whereas, EOPO showed bacteriostatic effect against Escherichia coli O157:H7 and Pseudomonas aeruginosa ATCC27853 and fungistatic effect against C. albicans ATCC 10231. Thus, the obtained results suggest the important use of propolis EOs as preservative agents.

scholarly journals Antimicrobial Activity of Essential Oil from Seeds of Carum carvi and Its Composition

1970 ◽  
Vol 25 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Jaripa Begum ◽  
M Nazrul Islam Bhuiyan ◽  
Jasmin Uddin Chowdhury ◽  
M Nuzmul Hoque ◽  
M Nural Anwar
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Phytopathogenic Fungi ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimum Fungicidal Concentration ◽  
Carum Carvi ◽  
Main Components

The essential oil of Carum carvi L. seeds was screened for its antimicrobial activity against ten pathogenic bacteria and six phytopathogenic fungi. The essential oil showed promising inhibitory activity against all the test bacteria, even at 2 ìl/disc. The minimum inhibitory concentration (MIC, 100-300 ppm) and minimum bactericidal concentration (MBC, 200-400 ppm) values of essential oil were determined. The antifungal screening of the essential oil showed 100% inhibition of radial mycelial growth of all the test fungi at 100 ppm. The MIC and minimum fungicidal concentration (MFC) values were found to vary from 50-300 ppm and 200-400 ppm respectively. The essential oil extracted by hydrodistillation from seeds of C. carvi was analyzed by gas chromatography-mass spectrometry (GC-MS). About 10 compounds had been identified in the seeds oils, accounting for more than 99.7% of the oils. The main components of the seeds oil were thymol (48.20%), o-cymene (19.29%), ϒ-terpinen (17.61%) and trimethylene dichloride (8.81%). Keywords: Antimicrobial activities; Carum carvi; Essential oil; GC-MS; ThymolDOI: http://dx.doi.org/10.3329/bjm.v25i2.4867 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 85-89

scholarly journals Bioactive Nano-Composite Multifilament Yarns

2012 ◽  
Vol 7 (1) ◽  
pp. 155892501200700 ◽  
Author(s):  
Mohammad Esmail Yazdanshenas ◽  
Rogheih Damerchely ◽  
Abo Saied Rashidi ◽  
Ramin Khajavi
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Inductively Coupled Plasma ◽  
Melt Spinning ◽  
Antimicrobial Properties ◽  
Physical And Mechanical Properties ◽  
Nylon 6 ◽  
Antimicrobial Activities ◽  
Scanning Calorimetry ◽  
Inductively Coupled

Physical, mechanical and antimicrobial properties of nylon 6 (polycaprolactam) doped with different amounts of silver nanoparticles were investigated in this study. Two series of filament yarns counts (20 and 70 Denier) were produced by melt spinning method with different amounts of silver nanoparticles contents (0, 0.5, 1 and 4 wt%). Elemental analysis of silver and titanium dioxide (present in polymer as delustering agent) was carried out by energy dispersive X-ray (EDX) and inductively coupled plasma mass spectrometry (ICP-MS) methods. Tensile testing, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and differential scanning calorimetry (DSC) were used to characterize the yarns. Antimicrobial activities were quantitatively evaluated against Escherichia Coli (gram-negative) and Staphylococcus Aureus (gram-positive) bacteria. The doped nylon 6 fibers showed a well dispersed distribution of silver nanoparticles. Yarns with 0.5–1.0 wt% of silver nanoparticle content were found to have improved physical and mechanical properties, as well as, significant antimicrobial activity.

scholarly journals Bridelia retusa (L.) Spreng. Fruits: Antimicrobial Efficiency and their Phytochemical Constituents

2016 ◽  
Vol 8 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Aniel Kumar OWK ◽  
Mutyala Naidu LAGUDU
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Fungal Species ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Efficiency ◽  
Bridelia Retusa

Antimicrobial analysis of hexane, chloroform, methanol and aqueous extracts of Bridelia retusa fruits was performed by agar well method and minimum inhibitory concentration was determined by serial two-fold dilution method. Seven human pathogenic bacteria species including Gram positive and Gram negative bacteria and three fungal species were used in the study and the results indicated that the Gram positive bacteria and fungi were more sensitive than the Gram negative bacteria, to both solvent and aqueous Bridelia retusa fruit extracts. Moreover, Enterococcus faecalis was found as the most sensitive bacteria, whereas Proteus vulgaris and Aspergillu niger were the most resistant to the tested extracts. Phytochemical analysis of fruits revealed the presence of secondary metabolites like alkaloid, saponins and terpenoids, which have been implicated in antimicrobial activities. Hence, it would be recommended to explore the maximum potential of Bridelia retusa in the medicinal and pharmaceutical field and investigation are endorsed for further application useful in phytomedicine.

scholarly journals Chemical Composition and Antimicrobial Activities of the Essential Oil From Salvia mirzayanii Leaves

2017 ◽  
Vol 22 (4) ◽  
pp. 770-776 ◽  
Author(s):  
Kamiar Zomorodian ◽  
Mahmoodreza Moein ◽  
Keyvan Pakshir ◽  
Forough Karami ◽  
Zahra Sabahi
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Antifungal Activities ◽  
Global Challenge ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal

Resistance of many pathogens to available drugs is a global challenge and is leading to growing interest in natural alternative products. In this study, chemical composition and in vitro antibacterial and antifungal activities of the essential oil from Salvia mirzayanii were investigated. The chemical constituents of essential oil from S mirzayanii were analyzed by gas chromatography–mass spectrometry. The antimicrobial activity was determined by broth microdilution. The main identified compounds were 1,8-cineole (41.2 ± 1.3%), linalool acetate (11.0 ± 0.5%), and α-terpinyl acetate (6.0 ± 0.4%) (mL of essential oil/g of plant material). The MIC95 were 0.03 to 0.5 µL/mL and 16 to 128 µL/mL for gram-positive and gram-negative bacteria, respectively. These results indicated that Salvia mirzayanii essential oil significantly inhibited the growth of standard and clinically isolated tested yeasts by MIC50 0.03 to 1 µL/mL. Potent antibacterial and antifungal activities of Salvia mirzayanii essential oil may be considered in future study, particularly against antibiotic-resistant cases.

scholarly journals Synthesis, Characterization and Evaluation of Biological Activity of some Substituted(E)-2-Benzylidenehydrazinecarbothio Amides

2015 ◽  
Vol 59 ◽  
pp. 199-211
Author(s):  
R. Vijayakumar ◽  
M. Rajarajan ◽  
R. Senbagam ◽  
S. Balaji ◽  
V. Manikandan ◽  
...  
Keyword(s):  
Biological Activity ◽  
Nmr Spectra ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Fungal Species ◽  
Significant Activity ◽  
Ir And Nmr Spectra ◽  
Physical Constants ◽  
Human Pathogenic Bacteria ◽  
Substituted Benzaldehydes

A series of substituted (E)-2-benzylidenehydrazinecarbothioamide compounds were synthesized by condensation of thiosemicarbazide with substituted benzaldehydes. The synthesized substituted (E)-2-benzylidenehydrazinecarbothioamide compounds were characterized by their physical constants, UV, IR and NMR spectra. The antimicrobial activities of these synthesized substituted (E)-2-benzylidenehydrazinecarbothioamide compounds have been screened by Bauer-Kirby method using human pathogenic bacteria and fungal species. The antimicrobial activities of all synthesized (E)-2-benzylidenehydrazinecarbothioamide compounds have shown significant activity.

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