Chemical and Biological Characteristics of Oxytropis pseudoglandulosa Plant of Mongolian Origin

Oxytropis pseudoglandulosa is used in Mongolian traditional medicine due to its numerous reported health-promoting effects. To date, there are very few scientific reports that describe this species. In this article, its volatile oil composition, lipid extract composition, total phenolic and flavonoid content, antibacterial and allergenic properties are elucidated for the first time. Hexadecanoic acid, fokienol and tricosane were determined as the most notable components of the volatile oil, at 13.13, 11.46 and 5.55%, respectively. Methyl benzoate was shown to be the most abundant component of lipid extract at 40.69, followed by (E)-prop-2-enoic acid, 3-phenyl- and benzenepropanoic acid, at 18.55 and 9.97%. With a TPC of 6.620 mg GAE g−1 and TFC of 10.316 mg QE g−1, the plant extract of O. pseudoglandulosa indicated good antioxidant activity measured by IC50 at 18.761 µg mL−1. Of the 12 tested microorganisms, B. subtilis and S. cerevisiae were the shown to be most susceptible to the plant extract, with MIC at 2.081 and 0.260% (v/v), respectively. Bet v 1—a major birch pollen allergen found in plant-based foods—was determined to be at 192.02 ng g−1 with ELISA. Such a wide spectrum of biological activity indicated by O. pseudoglandulosa lends credence for its application in food industry. Its exerted antioxidant and antimicrobial effects could improve preservation of low-processed food dedicated for consumers afflicted with allergies. Hexadecanoic acid supplemented in foods with dietary plant extracts could add to the potential anti-inflammatory impact. The analysis of lipid makeup suggests O. pseudoglandulosa extract could also be considered as natural pesticide in organic farming.

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Chemical and Biological Profile and Allergenicity of Thymus baicalensis Plant of Mongolian Origin

Antioxidants ◽

10.3390/antiox10121905 ◽

2021 ◽

Vol 10 (12) ◽

pp. 1905

Author(s):

Tuya Narangerel ◽

Michał Sójka ◽

Radosław Bonikowski ◽

Konrad Jastrząbek ◽

Witold Sroczyński ◽

...

Keyword(s):

Unsaturated Fatty Acids ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antimicrobial Properties ◽

Volatile Oil ◽

Inhibitory Effect ◽

Food Supplement ◽

Oil Composition ◽

Polyphenolic Composition ◽

Health Promoting

Thymus baicalensis is a medicinal plant recognized as a traditional Mongolian therapeutic and health-promoting food supplement. The aim of the study was to check the suitability of the tested plant for supporting the treatment of certain diseases. The following study is the first one to showcase the versatile scope of characteristics of T. baicalensis, including its volatile oil composition, polyphenolic composition, lipid composition, phenolic and flavonoid contents, antioxidant activity, antimicrobial properties and ingestive allergenicity. Myrcene, at 26.15%, was shown to be the most abundant component of the volatile oil. Compounds known as inherent components of the Thymus genus: thymol and carvacrol made up only about 0.24% of the extracted oil. As much as 10.11 g kg−1 of polyphenol compounds were identified as derivatives of luteolin-7-O-glucuronide. The lipid extract was found to be rich in palmitic acid (31.05%), while unsaturated fatty acids were not reported. Spectrophotometric determination of the phenols and flavonoids indicated 7.541 mg of gallic acid g−1 and 4.345 mg of quercitin g−1, respectively. The free radical scavenging activity was determined by the 2,2-difenylo-1-pikrylohydrazyl method at IC50 = 206.97 µg mL−1. The extracts also had a strong inhibitory effect on M. flavus and P. fluorescenes bacteria, as well as S. cerevisiae yeasts. The Bet v 1 and profilin allergens in T. baicalensis were reported at 175.17 ng g−1 and 1.66 ng g−1, respectively.

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Essential Oil Profile, Phenolic Content and Antioxidant Activity of Geranium kikianum

Natural Product Communications ◽

10.1177/1934578x1701200234 ◽

2017 ◽

Vol 12 (2) ◽

pp. 1934578X1701200 ◽

Cited By ~ 4

Author(s):

Sanja Ćavar Zeljković ◽

Kit Tan ◽

Sonja Siljak-Yakovlev ◽

Milka Maksimović

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Volatile Oil ◽

Total Phenolic ◽

Residual Water ◽

Oil Composition ◽

Phytochemical Investigation ◽

Dpph Method

This work presents the first phytochemical investigation of Geranium kikianum Kit Tan & G. Vold (Geraniaceae), a species endemic to the southern Peloponnese, Greece. The essential oil from aerial parts of the plant was isolated by hydrodistillation and its chemical composition characterized by GC-MS. A total of 26 compounds were identified, constituting 98.3% of the oil composition. Oxygenated sesquiterpenes were the main group of constituents (75.4%). The major component of the oil was the sesquiterpene ketone germacrone (45.6%). Eleven alcohols represented the most diverse chemical class in the volatile oil (36.3%), and phytol was the most abundant acyclic diterpene alcohol (11.4%). The volatile compounds and residual water remaining after hydrodistillation were screened for their radical-scavenging activity by the DPPH method. IC 50 values ranged from 0.2±0.03 mg/mL for the undiluted decoction to 69.7±0.5 mg/mL for the essential oil. The significant antioxidant activity of the residual water, comparable with that of the widespread synthetic antioxidant BHT (0.2±0.01 mg/mL), and almost tenfold higher than thymol (1.90 ± 0.04 mg/mL), correlates with a high content of total phenolic compounds (100.2±1.7 mg GAE/g), and flavonoids (11.9±1.2 mg GAE/g).

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Comparative Antioxidant Activity and Volatile Oil Composition of Leaves and Fruits of Thuja orientalis Growing in Egypt

Walailak Journal of Science and Technology (WJST) ◽

10.48048/wjst.2019.3269 ◽

2018 ◽

Vol 16 (11) ◽

pp. 823-830

Author(s):

Abeer MOAWAD ◽

Elham AMIN

Keyword(s):

Phenolic Content ◽

Northeast Asia ◽

Volatile Oil ◽

Geranyl Acetate ◽

Total Phenolic ◽

Alcoholic Extract ◽

Oil Composition ◽

Anti Oxidant ◽

Thuja Orientalis ◽

Fruit Oil

Thuja orientalis L. (Cupressaceae) is an evergreen arbor that is distributed throughout Northeast Asia as a common ornamental plant. The volatile oil of leaves and fruits of T. orientalis growing in Egypt was prepared by hydro-distillation followed by GC-MS analysis in order to compare between their compositions. Results revealed that fruits are richer in monoterpenes (62.5 %) while leaves are richer in sesquiterpenes (57 %). The major components in leaf oil are; α-cedrol (15.8 %), β-caryophyllene (15 %), α-humulene (10.7 %), d-limonene (7.3 %), α-pinene (6.9 %), β-myrecene (5.9 %) and α-terpinolene (5.2 %). On the other hand, fruit oil contains α-pinene (11.3 %), α-cedrol (11.2 %), β-myrecene (9.6 %), geranyl acetate (9 %) and β-caryophyllene (8.9 %) in major amounts. Comparison between the oil content in the Egyptian conifer and other reported conifers worldwide is also discussed. Comparative determination of total phenolic content (TPC), total flavonoids content (TFC) and anti-oxidant activity of alcoholic extract of both leaves and fruits showed that fruits content of flavonoids (0.6267±0.026 mg RE /g) is double the content of the leaves (0.3069±0.019 mg RE /g). Similarly, fruits contain higher phenolic content (0.75±0.007 mg GE/g) compared to 0.51±0.007 mg GE/g in leaves. Also, fruits extract exhibited higher anti-oxidant activity (2.3516±0.0 mg AEAC/g) than leaves (1.2734±0.0022 mg AEAC/g). The extracts of T. orientalis could be a valuable material for pharmaceutical industry.

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Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽

10.21273/hortsci.33.3.492f ◽

1998 ◽

Vol 33 (3) ◽

pp. 492f-493

Author(s):

Roberto F. Vieira ◽

James E. Simon ◽

Peter Goldsbrough ◽

Antonio Figueira

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Rapd Markers ◽

Volatile Oil ◽

Essential Oil Composition ◽

Oil Composition ◽

Traditional Medicines ◽

The Tropics ◽

High Degree ◽

First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

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New menthone type of Mentha pulegium L. volatile oil from northwest Iran

Czech Journal of Food Sciences ◽

10.17221/165/2009-cjfs ◽

2011 ◽

Vol 29 (No. 3) ◽

pp. 285-290 ◽

Cited By ~ 10

Author(s):

M.B. Hassanpouraghdam ◽

A.B. Akhgari ◽

M.A. Aazami ◽

J. Emarat-Pardaz

Keyword(s):

Essential Oil ◽

Volatile Oil ◽

Oil Composition ◽

Mentha Pulegium ◽

Food Industries ◽

Aerial Parts ◽

Sesquiterpene Hydrocarbons ◽

Main Class ◽

Northwest Iran ◽

A Minor

The constituents of the volatile oil of air-dried aerial parts of Mentha pulegium L. (Lamiaceae) plants wildly growing in Northwest Iran were analysed by GC/MS. 46 components were identified, comprising 96.6% of the essential oil. Monoterpenes (78.9%) were the main class of the identified components followed by a minor proportion of sesqui-<br />terpenes (11%). Oxygenated monoterpenes (75.3%) were the major subclass of volatile oil components with menthone (38.7%), menthol (11.3%), neomenthol (10.5%), and pulegone (6.8%) as major compounds. Sesquiterpene hydrocarbons (10.6%) were the highlighted subclass of 15 carbons sesquiterpenoidal compounds with (E)-caryophyllene (4.9%) and β-cubebene (2.5%) as their principle representatives. Furthermore, menthyl acetate (C<sub>12</sub> acetylated monoterpene derived compound) was contained considerable amounts (5.2%) in the essential oil. In total, volatile oil composition of M. pulegium L. plants studied in the present experiment was characterised as a new menthone type with appreciable amounts of menthol and neomenthol, and it could be used as a potential source of these high value monoterpenes in pharmaceutical and food industries.

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Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents

Foods ◽

10.3390/foods9101371 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1371 ◽

Cited By ~ 1

Author(s):

Vanesa Nuñez-Gómez ◽

Nieves Baenas ◽

Inma Navarro-González ◽

Javier García-Alonso ◽

Diego A. Moreno ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

Methyl Jasmonate ◽

Bioactive Compounds ◽

High Performance ◽

Total Phenolic ◽

Diode Array ◽

Total Carotenoids ◽

Health Promoting

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

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Purification of Birch Pollen Allergen Extract by Gel Filtration

International Archives of Allergy and Immunology ◽

10.1159/000232452 ◽

1980 ◽

Vol 61 (3) ◽

pp. 299-307 ◽

Cited By ~ 11

Author(s):

E. Puttonen ◽

L. Pilström

Keyword(s):

Gel Filtration ◽

Birch Pollen ◽

Pollen Allergen ◽

Allergen Extract ◽

Birch Pollen Allergen

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The Influence of Air Pollution on the Development of Allergic Inflammation in the Airways in Krakow’s Atopic and Non-Atopic Residents

Journal of Clinical Medicine ◽

10.3390/jcm10112383 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2383

Author(s):

Ewa Czarnobilska ◽

Małgorzata Bulanda ◽

Daniel Bulanda ◽

Marcel Mazur

Keyword(s):

Air Pollution ◽

Pearson Correlation ◽

Birch Pollen ◽

Allergic Inflammation ◽

Control Group ◽

Airway Diseases ◽

Birch Pollen Allergen ◽

Blood Basophil ◽

Simultaneous Activation ◽

The Difference

Until now, the simultaneous influence of air pollution assessed by measuring the objective marker of exposition (1-hydroxypirene, 1-OHP) and atopy on the development of allergic airway diseases has not been studied. The aim of this study was to determine the pathomechanism of the allergic response to PM2.5 in atopic and non-atopic patients. We investigated the changes in peripheral blood basophil activity of patients after stimulation with the birch pollen allergen alone, the allergen combined with PM2.5 (BP), PM2.5 alone, a concentration of 1-OHP in urine, and a distance of residence from the main road in 30 persons. Activation by dust alone was positive for all concentrations in 83% of atopic and 75% of non-atopic assays. In the group of people with atopy, the simultaneous activation of BP gave a higher percentage of active basophils compared to the sum of activation with dust and birch pollen alone (B + P) for all concentrations. The difference between BP and B + P was 117.5 (p = 0.02) at a PM concentration of 100 μg. Such a relationship was not observed in the control group. The correlation coefficient between the distance of residence from major roads and urinary 1-OHP was 0.62. A Pearson correlation analysis of quantitative variables was performed, and positive correlation results were obtained in the atopy group between BP and 1-OH-P. Exposure to birch pollen and PM2.5 has a synergistic effect in sensitized individuals. The higher the exposure to pollutants, the higher the synergistic basophil response to the allergen and PM in atopic patients.

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