scholarly journals Environmental Hazards of Boron and Vanadium Nanoparticles in the Terrestrial Ecosystem—A Case Study with Enchytraeus crypticus

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1937
Author(s):  
Angela Barreto ◽  
Joana Santos ◽  
Mónica J. B. Amorim ◽  
Vera L. Maria
Keyword(s):  
Avoidance Behavior ◽  
Anthropogenic Activities ◽  
Terrestrial Ecosystem ◽  
Population Level ◽  
Long Term Effects ◽  
Opposite Pattern ◽  
Toxicological Effects ◽  
Short Term Exposure ◽  
Enchytraeus Crypticus

From the start of the 21st century, nanoecotoxicological research has been growing in fast steps due to the need to evaluate the safety of the increasing use of engineered nanomaterials. Boron (B) and vanadium (V) nanoparticles (NPs) generated by anthropogenic activities are subsequently released in the environment; therefore, organisms can be continuously exposed to these NPs for short or long periods. However, the short and long-term effects of BNPs and VNPs on soil organisms are unknown. This work aimed to recognize and describe their potential toxicological effects on the model species Enchytraeus crypticus, assessing survival and reproduction, through a longer-term exposure (56 days (d)—OECD test extension of 28 d), and avoidance behavior, through a short-term exposure (48 hours (h)). After 28 d, BNPs did not induce a significant effect on E. crypticus survival, whereas they decreased the organisms’ reproduction at 500 mg/kg. From 10 to 500 mg/kg, VNPs decreased the E. crypticus survival and/or reproduction. After 56 d, 100 to 500 mg/kg BNPs and 50 to 500 mg/kg VNPs, decreased the reproduction output of E. crypticus. The estimated Effect Concentrations (ECx) based on reproduction, for BNPs, were lower at 56 d compared with 28 d; for VNPs, an opposite pattern was found: ECx 28 d < ECx 56 d. BNPs did not induce an avoidance behavior, but organisms avoided the soil contaminated with 10 mg VNPs/kg. The tested NPs showed different E. crypticus apical effects at 28 d from the ones detected at 56 d, dependent on the type of NPs (B vs. V). In general, VNPs showed to be more toxic than BNPs. However, the effects of VNPs were alleviated during the time of exposure, contrarily to BNPs (which became more toxic with extended duration). The present study adds important information about NPs toxicity with ecological significance (at the population level). Including long-term effects, the obtained results contributes to the improvement of NPs risk assessment.

scholarly journals Responses of Vertebrate Wildlife to Oil and Natural Gas Development: Patterns and Frontiers

2021 ◽  
Author(s):  
A. D. Chalfoun
Keyword(s):  
Natural Gas ◽  
Anthropogenic Activities ◽  
Spatial Scales ◽  
Habitat Type ◽  
Population Level ◽  
Low Frequencies ◽  
Natural Gas Development ◽  
The Usa ◽  
Oil And Natural Gas

Abstract Purpose of Review Anthropogenic activities can lead to the loss, fragmentation, and alteration of wildlife habitats. I reviewed the recent literature (2014–2019) focused on the responses of avian, mammalian, and herpetofaunal species to oil and natural gas development, a widespread and still-expanding land use worldwide. My primary goals were to identify any generalities in species’ responses to development and summarize remaining gaps in knowledge. To do so, I evaluated the directionality of a wide variety of responses in relation to taxon, location, development type, development metric, habitat type, and spatiotemporal aspects. Recent Findings Studies (n = 70) were restricted to the USA and Canada, and taxonomically biased towards birds and mammals. Longer studies, but not those incorporating multiple spatial scales, were more likely to detect significant responses. Negative responses of all types were present in relatively low frequencies across all taxa, locations, development types, and development metrics but were context-dependent. The directionality of responses by the same species often varied across studies or development metrics. Summary The state of knowledge about wildlife responses to oil and natural gas development has developed considerably, though many biases and gaps remain. Studies outside of North America and that focus on herpetofauna are lacking. Tests of mechanistic hypotheses for effects, long-term studies, assessment of response thresholds, and experimental designs that isolate the effects of different stimuli associated with development, remain critical. Moreover, tests of the efficacy of habitat mitigation efforts have been rare. Finally, investigations of the demographic effects of development across the full annual cycle were absent for non-game species and are critical for the estimation of population-level effects.

Do researchers impact their study populations? Assessing the effect of field procedures in a long term population monitoring of sea kraits

2012 ◽  
Vol 33 (3-4) ◽  
pp. 365-372 ◽  
Author(s):  
Thomas Fauvel ◽  
François Brischoux ◽  
Marine Jeanne Briand ◽  
Xavier Bonnet
Keyword(s):  
Body Condition ◽  
New Caledonia ◽  
Stress Hormones ◽  
Population Level ◽  
Population Monitoring ◽  
Long Term Effects ◽  
Negative Effects ◽  
Short Term ◽  
Head Morphology

Long term population monitoring is essential to ecological studies; however, field procedures may disturb individuals. Assessing this topic is important in worldwide declining taxa such as reptiles. Previous studies focussed on animal welfare issues and examined short-term effects (e.g. increase of stress hormones due to handling). Long-term effects with possible consequences at the population level remain poorly investigated. In the present study, we evaluated the effects of widely used field procedures (e.g. handling, marking, forced regurgitation) both on short-term (hormonal stress response) and on long-term (changes in body condition, survival) scales in two intensively monitored populations of sea kraits (Laticauda spp.) in New Caledonia. Focusing on the most intensively monitored sites, from 2002 to 2012, we gathered approximately 11 200 captures/recaptures on 4500 individuals. Each snake was individually marked (scale clipping + branding) and subjected to various measurements (e.g. body size, head morphology, palpation). In addition, a subsample of more than 500 snakes was forced to regurgitate their prey for dietary analyses. Handling caused a significant stress hormonal response, however we found no detrimental long-term effect on body condition. Forced regurgitation did not cause any significant effect on both body condition one year later and survival. These results suggest that the strong short-term stress provoked by field procedures did not translate into negative effects on the population. Although similar analyses are required to test the validity of our conclusions in other species, our results suggest distinguishing welfare and population issues to evaluate the potential impact of population surveys.

Long-term regulation of hexose uptake by isoproterenol in cultured 3T3 adipocytes

1985 ◽  
Vol 248 (6) ◽  
pp. E706-E711 ◽  
Author(s):  
J. P. van Putten ◽  
H. M. Krans
Keyword(s):  
Protein Synthesis ◽  
Prolonged Exposure ◽  
Term Effect ◽  
Long Term Effects ◽  
Fat Cell ◽  
Short Term ◽  
Long Term Effect ◽  
Short Term Exposure ◽  
Hexose Uptake

Catecholamines are known to have short-term regulatory effects on fat cell hexose uptake. We examined the long-term effects of catecholamines on the insulin-sensitive 2-deoxyglucose (dGlc) uptake in cultured 3T3-L1 adipocytes. Prolonged exposure (48 h) to isoproterenol (beta-adrenergic agonist) stimulated the basal dGlc uptake up to 90%. The effect was specific, time, concentration, and protein synthesis dependent and reversible. The effect of insulin was unaltered and superimposed on the increase in basal dGlc uptake. The long-term effect of isoproterenol was mimicked by epinephrine, dibutyryl cAMP (DBcAMP), and 1-methyl-3-isobutylxanthine (IBMX). By contrast, short-term exposure to isoproterenol (and epinephrine) induced a protein synthesis-independent increase in basal dGlc uptake (30%) not accompanied by an increase in insulin responsiveness. Moreover, on short-term basis, DBcAMP and IBMX suppressed both the basal and insulin-stimulated uptake up to 50%. Determination of the intracellular nonphosphorylated dGlc during the uptake and of the hexokinase activity revealed that the long-term effect of isoproterenol was most likely due to alterations low in dGlc transport. In conclusion, long-term regulators of hexose uptake are in cultured 3T3-L1 adipocytes, isoproterenol, and other cAMP stimulators. The long-term effect is independent from the short-term regulatory effect of the agents and from the effect of insulin.

Toxic effects of acid rain on aquatic and terrestrial ecosystems

1984 ◽  
Vol 62 (8) ◽  
pp. 986-990 ◽  
Author(s):  
G. K. Rutherford
Keyword(s):  
Terrestrial Ecosystems ◽  
Acid Precipitation ◽  
Forest Growth ◽  
Toxic Effects ◽  
Economic Damage ◽  
Soil Productivity ◽  
Biological Processes ◽  
Long Term Effects ◽  
Toxicological Effects

The historical perspective as well as the nature and causes of acid precipitation are presented. The toxicological effects of acid precipitation on lakes, other water bodies, fish, and invertebrate fauna are reviewed. In addition, the effects of this phenomenon on soil productivity and forest growth are examined. It appears that grave toxic effects have been and are being experienced by aquatic systems, but there is little reliable evidence of economic damage to crops, natural vegetation, and soil and biological processes. There may be insidious long-term effects on terrestrial ecosystems, particularly in the more susceptible areas.

Terrestrial ecosystem loss and biosphere collapse

2014 ◽  
Vol 25 (5) ◽  
pp. 542-563 ◽  
Author(s):  
Glen Barry
Keyword(s):  
Percolation Theory ◽  
Landscape Connectivity ◽  
Terrestrial Ecosystem ◽  
Terrestrial Ecosystems ◽  
Well Being ◽  
Spatially Explicit ◽  
Long Term Effects ◽  
Content Type ◽  
Intact Core

Purpose – The purpose of this paper is to propose a measurable terrestrial ecosystem boundary to answer the question: what extent of landscapes, bioregions, continents, and the global Earth System must remain as connected and intact core ecological areas and agro-ecological buffers to sustain local and regional ecosystem services as well as the biosphere commons? Design/methodology/approach – This observational study reviews planetary boundary, biosphere, climate, ecosystems, and ecological tipping point science. It presents a refinement to planetary boundary science to include a measurable terrestrial ecosystem boundary based on landscape ecology and percolation theory. The paper concludes with discussion of the urgency posed by ecosystem collapse. Findings – A new planetary boundary threshold is proposed based on ecology's percolation theory: that across scales 60 percent of terrestrial ecosystems must remain, setting the boundary at 66 percent as a precaution, to maintain key biogeochemical processes that sustain the biosphere and for ecosystems to remain the context for human endeavors. Strict protection is proposed for 44 percent of global land, 22 percent as agro-ecological buffers, and 33 percent as zones of sustainable human use. Research limitations/implications – It is not possible to carry out controlled experiments on Earth's one biosphere, removing landscape connectivity to see long-term effects results upon ecological well-being. Practical implications – Spatially explicit goals for the amount and connectivity of natural and agro-ecological ecosystems to maintain ecological connectivity across scales may help in planning land use, including protection and placement of ecological restoration activities. Originality/value – This paper proposes the first measureable and spatially explicit terrestrial ecosystem loss threshold as part of planetary boundary science.

scholarly journals Behavioral Response Study on Seismic Airgun and Vessel Exposures in Narwhals

2021 ◽  
Vol 8 ◽  
Author(s):  
Mads Peter Heide-Jørgensen ◽  
Susanna B. Blackwell ◽  
Outi M. Tervo ◽  
Adeline L. Samson ◽  
Eva Garde ◽  
...  
Keyword(s):  
Noise Exposure ◽  
Anthropogenic Activities ◽  
Travel Speed ◽  
The Arctic ◽  
Line Of Sight ◽  
Long Term Effects ◽  
Sound Exposure ◽  
Ice Coverage ◽  
Exposure Levels

One of the last pristine marine soundscapes, the Arctic, is exposed to increasing anthropogenic activities due to climate-induced decrease in sea ice coverage. In this study, we combined movement and behavioral data from animal-borne tags in a controlled sound exposure study to describe the reactions of narwhals, Monodon monoceros, to airgun pulses and ship noise. Sixteen narwhals were live captured and instrumented with satellite tags and Acousonde acoustic-behavioral recorders, and 11 of them were exposed to airgun pulses and vessel sounds. The sound exposure levels (SELs) of pulses from a small airgun (3.4 L) used in 2017 and a larger one (17.0 L) used in 2018 were measured using drifting recorders. The experiment was divided into trials with airgun and ship-noise exposure, intertrials with only ship-noise, and pre- and postexposure periods. Both trials and intertrials lasted ∼4 h on average per individual. Depending on the location of the whales, the number of separate exposures ranged between one and eight trials or intertrials. Received pulse SELs dropped below 130 dB re 1 μPa2 s by 2.5 km for the small airgun and 4–9 km for the larger airgun, and background noise levels were reached at distances of ∼3 and 8–10.5 km, respectively, for the small and big airguns. Avoidance reactions of the whales could be detected at distances &gt;5 km in 2017 and &gt;11 km in 2018 when in line of sight of the seismic vessel. Meanwhile, a ∼30% increase in horizontal travel speed could be detected up to 2 h before the seismic vessel was in line of sight. Applying line of sight as the criterion for exposure thus excludes some potential pre-response effects, and our estimates of effects must therefore be considered conservative. The whales reacted by changing their swimming speed and direction at distances between 5 and 24 km depending on topographical surroundings where the exposure occurred. The propensity of the whales to move towards the shore increased with increasing exposure (i.e., shorter distance to vessels) and was highest with the large airgun used in 2018, where the whales moved towards the shore at distances of 10–15 km. No long-term effects of the response study could be detected.

scholarly journals Long-term reproductive impairment in a seabird after the Prestige oil spill

2014 ◽  
Vol 10 (4) ◽  
pp. 20131041 ◽  
Author(s):  
Álvaro Barros ◽  
David Álvarez ◽  
Alberto Velando
Keyword(s):  
Oil Spill ◽  
Oil Spills ◽  
Population Level ◽  
Marine Organisms ◽  
Supporting Evidence ◽  
Long Term Effects ◽  
Reproductive Impairment ◽  
Long Term Consequences ◽  
The Impact

Large oil spills are dramatic perturbations on marine ecosystems, and seabirds are one of the worst affected organisms in such events. It has been argued that oil spills may have important long-term consequences on marine organisms, but supporting evidence remains scarce. The European shag ( Phalacrocorax aristotelis ) was strongly impacted at population level by the Prestige oil spill, the biggest spillage in the eastern North Atlantic. In this paper, we report on the long-term consequences on reproduction of this coastal seabird, using temporal and spatial replicated data (before–after–control–impact design). Our study revealed long-term reproductive impairment during at least the first 10 years since the Prestige oil spill. Annual reproductive success did not differ before the impact, but after the impact it was reduced by 45% in oiled colonies compared with unoiled ones. This is a rare documentation of long-term effects after a major oil spill, highlighting the need for long-term monitoring in order to assess the real impact of this type of disturbance on marine organisms.

scholarly journals Long term exposure of marine mussels to paracetamol: is time a healer or a killer?

2021 ◽  
Author(s):  
Wulan Koagouw ◽  
Nicolas A. Stewart ◽  
Corina Ciocan
Keyword(s):  
Chronic Exposure ◽  
Target Genes ◽  
Blue Mussel ◽  
Exposure Period ◽  
Neutral Red ◽  
Marine Organisms ◽  
Short Exposure ◽  
Toxicological Effects ◽  
Short Term Exposure

Abstract Pharmaceuticals pose a major threat to the marine environment and several studies have recently described their negative effects on marine organisms. Pharmaceutical compounds are constantly being released into aquatic ecosystems and chronic exposure, even at low concentrations, may have impact on marine organisms. The purpose of the present study is to evaluate the biological changes induced by one of the most widely used pharmaceuticals – paracetamol – in the blue mussel Mytilus edulis, after a long-term exposure at environmentally relevant concentrations. We present our data alongside and in comparison with results from a previous short-term exposure, to demonstrate the significance of exposure period on the effects of paracetamol in adult blue mussels. After 24 days of laboratory exposure, seven potential target genes were selected to examine toxicological effects in mussels’ gonads and possible disruptive effects on reproductive processes. The results show the modulation of some important reproduction-related genes: estrogen receptor-2 (ER2), vitelline envelope zona pellucida domain-9 (V9) and vitellogenin (VTG). Variations in mRNA expression of four other genes involved in apoptosis (HSP70, CASP8, BCL2 and FAS) are also highlighted. Histopathological alterations caused by paracetamol, together with neutral red retention time response in mussels’ hemocytes, are presented herein. Overall, this study highlights the exacerbated effects of low concentration of paracetamol after chronic exposure, similar to the damage induced by higher concentrations in a short exposure scenario, thus emphasizing the importance of length of exposure period when studying the effects of this substance. Additionally, this study also discusses the potential of paracetamol to inflict several major changes on the reproductive system of mussels and thus possibly affect the survival of populations.

136 Effects of the homology direct repair enhancer RS-1 on pig embryo culture

2020 ◽  
Vol 32 (2) ◽  
pp. 194
Author(s):  
C. Gomes Lucas ◽  
B. Bauer ◽  
P. Chen ◽  
L. Spate ◽  
K. Wells ◽  
...  
Keyword(s):  
Embryo Development ◽  
Embryo Culture ◽  
Statistical Significance ◽  
Mineral Oil ◽  
Cell Number ◽  
Long Term Effects ◽  
Control Groups ◽  
Short Term Exposure ◽  
And Control

Advances in genome editing technologies, such as the CRISPR/Cas9 system, have facilitated gene manipulation and the generation of pigs resistant to disease, models for studying human diseases and for xenotransplantation. However, double-strand breaks generated by the CRISPR/Cas9 system are preferentially repaired by the non-homologous end joining (NHEJ) pathway compared with high-fidelity homology-directed repair (HDR). All reports of pigs created by zygote injection of the CRISPR/Cas9 system result from NHEJ rather than HDR. The molecule known as RS-1 was found to stimulate RAD51 and thus enhance HDR. Thus, our goals are to understand this pathway by evaluating the response, as well as dosage and temporal effects, of RS-1 on porcine embryo development, and to determine a safe concentration to achieve high HDR rates without affecting embryo development. As RS-1 was added to culture medium from a 7.5mM stock solution in DMSO, we also evaluated whether RS-1 in solution could have affinity and migrate to the mineral oil overlay placed in the media during embryo culture. After IVF, embryos were cultured without RS-1 (control groups) or in the presence of RS-1 at 7.5 and 15 µM. At 7.5 µM, RS-1 improved HDR in rabbits created by zygote injection of the CRISPR/Cas9. Based on that, we designed five experimental groups to evaluate short- (A, 20h) and long-term (B, 144 h) effects of RS-1: two groups in the presence of RS-1 at 7.5 µM (7.5 µM A and 7.5 µM B), two control groups (control A and control B), and a group in the presence of RS-1 at 15 µM A for short-term exposure only. First, embryos in the groups containing (7.5 µM A, 7.5 µM B, and 15 µM A) and without RS-1 (control A and control B) were cultured for 20h without the mineral oil overlay. Then, embryos from control A, 7.5 µM A, and 15 µM A groups were washed and cultured without RS-1 until Day 6 in the presence of the mineral oil overlay. To evaluate long-term effects of RS-1, embryos from control B and 7.5 µM B groups were washed and transferred to medium without or with RS-1 at 7.5 µM, respectively, and cultured until Day 6 with no mineral oil overlay. We report the mean values for each set of data±s.e.m., and the degree of statistical significance in all analyses was defined at P&lt;0.05. We observed that the presence of RS-1 at 15 µM decreased cleavage rates (control A 79.1±2.7%; 7.5 µM A 83.6±1.6%; 15 µM 70.9±4.4%; control B 80.8±1.4%; 7.5 µM B 80.0±2.8%), and long-term exposure to RS-1 decreased development to the blastocyst stage (control A 46.5±3.0%; 7.5 µM A 42.7±3.4%; 15 µM 41.8±4.7%; control B 44.9±3.3%; 7.5 µM B 28.8±4.9%). In addition, RS-1 exposure decreased the total cell number compared with the controls (control A 61.4±2.8; 7.5 µM A 49.2±3.0; 15 µM 48.5±2.3; control B 59.9±3.4; 7.5 µM B 41.5±2.5), however no differences in apoptosis rates were observed between the treatments. Our work will serve as a basis to understand the effects of RS-1 and RAD51 during embryonic development and to improve the applicability of customizable nucleases for the production of genetically modified pigs.

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