scholarly journals Three-Dimensional Stable Alginate-Nanocellulose Gels for Biomedical Applications: Towards Tunable Mechanical Properties and Cell Growing

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 78 ◽  
Author(s):  
Priscila Siqueira ◽  
Éder Siqueira ◽  
Ana Elza De Lima ◽  
Gilberto Siqueira ◽  
Ana Delia Pinzón-Garcia ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Tissue Engineering ◽  
Wound Healing ◽  
Cellulose Nanocrystals ◽  
Biomedical Applications ◽  
Cellulose Nanofibers ◽  
Oxidized Cellulose ◽  
Freeze Dried ◽  
Alginate Gels

Hydrogels have been studied as promising materials in different biomedical applications such as cell culture in tissue engineering or in wound healing. In this work, we synthesized different nanocellulose-alginate hydrogels containing cellulose nanocrystals, TEMPO-oxidized cellulose nanocrystals (CNCTs), cellulose nanofibers or TEMPO-oxidized cellulose nanofibers (CNFTs). The hydrogels were freeze-dried and named as gels. The nanocelluloses and the gels were characterized by different techniques such as Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and dynamic mechanical thermal analysis (DMTA), while the biological features were characterized by cytotoxicity and cell growth assays. The addition of CNCTs or CNFTs in alginate gels contributed to the formation of porous structure (diameter of pores in the range between 40 and 150 μm). TEMPO-oxidized cellulose nanofibers have proven to play a crucial role in improving the dimensional stability of the samples when compared to the pure alginate gels, mainly after a thermal post-treatment of these gels containing 50 wt % of CNFT, which significantly increased the Ca2+ crosslinking density in the gel structure. The morphological characteristics, the mechanical properties, and the non-cytotoxic behavior of the CNFT-alginate gels improved bioadhesion, growth, and proliferation of the cells onto the gels. Thus, the alginate-nanocellulose gels might find applications in tissue engineering field, as for instance, in tissue repair or wound healing applications.

scholarly journals Three-dimensional Hydrogels of Alginate/chitosan Semi-interpenetrating Polymer Networks and Nanocelluloses

2021 ◽  
Author(s):  
Priscila Siqueira ◽  
Ana de Lima ◽  
Felipe Medeiros ◽  
Augusta Isaac ◽  
Katia Novack ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Dimensional Stability ◽  
Cellulose Nanocrystals ◽  
Biomedical Applications ◽  
Cellulose Nanofibers ◽  
Polymer Networks ◽  
Three Dimensional ◽  
Controlled Drug Release ◽  
Interpenetrating Polymer Networks ◽  
Post Treatment

Abstract The hydrogels are advanced materials used in biomedical applications during wound healing, controlled drug release and to prepare scaffolds. In this work are prepared hydrogels of alginate/chitosan (Alg/Ch) semi-interpenetrating polymer networks (semi-IPN’s) and nanocelluloses. The hydrogels after preparation by freeze drying are namely simply as gels. The cellulose nanocrystals (CNC’s) are obtained from acid hydrolysis of bleached Eucalyptus pulps and oxidized cellulose nanocrystals (CNCT’s) prepared by (2,2,6,6-tetramethylpiperidin-1-yl)oxyl radical catalyzed reaction as known as TEMPO reaction. The cellulose nanofibers (NFC’s) are obtained from mechanical shearing of cellulose pulps and oxidized NFC’s by TEMPO-mediated reaction (NFCT’s). The nanocellulose suspension and gels are characterized by FTIR at ATR mode, TGA, XRD, TEM, SEM, X-ray computed microtomography (micro-CT) and DMTA. The addition of CNC’s, NFC’s, CNCT’s or NFCT’s in the microstructure of gels increases their dimensional stabilities. The best results are obtained when CNCT’s and NFCT’s are added. The mechanical properties and dimensional stability of Alg/Ch semi-IPN’s increase after controlled thermal post-treatment. The heating during thermal post-treatment boosts the physicochemical interactions in the microstructures of semi-IPN’s. The biological assays show biocompatibility of fibroblast cells on the substrates, and differentiation and proliferation up seven days. The optimized mechanical properties, dimensional stability and biocompatibility of the gels studied in this work are important parameters for potential biomedical applications of these biomaterials.

scholarly journals Surface Modification of Bacterial Cellulose for Biomedical Applications

2022 ◽  
Vol 23 (2) ◽  
pp. 610
Author(s):  
Teresa Aditya ◽  
Jean Paul Allain ◽  
Camilo Jaramillo ◽  
Andrea Mesa Restrepo
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Surface Modification ◽  
Bacterial Cellulose ◽  
Biomedical Applications ◽  
Human Tissues ◽  
Naturally Occurring ◽  
Microstructure And Mechanical Properties

Bacterial cellulose is a naturally occurring polysaccharide with numerous biomedical applications that range from drug delivery platforms to tissue engineering strategies. BC possesses remarkable biocompatibility, microstructure, and mechanical properties that resemble native human tissues, making it suitable for the replacement of damaged or injured tissues. In this review, we will discuss the structure and mechanical properties of the BC and summarize the techniques used to characterize these properties. We will also discuss the functionalization of BC to yield nanocomposites and the surface modification of BC by plasma and irradiation-based methods to fabricate materials with improved functionalities such as bactericidal capabilities.

The Structure and the Mechanical Properties of a Newly Fabricated Cellulose-Nanofiber/Polyvinyl-Alcohol Composite

2014 ◽  
Vol 1621 ◽  
pp. 149-154
Author(s):  
Yukako Oishi ◽  
Atsushi Hotta
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Cellulose Nanofibers ◽  
Small Diameter ◽  
Short Fiber ◽  
Cellulose Nanofiber ◽  
Process Conditions ◽  
Aspect Ratios

ABSTRACTCellulose nanofibers (Cel-F) were extracted by a simple and harmless Star Burst (SB) method, which produced aqueous cellulose-nanofiber solution just by running original cellulose beads under a high pressure of water in the synthetic SB chamber. By optimizing the SB process conditions, the cellulose nanofibers with high aspect ratios and the small diameter of ∼23 nm were obtained, which was confirmed by transmission electron microscopy (TEM). From the structural analysis of the Cel-F/PVA composite by the scanning electron microscopy (SEM), it was found that the Cel-F were homogeneously dispersed in the PVA matrix. Considering the high molecular compatibility of the cellulose and PVA due to the hydrogen bonding, a good adhesive interface could be expected for the Cel-F and the PVA matrix. The influences of the morphological change in Cel-F on the mechanical properties of the composites were analysed. The Young’s modulus rapidly increased from 2.2 GPa to 2.9 GPa up to 40 SB treatments (represented by the unit Pass), whereas the Young’s modulus remained virtually constant above 40 Pass. Due to the uniform dispersibility of the Cel-F, the Young’s modulus of the 100 Pass composite at the concentration of 5 wt% increased up to 3.2 GPa. The experimental results corresponded well with the general theory of the composites with dispersed short-fiber fillers, which clearly indicated that the potential of the cellulose nanofibers as reinforcement materials for hydrophilic polymers was sufficiently confirmed.

scholarly journals Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering

2017 ◽  
Vol 46 (4) ◽  
pp. 691-705 ◽  
Author(s):  
Azizeh Rahmani Del Bakhshayesh ◽  
Nasim Annabi ◽  
Rovshan Khalilov ◽  
Abolfazl Akbarzadeh ◽  
Mohammad Samiei ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Biomedical Applications ◽  
Dermal Tissue ◽  
Recent Advances

scholarly journals Alginate/Gelatin Hydrogels Reinforced with TiO2 and β-TCP Fabricated by Microextrusion-based Printing for Tissue Regeneration

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 457 ◽  
Author(s):  
Rodrigo Urruela-Barrios ◽  
Erick Ramírez-Cedillo ◽  
A. Díaz de León ◽  
Alejandro Alvarez ◽  
Wendy Ortega-Lara
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Average Pore Size ◽  
Three Dimensional ◽  
Engineering Applications ◽  
Average Pore ◽  
Viscous Deformation ◽  
Freeze Dried ◽  
3D Printed

Three-dimensional (3D) printing technologies have become an attractive manufacturing process to fabricate scaffolds in tissue engineering. Recent research has focused on the fabrication of alginate complex shaped structures that closely mimic biological organs or tissues. Alginates can be effectively manufactured into porous three-dimensional networks for tissue engineering applications. However, the structure, mechanical properties, and shape fidelity of 3D-printed alginate hydrogels used for preparing tissue-engineered scaffolds is difficult to control. In this work, the use of alginate/gelatin hydrogels reinforced with TiO2 and β-tricalcium phosphate was studied to tailor the mechanical properties of 3D-printed hydrogels. The hydrogels reinforced with TiO2 and β-TCP showed enhanced mechanical properties up to 20 MPa of elastic modulus. Furthermore, the pores of the crosslinked printed structures were measured with an average pore size of 200 μm. Additionally, it was found that as more layers of the design were printed, there was an increase of the line width of the bottom layers due to its viscous deformation. Shrinkage of the design when the hydrogel is crosslinked and freeze dried was also measured and found to be up to 27% from the printed design. Overall, the proposed approach enabled fabrication of 3D-printed alginate scaffolds with adequate physical properties for tissue engineering applications.

scholarly journals Recent advances on polydiacetylene-based smart materials for biomedical applications

2020 ◽  
Vol 4 (4) ◽  
pp. 1089-1104 ◽  
Author(s):  
Fang Fang ◽  
Fanling Meng ◽  
Liang Luo
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Smart Materials ◽  
Biomedical Applications ◽  
Recent Advances ◽  
Smart Biomaterials

This review summarized most recent advances of designing strategies of polydiacetylene-based smart biomaterials with unique colorimetric and mechanical properties, as well as their applications in biosensing, drug delivery, and tissue engineering.

scholarly journals Use of Cellulose and Oxidized Cellulose Nanocrystals from Olive Stones in Chitosan Bionanocomposites

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Ragab E. Abou-Zeid ◽  
Enas A. Hassan ◽  
Fedia Bettaieb ◽  
Ramzi Khiari ◽  
Mohammad L. Hassan
Keyword(s):  
Electron Microscopy ◽  
Tensile Strength ◽  
Cellulose Nanocrystals ◽  
Strength Properties ◽  
Oxidized Cellulose ◽  
X Ray ◽  
Rate Of Dissolution ◽  
Olive Stones ◽  
Carboxylic Groups

Cellulose nanocrystals (CNC) and 2,2,6,6-tetramethyl-1-piperidinyloxyl- (TEMPO-) oxidized cellulose nanocrystals (CNC-TEMPO) were prepared from olive stones. The prepared nanocrystals were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and carboxylic groups content determination. The prepared nanocrystals were used as reinforcing elements in chitosan nanocomposites, which were characterized using X-ray diffraction (XRD) and tensile strength properties. In addition, the bioactivity of the prepared chitosan nanocomposites was studiedin vitroin simulated body fluid (SBF) using scanning electron microscopy (SEM) and electron diffraction X-ray spectroscopy (EDX). The results showed positive effect of the nanocrystals on tensile strength properties of chitosan and noticeable reduction in its rate of dissolution in SBF due to presence of cellulose nanocrystals. Chitosan nanocomposites containing CNC-TEMPO showed higher tensile strength properties and higher rate of dissolution in SBF than those containing cellulose nanocrystals. Nanocomposites containing CNC or CNC-TEMPO could not form significant amounts of hydroxyapatite (HAp) upon immersion in SBF for up to 4 weeks. Upon addition of nanohydroxyapatite to chitosan/cellulose nanocrystals films, formation of new hydroxyapatite depositions was observed. Presence of cellulose nanocrystals in chitosan/HAp resulted in formation and deposition of higher amounts of new HAp than in case of using chitosan/HAp without cellulose nanocrystals.

scholarly journals Antibacterial cellulose-based aerogels for wound healing application: A review

2020 ◽  
Vol 7 (10) ◽  
pp. 4032-4040
Author(s):  
Esam Bashir Yahya ◽  
Marwa Mohammed Alzalouk ◽  
Khalifa A. Alfallous ◽  
Abdullah F. Abogmaza
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Wound Healing ◽  
Wound Dressing ◽  
Biomedical Applications ◽  
Inorganic Materials ◽  
Medical Applications ◽  
Tissue Engineering Scaffolds

Aerogels have been steadily developed since its first invention to become one of the most promising materials for various medical and non-medical applications. It has been prepared from organic and inorganic materials, in pure forms or composites. Cellulose-based aerogels are considered one of the promising materials in biomedical applications due to their availability, degradability, biocompatibility and non-cytotoxicity compared to conventional silica or metal-based aerogels. The unique properties of such materials permit their utilization in drug delivery, biosensing, tissue engineering scaffolds, and wound dressing. This review presents a summary of aerogel development as well as the properties and applications of aerogels. Herein, we further discuss the recent works pertaining to utilization of cellulose-based aerogels for antibacterial delivery.

Recent advances of self-assembling peptide-based hydrogels for biomedical applications

Soft Matter ◽  
2019 ◽  
Vol 15 (8) ◽  
pp. 1704-1715 ◽  
Author(s):  
Jieling Li ◽  
Ruirui Xing ◽  
Shuo Bai ◽  
Xuehai Yan
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Wound Healing ◽  
Biomedical Applications ◽  
3D Bioprinting ◽  
Biological Applications ◽  
Antitumor Therapy ◽  
Self Assembling ◽  
Recent Advances

The review introduces several methods for fabrication of robust peptide-based hydrogels and their biological applications in the fields of drug delivery and antitumor therapy, antimicrobial and wound healing materials, and 3D bioprinting and tissue engineering.

Collagen Based Materials for Biomedical Applications: Preparation and Properties

2012 ◽  
Vol 706-709 ◽  
pp. 595-599 ◽  
Author(s):  
Alina Sionkowska
Keyword(s):  
Thin Films ◽  
Mechanical Properties ◽  
Tissue Engineering ◽  
Biomedical Applications ◽  
Synthetic Polymer ◽  
Cross Linking ◽  
Microstructure And Mechanical Properties

Collagen-based materials were prepared and their properties were studied. The shape of collagen materials was as follows: thin films, hydrogels, and sponges. Microstructure and mechanical properties of films and sponges were studied. The effect of cross-linking agents and the effect of synthetic polymer on the properties of collagen materials were studied and analyzed. Collagen-based materials can be considered as potential biomaterials in tissue engineering.

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