scholarly journals Optimization of cultivation conditions of Salvia viridis L. shoots in the Plantform bioreactor to increase polyphenol production

2021 ◽  
Author(s):  
Izabela Grzegorczyk-Karolak ◽  
Paulina Staniewska ◽  
Liwia Lebelt ◽  
Dorota G. Piotrowska
Keyword(s):  
Phenolic Acid ◽  
Large Scale ◽  
Growth Parameters ◽  
Shoot Culture ◽  
Continuous System ◽  
Dry Weight ◽  
Total Phenolic ◽  
Efficient Production ◽  
Anticancer Activities ◽  
Batch System

AbstractSalvia viridis is a rich source of pharmacologically-important polyphenolic compounds with anti-inflammatory, antioxidant and anticancer activities. The aim of the present study was to optimise the large-scale cultivation of sage shoots in the Plantform bioreactor. It evaluated the effect of culture duration (two or three weeks), the use of a semi-continuous system and the immersion frequency in a liquid medium (every 80 or 170 min) on the growth and accumulation of secondary metabolites. The content of bioactive phenolic acids and phenylethanoids in the obtained material was determined using HPLC. The most efficient growth parameters, expressed as fresh (26.99 g) and dry weight (3.51 g) as well as proliferation ratio (18.6), were observed for shoots grown in a fed-batch system, immersed every 80 min; this approach yielded a 33-fold biomass increase within four weeks. However, the most efficient production of bioactive compounds was achieved for shoots grown for three weeks in a batch system, immersed every 80 min: total phenolic acid, total phenylethanoid and total phenol contents of 18.3, 11.4 and 29.7 mg/g DW. In these conditions, within three weeks, total phenolic acid level was almost 10 times higher than that found in the aerial parts of four-month-old soil-grown plants with a similar phenylethanoid level. Hence, the described S. viridis shoot culture cultivated in the Plantform bioreactor may be an alternative efficient source of plant material rich in valuable compounds.

scholarly journals Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Bubble Column ◽  
Growth Parameters ◽  
Growth Index ◽  
Dry Weight ◽  
Growth Conditions ◽  
Good Growth ◽  
Gentiana Dinarica ◽  
Hairy Root Clones

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

scholarly journals In VitroAntioxidant and Anticancer Activity of Flavonoid Fraction from the Aerial Parts ofCissus quadrangularisLinn. against Human Breast Carcinoma Cell Lines

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
A. Vijayalakshmi ◽  
P. R. Kumar ◽  
S. Sakthi Priyadarsini ◽  
C. Meenaxshi
Keyword(s):  
Breast Cancer ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Total Phenolic ◽  
Anticancer Activities ◽  
Aerial Parts ◽  
Cissus Quadrangularis ◽  
Flavonoid Fraction

Aim. The present study aimed to isolate flavonoid fraction from the aerial parts ofCissus quadrangularisand to evaluate its antioxidant and anticancer potential usingin vitroassay system.Methods. Total phenolic and flavonoid contents were calculated for the drug. Flavonoid fraction was isolated using column chromatography and analysed using HPLC.In vitro, antioxidant activity of the ethanol extract and isolated flavonoid fraction was investigated by nitric oxide, DPPH and hydroxyl radical scavenging assays. Breast cancer (MCF 7) cell line was used as thein vitrocancer model for MTT assay.Result. The amount of total phenolic content and total flavonoid content in the ethanol extract showed 28.6 mg/g dry weight expressed as gallic acid equivalents, and 15.8 mg/g was expressed as quercetin equivalents, respectively. The tested extract showed good dose-dependent free radical scavenging property in all the models with the IC50values of 98 μg/mL, 125 μg/mL, and 96 μg/mL for ethanol extract and 10 μg/mL, 12 μg/mL, and 10 μg/mL for flavonoid fraction, respectively. The flavonoid fraction possess potent anticancer property against breast cancer cells (MCF7) with IC50value of 40 μg/mL.Conclusions. It can be concluded that the aerial part ofCissus quadrangularishas potential antioxidant and anticancer activities.

scholarly journals Accumulation of Phenolic Acids during Storage over Differently Handled Fresh Carrots

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1515
Author(s):  
Jarkko Hellström ◽  
Daniel Granato ◽  
Pirjo H. Mattila
Keyword(s):  
Phenolic Acids ◽  
Phenolic Acid ◽  
High Resolution Mass Spectrometry ◽  
Storage Period ◽  
Dry Weight ◽  
Total Phenolic ◽  
Case Processing ◽  
Minimal Processing ◽  
Technological Processing ◽  
Selection Of

Carrots contain a significant content of phenolic compounds, mainly phenolic acids. Technological processing of carrots inflicts wounding stress and induces accumulation of these compounds, especially caffeic acid derivatives, in the periderm tissue. In this study, the effect of minimal processing (polishing, washing, peeling, and grating) on the retention of soluble phenolic acids in carrots was monitored during cold storage. Storage for up to 4 weeks and 24 h was used for whole and grated carrot samples, respectively. Total phenolic acid levels found in differently processed carrots varied greatly at the beginning of the storage period and on dry weight basis they ranged from 228 ± 67.9 mg/kg (grated carrot) to 996 ± 177 mg/kg (machine washed). In each case, processing followed by storage induced phenolic acid accumulation in the carrots. At the end of the experiment (4 weeks at +8 °C), untreated and machine-washed carrots contained ca. 4-fold more phenolic acids than at day 0. Similarly, polished carrots contained 9-fold and peeled carrots 31-fold more phenolic acids than at day 0. The phenolic acid content in grated carrot doubled after 24 h storage at +4 °C. Individual phenolic acids were characterized by high resolution mass spectrometry. MS data strongly suggest the presence of daucic acid conjugates of phenolic acids in carrot. Storage time did not have statistically similar effect on all compounds and generally in a way that dicaffeoyldaucic acid had the highest increase. This research provides important information for primary production, packaging, catering, the fresh-cut industry and consumers regarding the selection of healthier minimally processed carrots.

scholarly journals The Influence of Nasturtium officinale R. Br. Agar and Agitated Microshoot Culture Media on Glucosinolate and Phenolic Acid Production, and Antioxidant Activity

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1216 ◽  
Author(s):  
Marta Klimek-Szczykutowicz ◽  
Agnieszka Szopa ◽  
Michał Dziurka ◽  
Łukasz Komsta ◽  
Michał Tomczyk ◽  
...  
Keyword(s):  
Phenolic Acid ◽  
Total Phenolic Content ◽  
Acid Production ◽  
Phenolic Content ◽  
Culture Media ◽  
Dry Weight ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Nasturtium Officinale ◽  
2,4 Dichlorophenoxyacetic Acid

This paper presents an optimization of conditions for microshoot cultures of Nasturtium officinale R. Br. (watercress). Variants of the Murashige and Skoog (MS) medium containing different plant growth regulators (PGRs): cytokinins—BA (6-benzyladenine), 2iP (6-γ,γ-dimethylallylaminopurine), KIN (kinetin), Zea (zeatin), and auxins—IAA (3-indoleacetic acid), IBA (indole-3-butyric acid), 2,4-d (2,4-dichlorophenoxyacetic acid), IPA (indole-3-pyruvic acid), NAA (naphthalene-1-acetic acid), total 27 MS variants, were tested in agar and agitated cultures. Growth cycles were tested for 10, 20, or 30 days in the agar cultures, and 10 or 20 days in the agitated cultures. Glucosinolate and phenolic acid production, total phenolic content and antioxidant potential were evaluated. The total amounts of glucosinolates ranged from 100.23 to 194.77 mg/100 g dry weight of biomass (DW) in agar cultures, and from 78.09 to 182.80 mg/100 g DW in agitated cultures. The total phenolic acid content varied from 15.89 to 237.52 mg/100 g DW for the agar cultures, and from 70.80 to 236.74 mg/100 g DW for the agitated cultures. Extracts of the cultured biomass contained higher total amounts of phenolic acids, lower total amounts of glucosinolates, a higher total phenolic content and similar antioxidant potentials compared to plant material. The analyses performed confirmed for the first time the explicit influence on secondary metabolite production and on the antioxidant potential. The significance was statistically estimated in a complex manner.

scholarly journals Growth of Basil (Ocimum basilicum) in Aeroponics, DRF, and Raft Systems with Effluents of African Catfish (Clarias gariepinus) in Decoupled Aquaponics (s.s.)

2021 ◽  
Vol 3 (3) ◽  
pp. 559-574
Author(s):  
Johannes Pasch ◽  
Samuel Appelbaum ◽  
Harry Wilhelm Palm ◽  
Ulrich Knaus
Keyword(s):  
Large Scale ◽  
Growth Parameters ◽  
Clarias Gariepinus ◽  
Dry Weight ◽  
Ocimum Basilicum ◽  
African Catfish ◽  
Floating Raft ◽  
Tropical Conditions ◽  
Intensive Rearing ◽  
System Improvement

Basil (Ocimum basilicum) was cultivated in three hydroponic subsystems (i) a modified commercial aeroponics, (ii) a dynamic root floating (DRF) system, and (iii) a floating raft system in a decoupled aquaponic system in Northern Germany, Mecklenburg–Western Pomerania. For plant nutrition, aquaculture process water from intensive rearing of African catfish (Clarias gariepinus) was used without fertilizer. After 39 days, 16 plant growth parameters were compared, with aeroponics performing significantly better in 11 parameters compared with the DRF, and better compared with the raft in 13 parameters. The economically important leaf wet and dry weight was over 40% higher in aeroponics (28.53 ± 8.74 g; 4.26 ± 1.23 g), but similar in the DRF (20.19 ± 6.57 g; 2.83 ± 0.90 g) and raft (20.35 ± 7.14 g; 2.84 ± 1.04 g). The roots in the DRF grew shorter and thicker; however, this resulted in a higher root dry weight in aeroponics (1.08 ± 0.38 g) compared with the DRF (0.82 ± 0.36 g) and raft (0.67 ± 0.27 g). With optimal fertilizer and system improvement, aquaponic aeroponics (s.s.) could become a productive and sustainable large-scale food production system in the future. Due to its simple construction, the raft is ideal for domestic or semi-commercial use and can be used in areas where water is neither scarce nor expensive. The DRF system is particularly suitable for basil cultivation under hot tropical conditions.

scholarly journals Hepatoprotective, Antioxidant, and Anticancer Effects of theTragopogon porrifoliusMethanolic Extract

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Clara Tenkerian ◽  
Mirvat El-Sibai ◽  
Costantine F. Daher ◽  
Mohamad Mroueh
Keyword(s):  
Antioxidant Activity ◽  
Liver Toxicity ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Fold Increase ◽  
Dry Weight ◽  
Total Phenolic ◽  
Anticancer Activities

Tragopogon porrifolius(Asteraceae), commonly referred to as white salsify, is an edible herb used in Lebanese folk medicine to treat cancer and liver dysfunction. In this study, we investigated the antioxidant activity ofTragopogon porrifoliusmethanolic extract, bothin vitroandin vivo, in addition to its hepatoprotective and anticancer activities. Total phenolic and flavonoid contents were measured and found to be37.0±1.40 mg GAE/g and16.6±0.42 mg QE/g dry weight, respectively.In vitroantioxidant assays revealed an FRAP value of659±13.8 µmol Fe2+/g of extract and DPPH IC50value 15.2 µg/mL. In rats subjected to CCl4-induced hepatotoxicity, significant increase in CAT, SOD, and GST levels was detected. The highest dose of the extract (250 mg/kg) recorded a fold increase of 1.68 for SOD, 2.49 for GST, and 3.2 for CAT. The extract also showed substantial decrease in AST (57%), ALT (56%), and LDH (65%) levels. Additionally, the extract caused a dose-dependent decrease in cell viability and proliferation. In conclusion, the methanolic extract ofT. porrifoliusdisplayed a relatively high antioxidant activity bothin vitroandin vivoas well as hepatoprotective potential against liver toxicity in rats and anticancer effect on MDA-MB-231 and Caco-2 cells.

scholarly journals Antioxidant Activity and Phenolic Composition in ‘Beauregard’ Sweetpotato Are Affected by Root Size and Leaf Age

2007 ◽  
Vol 132 (4) ◽  
pp. 447-451 ◽  
Author(s):  
Malkeet S. Padda ◽  
D.H. Picha
Keyword(s):  
Antioxidant Activity ◽  
Chlorogenic Acid ◽  
Phenolic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Leaf Age ◽  
Dry Weight ◽  
Total Phenolic ◽  
Phenolic Antioxidants ◽  
Root Weight

Phenolic acids are considered important antioxidants that may help to prevent many human chronic diseases. The antioxidant activity and phenolic content of sweetpotato [Ipomoea batatas (L.) Lam.] roots and leaves of different sizes and ages, respectively, were quantified. Small roots (≈4 g root weight) had a higher antioxidant activity and phenolic content compared with full-sized marketable roots (≈300 g root weight). Phenolic content in marketable roots was significantly higher in the cortex tissue than in the internal pith tissue. The highest total phenolic content [chlorogenic acid equivalents (10.3 mg·g−1 dry weight)] and antioxidant activity [Trolox equivalents (9.7 mg·g−1 dry weight)] was found in cortex tissue of small-sized roots. Sweetpotato leaves had a significantly higher phenolic content and antioxidant activity than roots. Young, immature unfolded leaves had the highest total phenolic content (88.5 mg·g−1 dry weight) and antioxidant activity (99.6 mg·g−1 dry weight). Chlorogenic acid was the major phenolic acid in root and leaf tissues with the exception of young immature leaves in which the predominant phenolic acid was 3,5-dicaffeoylquinic acid. The results suggest that small-sized roots, which are typically discarded in the field, and young immature leaves may be concentrated sources of phenolic antioxidants.

scholarly journals Effect of container and potting media on raising quality seedlings of Acacia auriculiformis in the nursery

2019 ◽  
Vol 3 (01) ◽  
Author(s):  
MD. ARIFUL ISLAM ◽  
RAYHANUR RAHMAN ◽  
MOHAMMED KAMAL HOSSAIN
Keyword(s):  
Root Length ◽  
Quality Index ◽  
Large Scale ◽  
Growth Parameters ◽  
Block Design ◽  
Dry Weight ◽  
Acacia Auriculiformis ◽  
Cow Dung ◽  
Randomized Block Design ◽  
Potting Media

Abstract. Islam MDA, Rahman MDA, Hossain MK. 2019. Effect of container and potting media on raising quality seedlings of Acacia auriculiformis in the nursery. Asian J Agric 3: 26-32. The study elucidates the effect of container and potting media on raising quality seedlings of Acacia auriculiformis in the nursery of the Institute of Forestry and Environmental Sciences, University of Chittagong to find out a suitable container and potting media for raising large scale quality seedlings. The seedlings were evaluated by five container and seven potting media treatments for eight months. A Completely Randomized Block Design (CRBD) was adopted for the study with three replications for each treatment. The Analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) was tested for the analysis to explore the possible treatment variations. However, the study reveals nodulation with growth parameters; shoot and root dry biomass production and quality index were highest in 20 cm × 15 cm size polybags whereas the highest root length and shoot-root ratio was observed in 15 cm × 10 cm and 15 cm × 13 cm size polybag respectively. Considering the potting media, highest nodulation, growth parameters, shoot dry and fresh weight, shoot- root ratio and biomass were found in combination of soil + cow dung + phosphorus (0.16 g/polybag). Highest root length, root fresh and dry weight, and quality index were found in the combination of soil + cow dung (3:1). Therefore, it is recommended that containers of 20 cm × 15 cm size polybag and with a potting media of soil + cow dung + phosphorus (3 parts soil, 1 parts cow dung + 0.16 g/polybag) combination produce quality A. auriculiformis seedlings in the nursery.

Salt stress enhanced antioxidant response in callus of three halophytes (Salsola baryosma, Trianthema triquetra, Zygophyllum simplex) of Thar Desert

Biologia ◽  
2014 ◽  
Vol 69 (2) ◽  
Author(s):  
Varsha Sharma ◽  
Kishan Ramawat
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Capacity ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Elevated Concentration ◽  
Thar Desert ◽  
Frap Assay

AbstractEffects of salinity on growth, protein content, proline, catalase and antioxidant enzyme activity in callus of three halophytes of the Thar Desert; Salsola baryosma, Trianthema triquetra and Zygophyllum simplex were evaluated. Callus tissues were cultured on Murashige and Skoog’s medium containing different concentrations of NaCl (50, 100 and 200 mM). Increase in dry weight and soluble proteins were observed in the callus exposed to lower salinity (50 and 100 mM NaCl) in all the three species, whereas on the medium containing 200 mM NaCl, significant decrease in these two growth parameters was recorded. Under the salinity stress maximum proline accumulation was found in S. baryosma with parallel increase in soluble sugars. Among the three species, T. triquetra callus showed maximum CAT activity with 50 and 100 mM NaCl treatment, whereas the enzyme activity decreased at 200 mM NaCl treatment in all three species. The antioxidant potential steadily elevated under salt treatment in all the above three species using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant potential (FRAP) assay. Whereas, superoxide dismutase (SOD) quenching were recorded maximum at low (50 and 100 mM) concentrations in all the three species. However, T. triquetra callus showed maximum total phenolic content (TPC) 15 mg GAE g−1 with the elevated concentration of NaCl up to 200 mM, and S. baryosma callus showed lower TPC as compared to both species. A significant correlation between antioxidant capacity and TPC was observed indicating that phenolic compounds are the major contributors to the antioxidant potential in these halophyte species. FRAP and DPPH activity of Z. simplex showed maximum correlation (R = 0.992), as compared to other two species. We can conclude that all the three species exhibit a protection mechanism by sustaining growth parameters and antioxidant capacity. Due to high antioxidant property of all these species, the plant extracts may be included in nutraceutical formulations.

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Cochliobolus Sativus ◽  
Resistance Level ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Barley Genotypes

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

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